AT1G14450

NADH dehydrogenase ubiquinone 1 beta subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016686
Physical Location & Seq
Reverse (-)
4946192 .. 4947670
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14450.2

Sequence Viewer

Length: 222 bp
ATGGCGAAGCCTTTGGGAACAACCGGCGAATTTTTCCGGCGAAGAGATGAATGGAGGAAGCATCCGATGCTTTCGAATCAAATGAGACACGCTCTTCCCGGACTTGGAATCGGCGTCGCCGCCTTCTGCGTTTATCTCGTCGGTGAGCAGATCTACAACAAAGCTTTGGCTCCTTCCAAATCATCTCACCACCACCAGGAACAGACTGCTCCGTCTCACTGA

Protein Analysis

73

Amino Acids

8.22

Weight (kDa)

9.74

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUF_B12 PF08122 38 - 89 3.7e-09 NADH-ubiquinone oxidoreductase B12 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14450 AT1G14450 AT2G02510
fragaria_vesca FvH4_5g27520
malus_domestica MD08G1249300.v1.1 MD15G1444100.v1.1
prunus_persica Prupe.1G586500_v2.0.a1 Prupe.1G586500_v2.0.a1
pyrus_communis pycom08g21580
rosa_chinensis RchiOBHm_Chr7g0218891
rosa_roxburghii Rroxscaffold_3G00240420
rosa_rugosa Rorug07G0179500
rosa_wichuraiana Rw7G027330 Rw7G028460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AciI CCGC 1 cut(s) 120
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 104, 196
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 1 cut(s) 164
AluI AGCT 1 cut(s) 164
Alw26I GTCTC 1 cut(s) 79
ApoI RAATTY 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 155, 179
AsuII TTCGAA 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 197
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 79
BglII AGATCT 1 cut(s) 150
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 99, 197
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 2 cut(s) 99, 197
BmsI GCATC 2 cut(s) 57, 70
BplI GAGNNNNNCTC 2 cut(s) 76, 108
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 196
Bse118I RCCGGY 1 cut(s) 23
BseBI CCWGG 1 cut(s) 197
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 104, 196
BsiSI CCGG 3 cut(s) 24, 37, 99
BslI CCNNNNNNNGG 2 cut(s) 104, 196
BsmAI GTCTC 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 74
Bsp143I GATC 1 cut(s) 150
BspACI CCGC 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 171
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
BssMI GATC 1 cut(s) 150
BssNI GRCGYC 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 197
Bst6I CTCTTC 2 cut(s) 37, 99
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 74
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 1 cut(s) 153
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 97, 195
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BtsCI GGATG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 23
CseI GACGC 1 cut(s) 103
CviJI RGCY 3 cut(s) 10, 164, 170
CviKI_1 RGCY 3 cut(s) 10, 164, 170
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 37, 99
EarI CTCTTC 2 cut(s) 37, 99
EcoRII CCWGG 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 120
HapII CCGG 3 cut(s) 24, 37, 99
HgaI GACGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 2 cut(s) 76, 108
HpaII CCGG 3 cut(s) 24, 37, 99
HphI GGTGA 2 cut(s) 155, 179
Hpy188I TCNGA 1 cut(s) 66
Hpy99I CGWCG 2 cut(s) 119, 143
HpyAV CCTTC 2 cut(s) 133, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
Hsp92I GRCGYC 1 cut(s) 114
Kzo9I GATC 1 cut(s) 150
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 2 cut(s) 175, 214
LpnPI CCDG 5 cut(s) 37, 50, 112, 182, 209
LweI GCATC 2 cut(s) 57, 70
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MboII GAAGA 2 cut(s) 54, 86
MflI RGATCY 1 cut(s) 150
MluCI AATT 1 cut(s) 29
MnlI CCTC 1 cut(s) 48
MspI CCGG 3 cut(s) 24, 37, 99
MspR9I CCNGG 2 cut(s) 99, 197
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 99
NdeII GATC 1 cut(s) 150
NlaIV GGNNCC 1 cut(s) 171
NspV TTCGAA 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 76, 108
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 99
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 150
ScrFI CCNGG 2 cut(s) 99, 197
SetI ASST 1 cut(s) 166
SfaNI GCATC 2 cut(s) 57, 70
SfuI TTCGAA 1 cut(s) 74
SgeI CNNG 9 cut(s) 36, 49, 101, 110, 111, 116, 149, 208, 209
Sse9I AATT 1 cut(s) 29
SsiI CCGC 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 97, 195
TaqI TCGA 1 cut(s) 74
TasI AATT 1 cut(s) 29
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 76, 108
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 201
XapI RAATTY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.