MD15G1178400.v1.1

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009140
Physical Location & Seq
Forward (+)
14051940 .. 14055805
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1178400.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGATAATAAGAAGAAGGTGGCGGTTCCGCTGGTTTGCCACGGCCATTCGCGTCCGGTGGTGGATCTGTTTTACAGTCCGGTTACTCCTGATGGGTTCTTCCTCATCAGTGCCAGTAAGGATTCGAGTCCAATGCTGAGAAACGGAGAGACTGGAGATTGGATTGGAACATTTGAAGGGCACAAAGGTGCAGTGTGGAGTTGCTGCTTGGATACTAATGCCCTACGTGCTGCAACTGCTTCTGCAGATTTCTCTGCGAAAGTTTGGGATGCATTATCAGGAGATGTGCTACACTCATTTGAACACAAGCATATTGTTCGAGCTTGTGCCTTTTCAGAGGATACACACCTTTTGCTAACCGGGGGAGTAGAGAAAGTTATCCGTATATATGATTTGAATCGTCCGGATGCCCCTCCAAGAGAAGTGGATAAATCCCCAGGTTCAGTCAGAACTGCTGCGTGGCTCCACAGTGACCAGACCATACTAAGTTCTTGTACTGATATGGGAGGTGTCAGATTATGGGATGTAAGAACTGGTAAAATAGTCCAAACACTGGAGACCAAATCATCCGTGACCAGTTCTGAAGTTAGTCAAGATGGTCGTTATATTACTACTGCTGATGGGTCGACTGTGAAGTTTTGGGATGCCAACTACTTTGGGTTGGTGAAGAGCTACAATATGCCGTGCACAGTAGAATCAGCTTCACTGGAACCAAAGTTTGGGAACAAGTTCATTGCTGGGGGAGAAGATATGTGGGTTCATGTGTTTGATTTCCATACCGGTGAAGAGATTGCATGCAACAAGGGTCACCACGGTCCTGTTCACTGTCTGCGTTTCTCGCCCGGAGGGGAGTCATATGCCTCGGGATCTGAAGATGGAACCATAAGAATATGGCAGACCCCTTTGACTCCAGAAGAAACCGAAGCACTGGTAGCAAATGGGCCAGTTGGAAACGTGGAGGTACCCGCAGAAGAGGTTTCTCGCAAGATTGAGGGCTTCCACATCACGGACGAGGGGAAAGCGAAAGAGAAGGAAGAGGCGGCAGTGAATGAGTAA

Protein Analysis

352

Amino Acids

38.24

Weight (kDa)

5.32

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 10 - 298 2e-45 EIF3I
Beta-prop_WDR3_2nd PF25172 13 - 131 3.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 13 - 133 2.2e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 14 - 227 4.3e-28 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 30 - 114 5.1e-07 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 31 - 216 2.5e-13 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 34 - 227 2.9e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 35 - 133 2e-06 G protein beta WD-40 repeat protein
WD40 PF00400 55 - 90 5.6e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 79 - 216 1.8e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 99 - 209 6.1e-09 WDR55
Beta-prop_TEP1_2nd PF25047 101 - 221 9.7e-12 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 102 - 239 2.6e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 102 - 300 1.1e-32 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 106 - 218 9.6e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 147 - 215 3.9e-06 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 158 - 298 2.4e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 191 - 300 1.1e-08 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 191 - 300 2.7e-08 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 202 - 301 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 231 - 302 3.7e-10 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 237 - 299 3e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 241 - 300 5.6e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 244 - 300 9e-10 WDR5 beta-propeller domain
WD40 PF00400 264 - 298 6.3e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 961
AccB7I CCANNNNNTGG 2 cut(s) 553, 719
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 52
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 4 cut(s) 23, 29, 966, 1040
AclWI GGATC 2 cut(s) 72, 874
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 603, 891
AfaI GTAC 2 cut(s) 496, 963
AfiI CCNNNNNNNGG 3 cut(s) 553, 719, 1006
AgeI ACCGGT 1 cut(s) 779
AgsI TTSAA 3 cut(s) 176, 302, 397
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 702, 734
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 3 cut(s) 323, 672, 701
AluI AGCT 3 cut(s) 323, 672, 701
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 2 cut(s) 143, 551
Alw44I GTGCAC 1 cut(s) 685
AlwI GGATC 2 cut(s) 72, 874
Ama87I CYCGRG 1 cut(s) 862
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 2 cut(s) 43, 941
ApaLI GTGCAC 1 cut(s) 685
ApeKI GCWGC 3 cut(s) 204, 230, 455
AsiGI ACCGGT 1 cut(s) 779
Asp700I GAANNNNTTC 1 cut(s) 728
Asp718I GGTACC 1 cut(s) 961
AspS9I GGNCC 2 cut(s) 815, 941
AsuC2I CCSGG 2 cut(s) 361, 843
AsuHPI GGTGA 3 cut(s) 676, 794, 800
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 1 cut(s) 815
BaeGI GKGCMC 2 cut(s) 183, 689
BanI GGYRCC 1 cut(s) 961
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 3 cut(s) 191, 217, 442
BccI CCATC 4 cut(s) 86, 590, 614, 869
BceAI ACGGC 2 cut(s) 58, 667
BcgI CGANNNNNNTGC 4 cut(s) 115, 149, 299, 333
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 2 cut(s) 205, 334
BcnI CCSGG 2 cut(s) 361, 843
BcoDI GTCTC 2 cut(s) 143, 551
BfmI CTRYAG 1 cut(s) 243
BfuI GTATCC 2 cut(s) 205, 334
BisI GCNGC 4 cut(s) 205, 231, 456, 1041
BlsI GCNGC 4 cut(s) 206, 232, 457, 1042
Bme1390I CCNGG 3 cut(s) 361, 438, 843
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 815, 941
BmiI GGNNCC 5 cut(s) 27, 464, 711, 880, 963
BmrFI CCNGG 3 cut(s) 361, 438, 843
BmsI GCATC 3 cut(s) 259, 397, 634
BpmI CTGGAG 3 cut(s) 174, 575, 894
BpuMI CCSGG 2 cut(s) 361, 843
BsaAI YACGTR 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BsaWI WCCGGW 4 cut(s) 55, 79, 403, 779
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 719, 1006
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 8 cut(s) 114, 157, 538, 558, 576, 711, 933, 944
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BseGI GGATG 5 cut(s) 274, 412, 529, 566, 649
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 553, 719, 1006
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 8 cut(s) 114, 157, 538, 558, 576, 711, 933, 944
BseSI GKGCMC 2 cut(s) 183, 689
BseXI GCAGC 3 cut(s) 191, 217, 442
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 45, 943
BshNI GGYRCC 1 cut(s) 961
BshTI ACCGGT 1 cut(s) 779
BsiHKAI GWGCWC 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 6 cut(s) 56, 80, 360, 404, 780, 843
BslI CCNNNNNNNGG 3 cut(s) 553, 719, 1006
BsmAI GTCTC 2 cut(s) 143, 551
BsnI GGCC 2 cut(s) 45, 943
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 2 cut(s) 183, 689
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 2 cut(s) 64, 866
BspACI CCGC 4 cut(s) 23, 29, 966, 1040
BspANI GGCC 2 cut(s) 45, 943
BspCNI CTCAG 1 cut(s) 129
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 5 cut(s) 27, 464, 711, 880, 963
BspMAI CTGCAG 1 cut(s) 247
BspPI GGATC 2 cut(s) 72, 874
BspQI GCTCTTC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 961
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 1 cut(s) 779
BsrI ACTGG 8 cut(s) 114, 157, 538, 558, 576, 711, 933, 944
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 5 cut(s) 40, 360, 436, 811, 861
BssMI GATC 2 cut(s) 64, 866
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 6 cut(s) 77, 470, 631, 691, 815, 827
Bst6I CTCTTC 4 cut(s) 662, 780, 966, 1029
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 796
BstDEI CTNAG 2 cut(s) 137, 485
BstDSI CCRYGG 2 cut(s) 40, 811
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 5 cut(s) 274, 412, 529, 566, 649
BstFNI CGCG 1 cut(s) 52
BstKTI GATC 2 cut(s) 67, 869
BstMAI GTCTC 2 cut(s) 143, 551
BstMBI GATC 2 cut(s) 64, 866
BstMWI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 932
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 798
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 3 cut(s) 359, 436, 841
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 2 cut(s) 183, 689
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 191, 217, 442
BstX2I RGATCY 2 cut(s) 64, 866
BstYI RGATCY 2 cut(s) 64, 866
BsuI GTATCC 2 cut(s) 205, 334
BsuRI GGCC 2 cut(s) 45, 943
BtgI CCRYGG 2 cut(s) 40, 811
BtsCI GGATG 5 cut(s) 274, 412, 529, 566, 649
BtsI GCAGTG 2 cut(s) 198, 1050
BtsIMutI CAGTG 8 cut(s) 115, 198, 475, 551, 704, 823, 926, 1050
Cac8I GCNNGC 1 cut(s) 796
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 815, 941
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 495, 962
CspAI ACCGGT 1 cut(s) 779
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 2 cut(s) 761, 795
CviJI RGCY 7 cut(s) 45, 323, 463, 672, 701, 943, 996
CviKI_1 RGCY 7 cut(s) 45, 323, 463, 672, 701, 943, 996
CviQI GTAC 2 cut(s) 495, 962
DdeI CTNAG 2 cut(s) 137, 485
DpnI GATC 2 cut(s) 66, 868
DpnII GATC 2 cut(s) 64, 866
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 4 cut(s) 662, 780, 966, 1029
EarI CTCTTC 4 cut(s) 662, 780, 966, 1029
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 815
Eco57I CTGAAG 2 cut(s) 603, 891
Eco88I CYCGRG 1 cut(s) 862
Eco91I GGTNACC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 274
FaeI CATG 2 cut(s) 764, 798
FatI CATG 2 cut(s) 760, 794
FauI CCCGC 1 cut(s) 973
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 4 cut(s) 205, 231, 456, 1041
FokI GGATG 5 cut(s) 281, 419, 536, 553, 656
Fsp4HI GCNGC 4 cut(s) 205, 231, 456, 1041
GluI GCNGC 4 cut(s) 205, 231, 456, 1041
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 3 cut(s) 174, 575, 894
HaeIII GGCC 2 cut(s) 45, 943
HapII CCGG 6 cut(s) 56, 80, 360, 404, 780, 843
HgaI GACGC 1 cut(s) 41
Hin1II CATG 2 cut(s) 764, 798
HincII GTYRAC 1 cut(s) 627
HindII GTYRAC 1 cut(s) 627
HinfI GANTC 6 cut(s) 122, 127, 397, 695, 851, 907
HpaII CCGG 6 cut(s) 56, 80, 360, 404, 780, 843
HphI GGTGA 3 cut(s) 676, 794, 800
Hpy166II GTNNAC 3 cut(s) 627, 687, 823
Hpy188I TCNGA 5 cut(s) 337, 449, 515, 583, 871
Hpy188III TCNNGA 6 cut(s) 89, 279, 404, 593, 864, 911
Hpy8I GTNNAC 3 cut(s) 627, 687, 823
HpyAV CCTTC 3 cut(s) 10, 170, 1024
HpyCH4III ACNGT 6 cut(s) 77, 470, 631, 691, 815, 827
HpyCH4IV ACGT 2 cut(s) 226, 954
HpyCH4V TGCA 7 cut(s) 191, 233, 245, 272, 687, 794, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 932
HpyF3I CTNAG 2 cut(s) 137, 485
HpySE526I ACGT 2 cut(s) 226, 954
Hsp92II CATG 2 cut(s) 764, 798
Kpn2I TCCGGA 1 cut(s) 403
KpnI GGTACC 1 cut(s) 965
Kzo9I GATC 2 cut(s) 64, 866
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 3 cut(s) 191, 217, 442
LweI GCATC 3 cut(s) 259, 397, 634
MaeII ACGT 2 cut(s) 226, 954
MaeIII GTNAC 4 cut(s) 82, 470, 571, 806
MalI GATC 2 cut(s) 66, 868
MboI GATC 2 cut(s) 64, 866
MboII GAAGA 9 cut(s) 25, 91, 679, 758, 797, 884, 926, 983, 1046
MflI RGATCY 2 cut(s) 64, 866
MhlI GDGCHC 2 cut(s) 183, 689
MlyI GAGTC 3 cut(s) 136, 860, 901
MmeI TCCRAC 1 cut(s) 928
Mph1103I ATGCAT 1 cut(s) 274
MroI TCCGGA 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 728
MslI CAYNNNNRTG 2 cut(s) 186, 780
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 6 cut(s) 56, 80, 360, 404, 780, 843
MspR9I CCNGG 3 cut(s) 361, 438, 843
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 4 cut(s) 227, 236, 838, 932
NciI CCSGG 2 cut(s) 361, 843
NdeI CATATG 1 cut(s) 856
NdeII GATC 2 cut(s) 64, 866
NlaIII CATG 2 cut(s) 764, 798
NlaIV GGNNCC 5 cut(s) 27, 464, 711, 880, 963
NmuCI GTSAC 3 cut(s) 470, 571, 806
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 798
OliI CACNNNNGTG 1 cut(s) 186
PaeI GCATGC 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 3 cut(s) 122, 397, 695
PflMI CCANNNNNTGG 2 cut(s) 553, 719
PinAI ACCGGT 1 cut(s) 779
PkrI GCNGC 4 cut(s) 206, 232, 457, 1042
PleI GAGTC 3 cut(s) 135, 859, 901
PpsI GAGTC 3 cut(s) 135, 859, 901
Ppu21I YACGTR 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 5 cut(s) 27, 464, 711, 880, 963
PspPI GGNCC 2 cut(s) 815, 941
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 2 cut(s) 64, 866
RsaI GTAC 2 cut(s) 496, 963
RsaNI GTAC 2 cut(s) 495, 962
RseI CAYNNNNRTG 2 cut(s) 186, 780
SalI GTCGAC 1 cut(s) 625
SapI GCTCTTC 1 cut(s) 662
SatI GCNGC 4 cut(s) 205, 231, 456, 1041
Sau3AI GATC 2 cut(s) 64, 866
Sau96I GGNCC 2 cut(s) 815, 941
SchI GAGTC 3 cut(s) 136, 860, 901
ScrFI CCNGG 3 cut(s) 361, 438, 843
SduI GDGCHC 2 cut(s) 183, 689
SfaNI GCATC 3 cut(s) 259, 397, 634
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 186, 780
SphI GCATGC 1 cut(s) 798
SsiI CCGC 4 cut(s) 23, 29, 966, 1040
StyD4I CCNGG 3 cut(s) 359, 436, 841
TaaI ACNGT 6 cut(s) 77, 470, 631, 691, 815, 827
TaiI ACGT 2 cut(s) 229, 957
TaqI TCGA 3 cut(s) 125, 319, 626
TatI WGTACW 1 cut(s) 494
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 3 cut(s) 122, 397, 695
TscAI CASTG 8 cut(s) 115, 198, 475, 558, 711, 830, 933, 1050
TseFI GTSAC 3 cut(s) 470, 571, 806
TseI GCWGC 3 cut(s) 204, 230, 455
Tsp45I GTSAC 3 cut(s) 470, 571, 806
TspDTI ATGAA 2 cut(s) 721, 749
TspGWI ACGGA 4 cut(s) 159, 371, 559, 1022
TspRI CASTG 8 cut(s) 115, 198, 475, 558, 711, 830, 933, 1050
Van91I CCANNNNNTGG 2 cut(s) 553, 719
VneI GTGCAC 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 815
XceI RCATGY 1 cut(s) 798
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.