AT1G52730

Contains the following InterPro domains WD40 repeat 2 (InterPro IPR019782), WD40 repeat-like-containing domain (InterPro IPR011046), WD40 repeat, conserved site (InterPro IPR019775), WD40-repeat-containing domain (InterPro IPR017986), WD40 YVTN repeat-like-containing domain (InterPro IPR015943), WD40 repeat (InterPro IPR001680), WD40 repeat, subgroup (InterPro IPR019781)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009140
Physical Location & Seq
Forward (+)
19642452 .. 19645211
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G52730.3

Sequence Viewer

Length: 1032 bp
ATGGAGAAGAAGAAGGTGACGATTCCACAAGTGTGTCATGGCCATTCAAGACCTGTTGTTGATTTGTTCTACAGTCCAATTACTCCTGACGGTTTCTTCCTCATCAGCGCTAGCAAAGATTCGCATCCAATGTTGAGAAATGGGGAAACTGGAGACTGGATTGGTACATTTGAAGGGCATAAAGGTGCAGTTTGGAGTTCTTGTCTGGATAACAATGCATTACGTGCTGCATCTGCTTCTGCCGATTTTTCAGCCAAACTTTGGGATGCGTTAACAGGGGATGTGCTGCATTCTTTTGAGCACAAGCATATTGTCCGAGCATGCGCCTTCTCAGAGGATACAAAATCGTTGCTCACAGGAGGGTTTGAGAAAATTCTTCGTGTTTTTGACATGAATCGGTTGGATGCACCTCCTACAGAAGTTGATAAATCTCCTGGTTCTATCAGAACCCTCACATGGCTTCACAGTGATCAAACAATATTAAGTTCATGTACTGATATTGGCGGTGTAAGGTTATGGGATGTGAGGAGTGGGAAAATTGTGCAAACGTTAGAAACTAAGTCTCCTGTCACCAGTGCTGAAGTGAGCCAAGATGGACGGTATATAACAACTGCTGATGGGTCAACTGTTAAATTCTGGGACGCTAACCATTTTGGACTAGTGAAGAGTTACGACATGCCCTGCAATATTGAATCTGCATCGTTGGAGCCAAAGTCTGGTGAAAAATTTGTTGCTGGTGGAGAGGATATGTGGGTCCGAGTGTTTGATTTCTACACTGGCGAAGAGATTGGATGCAACAAGGGACATCACGGTCCAGTACACTGCGTTAGGTTTACTCCCACTGGTTTATCCTATGCCTCGGGATCTGAAGACGGGACAATCAGAATCTGGCAAACAACACCAGCAAATCCCGAAGAGAACGAAACTAGCTCGAGGAGAGTGAAGCATAGCGTGGATGAAGTTTCAAAGAAGATTGAAGGCTTTCACATCAACAAAGAAGGAAAAACCGCAGAGAAACCATCTGATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
WD40

Protein Analysis

343

Amino Acids

37.75

Weight (kDa)

5.87

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 9 - 249 2.1e-33 EIF3I
Beta-prop_WDR5 PF25175 13 - 260 4.8e-30 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 13 - 131 3.4e-12 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 13 - 131 1.3e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 29 - 116 1.9e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 33 - 259 9.2e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 34 - 132 2.2e-07 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 68 - 202 1.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 77 - 239 1.3e-11 TEP-1 second beta-propeller
Beta-prop_ACSF4 PF13570 89 - 257 3.7e-06 Beta-alanine-activating enzyme, beta-propeller
WDR55 PF24796 98 - 256 2.2e-10 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 100 - 238 8.1e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 101 - 263 2.5e-21 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 101 - 253 2.1e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 146 - 214 7.2e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 810
AccB7I CCANNNNNTGG 2 cut(s) 261, 716
AciI CCGC 2 cut(s) 504, 1008
AclI AACGTT 1 cut(s) 548
AclWI GGATC 1 cut(s) 871
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 3 cut(s) 372, 632, 725
AcuI CTGAAG 2 cut(s) 600, 888
AfaI GTAC 3 cut(s) 166, 493, 819
AfeI AGCGCT 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 261, 456, 716
AgsI TTSAA 5 cut(s) 48, 173, 692, 966, 977
AhlI ACTAGT 1 cut(s) 658
AjnI CCWGG 1 cut(s) 433
AleI CACNNNNGTG 1 cut(s) 31
AluBI AGCT 1 cut(s) 930
AluI AGCT 1 cut(s) 930
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 147, 567
AlwI GGATC 1 cut(s) 871
AlwNI CAGNNNCTG 1 cut(s) 888
Ama87I CYCGRG 2 cut(s) 859, 931
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 227, 286
ApoI RAATTY 3 cut(s) 372, 632, 725
Asp700I GAANNNNTTC 1 cut(s) 981
AspLEI GCGC 2 cut(s) 110, 326
AspS9I GGNCC 2 cut(s) 754, 812
AsuHPI GGTGA 3 cut(s) 28, 562, 731
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 2 cut(s) 859, 931
AvaII GGWCC 2 cut(s) 754, 812
BalI TGGCCA 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 214, 273
BccI CCATC 3 cut(s) 587, 611, 1027
BciT130I CCWGG 1 cut(s) 435
BciVI GTATCC 1 cut(s) 331
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 2 cut(s) 147, 567
BcuI ACTAGT 1 cut(s) 658
BfaI CTAG 3 cut(s) 111, 659, 927
BfmI CTRYAG 2 cut(s) 70, 414
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 2 cut(s) 228, 287
BlsI GCNGC 2 cut(s) 229, 288
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 754, 812
BmeT110I CYCGRG 2 cut(s) 859, 931
BmgT120I GGNCC 2 cut(s) 754, 812
BmiI GGNNCC 2 cut(s) 708, 755
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 6 cut(s) 133, 239, 256, 394, 707, 782
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 123
BsaJI CCNNGG 1 cut(s) 858
BsaXI ACNNNNNCTCC 6 cut(s) 187, 217, 547, 577, 698, 728
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 456, 716
Bse1I ACTGG 6 cut(s) 154, 161, 573, 781, 815, 847
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 858
BseGI GGATG 7 cut(s) 124, 271, 286, 409, 526, 797, 961
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 261, 456, 716
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 6 cut(s) 154, 161, 573, 781, 815, 847
BseRI GAGGAG 2 cut(s) 541, 949
BseXI GCAGC 2 cut(s) 214, 273
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 2 cut(s) 859, 931
BslFI GGGAC 3 cut(s) 653, 816, 889
BslI CCNNNNNNNGG 3 cut(s) 261, 456, 716
BsmAI GTCTC 2 cut(s) 147, 567
BsmFI GGGAC 3 cut(s) 653, 816, 889
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 1 cut(s) 42
BsoBI CYCGRG 2 cut(s) 859, 931
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 2 cut(s) 469, 863
BspACI CCGC 2 cut(s) 504, 1008
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 1 cut(s) 344
BspLI GGNNCC 2 cut(s) 708, 755
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 871
BsrI ACTGG 6 cut(s) 154, 161, 573, 781, 815, 847
BssECI CCNNGG 1 cut(s) 858
BssMI GATC 2 cut(s) 469, 863
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 6 cut(s) 74, 92, 467, 600, 628, 812
Bst6I CTCTTC 3 cut(s) 659, 777, 909
BstAPI GCANNNNNTGC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 112, 322
BstDEI CTNAG 2 cut(s) 331, 558
BstF5I GGATG 7 cut(s) 124, 271, 286, 409, 526, 797, 961
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 326
BstKTI GATC 2 cut(s) 472, 866
BstMAI GTCTC 2 cut(s) 147, 567
BstMBI GATC 2 cut(s) 469, 863
BstMWI GCNNNNNNNGC 2 cut(s) 224, 233
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 2 cut(s) 324, 679
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 2 cut(s) 70, 414
BstV1I GCAGC 2 cut(s) 214, 273
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 1 cut(s) 863
BstYI RGATCY 1 cut(s) 863
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 7 cut(s) 124, 271, 286, 409, 526, 797, 961
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 5 cut(s) 472, 580, 774, 820, 840
Cac8I GCNNGC 2 cut(s) 112, 322
CaiI CAGNNNCTG 1 cut(s) 888
CfoI GCGC 2 cut(s) 110, 326
Cfr13I GGNCC 2 cut(s) 754, 812
CseI GACGC 1 cut(s) 650
Csp6I GTAC 3 cut(s) 165, 492, 818
CviAII CATG 6 cut(s) 38, 321, 391, 456, 489, 676
CviJI RGCY 7 cut(s) 42, 254, 460, 588, 709, 930, 981
CviKI_1 RGCY 7 cut(s) 42, 254, 460, 588, 709, 930, 981
CviQI GTAC 3 cut(s) 165, 492, 818
DdeI CTNAG 2 cut(s) 331, 558
DpnI GATC 2 cut(s) 471, 865
DpnII GATC 2 cut(s) 469, 863
DrdI GACNNNNNNGTC 1 cut(s) 810
DseDI GACNNNNNNGTC 1 cut(s) 810
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 3 cut(s) 659, 777, 909
EarI CTCTTC 3 cut(s) 659, 777, 909
Eco47I GGWCC 2 cut(s) 754, 812
Eco47III AGCGCT 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 600, 888
Eco88I CYCGRG 2 cut(s) 859, 931
EcoRII CCWGG 1 cut(s) 433
EcoT22I ATGCAT 1 cut(s) 220
FaeI CATG 6 cut(s) 41, 324, 394, 459, 492, 679
FaqI GGGAC 3 cut(s) 653, 816, 889
FatI CATG 6 cut(s) 37, 320, 390, 455, 488, 675
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 228, 287
FokI GGATG 7 cut(s) 111, 278, 293, 416, 533, 804, 968
Fsp4HI GCNGC 2 cut(s) 228, 287
FspBI CTAG 3 cut(s) 111, 659, 927
GlaI GCGC 2 cut(s) 109, 325
GluI GCNGC 2 cut(s) 228, 287
GsuI CTGGAG 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 42
HgaI GACGC 1 cut(s) 650
HhaI GCGC 2 cut(s) 110, 326
Hin1II CATG 6 cut(s) 41, 324, 394, 459, 492, 679
Hin6I GCGC 2 cut(s) 108, 324
HinP1I GCGC 2 cut(s) 108, 324
HincII GTYRAC 2 cut(s) 273, 624
HindII GTYRAC 2 cut(s) 273, 624
HinfI GANTC 5 cut(s) 22, 119, 394, 692, 885
HpaI GTTAAC 1 cut(s) 273
HphI GGTGA 3 cut(s) 28, 562, 731
Hpy166II GTNNAC 4 cut(s) 273, 624, 820, 834
Hpy188I TCNGA 7 cut(s) 317, 334, 446, 758, 868, 884, 1024
Hpy188III TCNNGA 5 cut(s) 48, 86, 206, 861, 911
Hpy8I GTNNAC 4 cut(s) 273, 624, 820, 834
HpyAV CCTTC 5 cut(s) 7, 167, 337, 971, 992
HpyCH4III ACNGT 6 cut(s) 74, 92, 467, 600, 628, 812
HpyCH4IV ACGT 2 cut(s) 223, 548
HpyCH4V TGCA 9 cut(s) 188, 218, 230, 289, 407, 544, 684, 698, 795
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 233
HpyF3I CTNAG 2 cut(s) 331, 558
HpySE526I ACGT 2 cut(s) 223, 548
Hsp92II CATG 6 cut(s) 41, 324, 394, 459, 492, 679
HspAI GCGC 2 cut(s) 108, 324
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 273
Kzo9I GATC 2 cut(s) 469, 863
LmnI GCTCC 1 cut(s) 706
Lsp1109I GCAGC 2 cut(s) 214, 273
LweI GCATC 6 cut(s) 133, 239, 256, 394, 707, 782
MaeI CTAG 3 cut(s) 111, 659, 927
MaeII ACGT 2 cut(s) 223, 548
MaeIII GTNAC 3 cut(s) 16, 568, 668
MalI GATC 2 cut(s) 471, 865
MboI GATC 2 cut(s) 469, 863
MboII GAAGA 9 cut(s) 19, 22, 88, 368, 676, 794, 881, 926, 982
MflI RGATCY 1 cut(s) 863
MhlI GDGCHC 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 5 cut(s) 78, 372, 537, 632, 725
MluNI TGGCCA 1 cut(s) 42
MmeI TCCRAC 2 cut(s) 381, 684
MnlI CCTC 9 cut(s) 110, 328, 353, 420, 461, 519, 736, 868, 927
Mox20I TGGCCA 1 cut(s) 42
Mph1103I ATGCAT 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 981
MscI TGGCCA 1 cut(s) 42
MseI TTAA 4 cut(s) 272, 482, 630, 1030
MslI CAYNNNNRTG 2 cut(s) 31, 183
Msp20I TGGCCA 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 2 cut(s) 224, 233
NdeII GATC 2 cut(s) 469, 863
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 6 cut(s) 41, 324, 394, 459, 492, 679
NlaIV GGNNCC 2 cut(s) 708, 755
NmuCI GTSAC 2 cut(s) 16, 568
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 2 cut(s) 324, 679
OliI CACNNNNGTG 1 cut(s) 31
PaeI GCATGC 1 cut(s) 324
PaeR7I CTCGAG 1 cut(s) 931
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 5 cut(s) 22, 119, 394, 692, 885
PflMI CCANNNNNTGG 2 cut(s) 261, 716
PkrI GCNGC 2 cut(s) 229, 288
Ppu21I YACGTR 1 cut(s) 224
Psp1406I AACGTT 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 708, 755
PspPI GGNCC 2 cut(s) 754, 812
PspXI VCTCGAGB 1 cut(s) 931
PstNI CAGNNNCTG 1 cut(s) 888
PsuI RGATCY 1 cut(s) 863
RsaI GTAC 3 cut(s) 166, 493, 819
RsaNI GTAC 3 cut(s) 165, 492, 818
RseI CAYNNNNRTG 2 cut(s) 31, 183
SaqAI TTAA 4 cut(s) 272, 482, 630, 1030
SatI GCNGC 2 cut(s) 228, 287
Sau3AI GATC 2 cut(s) 469, 863
Sau96I GGNCC 2 cut(s) 754, 812
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 303
SetI ASST 9 cut(s) 18, 55, 187, 226, 412, 515, 551, 833, 932
SfaNI GCATC 6 cut(s) 133, 239, 256, 394, 707, 782
SfcI CTRYAG 2 cut(s) 70, 414
Sfr274I CTCGAG 1 cut(s) 931
SinI GGWCC 2 cut(s) 754, 812
SlaI CTCGAG 1 cut(s) 931
SmiMI CAYNNNNRTG 2 cut(s) 31, 183
SmlI CTYRAG 1 cut(s) 931
SmoI CTYRAG 1 cut(s) 931
SpeI ACTAGT 1 cut(s) 658
SphI GCATGC 1 cut(s) 324
Sse9I AATT 5 cut(s) 78, 372, 537, 632, 725
SsiI CCGC 2 cut(s) 504, 1008
SspI AATATT 2 cut(s) 480, 688
SspMI CTAG 3 cut(s) 111, 659, 927
StyD4I CCNGG 1 cut(s) 433
TaaI ACNGT 6 cut(s) 74, 92, 467, 600, 628, 812
TaiI ACGT 2 cut(s) 226, 551
TaqI TCGA 1 cut(s) 932
TasI AATT 5 cut(s) 78, 372, 537, 632, 725
TatI WGTACW 2 cut(s) 491, 817
TfiI GAWTC 5 cut(s) 22, 119, 394, 692, 885
Tru1I TTAA 4 cut(s) 272, 482, 630, 1030
Tru9I TTAA 4 cut(s) 272, 482, 630, 1030
TscAI CASTG 5 cut(s) 472, 580, 781, 827, 847
TseFI GTSAC 2 cut(s) 16, 568
TseI GCWGC 2 cut(s) 227, 286
Tsp45I GTSAC 2 cut(s) 16, 568
TspDTI ATGAA 3 cut(s) 407, 477, 972
TspRI CASTG 5 cut(s) 472, 580, 781, 827, 847
Van91I CCANNNNNTGG 2 cut(s) 261, 716
VpaK11BI GGWCC 2 cut(s) 754, 812
XapI RAATTY 3 cut(s) 372, 632, 725
XceI RCATGY 2 cut(s) 324, 679
XhoI CTCGAG 1 cut(s) 931
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 3 cut(s) 111, 659, 927
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.