MD07G1114500.v1.1

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016704
Physical Location & Seq
Forward (+)
13728597 .. 13731114
2518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1114500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTTTTGTCATCGAGTAGGAGCTCCGCCGTCACAAACCGAACTCCTTCCGCTCCGGAGACGTACCGTTTCTGAAGTCCAAAATGTCCCCGCGTCCCTTCAACTCTCTCTCACCCAAAACCGTAAAAAGGGTCCTCGTATAAGGGCGGTCTCCATAGATGAAATTCCACCAAATGCCCTCCGCCGGAAGCGAGACCCGCAATGGAGAGGCGGGTTTAGCGTAGGGGTAGATTTGGGACTGTCACGCACCGGCCTTGCCGTCAGCAAAGGCTTCACCGTCCGTCCTCTCACCGTATTGAATTTGAGAGGGCAGAAGCTTGAGGATCAGCTGCTTGAGATCGCAAAGAAGCAGGAGGCAGATGAGTTCATAGTTGGCCTTCCGAAATCGAGTGATGGGAAAGAGACGCCTCAGTCTAACAAAGTTCGTAGCGTTGTTGGAAGGCTTGCTGTTAGTGCCGCTCAGAGGGGTTGGAGAGTGTACCTTCAAGATGAAAATGGGACGTCCATGGAAGCCATGGATCGGATGATTAACGTGGGCCTCGACAAGTCTGATCGACAAAGCCAAATCGATGCCTACGCTGCCATGATGGTGCTGGAGAGATATTTTTCCATGTCAGGTGAGGGCACGGAGCTCGTACTGCCCAGGAATCTGGATCTTCAAGACAAACTTCGAAGAGGGCTGCCTATAGATACCGATTTTTTCGATGAAGATTACGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

241

Amino Acids

26.94

Weight (kDa)

9.01

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 74 - 203 5.6e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AatII GACGTC 1 cut(s) 503
AccBSI CCGCTC 2 cut(s) 52, 458
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 8 cut(s) 26, 50, 90, 146, 181, 197, 210, 456
AclWI GGATC 3 cut(s) 330, 525, 660
AcsI RAATTY 2 cut(s) 162, 298
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 2 cut(s) 404, 500
AfaI GTAC 3 cut(s) 64, 479, 636
AfiI CCNNNNNNNGG 4 cut(s) 127, 183, 462, 519
AgsI TTSAA 4 cut(s) 101, 298, 485, 659
AjnI CCWGG 1 cut(s) 641
AluBI AGCT 4 cut(s) 23, 316, 328, 631
AluI AGCT 4 cut(s) 23, 316, 328, 631
Alw21I GWGCWC 2 cut(s) 25, 633
Alw26I GTCTC 4 cut(s) 52, 154, 186, 395
AlwI GGATC 3 cut(s) 330, 525, 660
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 3 cut(s) 250, 373, 535
ApeKI GCWGC 3 cut(s) 328, 578, 679
ApoI RAATTY 2 cut(s) 162, 298
Asp700I GAANNNNTTC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 131, 535
AsuHPI GGTGA 4 cut(s) 103, 265, 280, 629
AsuII TTCGAA 1 cut(s) 670
AvaII GGWCC 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 25, 633
Bbv12I GWGCWC 2 cut(s) 25, 633
BbvI GCAGC 3 cut(s) 315, 565, 666
BccI CCATC 2 cut(s) 386, 580
BceAI ACGGC 2 cut(s) 13, 242
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BciT130I CCWGG 1 cut(s) 643
BcoDI GTCTC 4 cut(s) 52, 154, 186, 395
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 4 cut(s) 329, 456, 579, 680
BlsI GCNGC 4 cut(s) 330, 457, 580, 681
Bme1390I CCNGG 1 cut(s) 643
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 131, 535
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 559
BpmI CTGGAG 1 cut(s) 614
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 338, 353
Bsa29I ATCGAT 1 cut(s) 567
BsaHI GRCGYC 2 cut(s) 404, 500
BsaI GGTCTC 2 cut(s) 154, 186
BsaJI CCNNGG 3 cut(s) 504, 513, 641
BsaWI WCCGGW 1 cut(s) 54
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 183, 462, 519
Bse118I RCCGGY 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 206
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 1 cut(s) 643
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 3 cut(s) 504, 513, 641
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 4 cut(s) 127, 183, 462, 519
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 2 cut(s) 422, 473
BseSI GKGCMC 1 cut(s) 626
BseXI GCAGC 3 cut(s) 315, 565, 666
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 252, 375, 537
BshVI ATCGAT 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 25, 633
BsiSI CCGG 3 cut(s) 55, 184, 249
BslFI GGGAC 4 cut(s) 71, 79, 249, 511
BslI CCNNNNNNNGG 4 cut(s) 127, 183, 462, 519
BsmAI GTCTC 4 cut(s) 52, 154, 186, 395
BsmBI CGTCTC 2 cut(s) 52, 395
BsmFI GGGAC 4 cut(s) 71, 79, 249, 511
BsnI GGCC 3 cut(s) 252, 375, 537
Bso31I GGTCTC 2 cut(s) 154, 186
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 3 cut(s) 25, 626, 633
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 5 cut(s) 322, 336, 517, 550, 652
Bsp19I CCATGG 2 cut(s) 504, 513
BspACI CCGC 8 cut(s) 26, 50, 90, 146, 181, 197, 210, 456
BspANI GGCC 3 cut(s) 252, 375, 537
BspCNI CTCAG 2 cut(s) 421, 472
BspDI ATCGAT 1 cut(s) 567
BspEI TCCGGA 1 cut(s) 54
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 132
BspPI GGATC 3 cut(s) 330, 525, 660
BspT104I TTCGAA 1 cut(s) 670
BspTNI GGTCTC 2 cut(s) 154, 186
BsrBI CCGCTC 2 cut(s) 52, 458
BsrDI GCAATG 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 504, 513, 641
BssMI GATC 5 cut(s) 322, 336, 517, 550, 652
BssNI GRCGYC 2 cut(s) 404, 500
BssT1I CCWWGG 2 cut(s) 504, 513
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 5 cut(s) 67, 122, 240, 277, 292
Bst6I CTCTTC 1 cut(s) 667
BstACI GRCGYC 2 cut(s) 404, 500
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 2 cut(s) 408, 459
BstDSI CCRYGG 2 cut(s) 504, 513
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 1 cut(s) 92
BstKTI GATC 5 cut(s) 325, 339, 520, 553, 655
BstMAI GTCTC 4 cut(s) 52, 154, 186, 395
BstMBI GATC 5 cut(s) 322, 336, 517, 550, 652
BstMWI GCNNNNNNNGC 5 cut(s) 196, 216, 452, 578, 637
BstNI CCWGG 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 641
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 626
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 3 cut(s) 315, 565, 666
BstX2I RGATCY 1 cut(s) 652
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 652
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 3 cut(s) 252, 375, 537
BsuTUI ATCGAT 1 cut(s) 567
BtgI CCRYGG 2 cut(s) 504, 513
BtsCI GGATG 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 131, 535
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 2 cut(s) 81, 412
Csp6I GTAC 3 cut(s) 63, 478, 635
CviAII CATG 4 cut(s) 505, 514, 583, 610
CviQI GTAC 3 cut(s) 63, 478, 635
DdeI CTNAG 2 cut(s) 408, 459
DpnI GATC 5 cut(s) 324, 338, 519, 552, 654
DpnII GATC 5 cut(s) 322, 336, 517, 550, 652
DrdI GACNNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 667
EciI GGCGGA 2 cut(s) 15, 170
Ecl136II GAGCTC 2 cut(s) 23, 631
Eco130I CCWWGG 2 cut(s) 504, 513
Eco24I GRGCYC 2 cut(s) 25, 633
Eco31I GGTCTC 2 cut(s) 154, 186
Eco47I GGWCC 1 cut(s) 131
Eco53kI GAGCTC 2 cut(s) 23, 631
Eco57I CTGAAG 1 cut(s) 93
EcoICRI GAGCTC 2 cut(s) 23, 631
EcoO109I RGGNCCY 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 504, 513
EcoT38I GRGCYC 2 cut(s) 25, 633
ErhI CCWWGG 2 cut(s) 504, 513
Esp3I CGTCTC 2 cut(s) 52, 395
FaeI CATG 4 cut(s) 508, 517, 586, 613
FaiI YATR 8 cut(s) 140, 155, 368, 506, 515, 584, 611, 686
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 4 cut(s) 71, 79, 249, 511
FatI CATG 4 cut(s) 504, 513, 582, 609
FauI CCCGC 3 cut(s) 97, 203, 204
Fnu4HI GCNGC 4 cut(s) 329, 456, 579, 680
FokI GGATG 1 cut(s) 535
FriOI GRGCYC 2 cut(s) 25, 633
Fsp4HI GCNGC 4 cut(s) 329, 456, 579, 680
GluI GCNGC 4 cut(s) 329, 456, 579, 680
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 3 cut(s) 252, 375, 537
HapII CCGG 3 cut(s) 55, 184, 249
HgaI GACGC 2 cut(s) 81, 412
Hin1I GRCGYC 2 cut(s) 404, 500
Hin1II CATG 4 cut(s) 508, 517, 586, 613
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 1 cut(s) 646
HpaII CCGG 3 cut(s) 55, 184, 249
HphI GGTGA 4 cut(s) 103, 265, 280, 629
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 5 cut(s) 73, 381, 462, 522, 550
Hpy188III TCNNGA 4 cut(s) 55, 485, 650, 659
Hpy8I GTNNAC 1 cut(s) 478
HpyAV CCTTC 5 cut(s) 56, 107, 386, 432, 491
HpyCH4III ACNGT 5 cut(s) 67, 122, 240, 277, 292
HpyCH4IV ACGT 3 cut(s) 61, 500, 531
HpyF10VI GCNNNNNNNGC 5 cut(s) 196, 216, 452, 578, 637
HpyF3I CTNAG 2 cut(s) 408, 459
HpySE526I ACGT 3 cut(s) 61, 500, 531
Hsp92I GRCGYC 2 cut(s) 404, 500
Hsp92II CATG 4 cut(s) 508, 517, 586, 613
Kpn2I TCCGGA 1 cut(s) 54
Kzo9I GATC 5 cut(s) 322, 336, 517, 550, 652
LmnI GCTCC 4 cut(s) 20, 28, 57, 628
LpnPI CCDG 9 cut(s) 68, 197, 262, 335, 578, 600, 628, 635, 655
Lsp1109I GCAGC 3 cut(s) 315, 565, 666
LweI GCATC 1 cut(s) 559
MaeII ACGT 3 cut(s) 61, 500, 531
MaeIII GTNAC 2 cut(s) 30, 240
MalI GATC 5 cut(s) 324, 338, 519, 552, 654
MbiI CCGCTC 2 cut(s) 52, 458
MboI GATC 5 cut(s) 322, 336, 517, 550, 652
MboII GAAGA 3 cut(s) 647, 684, 719
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 3 cut(s) 25, 626, 633
MluCI AATT 2 cut(s) 162, 298
MmeI TCCRAC 2 cut(s) 415, 449
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 3 cut(s) 55, 184, 249
MspR9I CCNGG 1 cut(s) 643
MvaI CCWGG 1 cut(s) 643
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 5 cut(s) 196, 216, 452, 578, 637
NcoI CCATGG 2 cut(s) 504, 513
NdeII GATC 5 cut(s) 322, 336, 517, 550, 652
NlaIII CATG 4 cut(s) 508, 517, 586, 613
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 30, 240
NspV TTCGAA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 646
PkrI GCNGC 4 cut(s) 330, 457, 580, 681
PpuMI RGGWCCY 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 25, 633
Psp5II RGGWCCY 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 641
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 131, 535
PspPPI RGGWCCY 1 cut(s) 131
PsuI RGATCY 1 cut(s) 652
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 3 cut(s) 64, 479, 636
RsaNI GTAC 3 cut(s) 63, 478, 635
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 2 cut(s) 25, 633
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 4 cut(s) 329, 456, 579, 680
Sau3AI GATC 5 cut(s) 322, 336, 517, 550, 652
Sau96I GGNCC 2 cut(s) 131, 535
ScrFI CCNGG 1 cut(s) 643
SduI GDGCHC 3 cut(s) 25, 626, 633
SetI ASST 9 cut(s) 25, 64, 318, 330, 483, 503, 534, 619, 633
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 317, 332
SmoI CTYRAG 2 cut(s) 317, 332
Sse9I AATT 2 cut(s) 162, 298
SsiI CCGC 8 cut(s) 26, 50, 90, 146, 181, 197, 210, 456
SstI GAGCTC 2 cut(s) 25, 633
StyD4I CCNGG 1 cut(s) 641
StyI CCWWGG 2 cut(s) 504, 513
TaaI ACNGT 5 cut(s) 67, 122, 240, 277, 292
TaiI ACGT 3 cut(s) 64, 503, 534
TaqI TCGA 7 cut(s) 13, 386, 540, 553, 567, 670, 702
TasI AATT 2 cut(s) 162, 298
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 1 cut(s) 646
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TseFI GTSAC 2 cut(s) 30, 240
TseI GCWGC 3 cut(s) 328, 578, 679
Tsp45I GTSAC 2 cut(s) 30, 240
TspDTI ATGAA 4 cut(s) 174, 355, 504, 720
TspGWI ACGGA 2 cut(s) 269, 641
VpaK11BI GGWCC 1 cut(s) 131
XapI RAATTY 2 cut(s) 162, 298
XcmI CCANNNNNNNNNTGG 2 cut(s) 511, 589
XmnI GAANNNNTTC 1 cut(s) 45
ZraI GACGTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.