Rw7G027330

NADH dehydrogenase ubiquinone 1 beta subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016686
Physical Location & Seq
Reverse (-)
35256461 .. 35258358
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G027330.1

Sequence Viewer

Length: 228 bp
ATGGGAAGCAGCAGCAAGCCGTTGGGACCCACAGGGGAGTTCTTCAGGAGAAGGGACGAGTGGAGGAAGCACCCGATGCTTTCGAATCAGCTCCGCCACGCCACTCCCGGCCTCGGCATCGCTCTGGTGGCCTTCGGCGTCTACTTGGTCGGCGAGCAGTTCTACAACCGCGTCATCGCTCCTCCTTCTTCTCACTCCTCCTCCTCCTCCTCTTCCTCTTCTCACTGA

Protein Analysis

75

Amino Acids

8.19

Weight (kDa)

10.4

Isoelectric Point (pI)

60.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUF_B12 PF08122 17 - 55 3.8e-08 NADH-ubiquinone oxidoreductase B12 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14450 AT1G14450 AT2G02510
fragaria_vesca FvH4_5g27520
malus_domestica MD08G1249300.v1.1 MD15G1444100.v1.1
prunus_persica Prupe.1G586500_v2.0.a1 Prupe.1G586500_v2.0.a1
pyrus_communis pycom08g21580
rosa_chinensis RchiOBHm_Chr7g0218891
rosa_roxburghii Rroxscaffold_3G00240420
rosa_rugosa Rorug07G0179500
rosa_wichuraiana Rw7G027330 Rw7G028460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 171
AciI CCGC 2 cut(s) 94, 169
AcuI CTGAAG 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 113
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
AoxI GGCC 2 cut(s) 109, 129
ApeKI GCWGC 2 cut(s) 9, 12
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 108
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 26
BbvI GCAGC 2 cut(s) 21, 24
BceAI ACGGC 1 cut(s) 4
BcnI CCSGG 1 cut(s) 108
BisI GCNGC 2 cut(s) 10, 13
BlsI GCNGC 2 cut(s) 11, 14
Bme1390I CCNGG 1 cut(s) 108
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 27, 28
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 66, 126
Bpu14I TTCGAA 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 113
BseRI GAGGAG 6 cut(s) 171, 187, 190, 193, 196, 199
BseXI GCAGC 2 cut(s) 21, 24
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 111, 131
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 2 cut(s) 39, 68
BslI CCNNNNNNNGG 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 39, 68
BsnI GGCC 2 cut(s) 111, 131
Bsp119I TTCGAA 1 cut(s) 83
BspACI CCGC 2 cut(s) 94, 169
BspANI GGCC 2 cut(s) 111, 131
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 2 cut(s) 27, 28
BspT104I TTCGAA 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 112
BssNI GRCGYC 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 217, 223
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 76
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 17, 155
BstFNI CGCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 2 cut(s) 76, 128
BstSCI CCNGG 1 cut(s) 106
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 21, 24
BsuRI GGCC 2 cut(s) 111, 131
BtgZI GCGATG 2 cut(s) 103, 160
BtsIMutI CAGTG 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 17, 155
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 2 cut(s) 127, 160
CviJI RGCY 4 cut(s) 19, 91, 111, 131
CviKI_1 RGCY 4 cut(s) 19, 91, 111, 131
Eam1104I CTCTTC 2 cut(s) 217, 223
EarI CTCTTC 2 cut(s) 217, 223
EciI GGCGGA 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 26
FaqI GGGAC 2 cut(s) 39, 68
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 10, 13
Fsp4HI GCNGC 2 cut(s) 10, 13
GluI GCNGC 2 cut(s) 10, 13
HaeIII GGCC 2 cut(s) 111, 131
HapII CCGG 1 cut(s) 108
HgaI GACGC 2 cut(s) 127, 160
Hin1I GRCGYC 1 cut(s) 138
HinfI GANTC 1 cut(s) 85
HpaII CCGG 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 142
Hpy188III TCNNGA 1 cut(s) 46
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 3 cut(s) 45, 142, 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 128
Hsp92I GRCGYC 1 cut(s) 138
KflI GGGWCCC 1 cut(s) 26
LmnI GCTCC 2 cut(s) 96, 184
LpnPI CCDG 4 cut(s) 18, 31, 110, 121
Lsp1109I GCAGC 2 cut(s) 21, 24
LweI GCATC 2 cut(s) 66, 126
MboII GAAGA 4 cut(s) 34, 180, 204, 210
MnlI CCTC 9 cut(s) 57, 122, 192, 208, 211, 214, 217, 220, 226
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 76, 128
NciI CCSGG 1 cut(s) 108
NlaIV GGNNCC 2 cut(s) 27, 28
NmeAIII GCCGAG 1 cut(s) 93
NspV TTCGAA 1 cut(s) 83
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 2 cut(s) 11, 14
PpuMI RGGWCCY 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 27, 28
PspPI GGNCC 1 cut(s) 26
PspPPI RGGWCCY 1 cut(s) 26
SatI GCNGC 2 cut(s) 10, 13
Sau96I GGNCC 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 108
SetI ASST 1 cut(s) 93
SfaNI GCATC 2 cut(s) 66, 126
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 1 cut(s) 26
SsiI CCGC 2 cut(s) 94, 169
StyD4I CCNGG 1 cut(s) 106
TaqI TCGA 1 cut(s) 83
TfiI GAWTC 1 cut(s) 85
TseI GCWGC 2 cut(s) 9, 12
VpaK11BI GGWCC 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.