Rh2BG370000

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007865
Physical Location & Seq
Reverse (-)
51633361 .. 51638683
5323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG370000.1

Sequence Viewer

Length: 447 bp
ATGAAGGCTGAAAAACAGGGATTCTTTACCCTGGAAGAGTGGCGAGTTGCACTTAAAGCATTAAGGGCGGATAGTTTAAGTAAATTAAAGAAGGCACTTCCAGAGCTAGAGAAAGAGGTCAGGAGGCCATCAAACTTTGTGGATTTCTATTCCTATGCATTCAGATATTGCTTAACAGAGGAGAAACAGAAGAGCATAGATATAGAGAGCATATGCCAATTGTTACATATTGTTTTAGGATCTCAATACCAAGCGCAGGTTGACTCATTTGTAGAGTACTTAAAGACTCAGAATGATTACAAAGTCATAAACATGGATCAATGGATGGGGTTTTACCGATTTTGTGATGAGATAAGTTATCCAGACCTTAGCAACTACGATCCTGAACTTGCATGGCCTTTGATCCTTGACAATTTTGTTGAATGGGTGCGAGAAAAGCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

148

Amino Acids

17.69

Weight (kDa)

5.0

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 28 - 142 1.6e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
AciI CCGC 1 cut(s) 68
AclWI GGATC 4 cut(s) 247, 324, 374, 397
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 2 cut(s) 106, 444
AluI AGCT 2 cut(s) 106, 444
AlwI GGATC 4 cut(s) 247, 324, 374, 397
AoxI GGCC 2 cut(s) 125, 395
AspLEI GCGC 1 cut(s) 256
BccI CCATC 2 cut(s) 136, 319
BciT130I CCWGG 1 cut(s) 32
BfaI CTAG 2 cut(s) 107, 445
BfuAI ACCTGC 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 256
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 194
BshFI GGCC 2 cut(s) 127, 397
BslI CCNNNNNNNGG 1 cut(s) 256
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 2 cut(s) 127, 397
Bsp143I GATC 4 cut(s) 239, 316, 379, 402
BspACI CCGC 1 cut(s) 68
BspANI GGCC 2 cut(s) 127, 397
BspCNI CTCAG 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 4 cut(s) 247, 324, 374, 397
BspQI GCTCTTC 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 239, 316, 379, 402
Bst2UI CCWGG 1 cut(s) 32
Bst6I CTCTTC 2 cut(s) 30, 185
BstDEI CTNAG 2 cut(s) 288, 368
BstF5I GGATG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 242, 319, 382, 405
BstMBI GATC 4 cut(s) 239, 316, 379, 402
BstMWI GCNNNNNNNGC 3 cut(s) 56, 65, 436
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 2 cut(s) 127, 397
BtsCI GGATG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 247
CfoI GCGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 120, 155
CviAII CATG 2 cut(s) 313, 393
CviJI RGCY 5 cut(s) 8, 106, 127, 397, 444
CviKI_1 RGCY 5 cut(s) 8, 106, 127, 397, 444
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 2 cut(s) 288, 368
DpnI GATC 4 cut(s) 241, 318, 381, 404
DpnII GATC 4 cut(s) 239, 316, 379, 402
Eam1104I CTCTTC 2 cut(s) 30, 185
EarI CTCTTC 2 cut(s) 30, 185
EciI GGCGGA 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 160
FaeI CATG 2 cut(s) 316, 396
FaiI YATR 9 cut(s) 156, 197, 203, 212, 214, 228, 308, 314, 394
FatI CATG 2 cut(s) 312, 392
FauNDI CATATG 1 cut(s) 212
FokI GGATG 1 cut(s) 337
FspBI CTAG 2 cut(s) 107, 445
GlaI GCGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 127, 397
HhaI GCGC 1 cut(s) 256
Hin1II CATG 2 cut(s) 316, 396
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 3 cut(s) 21, 263, 286
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 2 cut(s) 164, 291
Hpy188III TCNNGA 4 cut(s) 101, 121, 362, 383
Hpy8I GTNNAC 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 85
HpyCH4V TGCA 3 cut(s) 50, 158, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 65, 436
HpyF3I CTNAG 2 cut(s) 288, 368
Hsp92II CATG 2 cut(s) 316, 396
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 4 cut(s) 239, 316, 379, 402
LguI GCTCTTC 1 cut(s) 185
LpnPI CCDG 8 cut(s) 2, 17, 44, 106, 114, 242, 375, 396
MaeI CTAG 2 cut(s) 107, 445
MaeIII GTNAC 1 cut(s) 222
MalI GATC 4 cut(s) 241, 318, 381, 404
MboI GATC 4 cut(s) 239, 316, 379, 402
MboII GAAGA 2 cut(s) 47, 202
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 1 cut(s) 239
MluCI AATT 3 cut(s) 83, 218, 412
MlyI GAGTC 2 cut(s) 257, 280
MnlI CCTC 3 cut(s) 109, 117, 172
Mph1103I ATGCAT 1 cut(s) 160
MseI TTAA 6 cut(s) 54, 62, 77, 86, 173, 281
MslI CAYNNNNRTG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 218
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 3 cut(s) 56, 65, 436
NdeI CATATG 1 cut(s) 212
NdeII GATC 4 cut(s) 239, 316, 379, 402
NlaIII CATG 2 cut(s) 316, 396
NsiI ATGCAT 1 cut(s) 160
PciSI GCTCTTC 1 cut(s) 185
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 21
PleI GAGTC 2 cut(s) 257, 280
PpsI GAGTC 2 cut(s) 257, 280
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 6 cut(s) 54, 62, 77, 86, 173, 281
Sau3AI GATC 4 cut(s) 239, 316, 379, 402
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 2 cut(s) 257, 280
ScrFI CCNGG 1 cut(s) 32
SetI ASST 5 cut(s) 108, 120, 261, 369, 446
SmiMI CAYNNNNRTG 1 cut(s) 311
Sse9I AATT 3 cut(s) 83, 218, 412
SsiI CCGC 1 cut(s) 68
SspMI CTAG 2 cut(s) 107, 445
StyD4I CCNGG 1 cut(s) 30
TasI AATT 3 cut(s) 83, 218, 412
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 6 cut(s) 54, 62, 77, 86, 173, 281
Tru9I TTAA 6 cut(s) 54, 62, 77, 86, 173, 281
TspDTI ATGAA 1 cut(s) 17
XspI CTAG 2 cut(s) 107, 445
ZrmI AGTACT 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.