MD02G1038200.v1.1

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009140
Physical Location & Seq
Forward (+)
2905613 .. 2908428
2816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1038200.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGATAATAAGAAGAAGGTGGCGGTTCCGTTGGTCTGCCACGGCCATTCGCGTCCGGTGGTGGATCTGTTTTACAGTCCGGTTACTCCTGATGGGTTCTTTCTCATCAGTGCCAGTAAGGATTCGAGTCCTATGCTGAGAAATGGAGAGACTGGAGATTGGGTTGGAACATTTGAAGGGCATAAAGGCGCAGTGTGGAGTTGCTGCTTGGATACTAACGCCCTACGTGCTGCAACTGCTTCCGCAGATTTCTCTGCGAAAGTTTGGGATGCATTAACAGGACATGTTCTACACTCATTTGAACACAAGCACATTGTTCGAGCCTGTGCTTTCGCAGAGGATACACACCTTTTGCTTACTGGAGGAGTAGAGAAAGTTATTCGTATATATGATTTGAATCGTCCAGATGCCCCTCCAAGAGAAGTGGATAAATCCCCTGGTTCAGTCAGAACTGCTGCATGGCTCCACAGTGACCAGACCATACTAAGTTCTTGTACTGATATGGGTGGTGTCAGATTATGGGATGTAAGAACCGGTAAAATCGTCCAAACTCTTGAGACCAAATCATCTGTGACCAGTTCTGAAGTGAGTCAAGATGGTCGTTATATCACTACTGCTGATGGGTCTACTGTGAAGTTTTGGGATGCAAATTAG

Protein Analysis

218

Amino Acids

23.66

Weight (kDa)

6.29

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 3 - 104 5.2e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 10 - 206 6.3e-29 EIF3I
Beta-prop_WDR3_2nd PF25172 13 - 131 1.5e-07 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 14 - 216 5e-27 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 14 - 132 6.8e-14 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 30 - 134 3.8e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 34 - 215 2.4e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 35 - 132 5.9e-07 G protein beta WD-40 repeat protein
WD40 PF00400 55 - 90 2.8e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 65 - 216 3.5e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 79 - 216 6.6e-09 G protein beta WD-40 repeat protein
WDR55 PF24796 98 - 209 6.8e-09 WDR55
Beta-prop_TEP1_2nd PF25047 100 - 216 5.1e-11 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 102 - 209 4.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 102 - 215 2.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 102 - 216 4.3e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 106 - 215 3.1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 147 - 215 1.7e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 52
AciI CCGC 2 cut(s) 23, 243
AclWI GGATC 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 1 cut(s) 496
AflIII ACRYGT 1 cut(s) 283
AgeI ACCGGT 1 cut(s) 533
AgsI TTSAA 3 cut(s) 176, 302, 397
AjnI CCWGG 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 143, 551
AlwI GGATC 1 cut(s) 72
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 3 cut(s) 204, 230, 455
AsiGI ACCGGT 1 cut(s) 533
AspLEI GCGC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 191, 217, 442
BccI CCATC 3 cut(s) 86, 590, 614
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 4 cut(s) 115, 149, 299, 333
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 2 cut(s) 205, 334
BcoDI GTCTC 2 cut(s) 143, 551
BfuI GTATCC 2 cut(s) 205, 334
BisI GCNGC 3 cut(s) 205, 231, 456
BlsI GCNGC 3 cut(s) 206, 232, 457
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 27, 464
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 3 cut(s) 259, 397, 634
BpmI CTGGAG 2 cut(s) 174, 381
BpuEI CTTGAG 1 cut(s) 575
BsaAI YACGTR 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 40, 436
BsaWI WCCGGW 3 cut(s) 55, 79, 533
BsaXI ACNNNNNCTCC 2 cut(s) 147, 177
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 4 cut(s) 114, 157, 364, 576
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 40, 436
BseGI GGATG 3 cut(s) 274, 529, 649
BseJI GATNNNNATC 1 cut(s) 396
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 4 cut(s) 114, 157, 364, 576
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 191, 217, 442
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 1 cut(s) 45
BshTI ACCGGT 1 cut(s) 533
BsiSI CCGG 3 cut(s) 56, 80, 534
BsmAI GTCTC 2 cut(s) 143, 551
BsnI GGCC 1 cut(s) 45
Bso31I GGTCTC 1 cut(s) 551
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 2 cut(s) 23, 243
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 27, 464
BspPI GGATC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 551
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 4 cut(s) 114, 157, 364, 576
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 2 cut(s) 40, 436
BssMI GATC 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 3 cut(s) 77, 470, 631
BstBAI YACGTR 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 137, 485
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 274, 529, 649
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 2 cut(s) 143, 551
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 227, 236
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 436
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 3 cut(s) 191, 217, 442
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
BsuI GTATCC 2 cut(s) 205, 334
BsuRI GGCC 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 274, 529, 649
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 3 cut(s) 115, 198, 475
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 533
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 495
CspAI ACCGGT 1 cut(s) 533
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 2 cut(s) 284, 459
CviJI RGCY 3 cut(s) 45, 323, 463
CviKI_1 RGCY 3 cut(s) 45, 323, 463
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 2 cut(s) 137, 485
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 551
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 274
FaeI CATG 2 cut(s) 287, 462
FatI CATG 2 cut(s) 283, 458
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 3 cut(s) 205, 231, 456
FokI GGATG 2 cut(s) 281, 536
Fsp4HI GCNGC 3 cut(s) 205, 231, 456
GlaI GCGC 1 cut(s) 190
GluI GCNGC 3 cut(s) 205, 231, 456
GsuI CTGGAG 2 cut(s) 174, 381
HaeIII GGCC 1 cut(s) 45
HapII CCGG 3 cut(s) 56, 80, 534
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 287, 462
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HinfI GANTC 4 cut(s) 122, 127, 397, 589
HpaII CCGG 3 cut(s) 56, 80, 534
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 3 cut(s) 449, 515, 583
Hpy188III TCNNGA 4 cut(s) 89, 404, 554, 593
Hpy8I GTNNAC 1 cut(s) 627
HpyAV CCTTC 2 cut(s) 10, 170
HpyCH4III ACNGT 3 cut(s) 77, 470, 631
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 4 cut(s) 233, 272, 458, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 236
HpyF3I CTNAG 2 cut(s) 137, 485
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 2 cut(s) 287, 462
HspAI GCGC 1 cut(s) 189
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 3 cut(s) 191, 217, 442
LweI GCATC 3 cut(s) 259, 397, 634
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 3 cut(s) 82, 470, 571
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 1 cut(s) 25
MflI RGATCY 1 cut(s) 64
MluCI AATT 1 cut(s) 649
MlyI GAGTC 2 cut(s) 136, 598
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 3 cut(s) 331, 356, 423
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 1 cut(s) 275
MspI CCGG 3 cut(s) 56, 80, 534
MspR9I CCNGG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 2 cut(s) 227, 236
NdeII GATC 1 cut(s) 64
NlaIII CATG 2 cut(s) 287, 462
NlaIV GGNNCC 2 cut(s) 27, 464
NmuCI GTSAC 2 cut(s) 470, 571
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 287
PciI ACATGT 1 cut(s) 283
PfeI GAWTC 2 cut(s) 122, 397
PinAI ACCGGT 1 cut(s) 533
PkrI GCNGC 3 cut(s) 206, 232, 457
PleI GAGTC 2 cut(s) 135, 597
PpsI GAGTC 2 cut(s) 135, 597
Ppu21I YACGTR 1 cut(s) 227
PscI ACATGT 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 27, 464
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 3 cut(s) 205, 231, 456
Sau3AI GATC 1 cut(s) 64
SchI GAGTC 2 cut(s) 136, 598
ScrFI CCNGG 1 cut(s) 438
SetI ASST 3 cut(s) 21, 229, 351
SfaNI GCATC 3 cut(s) 259, 397, 634
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 1 cut(s) 649
SsiI CCGC 2 cut(s) 23, 243
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 3 cut(s) 77, 470, 631
TaiI ACGT 1 cut(s) 229
TaqI TCGA 2 cut(s) 125, 319
TasI AATT 1 cut(s) 649
TatI WGTACW 1 cut(s) 494
TfiI GAWTC 2 cut(s) 122, 397
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 3 cut(s) 115, 198, 475
TseFI GTSAC 2 cut(s) 470, 571
TseI GCWGC 3 cut(s) 204, 230, 455
Tsp45I GTSAC 2 cut(s) 470, 571
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 3 cut(s) 115, 198, 475
XceI RCATGY 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 626
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.