Rmu_co8340541.1_g000001

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8340541.1
OG0014180
Physical Location & Seq
Forward (+)
1 .. 561
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8340541.1_g000001.1.cds

Sequence Viewer

Length: 399 bp
ggtggatcagggtctgttcagggagtcctattctcaaacatccaagtcaatgaagttcaacttcctattgtgatagaccagttctactgtgacaaaagcacttgcagaaaccaatcatcagctgtggcgctctcaggaatcacctacgagagaattcgaggaacatacacagtaaaaccggtacattttgcctgcagtgacagcctaccctgcgtagacgtgacactaagcggcgtacagctaaagccagtgcaagaacgctatcacatgtatgatcccttctgctggcagactttcggggagttgaggaccccgacgctgccaccaattccgtgtttacagattggtaagccgccgaacaacaaggtcaattcggatcatgatgcgtgtgacctttag

Protein Analysis

132

Amino Acids

14.56

Weight (kDa)

5.84

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AciI CCGC 2 cut(s) 231, 353
AclWI GGATC 3 cut(s) 13, 269, 384
AcsI RAATTY 1 cut(s) 153
AfaI GTAC 2 cut(s) 183, 237
AfiI CCNNNNNNNGG 1 cut(s) 285
AflIII ACRYGT 1 cut(s) 267
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 1 cut(s) 59
AjiI CACGTC 1 cut(s) 220
AluBI AGCT 2 cut(s) 122, 241
AluI AGCT 2 cut(s) 122, 241
AlwI GGATC 3 cut(s) 13, 269, 384
AlwNI CAGNNNCTG 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 319
ApoI RAATTY 1 cut(s) 153
AsiGI ACCGGT 1 cut(s) 178
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 309
BarI GAAGNNNNNNTAC 2 cut(s) 263, 295
BbvI GCAGC 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 3 cut(s) 232, 320, 353
BlsI GCNGC 3 cut(s) 233, 321, 354
Bme18I GGWCC 1 cut(s) 309
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 311
BmsI GCATC 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 79, 248
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 2 cut(s) 79, 248
BseXI GCAGC 1 cut(s) 306
BshTI ACCGGT 1 cut(s) 178
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 285
Bsp143I GATC 3 cut(s) 5, 274, 376
BspACI CCGC 2 cut(s) 231, 353
BspCNI CTCAG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 197
BspPI GGATC 3 cut(s) 13, 269, 384
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 79, 248
BssAI RCCGGY 1 cut(s) 178
BssMI GATC 3 cut(s) 5, 274, 376
Bst4CI ACNGT 2 cut(s) 89, 172
BstC8I GCNNGC 2 cut(s) 193, 287
BstDEI CTNAG 2 cut(s) 133, 227
BstF5I GGATG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 8, 277, 379
BstMBI GATC 3 cut(s) 5, 274, 376
BstMWI GCNNNNNNNGC 2 cut(s) 201, 210
BstNSI RCATGY 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 306
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 202, 255
Cac8I GCNNGC 2 cut(s) 193, 287
CaiI CAGNNNCTG 1 cut(s) 14
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 309
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 182, 236
CspAI ACCGGT 1 cut(s) 178
CviAII CATG 2 cut(s) 268, 380
CviJI RGCY 5 cut(s) 122, 204, 241, 247, 352
CviKI_1 RGCY 5 cut(s) 122, 204, 241, 247, 352
CviQI GTAC 2 cut(s) 182, 236
DdeI CTNAG 2 cut(s) 133, 227
DpnI GATC 3 cut(s) 7, 276, 378
DpnII GATC 3 cut(s) 5, 274, 376
Eco47I GGWCC 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 153
FaeI CATG 2 cut(s) 271, 383
FaiI YATR 4 cut(s) 166, 269, 273, 381
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FatI CATG 2 cut(s) 267, 379
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 232, 320, 353
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 3 cut(s) 232, 320, 353
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 232, 320, 353
HaeII RGCGCY 1 cut(s) 131
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 325
HhaI GCGC 1 cut(s) 130
Hin1II CATG 2 cut(s) 271, 383
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 2 cut(s) 24, 138
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 217, 338
Hpy188I TCNGA 1 cut(s) 376
Hpy188III TCNNGA 2 cut(s) 135, 380
Hpy8I GTNNAC 2 cut(s) 217, 338
Hpy99I CGWCG 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 89, 172
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 105, 195, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 210
HpyF3I CTNAG 2 cut(s) 133, 227
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 2 cut(s) 271, 383
HspAI GCGC 1 cut(s) 128
Kzo9I GATC 3 cut(s) 5, 274, 376
LpnPI CCDG 8 cut(s) 5, 92, 120, 192, 205, 223, 261, 271
Lsp1109I GCAGC 1 cut(s) 306
LweI GCATC 1 cut(s) 373
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 4 cut(s) 89, 197, 220, 389
MalI GATC 3 cut(s) 7, 276, 378
MboI GATC 3 cut(s) 5, 274, 376
MluCI AATT 3 cut(s) 153, 327, 370
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 2 cut(s) 152, 300
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 201, 210
NdeII GATC 3 cut(s) 5, 274, 376
NlaIII CATG 2 cut(s) 271, 383
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 4 cut(s) 89, 197, 220, 389
NspI RCATGY 1 cut(s) 271
PagI TCATGA 1 cut(s) 379
PciI ACATGT 1 cut(s) 267
PfeI GAWTC 1 cut(s) 138
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 3 cut(s) 233, 321, 354
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PpuMI RGGWCCY 1 cut(s) 309
PscI ACATGT 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 309
PspPPI RGGWCCY 1 cut(s) 309
PstI CTGCAG 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 14
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 183, 237
RsaNI GTAC 2 cut(s) 182, 236
RseI CAYNNNNRTG 1 cut(s) 270
SatI GCNGC 3 cut(s) 232, 320, 353
Sau3AI GATC 3 cut(s) 5, 274, 376
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 1 cut(s) 33
SetI ASST 6 cut(s) 124, 146, 222, 243, 369, 396
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 1 cut(s) 309
SmiMI CAYNNNNRTG 1 cut(s) 270
Sse9I AATT 3 cut(s) 153, 327, 370
SsiI CCGC 2 cut(s) 231, 353
TaaI ACNGT 2 cut(s) 89, 172
TaiI ACGT 1 cut(s) 222
TaqI TCGA 1 cut(s) 157
TasI AATT 3 cut(s) 153, 327, 370
TauI GCSGC 2 cut(s) 234, 355
TfiI GAWTC 1 cut(s) 138
TscAI CASTG 2 cut(s) 202, 255
TseFI GTSAC 4 cut(s) 89, 197, 220, 389
TseI GCWGC 1 cut(s) 319
Tsp45I GTSAC 4 cut(s) 89, 197, 220, 389
TspDTI ATGAA 1 cut(s) 66
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 2 cut(s) 202, 255
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 1 cut(s) 153
XceI RCATGY 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.