Rw1G018470

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016704
Physical Location & Seq
Forward (+)
40783413 .. 40785659
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018470.1

Sequence Viewer

Length: 846 bp
ATGTGTCCAGGGCCACAACCAAATTTTAACGACAAACGTTCGTCGGCTAAACACAAGGATGGGAATAAACGATTTCAAACTATAAAGCCTATCCTTCTTCACTTGGGGTCCTCAGAAAATGTCTCGTCACCCACTCGGAGCTGCTCTCCCGGCGCACACCGAAATCCTCATTCCGATCAAAAACGCCGCCGTTCTGAACCTCAAAACTCCCCCACCTCTCCTCAACTCTCTCTCACCCAAACCCGTAAACCCTGCCCTGCAATAAGGGCACTCTCCATCGATGAAATTCCCCCAAATGCCCTCCGCCGGAAGCGCGACTCTAACTGGAGAGGAGGCTTTAGCGTAGGGGTAGACTTGGGATTGTCCCGCACCGGCCTCGCCCTCAGCAAAGGCTTCTCCGTCCGTCCCCTCACCGTGTTGAATTTGAGAGGGCAGAAGCTTGAGGTTAAAATCCTTGAGATTGCAAAGCAAGAGGAGGCTGATGAGTTTATAATCGGGCTTCCGAAATCGAGTGATGGGAAAGAGACACCTCAGTCTAACAAAGTTCGTAGTGTCGCCGGGAGACTTGCTGCTACAGCTGCTGAGAGGGGTTGGAGAGTATACCTGCAGGATGAAAATGGGACATCCATCGAAGCCATGGATCGAATGATTAACATGGGCCTCAGCAGGTCTGATCGTCAAAGCAAAATCGATGCCTACGCTGCCATGATGGTGCTGGAGCGATATTTTTCCATGTCAGGTGAAGGAACTGAGCTTGTACTGCCCAAGAATTTGGATCTGCAAGACAGACTTCGAAGAGGTCCACCCAAAGATGTTGATTACTTCTCTGAAGACGATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

281

Amino Acids

31.28

Weight (kDa)

9.53

Isoelectric Point (pI)

67.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 115 - 244 7.1e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 491
AasI GACNNNNNNGTC 1 cut(s) 532
Acc36I ACCTGC 2 cut(s) 612, 657
AccI GTMKAC 2 cut(s) 351, 600
AccII CGCG 1 cut(s) 315
AciI CCGC 3 cut(s) 187, 304, 367
AclI AACGTT 1 cut(s) 37
AclWI GGATC 2 cut(s) 648, 783
AcsI RAATTY 4 cut(s) 22, 285, 421, 769
AfaI GTAC 1 cut(s) 759
AfiI CCNNNNNNNGG 1 cut(s) 306
AgsI TTSAA 2 cut(s) 77, 421
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 4 cut(s) 141, 439, 578, 754
AluI AGCT 4 cut(s) 141, 439, 578, 754
Alw26I GTCTC 3 cut(s) 127, 518, 556
AlwI GGATC 2 cut(s) 648, 783
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 3 cut(s) 11, 373, 658
ApeKI GCWGC 4 cut(s) 141, 569, 578, 701
ApoI RAATTY 4 cut(s) 22, 285, 421, 769
AspLEI GCGC 2 cut(s) 155, 315
AspS9I GGNCC 4 cut(s) 11, 108, 658, 800
AsuC2I CCSGG 2 cut(s) 150, 559
AsuHPI GGTGA 4 cut(s) 120, 226, 403, 752
AsuII TTCGAA 1 cut(s) 793
AvaII GGWCC 2 cut(s) 108, 800
BaeGI GKGCMC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 837
BbvCI CCTCAGC 2 cut(s) 383, 662
BbvI GCAGC 4 cut(s) 128, 556, 565, 688
BccI CCATC 5 cut(s) 53, 284, 509, 635, 703
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 1 cut(s) 9
BcnI CCSGG 2 cut(s) 150, 559
BcoDI GTCTC 3 cut(s) 127, 518, 556
BfmI CTRYAG 2 cut(s) 573, 605
BfuAI ACCTGC 2 cut(s) 612, 657
BisI GCNGC 5 cut(s) 142, 187, 570, 579, 702
BlsI GCNGC 5 cut(s) 143, 188, 571, 580, 703
Bme1390I CCNGG 3 cut(s) 9, 150, 559
Bme18I GGWCC 2 cut(s) 108, 800
BmgT120I GGNCC 4 cut(s) 11, 108, 658, 800
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 3 cut(s) 9, 150, 559
BmsI GCATC 1 cut(s) 682
BpiI GAAGAC 1 cut(s) 837
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpmI CTGGAG 2 cut(s) 346, 737
Bpu10I CCTNAGC 2 cut(s) 383, 662
Bpu14I TTCGAA 1 cut(s) 793
BpuEI CTTGAG 2 cut(s) 461, 476
BpuMI CCSGG 2 cut(s) 150, 559
Bsa29I ATCGAT 2 cut(s) 279, 690
BsaJI CCNNGG 2 cut(s) 8, 636
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 329
BseBI CCWGG 1 cut(s) 9
BseCI ATCGAT 2 cut(s) 279, 690
BseDI CCNNGG 2 cut(s) 8, 636
BseGI GGATG 3 cut(s) 64, 616, 623
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMII CTCAG 6 cut(s) 126, 397, 545, 573, 676, 741
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 3 cut(s) 210, 345, 488
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 4 cut(s) 128, 556, 565, 688
Bsh1236I CGCG 1 cut(s) 315
BshFI GGCC 3 cut(s) 13, 375, 660
BshVI ATCGAT 2 cut(s) 279, 690
BsiSI CCGG 4 cut(s) 150, 307, 372, 558
BslFI GGGAC 3 cut(s) 349, 390, 634
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 3 cut(s) 127, 518, 556
BsmFI GGGAC 3 cut(s) 349, 390, 634
BsnI GGCC 3 cut(s) 13, 375, 660
Bsp119I TTCGAA 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 4 cut(s) 175, 640, 673, 775
Bsp19I CCATGG 1 cut(s) 636
BspACI CCGC 3 cut(s) 187, 304, 367
BspANI GGCC 3 cut(s) 13, 375, 660
BspCNI CTCAG 6 cut(s) 125, 396, 544, 574, 675, 742
BspDI ATCGAT 2 cut(s) 279, 690
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 609
BspMI ACCTGC 2 cut(s) 612, 657
BspPI GGATC 2 cut(s) 648, 783
BspT104I TTCGAA 1 cut(s) 793
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 8, 636
BssMI GATC 4 cut(s) 175, 640, 673, 775
BssNAI GTATAC 1 cut(s) 601
BssT1I CCWWGG 1 cut(s) 636
Bst1107I GTATAC 1 cut(s) 601
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 790
BstBI TTCGAA 1 cut(s) 793
BstDEI CTNAG 6 cut(s) 112, 383, 531, 582, 662, 750
BstDSI CCRYGG 1 cut(s) 636
BstF5I GGATG 3 cut(s) 64, 616, 623
BstFNI CGCG 1 cut(s) 315
BstHHI GCGC 2 cut(s) 155, 315
BstKTI GATC 4 cut(s) 178, 643, 676, 778
BstMAI GTCTC 3 cut(s) 127, 518, 556
BstMBI GATC 4 cut(s) 175, 640, 673, 775
BstMWI GCNNNNNNNGC 7 cut(s) 150, 266, 312, 575, 578, 701, 760
BstNI CCWGG 1 cut(s) 9
BstSCI CCNGG 3 cut(s) 7, 148, 557
BstSFI CTRYAG 2 cut(s) 573, 605
BstSLI GKGCMC 1 cut(s) 271
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 4 cut(s) 128, 556, 565, 688
BstV2I GAAGAC 1 cut(s) 837
BstX2I RGATCY 1 cut(s) 775
BstXI CCANNNNNNTGG 1 cut(s) 772
BstYI RGATCY 1 cut(s) 775
BstZ17I GTATAC 1 cut(s) 601
Bsu15I ATCGAT 2 cut(s) 279, 690
BsuRI GGCC 3 cut(s) 13, 375, 660
BsuTUI ATCGAT 2 cut(s) 279, 690
BtgI CCRYGG 1 cut(s) 636
BtsCI GGATG 3 cut(s) 64, 616, 623
BveI ACCTGC 2 cut(s) 612, 657
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 2 cut(s) 155, 315
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 4 cut(s) 11, 108, 658, 800
ClaI ATCGAT 2 cut(s) 279, 690
Csp6I GTAC 1 cut(s) 758
CviAII CATG 4 cut(s) 637, 655, 706, 733
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 6 cut(s) 112, 383, 531, 582, 662, 750
DpnI GATC 4 cut(s) 177, 642, 675, 777
DpnII GATC 4 cut(s) 175, 640, 673, 775
DrdI GACNNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
EciI GGCGGA 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 108, 800
EcoO109I RGGNCCY 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 4 cut(s) 640, 658, 709, 736
FaiI YATR 7 cut(s) 83, 491, 601, 638, 656, 707, 734
FalI AAGNNNNNCTT 2 cut(s) 774, 806
FaqI GGGAC 3 cut(s) 349, 390, 634
FatI CATG 4 cut(s) 636, 654, 705, 732
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 2 cut(s) 351, 600
Fnu4HI GCNGC 5 cut(s) 142, 187, 570, 579, 702
FokI GGATG 3 cut(s) 71, 610, 623
Fsp4HI GCNGC 5 cut(s) 142, 187, 570, 579, 702
GlaI GCGC 2 cut(s) 154, 314
GluI GCNGC 5 cut(s) 142, 187, 570, 579, 702
GsuI CTGGAG 2 cut(s) 346, 737
HaeIII GGCC 3 cut(s) 13, 375, 660
HapII CCGG 4 cut(s) 150, 307, 372, 558
HhaI GCGC 2 cut(s) 155, 315
Hin1II CATG 4 cut(s) 640, 658, 709, 736
Hin6I GCGC 2 cut(s) 153, 313
HinP1I GCGC 2 cut(s) 153, 313
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 1 cut(s) 317
HpaII CCGG 4 cut(s) 150, 307, 372, 558
HphI GGTGA 4 cut(s) 120, 226, 403, 752
Hpy166II GTNNAC 4 cut(s) 248, 352, 601, 803
Hpy188I TCNGA 7 cut(s) 115, 138, 175, 196, 504, 673, 829
Hpy8I GTNNAC 4 cut(s) 248, 352, 601, 803
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 104, 737
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 4 cut(s) 260, 464, 607, 781
HpyF10VI GCNNNNNNNGC 7 cut(s) 150, 266, 312, 575, 578, 701, 760
HpyF3I CTNAG 6 cut(s) 112, 383, 531, 582, 662, 750
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 4 cut(s) 640, 658, 709, 736
HspAI GCGC 2 cut(s) 153, 313
Kzo9I GATC 4 cut(s) 175, 640, 673, 775
LmnI GCTCC 2 cut(s) 138, 718
Lsp1109I GCAGC 4 cut(s) 128, 556, 565, 688
LweI GCATC 1 cut(s) 682
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 126
MalI GATC 4 cut(s) 177, 642, 675, 777
MboI GATC 4 cut(s) 175, 640, 673, 775
MboII GAAGA 3 cut(s) 89, 807, 842
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 4 cut(s) 22, 285, 421, 769
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 572
MseI TTAA 3 cut(s) 27, 447, 651
MslI CAYNNNNRTG 2 cut(s) 57, 710
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 4 cut(s) 150, 307, 372, 558
MspR9I CCNGG 3 cut(s) 9, 150, 559
MvaI CCWGG 1 cut(s) 9
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 7 cut(s) 150, 266, 312, 575, 578, 701, 760
NciI CCSGG 2 cut(s) 150, 559
NcoI CCATGG 1 cut(s) 636
NdeII GATC 4 cut(s) 175, 640, 673, 775
NlaIII CATG 4 cut(s) 640, 658, 709, 736
NlaIV GGNNCC 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 126
NspV TTCGAA 1 cut(s) 793
PkrI GCNGC 5 cut(s) 143, 188, 571, 580, 703
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 108
PsiI TTATAA 1 cut(s) 491
Psp1406I AACGTT 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 4 cut(s) 11, 108, 658, 800
PspPPI RGGWCCY 1 cut(s) 108
PstI CTGCAG 1 cut(s) 609
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 775
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 2 cut(s) 57, 710
SaqAI TTAA 3 cut(s) 27, 447, 651
SatI GCNGC 5 cut(s) 142, 187, 570, 579, 702
Sau3AI GATC 4 cut(s) 175, 640, 673, 775
Sau96I GGNCC 4 cut(s) 11, 108, 658, 800
SbfI CCTGCAGG 1 cut(s) 609
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 3 cut(s) 9, 150, 559
SdaI CCTGCAGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 1 cut(s) 682
SfcI CTRYAG 2 cut(s) 573, 605
SfuI TTCGAA 1 cut(s) 793
SinI GGWCC 2 cut(s) 108, 800
SmiMI CAYNNNNRTG 2 cut(s) 57, 710
SmlI CTYRAG 2 cut(s) 440, 455
SmoI CTYRAG 2 cut(s) 440, 455
Sse8387I CCTGCAGG 1 cut(s) 609
Sse9I AATT 4 cut(s) 22, 285, 421, 769
SsiI CCGC 3 cut(s) 187, 304, 367
StyD4I CCNGG 3 cut(s) 7, 148, 557
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 1 cut(s) 40
TaqI TCGA 6 cut(s) 279, 509, 630, 643, 690, 793
TasI AATT 4 cut(s) 22, 285, 421, 769
TatI WGTACW 1 cut(s) 757
TauI GCSGC 1 cut(s) 189
Tru1I TTAA 3 cut(s) 27, 447, 651
Tru9I TTAA 3 cut(s) 27, 447, 651
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 4 cut(s) 141, 569, 578, 701
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 2 cut(s) 297, 627
TspGWI ACGGA 2 cut(s) 388, 392
VpaK11BI GGWCC 2 cut(s) 108, 800
XapI RAATTY 4 cut(s) 22, 285, 421, 769
XcmI CCANNNNNNNNNTGG 2 cut(s) 634, 712
XmiI GTMKAC 2 cut(s) 351, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.