AT1G12320

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013984
Physical Location & Seq
Forward (+)
4186624 .. 4190342
3719 bp
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UTR
Exon/CDS
Intron
AT1G12320.1

Sequence Viewer

Length: 639 bp
ATGAAATTGGTTTGGTCTCCGGAAACAGCATCAAAGGCTTATATAGACACCGTTAAATCGTGTGAGAATCTTGAAACGCCGGACGCGGCGGAGCTAATAGCGGCGATGGCGGCGGGATGGAACGTGAAGCTGATCGTGGAAACTTGGTCAGAAGGAGACGCAATAGCTTCAAGTATTGGTTTAAACGTAGCAAGCAAACACGCAAACGCTAAACACATATGTATAGTACAAAACTCAAGATCAGAATCTGCTTATCTCCAAGCTATTCAAGAATCTTCATCTCCCTTGAACTTTCCAGAGACTATCGTCGCTGAAGAACCCGGAAAAGCGATGAAGAAGCTACAAGGAGTCGACTTCTTGGTCGTGGATTGGCGGAACAAAGAATTTGCAGCGGCTGCGTTGAAAAACGCTGCGTTTGGAAACAGAGGAGCTGTTGTGGTTTGTAGAAACGGGTATTCGAGCTTGAGAAGGGTTTTAAGAGACCGGAAAGTTGTTAGAACGGTGACTCTTCCGGTCACCGGAGGTATTGAGATAGCTCATGTTGCGGCTAGGAACTCAGGGAAGAGTGGTAACAATAAGAGAAGATGGATCACACATGTTGATCAGAGATCAGGAGAGGAACATGTGTTTAGTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

212

Amino Acids

23.09

Weight (kDa)

9.17

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 1 - 211 3.3e-114 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12320 AT1G62840
fragaria_vesca FvH4_5g00680
prunus_persica Prupe.5G079600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0337631 RchiOBHm_Chr7g0201531
rosa_laevigata RLG00000003664 RLG00000033501
rosa_multiflora Rmu_sc0013887.1_g000001
rosa_roxburghii Rroxscaffold_3G00255240
rosa_rugosa Rorug07G0064700
rosa_samantha Rh6AG501400 Rh7AG191300 Rh7BG193600 Rh7CG202500 Rh7DG196500
rosa_wichuraiana Rw7G016770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 86
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 8 cut(s) 86, 89, 101, 110, 113, 373, 392, 545
AclWI GGATC 1 cut(s) 596
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 518
AflIII ACRYGT 2 cut(s) 595, 622
AgsI TTSAA 5 cut(s) 74, 171, 269, 289, 403
AluBI AGCT 8 cut(s) 94, 130, 167, 263, 340, 431, 462, 536
AluI AGCT 8 cut(s) 94, 130, 167, 263, 340, 431, 462, 536
Alw26I GTCTC 4 cut(s) 21, 150, 293, 474
AlwI GGATC 1 cut(s) 596
AlwNI CAGNNNCTG 2 cut(s) 248, 395
Aor13HI TCCGGA 1 cut(s) 19
ApeKI GCWGC 3 cut(s) 389, 395, 410
ApoI RAATTY 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 508, 514
BbvI GCAGC 3 cut(s) 382, 397, 401
BccI CCATC 3 cut(s) 100, 111, 579
BclI TGATCA 1 cut(s) 601
BcnI CCSGG 1 cut(s) 321
BcoDI GTCTC 4 cut(s) 21, 150, 293, 474
BfaI CTAG 2 cut(s) 549, 637
BisI GCNGC 8 cut(s) 87, 102, 111, 390, 393, 396, 411, 546
BlsI GCNGC 8 cut(s) 88, 103, 112, 391, 394, 397, 412, 547
Bme1390I CCNGG 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 38
BoxI GACNNNNGTC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 220, 484
BpuMI CCSGG 1 cut(s) 321
BsaBI GATNNNNATC 1 cut(s) 244
BsaI GGTCTC 2 cut(s) 21, 474
BsaWI WCCGGW 4 cut(s) 19, 483, 511, 518
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 244
BseAI TCCGGA 1 cut(s) 19
BseGI GGATG 1 cut(s) 122
BseJI GATNNNNATC 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 518
BseMII CTCAG 1 cut(s) 570
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 3 cut(s) 382, 397, 401
Bsh1236I CGCG 1 cut(s) 86
BsiSI CCGG 6 cut(s) 20, 80, 321, 484, 512, 519
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 4 cut(s) 21, 150, 293, 474
BsmBI CGTCTC 1 cut(s) 150
Bso31I GGTCTC 2 cut(s) 21, 474
Bsp13I TCCGGA 1 cut(s) 19
Bsp143I GATC 5 cut(s) 132, 239, 588, 601, 608
BspACI CCGC 8 cut(s) 86, 89, 101, 110, 113, 373, 392, 545
BspCNI CTCAG 1 cut(s) 569
BspEI TCCGGA 1 cut(s) 19
BspFNI CGCG 1 cut(s) 86
BspPI GGATC 1 cut(s) 596
BspTNI GGTCTC 2 cut(s) 21, 474
BssMI GATC 5 cut(s) 132, 239, 588, 601, 608
Bst4CI ACNGT 2 cut(s) 52, 502
Bst6I CTCTTC 2 cut(s) 513, 557
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 1 cut(s) 556
BstEII GGTNACC 1 cut(s) 514
BstF5I GGATG 1 cut(s) 122
BstFNI CGCG 1 cut(s) 86
BstKTI GATC 5 cut(s) 135, 242, 591, 604, 611
BstMAI GTCTC 4 cut(s) 21, 150, 293, 474
BstMBI GATC 5 cut(s) 132, 239, 588, 601, 608
BstMWI GCNNNNNNNGC 5 cut(s) 35, 107, 110, 395, 542
BstNSI RCATGY 2 cut(s) 599, 626
BstPAI GACNNNNGTC 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 319
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 3 cut(s) 382, 397, 401
BtgZI GCGATG 2 cut(s) 119, 344
BtsCI GGATG 1 cut(s) 122
Cac8I GCNNGC 1 cut(s) 193
CaiI CAGNNNCTG 2 cut(s) 248, 395
CseI GACGC 2 cut(s) 92, 167
Csp6I GTAC 1 cut(s) 227
CviAII CATG 3 cut(s) 539, 596, 623
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 1 cut(s) 556
DpnI GATC 5 cut(s) 134, 241, 590, 603, 610
DpnII GATC 5 cut(s) 132, 239, 588, 601, 608
DraI TTTAAA 1 cut(s) 183
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
Eam1104I CTCTTC 2 cut(s) 513, 557
EarI CTCTTC 2 cut(s) 513, 557
EciI GGCGGA 2 cut(s) 104, 388
Eco31I GGTCTC 2 cut(s) 21, 474
Eco57I CTGAAG 1 cut(s) 333
Eco91I GGTNACC 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 3 cut(s) 542, 599, 626
FaiI YATR 8 cut(s) 42, 44, 218, 220, 224, 540, 597, 624
FatI CATG 3 cut(s) 538, 595, 622
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 218
FbaI TGATCA 1 cut(s) 601
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 8 cut(s) 87, 102, 111, 390, 393, 396, 411, 546
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 8 cut(s) 87, 102, 111, 390, 393, 396, 411, 546
FspBI CTAG 2 cut(s) 549, 637
GluI GCNGC 8 cut(s) 87, 102, 111, 390, 393, 396, 411, 546
HapII CCGG 6 cut(s) 20, 80, 321, 484, 512, 519
HgaI GACGC 2 cut(s) 92, 167
Hin1II CATG 3 cut(s) 542, 599, 626
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 5 cut(s) 67, 245, 272, 348, 505
HpaII CCGG 6 cut(s) 20, 80, 321, 484, 512, 519
HphI GGTGA 2 cut(s) 508, 514
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 3 cut(s) 151, 244, 606
Hpy188III TCNNGA 6 cut(s) 20, 71, 237, 269, 296, 612
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 146, 462
HpyCH4III ACNGT 2 cut(s) 52, 502
HpyCH4IV ACGT 2 cut(s) 123, 186
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 107, 110, 395, 542
HpyF3I CTNAG 1 cut(s) 556
HpySE526I ACGT 2 cut(s) 123, 186
Hsp92II CATG 3 cut(s) 542, 599, 626
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 5 cut(s) 132, 239, 588, 601, 608
LmnI GCTCC 2 cut(s) 91, 428
LpnPI CCDG 9 cut(s) 33, 93, 309, 334, 497, 525, 532, 543, 597
Lsp1109I GCAGC 3 cut(s) 382, 397, 401
LweI GCATC 1 cut(s) 38
MaeI CTAG 2 cut(s) 549, 637
MaeII ACGT 2 cut(s) 123, 186
MaeIII GTNAC 3 cut(s) 502, 514, 569
MalI GATC 5 cut(s) 134, 241, 590, 603, 610
MboI GATC 5 cut(s) 132, 239, 588, 601, 608
MboII GAAGA 6 cut(s) 267, 326, 346, 500, 574, 594
MluCI AATT 2 cut(s) 5, 383
MlyI GAGTC 2 cut(s) 357, 499
MnlI CCTC 3 cut(s) 419, 515, 610
MroI TCCGGA 1 cut(s) 19
MseI TTAA 3 cut(s) 54, 182, 476
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 6 cut(s) 20, 80, 321, 484, 512, 519
MspR9I CCNGG 1 cut(s) 321
MssI GTTTAAAC 1 cut(s) 183
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 5 cut(s) 35, 107, 110, 395, 542
NciI CCSGG 1 cut(s) 321
NdeI CATATG 1 cut(s) 218
NdeII GATC 5 cut(s) 132, 239, 588, 601, 608
NlaIII CATG 3 cut(s) 542, 599, 626
NmuCI GTSAC 2 cut(s) 502, 514
NspI RCATGY 2 cut(s) 599, 626
PciI ACATGT 2 cut(s) 595, 622
PfeI GAWTC 3 cut(s) 67, 245, 272
PkrI GCNGC 8 cut(s) 88, 103, 112, 391, 394, 397, 412, 547
PleI GAGTC 2 cut(s) 356, 499
PmeI GTTTAAAC 1 cut(s) 183
PpsI GAGTC 2 cut(s) 356, 499
PscI ACATGT 2 cut(s) 595, 622
PshAI GACNNNNGTC 1 cut(s) 305
PspEI GGTNACC 1 cut(s) 514
PstNI CAGNNNCTG 2 cut(s) 248, 395
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 3 cut(s) 54, 182, 476
SatI GCNGC 8 cut(s) 87, 102, 111, 390, 393, 396, 411, 546
Sau3AI GATC 5 cut(s) 132, 239, 588, 601, 608
SchI GAGTC 2 cut(s) 357, 499
ScrFI CCNGG 1 cut(s) 321
SfaNI GCATC 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 235, 463
SmoI CTYRAG 2 cut(s) 235, 463
Sse9I AATT 2 cut(s) 5, 383
SsiI CCGC 8 cut(s) 86, 89, 101, 110, 113, 373, 392, 545
SspMI CTAG 2 cut(s) 549, 637
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 52, 502
TaiI ACGT 2 cut(s) 126, 189
TaqI TCGA 2 cut(s) 351, 458
TasI AATT 2 cut(s) 5, 383
TatI WGTACW 1 cut(s) 226
TauI GCSGC 5 cut(s) 89, 104, 113, 395, 548
TfiI GAWTC 3 cut(s) 67, 245, 272
Tru1I TTAA 3 cut(s) 54, 182, 476
Tru9I TTAA 3 cut(s) 54, 182, 476
TseFI GTSAC 2 cut(s) 502, 514
TseI GCWGC 3 cut(s) 389, 395, 410
Tsp45I GTSAC 2 cut(s) 502, 514
TspDTI ATGAA 3 cut(s) 17, 267, 347
XapI RAATTY 1 cut(s) 383
XceI RCATGY 2 cut(s) 599, 626
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 549, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.