Rh1CG201600

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0016704
Physical Location & Seq
Forward (+)
43803989 .. 43806043
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG201600.1

Sequence Viewer

Length: 789 bp
ATGGGAATAAACGATTTCAAACTACAAAGCCTATCCCACCTCACTTGGGGTCCTCAGAAAATGTCTCGTCACCCACTCGGAGCTGCTCTCCCGGCGCACACCGAACTCCTCATTCCGATCAAAAACGCCGCCGTTCTGAACCTCAAAACCGCCCCCACCTCTCCTCAACTCTCTCTCACCCAAACCCGTAAACCCTGCCCTGCAATAAGGGCAGTCTCCATCGATGAAATTCCCCCAAATGCCCTCCGCCGGAAGCGCGACTCCAACTGGAGAGGAGGCTTTAGCGTAGGGGTAGACTTGGGATTGTCCCGCACCGGCCTCGCCCTCAGCAAAGGCTTCTCCGTCCGTCCCCTCACCGTGTTGAATTTGAGAGGGCAGAAGCTTGAGGGTAAAATCCTTGAGATTGCAAAGCAAGAGGAGGCAGATGAGTTTATAATCGGGCTTCCGAAATCGAGTGATGGGAAAGAGACACCTCAGTCTAACAAAGTTCGTAGTGTCGCCGGGAGACTTGCTGCTACTGCTGCTGAGAGGGGTTGGAGAGTATACCTGCAGGATGAAAATGGGACATCCATCGAAGCCATGGATCGAATGATTAACATGGGCCTCAGCAGGTCTGATCGTCAAAGCAAAATCGATGCCTACGCTGCCATGATGGTGCTGGAGCGATATTTTTCCATGTCAGGTGAAGGAACTGAGCTTGTACTGCCCAAGAATTTGGATCTGCAAGACAGACTTCGAAGAGGTCCACCCAAAGATGTTGATTACTTCTCTGAAGACGATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

262

Amino Acids

28.94

Weight (kDa)

9.14

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 96 - 225 5.6e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AasI GACNNNNNNGTC 1 cut(s) 475
Acc36I ACCTGC 2 cut(s) 555, 600
AccI GTMKAC 2 cut(s) 294, 543
AccII CGCG 1 cut(s) 258
AciI CCGC 4 cut(s) 129, 150, 247, 310
AclWI GGATC 2 cut(s) 591, 726
AcsI RAATTY 3 cut(s) 228, 364, 712
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 46, 249
AgsI TTSAA 2 cut(s) 19, 364
AluBI AGCT 3 cut(s) 83, 382, 697
AluI AGCT 3 cut(s) 83, 382, 697
Alw26I GTCTC 4 cut(s) 69, 220, 461, 499
AlwI GGATC 2 cut(s) 591, 726
AoxI GGCC 2 cut(s) 316, 601
ApeKI GCWGC 4 cut(s) 83, 512, 521, 644
ApoI RAATTY 3 cut(s) 228, 364, 712
AspLEI GCGC 2 cut(s) 97, 258
AspS9I GGNCC 3 cut(s) 50, 601, 743
AsuC2I CCSGG 2 cut(s) 92, 502
AsuHPI GGTGA 4 cut(s) 62, 169, 346, 695
AsuII TTCGAA 1 cut(s) 736
AvaII GGWCC 2 cut(s) 50, 743
BbsI GAAGAC 1 cut(s) 780
BbvCI CCTCAGC 2 cut(s) 326, 605
BbvI GCAGC 4 cut(s) 70, 499, 508, 631
BccI CCATC 4 cut(s) 227, 452, 578, 646
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BcnI CCSGG 2 cut(s) 92, 502
BcoDI GTCTC 4 cut(s) 69, 220, 461, 499
BfmI CTRYAG 1 cut(s) 548
BfuAI ACCTGC 2 cut(s) 555, 600
BisI GCNGC 5 cut(s) 84, 129, 513, 522, 645
BlsI GCNGC 5 cut(s) 85, 130, 514, 523, 646
Bme1390I CCNGG 2 cut(s) 92, 502
Bme18I GGWCC 2 cut(s) 50, 743
BmgT120I GGNCC 3 cut(s) 50, 601, 743
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 2 cut(s) 92, 502
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 780
BplI GAGNNNNNCTC 2 cut(s) 72, 104
BpmI CTGGAG 2 cut(s) 289, 680
Bpu10I CCTNAGC 2 cut(s) 326, 605
Bpu14I TTCGAA 1 cut(s) 736
BpuEI CTTGAG 2 cut(s) 404, 419
BpuMI CCSGG 2 cut(s) 92, 502
Bsa29I ATCGAT 2 cut(s) 222, 633
BsaJI CCNNGG 1 cut(s) 579
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 249
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 272
BseCI ATCGAT 2 cut(s) 222, 633
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 2 cut(s) 559, 566
BseLI CCNNNNNNNGG 2 cut(s) 46, 249
BseMII CTCAG 6 cut(s) 68, 340, 488, 516, 619, 684
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 4 cut(s) 98, 153, 288, 431
BseXI GCAGC 4 cut(s) 70, 499, 508, 631
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 2 cut(s) 318, 603
BshVI ATCGAT 2 cut(s) 222, 633
BsiSI CCGG 4 cut(s) 92, 250, 315, 501
BslFI GGGAC 3 cut(s) 292, 333, 577
BslI CCNNNNNNNGG 2 cut(s) 46, 249
BsmAI GTCTC 4 cut(s) 69, 220, 461, 499
BsmFI GGGAC 3 cut(s) 292, 333, 577
BsnI GGCC 2 cut(s) 318, 603
Bsp119I TTCGAA 1 cut(s) 736
Bsp143I GATC 4 cut(s) 117, 583, 616, 718
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 4 cut(s) 129, 150, 247, 310
BspANI GGCC 2 cut(s) 318, 603
BspCNI CTCAG 6 cut(s) 67, 339, 487, 517, 618, 685
BspDI ATCGAT 2 cut(s) 222, 633
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 552
BspMI ACCTGC 2 cut(s) 555, 600
BspPI GGATC 2 cut(s) 591, 726
BspT104I TTCGAA 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 4 cut(s) 117, 583, 616, 718
BssNAI GTATAC 1 cut(s) 544
BssT1I CCWWGG 1 cut(s) 579
Bst1107I GTATAC 1 cut(s) 544
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 736
BstDEI CTNAG 6 cut(s) 54, 326, 474, 525, 605, 693
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 559, 566
BstFNI CGCG 1 cut(s) 258
BstHHI GCGC 2 cut(s) 97, 258
BstKTI GATC 4 cut(s) 120, 586, 619, 721
BstMAI GTCTC 4 cut(s) 69, 220, 461, 499
BstMBI GATC 4 cut(s) 117, 583, 616, 718
BstMWI GCNNNNNNNGC 7 cut(s) 92, 209, 255, 518, 521, 644, 703
BstSCI CCNGG 2 cut(s) 90, 500
BstSFI CTRYAG 1 cut(s) 548
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 4 cut(s) 70, 499, 508, 631
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 715
BstYI RGATCY 1 cut(s) 718
BstZ17I GTATAC 1 cut(s) 544
Bsu15I ATCGAT 2 cut(s) 222, 633
BsuRI GGCC 2 cut(s) 318, 603
BsuTUI ATCGAT 2 cut(s) 222, 633
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 2 cut(s) 559, 566
BveI ACCTGC 2 cut(s) 555, 600
CfoI GCGC 2 cut(s) 97, 258
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 50, 601, 743
ClaI ATCGAT 2 cut(s) 222, 633
Csp6I GTAC 1 cut(s) 701
CspCI CAANNNNNGTGG 2 cut(s) 26, 61
CviAII CATG 4 cut(s) 580, 598, 649, 676
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 6 cut(s) 54, 326, 474, 525, 605, 693
DpnI GATC 4 cut(s) 119, 585, 618, 720
DpnII GATC 4 cut(s) 117, 583, 616, 718
DrdI GACNNNNNNGTC 1 cut(s) 475
DseDI GACNNNNNNGTC 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 733
EarI CTCTTC 1 cut(s) 733
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 50, 743
EcoO109I RGGNCCY 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 4 cut(s) 583, 601, 652, 679
FaiI YATR 6 cut(s) 434, 544, 581, 599, 650, 677
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 3 cut(s) 292, 333, 577
FatI CATG 4 cut(s) 579, 597, 648, 675
FauI CCCGC 1 cut(s) 317
FblI GTMKAC 2 cut(s) 294, 543
Fnu4HI GCNGC 5 cut(s) 84, 129, 513, 522, 645
FokI GGATG 2 cut(s) 553, 566
Fsp4HI GCNGC 5 cut(s) 84, 129, 513, 522, 645
GlaI GCGC 2 cut(s) 96, 257
GluI GCNGC 5 cut(s) 84, 129, 513, 522, 645
GsuI CTGGAG 2 cut(s) 289, 680
HaeIII GGCC 2 cut(s) 318, 603
HapII CCGG 4 cut(s) 92, 250, 315, 501
HhaI GCGC 2 cut(s) 97, 258
Hin1II CATG 4 cut(s) 583, 601, 652, 679
Hin6I GCGC 2 cut(s) 95, 256
HinP1I GCGC 2 cut(s) 95, 256
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 260
HpaII CCGG 4 cut(s) 92, 250, 315, 501
HphI GGTGA 4 cut(s) 62, 169, 346, 695
Hpy166II GTNNAC 4 cut(s) 191, 295, 544, 746
Hpy188I TCNGA 7 cut(s) 57, 80, 117, 138, 447, 616, 772
Hpy8I GTNNAC 4 cut(s) 191, 295, 544, 746
HpyAV CCTTC 1 cut(s) 680
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 4 cut(s) 203, 407, 550, 724
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 209, 255, 518, 521, 644, 703
HpyF3I CTNAG 6 cut(s) 54, 326, 474, 525, 605, 693
Hsp92II CATG 4 cut(s) 583, 601, 652, 679
HspAI GCGC 2 cut(s) 95, 256
Kzo9I GATC 4 cut(s) 117, 583, 616, 718
LmnI GCTCC 2 cut(s) 80, 661
Lsp1109I GCAGC 4 cut(s) 70, 499, 508, 631
LweI GCATC 1 cut(s) 625
MaeIII GTNAC 1 cut(s) 68
MalI GATC 4 cut(s) 119, 585, 618, 720
MboI GATC 4 cut(s) 117, 583, 616, 718
MboII GAAGA 2 cut(s) 750, 785
MflI RGATCY 1 cut(s) 718
MluCI AATT 3 cut(s) 228, 364, 712
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 2 cut(s) 288, 515
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 1 cut(s) 653
MspI CCGG 4 cut(s) 92, 250, 315, 501
MspR9I CCNGG 2 cut(s) 92, 502
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 7 cut(s) 92, 209, 255, 518, 521, 644, 703
NciI CCSGG 2 cut(s) 92, 502
NcoI CCATGG 1 cut(s) 579
NdeII GATC 4 cut(s) 117, 583, 616, 718
NlaIII CATG 4 cut(s) 583, 601, 652, 679
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 68
NspV TTCGAA 1 cut(s) 736
PkrI GCNGC 5 cut(s) 85, 130, 514, 523, 646
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 50
PsiI TTATAA 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 3 cut(s) 50, 601, 743
PspPPI RGGWCCY 1 cut(s) 50
PstI CTGCAG 1 cut(s) 552
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 1 cut(s) 653
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 5 cut(s) 84, 129, 513, 522, 645
Sau3AI GATC 4 cut(s) 117, 583, 616, 718
Sau96I GGNCC 3 cut(s) 50, 601, 743
SbfI CCTGCAGG 1 cut(s) 552
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 92, 502
SdaI CCTGCAGG 1 cut(s) 552
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 736
SinI GGWCC 2 cut(s) 50, 743
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 2 cut(s) 383, 398
SmoI CTYRAG 2 cut(s) 383, 398
Sse8387I CCTGCAGG 1 cut(s) 552
Sse9I AATT 3 cut(s) 228, 364, 712
SsiI CCGC 4 cut(s) 129, 150, 247, 310
StyD4I CCNGG 2 cut(s) 90, 500
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 1 cut(s) 358
TaqI TCGA 6 cut(s) 222, 452, 573, 586, 633, 736
TasI AATT 3 cut(s) 228, 364, 712
TatI WGTACW 1 cut(s) 700
TauI GCSGC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 4 cut(s) 83, 512, 521, 644
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 2 cut(s) 240, 570
TspGWI ACGGA 2 cut(s) 331, 335
VpaK11BI GGWCC 2 cut(s) 50, 743
XapI RAATTY 3 cut(s) 228, 364, 712
XcmI CCANNNNNNNNNTGG 2 cut(s) 577, 655
XmiI GTMKAC 2 cut(s) 294, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.