AT1G15470

Contains the following InterPro domains WD40 repeat 2 (InterPro IPR019782), WD40 repeat-like-containing domain (InterPro IPR011046), WD40 repeat, conserved site (InterPro IPR019775), WD40-repeat-containing domain (InterPro IPR017986), WD40 YVTN repeat-like-containing domain (InterPro IPR015943), WD40 repeat (InterPro IPR001680), WD40 repeat, subgroup (InterPro IPR019781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009140
Physical Location & Seq
Forward (+)
5315499 .. 5317941
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15470.1

Sequence Viewer

Length: 1002 bp
ATGGGAGCTCCGTTAGTGTGCCACGGGCATTCTCGTCCCGTCGTCGATGTGGCTTATAGTCCGGTGACTCCAGATGGGTTCTTTCTCATTAGCGCCAGCAAAGATTCGAATCCGATGTTGAGGAATGGAGAGACTGGTGACTGGATTGGGACTTTTGAAGGACATAAGGGAGCAGTTTGGAGTTGCAGCCTTGATAAAAATGCTATTCGTGCTGCCTCAGCTTCTGCTGATTTCACTGCGAAAATATGGAATGCATTGACAGGAGATGAATTGCACTCCTTTGAACACAAGCACATTGTTCGTGCATGTGCCTTTTCTGAGGACACTCACCGTTTACTCACTGGTGGAATGGAGAAAATACTTCGGATATTCGATTTGAATCGGCCAGACGCACCTCCAAAAGAAGTCGGGAATTCCCCTGGTTCAATTAGAACTGTCGAATGGCTTCATAGTGATAATACAATCTTAAGCTCTTGCACAGATACCGGTGACATTAGGTTATGGGACATAAGAAGTGACAAGATTGTTCATACATTAGAAACAAAGTCCCCAGTTACTAGTGCTGAAGTAAGTCAGGATGGGCGATACATCACTACTGCTGATGGATCTAGTGTTAAGTTTTGGGACGCTAAAAATTTTGGATTGCTGAAGAGCTATGACATGCCTTGCAATGTTGAATCGGCATCGTTGGAACCGAAACACGGTAACACTTTCATTGCTGGAGGAGAAGATATGTGGGTGCACAGGTTTGATTTCCAGACTGGAGAAGAGATTGGGTGTAACAAGGGTCATCACGGGCCAGTGCACTGCGTGAGGTATGCGCCAGGAGGGGAGTCATACACCTCAGGCTCAGAAGACGGAACGGTCAGAATATGGGTGGTGGGTTCGGTAAACCATCCTGAGGAGAGCAATCTAAGCGGCCACGTGAAGCTTGTGGCAGAGGAGGTTGTGCGTAAAGCTGAAAGTCTCCGTATCAGTGAGAAAGCCGCAGAAGCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
WD40

Protein Analysis

333

Amino Acids

36.34

Weight (kDa)

5.72

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 4 - 291 2.5e-46 EIF3I
Beta-prop_WDR5 PF25175 8 - 224 1.3e-28 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 8 - 125 1.3e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 84 7.2e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 17 - 224 1.2e-21 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 22 - 95 5.5e-06 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 28 - 116 3.7e-06 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 29 - 127 6.5e-06 G protein beta WD-40 repeat protein
WD40 PF00400 49 - 84 3.9e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 72 - 209 9e-15 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 72 - 234 5.9e-12 TEP-1 second beta-propeller
WD40_Gbeta PF25391 73 - 212 1e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 94 - 202 1.2e-08 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 95 - 233 7.4e-10 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 96 - 217 1.4e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 96 - 293 2.8e-30 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 151 - 297 2.7e-15 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 183 - 298 5.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 189 - 303 3.4e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 196 - 311 3.9e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 204 - 317 8.5e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 225 - 297 2e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 235 - 298 3.3e-08 WDR5 beta-propeller domain
WD40 PF00400 258 - 292 1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 863
AciI CCGC 2 cut(s) 918, 987
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 2 cut(s) 383, 919
AcsI RAATTY 2 cut(s) 412, 634
AcuI CTGAAG 2 cut(s) 585, 668
AcvI CACGTG 1 cut(s) 925
AdeI CACNNNGTG 1 cut(s) 811
AfiI CCNNNNNNNGG 2 cut(s) 701, 901
AflII CTTAAG 1 cut(s) 466
AgeI ACCGGT 1 cut(s) 485
AgsI TTSAA 5 cut(s) 158, 284, 379, 426, 677
AhlI ACTAGT 1 cut(s) 557
AjnI CCWGG 2 cut(s) 418, 823
AluBI AGCT 7 cut(s) 8, 221, 471, 654, 931, 959, 995
AluI AGCT 7 cut(s) 8, 221, 471, 654, 931, 959, 995
Alw21I GWGCWC 3 cut(s) 10, 744, 807
Alw26I GTCTC 2 cut(s) 125, 971
Alw44I GTGCAC 2 cut(s) 740, 803
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 224
AoxI GGCC 3 cut(s) 383, 797, 919
ApaLI GTGCAC 2 cut(s) 740, 803
ApeKI GCWGC 2 cut(s) 186, 212
ApoI RAATTY 2 cut(s) 412, 634
AsiGI ACCGGT 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 2 cut(s) 95, 823
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 4 cut(s) 76, 149, 320, 500
AsuII TTCGAA 1 cut(s) 107
AxyI CCTNAGG 2 cut(s) 844, 900
BaeGI GKGCMC 2 cut(s) 744, 807
BanII GRGCYC 1 cut(s) 10
BbrPI CACGTG 1 cut(s) 925
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 3 cut(s) 10, 744, 807
BbvCI CCTCAGC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 198, 199
BccI CCATC 4 cut(s) 68, 572, 596, 903
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 2 cut(s) 420, 825
BcoDI GTCTC 2 cut(s) 125, 971
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 2 cut(s) 558, 609
BfoI RGCGCY 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 466
BisI GCNGC 4 cut(s) 187, 213, 919, 987
BlsI GCNGC 4 cut(s) 188, 214, 920, 988
Bme1390I CCNGG 2 cut(s) 420, 825
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 1 cut(s) 693
BmrFI CCNGG 2 cut(s) 420, 825
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 1 cut(s) 692
BmuI ACTGGG 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 861
BpmI CTGGAG 3 cut(s) 54, 741, 783
Bpu10I CCTNAGC 1 cut(s) 217
Bpu14I TTCGAA 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 925
BsaBI GATNNNNATC 2 cut(s) 108, 378
BsaJI CCNNGG 2 cut(s) 22, 418
BsaWI WCCGGW 2 cut(s) 61, 485
Bsc4I CCNNNNNNNGG 2 cut(s) 701, 901
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 6 cut(s) 139, 146, 346, 551, 766, 800
Bse21I CCTNAGG 2 cut(s) 844, 900
Bse3DI GCAATG 2 cut(s) 676, 714
Bse8I GATNNNNATC 2 cut(s) 108, 378
BseBI CCWGG 2 cut(s) 420, 825
BseDI CCNNGG 2 cut(s) 22, 418
BseGI GGATG 2 cut(s) 583, 895
BseJI GATNNNNATC 2 cut(s) 108, 378
BseLI CCNNNNNNNGG 2 cut(s) 701, 901
BseMI GCAATG 2 cut(s) 676, 714
BseMII CTCAG 5 cut(s) 231, 309, 858, 864, 891
BseNI ACTGG 6 cut(s) 139, 146, 346, 551, 766, 800
BseRI GAGGAG 3 cut(s) 738, 917, 956
BseSI GKGCMC 2 cut(s) 744, 807
BseXI GCAGC 2 cut(s) 198, 199
BshFI GGCC 3 cut(s) 385, 799, 921
BshTI ACCGGT 1 cut(s) 485
BsiHKAI GWGCWC 3 cut(s) 10, 744, 807
BsiSI CCGG 2 cut(s) 62, 486
BslFI GGGAC 5 cut(s) 21, 163, 518, 532, 638
BslI CCNNNNNNNGG 2 cut(s) 701, 901
BsmAI GTCTC 2 cut(s) 125, 971
BsmFI GGGAC 5 cut(s) 21, 163, 518, 532, 638
BsmI GAATGC 2 cut(s) 28, 256
BsnI GGCC 3 cut(s) 385, 799, 921
Bsp119I TTCGAA 1 cut(s) 107
Bsp1286I GDGCHC 3 cut(s) 10, 744, 807
Bsp143I GATC 1 cut(s) 605
BspACI CCGC 2 cut(s) 918, 987
BspANI GGCC 3 cut(s) 385, 799, 921
BspCNI CTCAG 5 cut(s) 230, 310, 857, 863, 892
BspLI GGNNCC 1 cut(s) 693
BspPI GGATC 1 cut(s) 613
BspQI GCTCTTC 1 cut(s) 644
BspT104I TTCGAA 1 cut(s) 107
BspTI CTTAAG 1 cut(s) 466
BsrDI GCAATG 2 cut(s) 676, 714
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 6 cut(s) 139, 146, 346, 551, 766, 800
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 22, 418
BssMI GATC 1 cut(s) 605
Bst2UI CCWGG 2 cut(s) 420, 825
Bst4CI ACNGT 4 cut(s) 332, 436, 704, 865
Bst6I CTCTTC 2 cut(s) 644, 762
BstAFI CTTAAG 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 925
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 6 cut(s) 217, 318, 844, 850, 900, 914
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 2 cut(s) 583, 895
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 823
BstKTI GATC 1 cut(s) 608
BstMAI GTCTC 2 cut(s) 125, 971
BstMBI GATC 1 cut(s) 605
BstMWI GCNNNNNNNGC 4 cut(s) 209, 218, 915, 992
BstNI CCWGG 2 cut(s) 420, 825
BstNSI RCATGY 2 cut(s) 309, 664
BstSCI CCNGG 2 cut(s) 418, 823
BstSLI GKGCMC 2 cut(s) 744, 807
BstV1I GCAGC 2 cut(s) 198, 199
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 1 cut(s) 605
BstYI RGATCY 1 cut(s) 605
Bsu36I CCTNAGG 2 cut(s) 844, 900
BsuRI GGCC 3 cut(s) 385, 799, 921
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 2 cut(s) 583, 895
BtsI GCAGTG 2 cut(s) 234, 805
BtsIMutI CAGTG 5 cut(s) 234, 339, 805, 807, 982
Cac8I GCNNGC 1 cut(s) 97
CaiI CAGNNNCTG 1 cut(s) 224
CfoI GCGC 2 cut(s) 95, 823
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 797
CseI GACGC 2 cut(s) 398, 635
CspAI ACCGGT 1 cut(s) 485
CviAII CATG 2 cut(s) 306, 661
DdeI CTNAG 6 cut(s) 217, 318, 844, 850, 900, 914
DpnI GATC 1 cut(s) 607
DpnII GATC 1 cut(s) 605
DraIII CACNNNGTG 1 cut(s) 811
DrdI GACNNNNNNGTC 1 cut(s) 863
DseDI GACNNNNNNGTC 1 cut(s) 863
EaeI YGGCCR 2 cut(s) 383, 919
Eam1104I CTCTTC 2 cut(s) 644, 762
EarI CTCTTC 2 cut(s) 644, 762
Ecl136II GAGCTC 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 10
Eco53kI GAGCTC 1 cut(s) 8
Eco57I CTGAAG 2 cut(s) 585, 668
Eco72I CACGTG 1 cut(s) 925
Eco81I CCTNAGG 2 cut(s) 844, 900
EcoICRI GAGCTC 1 cut(s) 8
EcoRI GAATTC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 418, 823
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 1 cut(s) 10
FaeI CATG 2 cut(s) 309, 664
FaqI GGGAC 5 cut(s) 21, 163, 518, 532, 638
FatI CATG 2 cut(s) 305, 660
Fnu4HI GCNGC 4 cut(s) 187, 213, 919, 987
FokI GGATG 2 cut(s) 590, 882
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 4 cut(s) 187, 213, 919, 987
FspBI CTAG 2 cut(s) 558, 609
GlaI GCGC 2 cut(s) 94, 822
GluI GCNGC 4 cut(s) 187, 213, 919, 987
GsuI CTGGAG 3 cut(s) 54, 741, 783
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 385, 799, 921
HapII CCGG 2 cut(s) 62, 486
HgaI GACGC 2 cut(s) 398, 635
HhaI GCGC 2 cut(s) 95, 823
Hin1II CATG 2 cut(s) 309, 664
Hin6I GCGC 2 cut(s) 93, 821
HinP1I GCGC 2 cut(s) 93, 821
HindIII AAGCTT 1 cut(s) 929
HinfI GANTC 6 cut(s) 67, 104, 109, 379, 677, 833
HpaII CCGG 2 cut(s) 62, 486
HphI GGTGA 4 cut(s) 76, 149, 320, 500
Hpy166II GTNNAC 4 cut(s) 335, 742, 805, 892
Hpy188I TCNGA 5 cut(s) 114, 319, 366, 853, 869
Hpy188III TCNNGA 5 cut(s) 71, 409, 575, 757, 899
Hpy8I GTNNAC 4 cut(s) 335, 742, 805, 892
Hpy99I CGWCG 2 cut(s) 44, 47
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 4 cut(s) 332, 436, 704, 865
HpyCH4IV ACGT 1 cut(s) 924
HpyCH4V TGCA 8 cut(s) 186, 254, 274, 305, 477, 669, 742, 805
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 218, 915, 992
HpyF3I CTNAG 6 cut(s) 217, 318, 844, 850, 900, 914
HpySE526I ACGT 1 cut(s) 924
Hsp92II CATG 2 cut(s) 309, 664
HspAI GCGC 2 cut(s) 93, 821
Kzo9I GATC 1 cut(s) 605
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 3 cut(s) 5, 13, 170
Lsp1109I GCAGC 2 cut(s) 198, 199
LweI GCATC 1 cut(s) 692
MaeI CTAG 2 cut(s) 558, 609
MaeII ACGT 1 cut(s) 924
MaeIII GTNAC 7 cut(s) 64, 137, 488, 515, 553, 704, 779
MalI GATC 1 cut(s) 607
MboI GATC 1 cut(s) 605
MboII GAAGA 4 cut(s) 661, 740, 779, 866
MflI RGATCY 1 cut(s) 605
MhlI GDGCHC 3 cut(s) 10, 744, 807
MluCI AATT 4 cut(s) 269, 412, 426, 634
MlyI GAGTC 2 cut(s) 61, 842
MmeI TCCRAC 1 cut(s) 669
Mph1103I ATGCAT 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 2 cut(s) 467, 615
MspCI CTTAAG 1 cut(s) 466
MspI CCGG 2 cut(s) 62, 486
MspR9I CCNGG 2 cut(s) 420, 825
Mva1269I GAATGC 2 cut(s) 28, 256
MvaI CCWGG 2 cut(s) 420, 825
MwoI GCNNNNNNNGC 4 cut(s) 209, 218, 915, 992
NdeII GATC 1 cut(s) 605
NlaIII CATG 2 cut(s) 309, 664
NlaIV GGNNCC 1 cut(s) 693
NmuCI GTSAC 4 cut(s) 64, 137, 488, 515
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 2 cut(s) 309, 664
NspV TTCGAA 1 cut(s) 107
PciSI GCTCTTC 1 cut(s) 644
PcsI WCGNNNNNNNCGW 1 cut(s) 692
PctI GAATGC 2 cut(s) 28, 256
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 104, 109, 379, 677
PinAI ACCGGT 1 cut(s) 485
PkrI GCNGC 4 cut(s) 188, 214, 920, 988
PleI GAGTC 2 cut(s) 61, 841
PmaCI CACGTG 1 cut(s) 925
PmlI CACGTG 1 cut(s) 925
PpsI GAGTC 2 cut(s) 61, 841
Ppu21I YACGTR 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 418, 823
PspCI CACGTG 1 cut(s) 925
PspGI CCWGG 2 cut(s) 418, 823
PspN4I GGNNCC 1 cut(s) 693
PspPI GGNCC 1 cut(s) 797
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 605
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 2 cut(s) 467, 615
SatI GCNGC 4 cut(s) 187, 213, 919, 987
Sau3AI GATC 1 cut(s) 605
Sau96I GGNCC 1 cut(s) 797
SchI GAGTC 2 cut(s) 61, 842
ScrFI CCNGG 2 cut(s) 420, 825
SduI GDGCHC 3 cut(s) 10, 744, 807
SfaNI GCATC 1 cut(s) 692
SfuI TTCGAA 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
SpeI ACTAGT 1 cut(s) 557
Sse9I AATT 4 cut(s) 269, 412, 426, 634
SsiI CCGC 2 cut(s) 918, 987
SspMI CTAG 2 cut(s) 558, 609
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 418, 823
TaaI ACNGT 4 cut(s) 332, 436, 704, 865
TaiI ACGT 1 cut(s) 927
TaqI TCGA 4 cut(s) 45, 107, 372, 438
TasI AATT 4 cut(s) 269, 412, 426, 634
TauI GCSGC 2 cut(s) 921, 989
TfiI GAWTC 4 cut(s) 104, 109, 379, 677
Tru1I TTAA 2 cut(s) 467, 615
Tru9I TTAA 2 cut(s) 467, 615
TscAI CASTG 5 cut(s) 241, 346, 807, 812, 982
TseFI GTSAC 4 cut(s) 64, 137, 488, 515
TseI GCWGC 2 cut(s) 186, 212
Tsp45I GTSAC 4 cut(s) 64, 137, 488, 515
TspDTI ATGAA 4 cut(s) 282, 437, 518, 703
TspGWI ACGGA 2 cut(s) 873, 959
TspRI CASTG 5 cut(s) 241, 346, 807, 812, 982
Vha464I CTTAAG 1 cut(s) 466
VneI GTGCAC 2 cut(s) 740, 803
XapI RAATTY 2 cut(s) 412, 634
XceI RCATGY 2 cut(s) 309, 664
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 2 cut(s) 558, 609
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.