FvH4_1g03990

Serine-threonine kinase receptor-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009140
Physical Location & Seq
Forward (+)
2119886 .. 2123099
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03990.t2

Sequence Viewer

Length: 1074 bp
ATGGAGAAGAAGAAGGTGGCTGTGCCTCTGGTTTGCCACGGCCATTCCCGGCCGGTGGTGGATCTGTTTTACAGTCCGGTCACTCCCGATGGCTTCTTCCTCATCAGTGCCAGCAAGGATTCTAGTCCCATGCTGAGAAATGGTGAGACTGGAGATTGGATTGGAACCTTCGAAGGACATAAGGGTGCAGTCTGGAGCTGCTGTTTGGATACTAATGCCCTAAGGGCTGCCACCGCTTCCGCAGATTTCTCTGCTAAAGTTTGGGATGCGTTAACAGGAGATGTTCTACACTCTTTTGAACATAAGCACATTGTTCGAGCATGTGCGTTTTCAGAGGATACACACCTTTTGTTAACTGGAGGAGTAGAGAAAGTTATCCGTATATACGACTTGAACAGACCAGATGCCCCTCCAAGAGAAGTGGACAAATCCCCTGGTTCAGTTAGAACTGCTGCATGGCTCCACAGTGATCAGACCATATTAAGTTCTTGCACTGATATGGGCGGTGTCAGATTGTGGGATGTAAGAACTGGGAAAATAGTTCAAACACTTGATACCAAGTCATCTGTAACAAGCTCTGAAGTCAGTCAAGATGGTCGATACATCACCACTGCTGATGGGTCTACAGTCAAGTTCTGGGATGCAAATTACTTTGGGTTGGTGAAGAGCTACAACATGCCTTGCACAGTAGAATCAGTGTCCCTGGAACCCAAGTTTGGGAATAAGTTCATTGCTGGTGGAGAAGACATGTGGGTTCATGTTTTCGATTTCCATACTGGTGATGAGATCGCCTGCAACAAGGGTCACCACGGTCCAGTGCACTGCGTTCGATTTTCTCCAGGAGGAGAGTCCTACGCCTCTGGGTCCGAAGACGGCACCATCCGAATATGGCAGACAGGCCCTTTGACTAATGAAGAAAGTGAGGCGCAGGCAGCAAATGGAGCTATCGGAAAGGTGAAGCCACCCGTGGAAGAGGTCTCTCGAAAGATTGAGGGCTTCCAAATCTCCGACGAGGGCAAGTCCAAAGAGAAGGAAGAGGCACCGTCAGCAGCCACAGCATCGGTGAATGTGTGA

Protein Analysis

358

Amino Acids

38.7

Weight (kDa)

5.55

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 16 - 228 4.8e-24 EIF3I
Beta-prop_WDR5 PF25175 17 - 157 2.8e-18 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 17 - 145 2.1e-13 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 18 - 146 2.2e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 28 - 138 1e-08 WDR55
Beta-prop_TEP1_2nd PF25047 30 - 147 9.5e-12 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 31 - 147 3.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 31 - 169 1.1e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 32 - 231 2.6e-33 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 36 - 144 2.7e-06 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 79 - 145 1.2e-08 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 120 - 230 3.3e-09 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 128 - 234 9.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 130 - 233 1e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 161 - 241 1e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 161 - 229 3.5e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 163 - 231 6.2e-12 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 173 - 230 3.2e-10 WDR5 beta-propeller domain
WD40 PF00400 197 - 228 4.6e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 875, 1039
AccI GTMKAC 1 cut(s) 623
AciI CCGC 3 cut(s) 234, 240, 504
AclWI GGATC 1 cut(s) 69
AcoI YGGCCR 2 cut(s) 40, 50
AcuI CTGAAG 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 55, 716, 717
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 3 cut(s) 299, 394, 545
AjnI CCWGG 3 cut(s) 433, 702, 838
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 4 cut(s) 198, 576, 669, 944
AluI AGCT 4 cut(s) 198, 576, 669, 944
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 2 cut(s) 140, 982
Alw44I GTGCAC 1 cut(s) 818
AlwI GGATC 1 cut(s) 69
AoxI GGCC 3 cut(s) 40, 50, 898
ApaLI GTGCAC 1 cut(s) 818
ApeKI GCWGC 5 cut(s) 198, 227, 452, 932, 1049
ArsI GACNNNNNNTTYG 2 cut(s) 886, 918
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 3 cut(s) 812, 864, 899
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 6 cut(s) 155, 598, 673, 791, 797, 967
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 2 cut(s) 812, 864
AxyI CCTNAGG 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 822
BanI GGYRCC 2 cut(s) 875, 1039
BbsI GAAGAC 2 cut(s) 750, 876
Bbv12I GWGCWC 1 cut(s) 822
BbvI GCAGC 5 cut(s) 185, 214, 439, 944, 1061
BccI CCATC 4 cut(s) 83, 587, 611, 887
BceAI ACGGC 2 cut(s) 55, 889
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 809, 843
BciT130I CCWGG 3 cut(s) 435, 704, 840
BciVI GTATCC 2 cut(s) 202, 331
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 140, 982
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 1 cut(s) 624
BfuI GTATCC 2 cut(s) 202, 331
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 5 cut(s) 199, 228, 453, 933, 1050
BlsI GCNGC 5 cut(s) 200, 229, 454, 934, 1051
Bme1390I CCNGG 4 cut(s) 49, 435, 704, 840
Bme18I GGWCC 2 cut(s) 812, 864
BmgT120I GGNCC 3 cut(s) 812, 864, 899
BmiI GGNNCC 6 cut(s) 166, 461, 708, 865, 877, 1041
BmrFI CCNGG 4 cut(s) 49, 435, 704, 840
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 4 cut(s) 256, 394, 631, 1067
BmuI ACTGGG 1 cut(s) 540
BpiI GAAGAC 2 cut(s) 750, 876
BpmI CTGGAG 4 cut(s) 171, 214, 378, 822
Bpu14I TTCGAA 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 49
BsaI GGTCTC 1 cut(s) 982
BsaJI CCNNGG 5 cut(s) 37, 433, 702, 808, 966
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 716, 717
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 5 cut(s) 154, 361, 535, 781, 815
Bse21I CCTNAGG 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 3 cut(s) 435, 704, 840
BseDI CCNNGG 5 cut(s) 37, 433, 702, 808, 966
BseGI GGATG 4 cut(s) 271, 526, 646, 879
BseLI CCNNNNNNNGG 3 cut(s) 55, 716, 717
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 5 cut(s) 154, 361, 535, 781, 815
BseRI GAGGAG 2 cut(s) 375, 858
BseSI GKGCMC 1 cut(s) 822
BseX3I CGGCCG 1 cut(s) 50
BseXI GCAGC 5 cut(s) 185, 214, 439, 944, 1061
BsgI GTGCAG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 3 cut(s) 42, 52, 900
BshNI GGYRCC 2 cut(s) 875, 1039
BsiEI CGRYCG 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 3 cut(s) 49, 53, 77
BslFI GGGAC 2 cut(s) 111, 685
BslI CCNNNNNNNGG 3 cut(s) 55, 716, 717
BsmAI GTCTC 2 cut(s) 140, 982
BsmFI GGGAC 2 cut(s) 111, 685
BsnI GGCC 3 cut(s) 42, 52, 900
Bso31I GGTCTC 1 cut(s) 982
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 3 cut(s) 61, 469, 786
BspACI CCGC 3 cut(s) 234, 240, 504
BspANI GGCC 3 cut(s) 42, 52, 900
BspCNI CTCAG 1 cut(s) 126
BspLI GGNNCC 6 cut(s) 166, 461, 708, 865, 877, 1041
BspPI GGATC 1 cut(s) 69
BspQI GCTCTTC 1 cut(s) 659
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 875, 1039
BspTNI GGTCTC 1 cut(s) 982
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 5 cut(s) 154, 361, 535, 781, 815
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 5 cut(s) 37, 433, 702, 808, 966
BssMI GATC 3 cut(s) 61, 469, 786
Bst2UI CCWGG 3 cut(s) 435, 704, 840
Bst4CI ACNGT 6 cut(s) 74, 467, 628, 688, 812, 1044
Bst6I CTCTTC 3 cut(s) 659, 966, 1029
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 112, 793, 930
BstDEI CTNAG 2 cut(s) 134, 221
BstDSI CCRYGG 3 cut(s) 37, 808, 966
BstEII GGTNACC 1 cut(s) 803
BstF5I GGATG 4 cut(s) 271, 526, 646, 879
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 3 cut(s) 64, 472, 789
BstMAI GTCTC 2 cut(s) 140, 982
BstMBI GATC 3 cut(s) 61, 469, 786
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 6 cut(s) 224, 233, 932, 941, 1046, 1055
BstNI CCWGG 3 cut(s) 435, 704, 840
BstNSI RCATGY 3 cut(s) 324, 679, 751
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 47, 433, 702, 838
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 822
BstV1I GCAGC 5 cut(s) 185, 214, 439, 944, 1061
BstV2I GAAGAC 2 cut(s) 750, 876
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BstZI CGGCCG 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 221
BsuI GTATCC 2 cut(s) 202, 331
BsuRI GGCC 3 cut(s) 42, 52, 900
BtgI CCRYGG 3 cut(s) 37, 808, 966
BtsCI GGATG 4 cut(s) 271, 526, 646, 879
BtsI GCAGTG 2 cut(s) 609, 820
BtsIMutI CAGTG 7 cut(s) 112, 472, 492, 609, 702, 820, 822
Cac8I GCNNGC 3 cut(s) 112, 793, 930
CfoI GCGC 1 cut(s) 928
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 3 cut(s) 812, 864, 899
CspCI CAANNNNNGTGG 2 cut(s) 402, 437
CviAII CATG 6 cut(s) 130, 321, 456, 676, 748, 758
DdeI CTNAG 2 cut(s) 134, 221
DpnI GATC 3 cut(s) 63, 471, 788
DpnII GATC 3 cut(s) 61, 469, 786
EaeI YGGCCR 2 cut(s) 40, 50
EagI CGGCCG 1 cut(s) 50
Eam1104I CTCTTC 3 cut(s) 659, 966, 1029
EarI CTCTTC 3 cut(s) 659, 966, 1029
EclXI CGGCCG 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 982
Eco47I GGWCC 2 cut(s) 812, 864
Eco52I CGGCCG 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 600
Eco81I CCTNAGG 1 cut(s) 221
Eco91I GGTNACC 1 cut(s) 803
EcoO109I RGGNCCY 1 cut(s) 899
EcoO65I GGTNACC 1 cut(s) 803
EcoRII CCWGG 3 cut(s) 433, 702, 838
FaeI CATG 6 cut(s) 133, 324, 459, 679, 751, 761
FaqI GGGAC 2 cut(s) 111, 685
FatI CATG 6 cut(s) 129, 320, 455, 675, 747, 757
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 5 cut(s) 199, 228, 453, 933, 1050
FokI GGATG 4 cut(s) 278, 533, 653, 866
Fsp4HI GCNGC 5 cut(s) 199, 228, 453, 933, 1050
FspBI CTAG 1 cut(s) 123
GlaI GCGC 1 cut(s) 927
GluI GCNGC 5 cut(s) 199, 228, 453, 933, 1050
GsuI CTGGAG 4 cut(s) 171, 214, 378, 822
HaeIII GGCC 3 cut(s) 42, 52, 900
HapII CCGG 3 cut(s) 49, 53, 77
HhaI GCGC 1 cut(s) 928
Hin1II CATG 6 cut(s) 133, 324, 459, 679, 751, 761
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HincII GTYRAC 2 cut(s) 273, 354
HindII GTYRAC 2 cut(s) 273, 354
HinfI GANTC 3 cut(s) 119, 692, 848
HpaI GTTAAC 2 cut(s) 273, 354
HpaII CCGG 3 cut(s) 49, 53, 77
HphI GGTGA 6 cut(s) 155, 598, 673, 791, 797, 967
Hpy166II GTNNAC 5 cut(s) 273, 354, 424, 624, 820
Hpy188I TCNGA 8 cut(s) 334, 474, 512, 580, 868, 884, 950, 1009
Hpy188III TCNNGA 4 cut(s) 86, 193, 590, 981
Hpy8I GTNNAC 5 cut(s) 273, 354, 424, 624, 820
Hpy99I CGWCG 1 cut(s) 1013
HpyAV CCTTC 4 cut(s) 7, 167, 178, 1024
HpyCH4III ACNGT 6 cut(s) 74, 467, 628, 688, 812, 1044
HpyCH4V TGCA 7 cut(s) 188, 455, 492, 644, 684, 795, 820
HpyF10VI GCNNNNNNNGC 6 cut(s) 224, 233, 932, 941, 1046, 1055
HpyF3I CTNAG 2 cut(s) 134, 221
Hsp92II CATG 6 cut(s) 133, 324, 459, 679, 751, 761
HspAI GCGC 1 cut(s) 926
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 2 cut(s) 273, 354
Kzo9I GATC 3 cut(s) 61, 469, 786
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 3 cut(s) 195, 465, 941
Lsp1109I GCAGC 5 cut(s) 185, 214, 439, 944, 1061
LweI GCATC 4 cut(s) 256, 394, 631, 1067
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 3 cut(s) 79, 568, 803
MalI GATC 3 cut(s) 63, 471, 788
MboI GATC 3 cut(s) 61, 469, 786
MboII GAAGA 9 cut(s) 19, 22, 88, 676, 755, 881, 926, 983, 1046
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 1 cut(s) 646
MlyI GAGTC 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 1032
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 3 cut(s) 272, 353, 482
MslI CAYNNNNRTG 3 cut(s) 183, 497, 777
MspI CCGG 3 cut(s) 49, 53, 77
MspR9I CCNGG 4 cut(s) 49, 435, 704, 840
MvaI CCWGG 3 cut(s) 435, 704, 840
MwoI GCNNNNNNNGC 6 cut(s) 224, 233, 932, 941, 1046, 1055
NciI CCSGG 1 cut(s) 49
NdeII GATC 3 cut(s) 61, 469, 786
NlaIII CATG 6 cut(s) 133, 324, 459, 679, 751, 761
NlaIV GGNNCC 6 cut(s) 166, 461, 708, 865, 877, 1041
NmuCI GTSAC 2 cut(s) 79, 803
NspI RCATGY 3 cut(s) 324, 679, 751
NspV TTCGAA 1 cut(s) 171
PciI ACATGT 1 cut(s) 747
PciSI GCTCTTC 1 cut(s) 659
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 2 cut(s) 119, 692
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 5 cut(s) 200, 229, 454, 934, 1051
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
PscI ACATGT 1 cut(s) 747
Psp6I CCWGG 3 cut(s) 433, 702, 838
PspEI GGTNACC 1 cut(s) 803
PspGI CCWGG 3 cut(s) 433, 702, 838
PspN4I GGNNCC 6 cut(s) 166, 461, 708, 865, 877, 1041
PspPI GGNCC 3 cut(s) 812, 864, 899
PsuI RGATCY 1 cut(s) 61
RseI CAYNNNNRTG 3 cut(s) 183, 497, 777
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 3 cut(s) 272, 353, 482
SatI GCNGC 5 cut(s) 199, 228, 453, 933, 1050
Sau3AI GATC 3 cut(s) 61, 469, 786
Sau96I GGNCC 3 cut(s) 812, 864, 899
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 4 cut(s) 49, 435, 704, 840
SduI GDGCHC 1 cut(s) 822
SetI ASST 9 cut(s) 18, 170, 200, 348, 578, 671, 946, 957, 978
SfaNI GCATC 4 cut(s) 256, 394, 631, 1067
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 2 cut(s) 812, 864
SmiMI CAYNNNNRTG 3 cut(s) 183, 497, 777
Sse9I AATT 1 cut(s) 646
SsiI CCGC 3 cut(s) 234, 240, 504
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 4 cut(s) 47, 433, 702, 838
TaaI ACNGT 6 cut(s) 74, 467, 628, 688, 812, 1044
TaqI TCGA 6 cut(s) 171, 316, 598, 765, 829, 982
TasI AATT 1 cut(s) 646
TfiI GAWTC 2 cut(s) 119, 692
Tru1I TTAA 3 cut(s) 272, 353, 482
Tru9I TTAA 3 cut(s) 272, 353, 482
TscAI CASTG 7 cut(s) 112, 472, 499, 616, 702, 822, 827
TseFI GTSAC 2 cut(s) 79, 803
TseI GCWGC 5 cut(s) 198, 227, 452, 932, 1049
Tsp45I GTSAC 2 cut(s) 79, 803
TspDTI ATGAA 3 cut(s) 718, 746, 927
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 7 cut(s) 112, 472, 499, 616, 702, 822, 827
VneI GTGCAC 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 812, 864
XceI RCATGY 3 cut(s) 324, 679, 751
XmiI GTMKAC 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.