AT1G15860

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007865
Physical Location & Seq
Forward (+)
5454766 .. 5457022
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15860.2

Sequence Viewer

Length: 684 bp
ATGCGTCGCTCTTCATCAAAGAAGAAATCAGGTCAATCAACTGAATCAGTCACCACGGATCTCTTTCGCTCAGCTTCGAGCAAGGCCTCGAATAAAGAGATGGATCGAATTGATCACTTATTTAATCAGTATGCCAATAAATCTTCCAGCCTGATTGATCCTGAAGGAATAGAGGAACTATGCTCCAATTTGGAAGTGTCACATACTGATATCAGAATCTTGATGCTTGCTTGGAAGATGAAAGCTGAGAAACAAGGTTACTTTACACATGAGGAGTGGAGAAGAGGCCTCAAGGCTTTAAGAGCTGATACGATCAATAAGTTGAAGAAAGCCCTTCCGGAGCTTGAGAAAGAGGTCAGGAGGCCATCAAATTTTGCAGATTTCTATGCTTATGCCTTCTGTTATTGTTTAACAGAGGAAAAACAGAAGAGCATAGACATAGAGACTATATGTCAACTCCTAGAGATCGTCATGGGATCTACATTTCGAGCCCAAGTTGACTACTTTGTTGAGTATTTAAAGATCCAAAACGACTACAAAGTGATAAACATGGACCAATGGATGGGACTTTACCGGTTCTGTAACGAGATAAGTTTCCCGGACATGGGGGACTATAATCCAGAGCTTGCATGGCCATTGATTCTTGACAATTTTGTTGAGTGGATTCAAGAAAAACAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

227

Amino Acids

26.64

Weight (kDa)

5.57

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 107 - 221 1.8e-27 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 562
AccIII TCCGGA 1 cut(s) 337
AclWI GGATC 5 cut(s) 66, 111, 152, 484, 517
AcoI YGGCCR 1 cut(s) 632
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 183
AfiI CCNNNNNNNGG 3 cut(s) 562, 604, 605
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 2 cut(s) 325, 668
AhdI GACNNNNNGTC 1 cut(s) 450
AluBI AGCT 5 cut(s) 74, 245, 305, 343, 625
AluI AGCT 5 cut(s) 74, 245, 305, 343, 625
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 5 cut(s) 66, 111, 152, 484, 517
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 4 cut(s) 84, 286, 362, 632
ApoI RAATTY 1 cut(s) 370
AsiGI ACCGGT 1 cut(s) 573
AspS9I GGNCC 1 cut(s) 553
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 1 cut(s) 553
BalI TGGCCA 1 cut(s) 634
BanII GRGCYC 1 cut(s) 493
BccI CCATC 3 cut(s) 94, 373, 556
BclI TGATCA 1 cut(s) 112
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 1 cut(s) 461
BlpI GCTNAGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 553
BmeRI GACNNNNNGTC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 553
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 275, 365
BpuMI CCSGG 1 cut(s) 599
BsaJI CCNNGG 1 cut(s) 54
BsaWI WCCGGW 2 cut(s) 337, 573
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 604, 605
Bse118I RCCGGY 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 567
BseLI CCNNNNNNNGG 3 cut(s) 562, 604, 605
BseMII CTCAG 2 cut(s) 84, 237
BseRI GAGGAG 1 cut(s) 287
BshFI GGCC 4 cut(s) 86, 288, 364, 634
BshTI ACCGGT 1 cut(s) 573
BsiSI CCGG 3 cut(s) 338, 574, 599
BslFI GGGAC 2 cut(s) 579, 623
BslI CCNNNNNNNGG 3 cut(s) 562, 604, 605
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 579, 623
BsnI GGCC 4 cut(s) 86, 288, 364, 634
Bsp1286I GDGCHC 1 cut(s) 493
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
Bsp1720I GCTNAGC 1 cut(s) 70
BspANI GGCC 4 cut(s) 86, 288, 364, 634
BspCNI CTCAG 2 cut(s) 83, 238
BspEI TCCGGA 1 cut(s) 337
BspPI GGATC 5 cut(s) 66, 111, 152, 484, 517
BspQI GCTCTTC 2 cut(s) 16, 422
BsrFI RCCGGY 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
Bst6I CTCTTC 3 cut(s) 16, 277, 422
BstC8I GCNNGC 2 cut(s) 228, 627
BstDEI CTNAG 2 cut(s) 70, 246
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 1 cut(s) 567
BstKTI GATC 8 cut(s) 61, 106, 115, 160, 315, 468, 479, 525
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
BstMWI GCNNNNNNNGC 2 cut(s) 302, 631
BstSCI CCNGG 1 cut(s) 597
BstX2I RGATCY 3 cut(s) 58, 476, 522
BstYI RGATCY 3 cut(s) 58, 476, 522
BsuRI GGCC 4 cut(s) 86, 288, 364, 634
BtgI CCRYGG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 567
Cac8I GCNNGC 2 cut(s) 228, 627
Cfr10I RCCGGY 1 cut(s) 573
Cfr13I GGNCC 1 cut(s) 553
CspAI ACCGGT 1 cut(s) 573
CviAII CATG 5 cut(s) 269, 472, 550, 604, 630
DdeI CTNAG 2 cut(s) 70, 246
DpnI GATC 8 cut(s) 60, 105, 114, 159, 314, 467, 478, 524
DpnII GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
DraI TTTAAA 1 cut(s) 519
DriI GACNNNNNGTC 1 cut(s) 450
EaeI YGGCCR 1 cut(s) 632
Eam1104I CTCTTC 3 cut(s) 16, 277, 422
Eam1105I GACNNNNNGTC 1 cut(s) 450
EarI CTCTTC 3 cut(s) 16, 277, 422
Eco147I AGGCCT 2 cut(s) 86, 288
Eco24I GRGCYC 1 cut(s) 493
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 183
EcoRV GATATC 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 5 cut(s) 272, 475, 553, 607, 633
FaqI GGGAC 2 cut(s) 579, 623
FatI CATG 5 cut(s) 268, 471, 549, 603, 629
FbaI TGATCA 1 cut(s) 112
FokI GGATG 1 cut(s) 574
FriOI GRGCYC 1 cut(s) 493
FspBI CTAG 1 cut(s) 461
HaeIII GGCC 4 cut(s) 86, 288, 364, 634
HapII CCGG 3 cut(s) 338, 574, 599
Hin1II CATG 5 cut(s) 272, 475, 553, 607, 633
HincII GTYRAC 2 cut(s) 455, 499
HindII GTYRAC 2 cut(s) 455, 499
HinfI GANTC 4 cut(s) 44, 216, 640, 664
HpaII CCGG 3 cut(s) 338, 574, 599
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 2 cut(s) 455, 499
Hpy188I TCNGA 1 cut(s) 215
Hpy188III TCNNGA 7 cut(s) 161, 220, 338, 358, 620, 644, 668
Hpy8I GTNNAC 2 cut(s) 455, 499
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 3 cut(s) 158, 344, 406
HpyCH4V TGCA 2 cut(s) 377, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 631
HpyF3I CTNAG 2 cut(s) 70, 246
Hsp92II CATG 5 cut(s) 272, 475, 553, 607, 633
Kpn2I TCCGGA 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
LguI GCTCTTC 2 cut(s) 16, 422
LmnI GCTCC 2 cut(s) 188, 340
LpnPI CCDG 9 cut(s) 15, 160, 164, 174, 343, 351, 587, 612, 633
LweI GCATC 1 cut(s) 213
MaeI CTAG 1 cut(s) 461
MaeIII GTNAC 4 cut(s) 49, 198, 257, 581
MalI GATC 8 cut(s) 60, 105, 114, 159, 314, 467, 478, 524
MboI GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
MboII GAAGA 7 cut(s) 3, 34, 135, 247, 294, 337, 439
MflI RGATCY 3 cut(s) 58, 476, 522
MhlI GDGCHC 1 cut(s) 493
MlsI TGGCCA 1 cut(s) 634
MluCI AATT 4 cut(s) 108, 187, 370, 649
MluNI TGGCCA 1 cut(s) 634
MnlI CCTC 8 cut(s) 97, 166, 265, 278, 299, 346, 354, 409
Mox20I TGGCCA 1 cut(s) 634
MroI TCCGGA 1 cut(s) 337
MscI TGGCCA 1 cut(s) 634
MseI TTAA 4 cut(s) 123, 299, 410, 518
Msp20I TGGCCA 1 cut(s) 634
MspI CCGG 3 cut(s) 338, 574, 599
MspR9I CCNGG 1 cut(s) 599
MwoI GCNNNNNNNGC 2 cut(s) 302, 631
NciI CCSGG 1 cut(s) 599
NdeII GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
NlaIII CATG 5 cut(s) 272, 475, 553, 607, 633
NmuCI GTSAC 2 cut(s) 49, 198
PceI AGGCCT 2 cut(s) 86, 288
PciSI GCTCTTC 2 cut(s) 16, 422
PfeI GAWTC 4 cut(s) 44, 216, 640, 664
PflMI CCANNNNNTGG 1 cut(s) 562
PfoI TCCNGGA 1 cut(s) 597
PinAI ACCGGT 1 cut(s) 573
PspPI GGNCC 1 cut(s) 553
PsuI RGATCY 3 cut(s) 58, 476, 522
SapI GCTCTTC 2 cut(s) 16, 422
SaqAI TTAA 4 cut(s) 123, 299, 410, 518
Sau3AI GATC 8 cut(s) 58, 103, 112, 157, 312, 465, 476, 522
Sau96I GGNCC 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 1 cut(s) 493
SetI ASST 8 cut(s) 34, 76, 247, 259, 307, 345, 357, 627
SfaNI GCATC 1 cut(s) 213
SinI GGWCC 1 cut(s) 553
SmlI CTYRAG 2 cut(s) 290, 344
SmoI CTYRAG 2 cut(s) 290, 344
Sse9I AATT 4 cut(s) 108, 187, 370, 649
SseBI AGGCCT 2 cut(s) 86, 288
SspMI CTAG 1 cut(s) 461
StuI AGGCCT 2 cut(s) 86, 288
StyD4I CCNGG 1 cut(s) 597
TaqI TCGA 4 cut(s) 77, 89, 106, 487
TasI AATT 4 cut(s) 108, 187, 370, 649
TfiI GAWTC 4 cut(s) 44, 216, 640, 664
Tru1I TTAA 4 cut(s) 123, 299, 410, 518
Tru9I TTAA 4 cut(s) 123, 299, 410, 518
TseFI GTSAC 2 cut(s) 49, 198
Tsp45I GTSAC 2 cut(s) 49, 198
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 71
Van91I CCANNNNNTGG 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.