AT1G12244

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016704
Physical Location & Seq
Forward (+)
4157654 .. 4159046
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12244.1

Sequence Viewer

Length: 765 bp
ATGGAACGAACTACTGTAATACTGTTCGTCTTCTTCTTGCTCTCTGTAGCAATGTGTTCTCTTCACTCTTTTTCTTCCGTAGCAATTTCATTCAGAACTCAAAAGTACGTAAATACCTCTCTCGTTCATCGCTTATCTCCTATAGGATATCGTAATTATCCTCTACCGTGGGCAGTGACGTCAATTGACGAAATCCCTCCTAATGCTGTTCGCCGGAAAATAGACCGGAATTGGAGAGGTGGGTTCAGTCTCGGCGTAGATTTAGGTTTATCTCGAACCGGAATCGCTATTAGTAAAGGCTACACCGTTAAACCTCTCACGGTTTTGAAATCGAGAGGGCAAAAACTCGAGACCAGGCTTTTAGAAATTGCTGAAGAAGAAGAGGCTGATGAATTTATAATTGGGTTGCCGAGATCTTCTGATGGGAAAGAGACGATTCAATCGAACAAAATTCGTTCTGTTGCTGGACGGCTCGCTATTCAAGCCGCCGAGAGGGGTTGGAGAGTTTATGTATTTGATGAACATGGAACAACGTCAGAAGCTTCAGATAGAATGATTGTAATGGGACTTAGCAAGAGTGAAAGACAGAACAGATCAGATGCATATGCTGCTGTGATATTGTTGGAGAGGTATTTCTCTACACAAGGTTTAGGAATTGAGATTATACTTCCCAAGAGTTTAGAGCTTCAAGCAAAGATCAAGAACGGTGCACCTGTAGACCCCGACTTCAATCCGGAAAACCTCGAAGAGTATTACACATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

254

Amino Acids

28.55

Weight (kDa)

8.57

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 84 - 213 8.3e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
AatII GACGTC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 717
AccIII TCCGGA 1 cut(s) 733
AciI CCGC 1 cut(s) 486
AcsI RAATTY 2 cut(s) 392, 450
AcuI CTGAAG 2 cut(s) 393, 528
AcyI GRCGYC 1 cut(s) 179
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 492
AgsI TTSAA 5 cut(s) 328, 440, 482, 689, 730
AjnI CCWGG 1 cut(s) 353
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 2 cut(s) 542, 685
AluI AGCT 2 cut(s) 542, 685
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 3 cut(s) 254, 344, 425
Alw44I GTGCAC 1 cut(s) 708
Ama87I CYCGRG 1 cut(s) 347
Aor13HI TCCGGA 1 cut(s) 733
ApaLI GTGCAC 1 cut(s) 708
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 2 cut(s) 392, 450
AvaI CYCGRG 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 712
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 1 cut(s) 595
BccI CCATC 1 cut(s) 416
BceAI ACGGC 1 cut(s) 485
BciT130I CCWGG 1 cut(s) 355
BcoDI GTCTC 3 cut(s) 254, 344, 425
BfmI CTRYAG 3 cut(s) 45, 141, 714
BglII AGATCT 1 cut(s) 413
BisI GCNGC 2 cut(s) 486, 609
BlsI GCNGC 2 cut(s) 487, 610
Bme1390I CCNGG 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 347
BmrFI CCNGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 589
BpiI GAAGAC 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 3 cut(s) 225, 278, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse3DI GCAATG 1 cut(s) 57
BseAI TCCGGA 1 cut(s) 733
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMI GCAATG 1 cut(s) 57
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 712
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 4 cut(s) 214, 226, 279, 734
BslFI GGGAC 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 3 cut(s) 254, 344, 425
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 579
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 712
Bsp13I TCCGGA 1 cut(s) 733
Bsp143I GATC 3 cut(s) 413, 593, 696
BspACI CCGC 1 cut(s) 486
BspEI TCCGGA 1 cut(s) 733
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 57
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 3 cut(s) 413, 593, 696
BssNI GRCGYC 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 6 cut(s) 16, 24, 168, 307, 322, 707
Bst6I CTCTTC 3 cut(s) 66, 375, 741
BstACI GRCGYC 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 608
BstBAI YACGTR 1 cut(s) 109
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 1 cut(s) 569
BstDSI CCRYGG 1 cut(s) 167
BstKTI GATC 3 cut(s) 416, 596, 699
BstMAI GTCTC 3 cut(s) 254, 344, 425
BstMBI GATC 3 cut(s) 413, 593, 696
BstMWI GCNNNNNNNGC 2 cut(s) 482, 608
BstNI CCWGG 1 cut(s) 355
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 3 cut(s) 45, 141, 714
BstSLI GKGCMC 1 cut(s) 712
BstSNI TACGTA 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BtgI CCRYGG 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 113
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 474
Csp6I GTAC 1 cut(s) 106
CviAII CATG 1 cut(s) 524
CviJI RGCY 7 cut(s) 300, 358, 386, 472, 485, 542, 685
CviKI_1 RGCY 7 cut(s) 300, 358, 386, 472, 485, 542, 685
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 569
DpnI GATC 3 cut(s) 415, 595, 698
DpnII GATC 3 cut(s) 413, 593, 696
Eam1104I CTCTTC 3 cut(s) 66, 375, 741
EarI CTCTTC 3 cut(s) 66, 375, 741
Eco105I TACGTA 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 344
Eco32I GATATC 1 cut(s) 149
Eco57I CTGAAG 2 cut(s) 393, 528
Eco88I CYCGRG 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 353
EcoRV GATATC 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 604
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 1 cut(s) 527
FaiI YATR 7 cut(s) 143, 398, 510, 525, 604, 606, 665
FaqI GGGAC 1 cut(s) 579
FatI CATG 1 cut(s) 523
FauNDI CATATG 1 cut(s) 604
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 2 cut(s) 486, 609
Fsp4HI GCNGC 2 cut(s) 486, 609
GluI GCNGC 2 cut(s) 486, 609
HapII CCGG 4 cut(s) 214, 226, 279, 734
Hin1I GRCGYC 1 cut(s) 179
Hin1II CATG 1 cut(s) 527
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 2 cut(s) 282, 436
HpaII CCGG 4 cut(s) 214, 226, 279, 734
Hpy166II GTNNAC 2 cut(s) 710, 718
Hpy188I TCNGA 5 cut(s) 95, 421, 538, 547, 598
Hpy188III TCNNGA 5 cut(s) 273, 333, 349, 700, 734
Hpy8I GTNNAC 2 cut(s) 710, 718
HpyCH4III ACNGT 6 cut(s) 16, 24, 168, 307, 322, 707
HpyCH4IV ACGT 3 cut(s) 108, 179, 533
HpyCH4V TGCA 2 cut(s) 602, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 608
HpyF3I CTNAG 1 cut(s) 569
HpySE526I ACGT 3 cut(s) 108, 179, 533
Hsp92I GRCGYC 1 cut(s) 179
Hsp92II CATG 1 cut(s) 527
Kpn2I TCCGGA 1 cut(s) 733
Kzo9I GATC 3 cut(s) 413, 593, 696
LpnPI CCDG 8 cut(s) 227, 239, 292, 340, 367, 450, 726, 747
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 1 cut(s) 589
MaeII ACGT 3 cut(s) 108, 179, 533
MaeIII GTNAC 1 cut(s) 175
MalI GATC 3 cut(s) 415, 595, 698
MboI GATC 3 cut(s) 413, 593, 696
MboII GAAGA 9 cut(s) 22, 25, 53, 66, 386, 389, 392, 408, 758
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 9 cut(s) 84, 154, 183, 229, 366, 392, 399, 450, 654
MmeI TCCRAC 2 cut(s) 479, 603
Mph1103I ATGCAT 1 cut(s) 604
MroI TCCGGA 1 cut(s) 733
MseI TTAA 1 cut(s) 309
MspI CCGG 4 cut(s) 214, 226, 279, 734
MspR9I CCNGG 1 cut(s) 355
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 355
MwoI GCNNNNNNNGC 2 cut(s) 482, 608
NdeI CATATG 1 cut(s) 604
NdeII GATC 3 cut(s) 413, 593, 696
NlaIII CATG 1 cut(s) 527
NmeAIII GCCGAG 3 cut(s) 231, 435, 514
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 604
PaeR7I CTCGAG 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PfeI GAWTC 2 cut(s) 282, 436
PkrI GCNGC 2 cut(s) 487, 610
Ppu21I YACGTR 1 cut(s) 109
PsiI TTATAA 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 486, 609
Sau3AI GATC 3 cut(s) 413, 593, 696
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 1 cut(s) 712
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 3 cut(s) 45, 141, 714
Sfr274I CTCGAG 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SnaBI TACGTA 1 cut(s) 109
Sse9I AATT 9 cut(s) 84, 154, 183, 229, 366, 392, 399, 450, 654
SsiI CCGC 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 353
TaaI ACNGT 6 cut(s) 16, 24, 168, 307, 322, 707
TaiI ACGT 3 cut(s) 111, 182, 536
TaqI TCGA 5 cut(s) 274, 332, 348, 443, 744
TasI AATT 9 cut(s) 84, 154, 183, 229, 366, 392, 399, 450, 654
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 2 cut(s) 282, 436
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 4 cut(s) 78, 116, 405, 534
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 180
VneI GTGCAC 1 cut(s) 708
XapI RAATTY 2 cut(s) 392, 450
XhoI CTCGAG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 717
ZraI GACGTC 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.