RLG00000028610

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016704
Physical Location & Seq
Reverse (-)
25016467 .. 25018463
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028610

Sequence Viewer

Length: 729 bp
ATGTCTCGTCACCCACTCAAAGCTTCTCTCCCGGCGCAAACCGGACTCCTCATTCCGATCAAAAACGCCGCCGTTCTGAACATCAAAACCGCCCCCACCTCTCCTCAACTCTCTCTCACCCAAACCCGTAAACCCTGCCCTGCAATAAGGGCAGTCTCCGTCGATGAATTTCCCCCAAATGCCCTCCGCAGGAAGCGCGACTCCAACTGGAGAGGAGGCTTTAGCGTAGGGGTAGACTTGGGATTGTCCCGCACCGGCCTCGCCCTCAGCAAAGGCTTCTCCGTCCGTCCCCTCACCGTGTTGAATTTGAGAGGGCAGAAGCTTGAGGTTAAAATCCTTGAGATTGCAAAGCAAGAGGAGGCTGATGAGTTTATAATCGGGCTTCCGAAATCGAGTGATGGGAAAGAGACACCTCAGTCTAACAAAGTTCGTAGTGTCGCCGGGAGACTTGCTGCTACTGCTGCTGAGAGGGGTTGGAGAGTATACCTGCAGGATGAAAATGGGACATCCATCGAAGCCATGGATCGAATGATTAACATGGGCCTCAGCAGGTCTGATCGTCAAAGCAAAATCGATGCCTACGCTGCCATGATGGTGCTGGAGCGATATTTTTCCATGTCAGGTGAAGGAACTGAGCTTGTACTGCCCAAGAATTTGGATCTGCAAGACAGACTTCGAAGAGGTCCACCCAAAGACATTGATTACTTCTCTGAAGACGATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

243

Amino Acids

26.74

Weight (kDa)

9.32

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 76 - 205 4.9e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AasI GACNNNNNNGTC 1 cut(s) 415
Acc36I ACCTGC 2 cut(s) 495, 540
AccI GTMKAC 2 cut(s) 234, 483
AccII CGCG 1 cut(s) 198
AciI CCGC 4 cut(s) 69, 90, 187, 250
AclWI GGATC 2 cut(s) 531, 666
AcsI RAATTY 3 cut(s) 167, 304, 652
AfaI GTAC 1 cut(s) 642
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 1 cut(s) 304
AluBI AGCT 3 cut(s) 23, 322, 637
AluI AGCT 3 cut(s) 23, 322, 637
Alw26I GTCTC 4 cut(s) 9, 160, 401, 439
AlwI GGATC 2 cut(s) 531, 666
AoxI GGCC 2 cut(s) 256, 541
ApeKI GCWGC 3 cut(s) 452, 461, 584
ApoI RAATTY 3 cut(s) 167, 304, 652
AspLEI GCGC 2 cut(s) 37, 198
AspS9I GGNCC 2 cut(s) 541, 683
AsuC2I CCSGG 2 cut(s) 32, 442
AsuHPI GGTGA 3 cut(s) 109, 286, 635
AsuII TTCGAA 1 cut(s) 676
AvaII GGWCC 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 720
BbvCI CCTCAGC 2 cut(s) 266, 545
BbvI GCAGC 3 cut(s) 439, 448, 571
BccI CCATC 3 cut(s) 392, 518, 586
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BcnI CCSGG 2 cut(s) 32, 442
BcoDI GTCTC 4 cut(s) 9, 160, 401, 439
BfmI CTRYAG 1 cut(s) 488
BfuAI ACCTGC 2 cut(s) 495, 540
BisI GCNGC 4 cut(s) 69, 453, 462, 585
BlsI GCNGC 4 cut(s) 70, 454, 463, 586
Bme1390I CCNGG 2 cut(s) 32, 442
Bme18I GGWCC 1 cut(s) 683
BmgT120I GGNCC 2 cut(s) 541, 683
BmrFI CCNGG 2 cut(s) 32, 442
BmsI GCATC 1 cut(s) 565
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 229, 620
Bpu10I CCTNAGC 2 cut(s) 266, 545
Bpu14I TTCGAA 1 cut(s) 676
BpuEI CTTGAG 2 cut(s) 344, 359
BpuMI CCSGG 2 cut(s) 32, 442
Bsa29I ATCGAT 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 212
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 2 cut(s) 499, 506
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 5 cut(s) 280, 428, 456, 559, 624
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 4 cut(s) 38, 93, 228, 371
BseXI GCAGC 3 cut(s) 439, 448, 571
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 258, 543
BshVI ATCGAT 1 cut(s) 573
BsiSI CCGG 4 cut(s) 32, 42, 255, 441
BslFI GGGAC 3 cut(s) 232, 273, 517
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 4 cut(s) 9, 160, 401, 439
BsmFI GGGAC 3 cut(s) 232, 273, 517
BsnI GGCC 2 cut(s) 258, 543
Bsp119I TTCGAA 1 cut(s) 676
Bsp143I GATC 4 cut(s) 57, 523, 556, 658
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 4 cut(s) 69, 90, 187, 250
BspANI GGCC 2 cut(s) 258, 543
BspCNI CTCAG 5 cut(s) 279, 427, 457, 558, 625
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 1 cut(s) 198
BspMAI CTGCAG 1 cut(s) 492
BspMI ACCTGC 2 cut(s) 495, 540
BspPI GGATC 2 cut(s) 531, 666
BspT104I TTCGAA 1 cut(s) 676
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 4 cut(s) 57, 523, 556, 658
BssNAI GTATAC 1 cut(s) 484
BssT1I CCWWGG 1 cut(s) 519
Bst1107I GTATAC 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 1 cut(s) 673
BstBI TTCGAA 1 cut(s) 676
BstDEI CTNAG 5 cut(s) 266, 414, 465, 545, 633
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 2 cut(s) 499, 506
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 2 cut(s) 37, 198
BstKTI GATC 4 cut(s) 60, 526, 559, 661
BstMAI GTCTC 4 cut(s) 9, 160, 401, 439
BstMBI GATC 4 cut(s) 57, 523, 556, 658
BstMWI GCNNNNNNNGC 6 cut(s) 149, 195, 458, 461, 584, 643
BstSCI CCNGG 2 cut(s) 30, 440
BstSFI CTRYAG 1 cut(s) 488
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 439, 448, 571
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 658
BstXI CCANNNNNNTGG 1 cut(s) 655
BstYI RGATCY 1 cut(s) 658
BstZ17I GTATAC 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 2 cut(s) 258, 543
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 2 cut(s) 499, 506
BveI ACCTGC 2 cut(s) 495, 540
CfoI GCGC 2 cut(s) 37, 198
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 2 cut(s) 541, 683
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 1 cut(s) 641
CviAII CATG 4 cut(s) 520, 538, 589, 616
CviQI GTAC 1 cut(s) 641
DdeI CTNAG 5 cut(s) 266, 414, 465, 545, 633
DpnI GATC 4 cut(s) 59, 525, 558, 660
DpnII GATC 4 cut(s) 57, 523, 556, 658
DrdI GACNNNNNNGTC 1 cut(s) 415
DseDI GACNNNNNNGTC 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 673
Eco130I CCWWGG 1 cut(s) 519
Eco47I GGWCC 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 4 cut(s) 523, 541, 592, 619
FaiI YATR 6 cut(s) 374, 484, 521, 539, 590, 617
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 3 cut(s) 232, 273, 517
FatI CATG 4 cut(s) 519, 537, 588, 615
FauI CCCGC 1 cut(s) 257
FblI GTMKAC 2 cut(s) 234, 483
Fnu4HI GCNGC 4 cut(s) 69, 453, 462, 585
FokI GGATG 2 cut(s) 493, 506
Fsp4HI GCNGC 4 cut(s) 69, 453, 462, 585
GlaI GCGC 2 cut(s) 36, 197
GluI GCNGC 4 cut(s) 69, 453, 462, 585
GsuI CTGGAG 2 cut(s) 229, 620
HaeIII GGCC 2 cut(s) 258, 543
HapII CCGG 4 cut(s) 32, 42, 255, 441
HhaI GCGC 2 cut(s) 37, 198
Hin1II CATG 4 cut(s) 523, 541, 592, 619
Hin6I GCGC 2 cut(s) 35, 196
HinP1I GCGC 2 cut(s) 35, 196
HindIII AAGCTT 2 cut(s) 21, 320
HinfI GANTC 2 cut(s) 45, 200
HpaII CCGG 4 cut(s) 32, 42, 255, 441
HphI GGTGA 3 cut(s) 109, 286, 635
Hpy166II GTNNAC 4 cut(s) 131, 235, 484, 686
Hpy188I TCNGA 5 cut(s) 57, 78, 387, 556, 712
Hpy8I GTNNAC 4 cut(s) 131, 235, 484, 686
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 620
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4V TGCA 4 cut(s) 143, 347, 490, 664
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 195, 458, 461, 584, 643
HpyF3I CTNAG 5 cut(s) 266, 414, 465, 545, 633
Hsp92II CATG 4 cut(s) 523, 541, 592, 619
HspAI GCGC 2 cut(s) 35, 196
Kzo9I GATC 4 cut(s) 57, 523, 556, 658
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 3 cut(s) 439, 448, 571
LweI GCATC 1 cut(s) 565
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 59, 525, 558, 660
MboI GATC 4 cut(s) 57, 523, 556, 658
MboII GAAGA 2 cut(s) 690, 725
MflI RGATCY 1 cut(s) 658
MluCI AATT 3 cut(s) 167, 304, 652
MlyI GAGTC 2 cut(s) 39, 194
MmeI TCCRAC 2 cut(s) 228, 455
MseI TTAA 2 cut(s) 330, 534
MslI CAYNNNNRTG 1 cut(s) 593
MspI CCGG 4 cut(s) 32, 42, 255, 441
MspR9I CCNGG 2 cut(s) 32, 442
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 6 cut(s) 149, 195, 458, 461, 584, 643
NciI CCSGG 2 cut(s) 32, 442
NcoI CCATGG 1 cut(s) 519
NdeII GATC 4 cut(s) 57, 523, 556, 658
NlaIII CATG 4 cut(s) 523, 541, 592, 619
NmuCI GTSAC 1 cut(s) 8
NspV TTCGAA 1 cut(s) 676
PkrI GCNGC 4 cut(s) 70, 454, 463, 586
PleI GAGTC 2 cut(s) 39, 194
PpsI GAGTC 2 cut(s) 39, 194
PsiI TTATAA 1 cut(s) 374
PspPI GGNCC 2 cut(s) 541, 683
PstI CTGCAG 1 cut(s) 492
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 1 cut(s) 642
RsaNI GTAC 1 cut(s) 641
RseI CAYNNNNRTG 1 cut(s) 593
SaqAI TTAA 2 cut(s) 330, 534
SatI GCNGC 4 cut(s) 69, 453, 462, 585
Sau3AI GATC 4 cut(s) 57, 523, 556, 658
Sau96I GGNCC 2 cut(s) 541, 683
SbfI CCTGCAGG 1 cut(s) 492
SchI GAGTC 2 cut(s) 39, 194
ScrFI CCNGG 2 cut(s) 32, 442
SdaI CCTGCAGG 1 cut(s) 492
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 488
SfuI TTCGAA 1 cut(s) 676
SinI GGWCC 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 2 cut(s) 323, 338
SmoI CTYRAG 2 cut(s) 323, 338
Sse8387I CCTGCAGG 1 cut(s) 492
Sse9I AATT 3 cut(s) 167, 304, 652
SsiI CCGC 4 cut(s) 69, 90, 187, 250
StyD4I CCNGG 2 cut(s) 30, 440
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 298
TaqI TCGA 6 cut(s) 162, 392, 513, 526, 573, 676
TasI AATT 3 cut(s) 167, 304, 652
TatI WGTACW 1 cut(s) 640
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 330, 534
Tru9I TTAA 2 cut(s) 330, 534
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 3 cut(s) 452, 461, 584
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 2 cut(s) 180, 510
TspGWI ACGGA 3 cut(s) 148, 271, 275
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 3 cut(s) 167, 304, 652
XcmI CCANNNNNNNNNTGG 2 cut(s) 517, 595
XmiI GTMKAC 2 cut(s) 234, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.