Rorug01G0199600

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016704
Physical Location & Seq
Forward (+)
27898795 .. 27901762
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0199600.1

Sequence Viewer

Length: 795 bp
ATGAAATGTGGAAATGCTAAGACTCCAACTCCACTGCCACCCTCAAGCATAGATGGAGTTGAATTTGATTGGAAATTTGCATTTGCGGGATTTGGAAGTGGGTTGTTTGTGGGAGTGGTACTTGTGAACATCACCATCACTCAGAAGCGTGAGTTGTTTCTGGAGATAGTTGGAATGTTGACCAGGCTAATCAAAAGGATCAAAAGGGAGAGAATGCGAACGAAGTCGTTCTCTGTCTCAGTGAAGATGAACCGGCAAGTTTCACTTCAGGAAATAAAACGTAATGGAATGTCTAAGCATTTAGAGACCACAGTGATTGCATTAGTTATTGCAGGTGTAATAAGAAGTCCAGAAACCTCCTCTTCTGAATATGCTACTTCTCCTTCTGGAACTAATTATGATGTGTTCTTGAGTTTCAAAGGCATGGACACTCGCAAGACTTTTACAGATCACCTCTATACAGCCCTTGTCAACGCTGGACTCCGTACTTCGGGAGATGACCCTGAACTTGAGAAGGGAGAAAGTTTCAAGGAAAATCTAGAGAAAGCACTCCATCGAAGTTCTGTTATTGTGCTTTCAAAAGAGTGTGCATCTTTCAGGTGGTGCCTTCATGAGCTTGTGACAATCCTTGAACGCAAGAGGACCTCCTCCGGTCAACATGTTCTACCAGTCTTCGACGACATCGATCCATCTCATGTGAGGAAGCAGGCAGAAAATCTTGCAAAGATTCCTACATACCAAGACAATCAGTGTTTGGAGAAGTTAAATGACTGGCGGGCAGCTAATAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

264

Amino Acids

29.7

Weight (kDa)

9.31

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 132 - 261 3e-27 TIR domain
TIR_2 PF13676 135 - 230 7.4e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 603
AciI CCGC 2 cut(s) 86, 775
AclWI GGATC 2 cut(s) 206, 680
AcsI RAATTY 2 cut(s) 62, 74
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 2 cut(s) 120, 487
AfiI CCNNNNNNNGG 1 cut(s) 490
AflIII ACRYGT 1 cut(s) 658
AgsI TTSAA 5 cut(s) 62, 418, 529, 579, 632
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 2 cut(s) 616, 782
AluI AGCT 2 cut(s) 616, 782
Alw26I GTCTC 2 cut(s) 241, 299
AlwI GGATC 2 cut(s) 206, 680
ApeKI GCWGC 1 cut(s) 779
ApoI RAATTY 2 cut(s) 62, 74
Asp700I GAANNNNTTC 1 cut(s) 227
AspS9I GGNCC 1 cut(s) 642
AsuHPI GGTGA 2 cut(s) 124, 443
AvaII GGWCC 1 cut(s) 642
BanI GGYRCC 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 664
BbvI GCAGC 1 cut(s) 791
BccI CCATC 4 cut(s) 47, 143, 561, 697
BciT130I CCWGG 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 241, 299
BfaI CTAG 2 cut(s) 539, 793
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 1 cut(s) 780
BlsI GCNGC 1 cut(s) 781
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 1 cut(s) 605
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 664
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 3 cut(s) 28, 430, 530
Bsa29I ATCGAT 1 cut(s) 684
BsaI GGTCTC 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 2 cut(s) 668, 776
BseBI CCWGG 1 cut(s) 184
BseCI ATCGAT 1 cut(s) 684
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 155, 252
BseNI ACTGG 2 cut(s) 668, 776
BseRI GAGGAG 2 cut(s) 349, 637
BseXI GCAGC 1 cut(s) 791
BshNI GGYRCC 1 cut(s) 603
BshVI ATCGAT 1 cut(s) 684
BsiSI CCGG 2 cut(s) 253, 651
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 2 cut(s) 241, 299
BsmI GAATGC 1 cut(s) 219
Bso31I GGTCTC 1 cut(s) 299
Bsp143I GATC 3 cut(s) 198, 448, 685
BspACI CCGC 2 cut(s) 86, 775
BspCNI CTCAG 2 cut(s) 154, 251
BspDI ATCGAT 1 cut(s) 684
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 605
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 206, 680
BspT107I GGYRCC 1 cut(s) 603
BspTNI GGTCTC 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 252
BsrI ACTGG 2 cut(s) 668, 776
BssAI RCCGGY 1 cut(s) 252
BssMI GATC 3 cut(s) 198, 448, 685
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 708, 777
BstDEI CTNAG 4 cut(s) 18, 141, 238, 294
BstKTI GATC 3 cut(s) 201, 451, 688
BstMAI GTCTC 2 cut(s) 241, 299
BstMBI GATC 3 cut(s) 198, 448, 685
BstNI CCWGG 1 cut(s) 184
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 791
BstV2I GAAGAC 1 cut(s) 664
Bsu15I ATCGAT 1 cut(s) 684
BsuTUI ATCGAT 1 cut(s) 684
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 4 cut(s) 32, 246, 318, 755
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 708, 777
CciI TCATGA 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 642
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 2 cut(s) 119, 486
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 4 cut(s) 424, 611, 659, 695
CviJI RGCY 4 cut(s) 187, 464, 616, 782
CviKI_1 RGCY 4 cut(s) 187, 464, 616, 782
CviQI GTAC 2 cut(s) 119, 486
DdeI CTNAG 4 cut(s) 18, 141, 238, 294
DpnI GATC 3 cut(s) 200, 450, 687
DpnII GATC 3 cut(s) 198, 448, 685
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 642
Eco57I CTGAAG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 182
FaeI CATG 4 cut(s) 427, 614, 662, 698
FaiI YATR 9 cut(s) 50, 372, 399, 425, 459, 612, 660, 696, 736
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 4 cut(s) 423, 610, 658, 694
FauI CCCGC 2 cut(s) 79, 768
Fnu4HI GCNGC 1 cut(s) 780
Fsp4HI GCNGC 1 cut(s) 780
FspBI CTAG 2 cut(s) 539, 793
GluI GCNGC 1 cut(s) 780
GsuI CTGGAG 1 cut(s) 182
HapII CCGG 2 cut(s) 253, 651
Hin1II CATG 4 cut(s) 427, 614, 662, 698
HincII GTYRAC 3 cut(s) 180, 472, 656
HindII GTYRAC 3 cut(s) 180, 472, 656
HinfI GANTC 3 cut(s) 22, 480, 727
HpaII CCGG 2 cut(s) 253, 651
HphI GGTGA 2 cut(s) 124, 443
Hpy166II GTNNAC 4 cut(s) 127, 180, 472, 656
Hpy188I TCNGA 2 cut(s) 144, 367
Hpy188III TCNNGA 8 cut(s) 161, 269, 350, 387, 409, 492, 539, 611
Hpy8I GTNNAC 4 cut(s) 127, 180, 472, 656
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 3 cut(s) 393, 508, 617
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 5 cut(s) 80, 320, 332, 590, 722
HpyF3I CTNAG 4 cut(s) 18, 141, 238, 294
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 4 cut(s) 427, 614, 662, 698
Kzo9I GATC 3 cut(s) 198, 448, 685
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 1 cut(s) 599
MaeI CTAG 2 cut(s) 539, 793
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 1 cut(s) 619
MalI GATC 3 cut(s) 200, 450, 687
MboI GATC 3 cut(s) 198, 448, 685
MboII GAAGA 3 cut(s) 256, 354, 664
MluCI AATT 3 cut(s) 62, 74, 394
MlyI GAGTC 2 cut(s) 16, 474
MmeI TCCRAC 2 cut(s) 50, 151
MnlI CCTC 8 cut(s) 52, 367, 370, 464, 633, 655, 658, 693
MroXI GAANNNNTTC 1 cut(s) 227
MseI TTAA 1 cut(s) 764
MspI CCGG 2 cut(s) 253, 651
MspR9I CCNGG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 184
NdeII GATC 3 cut(s) 198, 448, 685
NlaIII CATG 4 cut(s) 427, 614, 662, 698
NlaIV GGNNCC 1 cut(s) 605
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 1 cut(s) 662
PagI TCATGA 1 cut(s) 610
PaqCI CACCTGC 1 cut(s) 323
PciI ACATGT 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 681
PctI GAATGC 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 727
PkrI GCNGC 1 cut(s) 781
PleI GAGTC 2 cut(s) 16, 474
PpsI GAGTC 2 cut(s) 16, 474
PpuMI RGGWCCY 1 cut(s) 642
PscI ACATGT 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 1 cut(s) 605
PspPI GGNCC 1 cut(s) 642
PspPPI RGGWCCY 1 cut(s) 642
RsaI GTAC 2 cut(s) 120, 487
RsaNI GTAC 2 cut(s) 119, 486
SaqAI TTAA 1 cut(s) 764
SatI GCNGC 1 cut(s) 780
Sau3AI GATC 3 cut(s) 198, 448, 685
Sau96I GGNCC 1 cut(s) 642
SchI GAGTC 2 cut(s) 16, 474
ScrFI CCNGG 1 cut(s) 184
SetI ASST 8 cut(s) 283, 337, 359, 456, 602, 618, 647, 784
SfaNI GCATC 1 cut(s) 599
SinI GGWCC 1 cut(s) 642
SmlI CTYRAG 3 cut(s) 43, 409, 509
SmoI CTYRAG 3 cut(s) 43, 409, 509
Sse9I AATT 3 cut(s) 62, 74, 394
SsiI CCGC 2 cut(s) 86, 775
SspMI CTAG 2 cut(s) 539, 793
StyD4I CCNGG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 313
TaiI ACGT 1 cut(s) 283
TaqI TCGA 3 cut(s) 556, 675, 684
TasI AATT 3 cut(s) 62, 74, 394
TfiI GAWTC 1 cut(s) 727
Tru1I TTAA 1 cut(s) 764
Tru9I TTAA 1 cut(s) 764
TscAI CASTG 4 cut(s) 39, 246, 318, 755
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 1 cut(s) 779
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 3 cut(s) 17, 263, 599
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 4 cut(s) 39, 246, 318, 755
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 2 cut(s) 62, 74
XbaI TCTAGA 1 cut(s) 538
XceI RCATGY 1 cut(s) 662
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 2 cut(s) 539, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.