RchiOBHm_Chr2g0133421

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007865
Physical Location & Seq
Reverse (-)
50163113 .. 50168405
5293 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50458

Sequence Viewer

Length: 621 bp
ATGAGACGCTCCGCCATTAAAGCAGCTTCTAAAGAGTTGGAACGGATTGATAATGTGTTCTATACCTATGCAAATACAACTTCTGGTTTGATTGACCCAGAGGGAATTGAACGTCTTTGTTCAGATATGGAAGTTGACCCTACAGATGTAAGGGTCTTGATGCTTGCCTGGAAAATGAAGGCTGAAAAACAGGGATTCTTTACCCTGGAAGAGTGGCGAGTTGCACTTAAAGCATTAAGGGCGGATAGTTTAAGTAAATTAAAGAAGGCACTTCCAGAGCTAGAGAAAGAGGTCAGGAGGCCATCAAACTTTGTGGATTTCTATTCCTATGCATTCAGATATTGCTTAACAGAGGAGAAACAGAAGAGCATAGATATAGAGAGCATATGCCAATTGTTACATATTGTTTTAGGATCTCAATACCAAGCGCAGGTTGACTCATTTGTAGAGTACTTAAAGACTCAGAATGATTACAAAGTCATAAACATGGATCAATGGATGGGGTTTTACCGATTTTGTGATGAGATAAGTTATCCAGACCTTAGCAACTACGATCCTGAACTTGCATGGCCTTTGATCCTTGACAATTTTGTTGAATGGGTGCGAGAAAAGCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

206

Amino Acids

24.29

Weight (kDa)

4.97

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 86 - 200 4.4e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 421
AciI CCGC 2 cut(s) 12, 242
AclWI GGATC 4 cut(s) 421, 498, 548, 571
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 2 cut(s) 110, 596
AjnI CCWGG 2 cut(s) 167, 204
AluBI AGCT 3 cut(s) 26, 280, 618
AluI AGCT 3 cut(s) 26, 280, 618
AlwI GGATC 4 cut(s) 421, 498, 548, 571
AoxI GGCC 2 cut(s) 299, 569
ApeKI GCWGC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 430
BbvI GCAGC 1 cut(s) 35
BccI CCATC 2 cut(s) 310, 493
BciT130I CCWGG 2 cut(s) 169, 206
BfaI CTAG 2 cut(s) 281, 619
BfmI CTRYAG 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 2 cut(s) 169, 206
BmrFI CCNGG 2 cut(s) 169, 206
BmsI GCATC 1 cut(s) 150
Bpu10I CCTNAGC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 204
Bsc4I CCNNNNNNNGG 1 cut(s) 430
BseBI CCWGG 2 cut(s) 169, 206
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 476
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 35
BshFI GGCC 2 cut(s) 301, 571
BslI CCNNNNNNNGG 1 cut(s) 430
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 2 cut(s) 301, 571
Bsp143I GATC 4 cut(s) 413, 490, 553, 576
BspACI CCGC 2 cut(s) 12, 242
BspANI GGCC 2 cut(s) 301, 571
BspCNI CTCAG 1 cut(s) 475
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 4 cut(s) 421, 498, 548, 571
BspQI GCTCTTC 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 4 cut(s) 413, 490, 553, 576
Bst2UI CCWGG 2 cut(s) 169, 206
Bst6I CTCTTC 2 cut(s) 204, 359
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 2 cut(s) 462, 542
BstF5I GGATG 1 cut(s) 504
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 4 cut(s) 416, 493, 556, 579
BstMBI GATC 4 cut(s) 413, 490, 553, 576
BstMWI GCNNNNNNNGC 4 cut(s) 20, 230, 239, 610
BstNI CCWGG 2 cut(s) 169, 206
BstSCI CCNGG 2 cut(s) 167, 204
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuRI GGCC 2 cut(s) 301, 571
BtsCI GGATG 1 cut(s) 504
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 165
CfoI GCGC 1 cut(s) 430
CseI GACGC 1 cut(s) 15
Csp6I GTAC 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 294, 329
CviAII CATG 2 cut(s) 487, 567
CviJI RGCY 6 cut(s) 26, 182, 280, 301, 571, 618
CviKI_1 RGCY 6 cut(s) 26, 182, 280, 301, 571, 618
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 2 cut(s) 462, 542
DpnI GATC 4 cut(s) 415, 492, 555, 578
DpnII GATC 4 cut(s) 413, 490, 553, 576
Eam1104I CTCTTC 2 cut(s) 204, 359
EarI CTCTTC 2 cut(s) 204, 359
EciI GGCGGA 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 167, 204
EcoT22I ATGCAT 1 cut(s) 334
FaeI CATG 2 cut(s) 490, 570
FatI CATG 2 cut(s) 486, 566
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 1 cut(s) 511
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 2 cut(s) 281, 619
GlaI GCGC 1 cut(s) 429
GluI GCNGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 301, 571
HgaI GACGC 1 cut(s) 15
HhaI GCGC 1 cut(s) 430
Hin1II CATG 2 cut(s) 490, 570
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 2 cut(s) 136, 436
HindII GTYRAC 2 cut(s) 136, 436
HinfI GANTC 3 cut(s) 195, 437, 460
Hpy166II GTNNAC 2 cut(s) 136, 436
Hpy188I TCNGA 3 cut(s) 124, 338, 465
Hpy188III TCNNGA 5 cut(s) 157, 275, 295, 536, 557
Hpy8I GTNNAC 2 cut(s) 136, 436
HpyAV CCTTC 2 cut(s) 172, 259
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 71, 224, 332, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 230, 239, 610
HpyF3I CTNAG 2 cut(s) 462, 542
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 2 cut(s) 490, 570
HspAI GCGC 1 cut(s) 428
Kzo9I GATC 4 cut(s) 413, 490, 553, 576
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 35
LweI GCATC 1 cut(s) 150
MaeI CTAG 2 cut(s) 281, 619
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 396
MalI GATC 4 cut(s) 415, 492, 555, 578
MboI GATC 4 cut(s) 413, 490, 553, 576
MboII GAAGA 2 cut(s) 221, 376
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 1 cut(s) 413
MluCI AATT 4 cut(s) 105, 257, 392, 586
MlyI GAGTC 2 cut(s) 431, 454
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 4 cut(s) 94, 283, 291, 346
Mph1103I ATGCAT 1 cut(s) 334
MseI TTAA 7 cut(s) 18, 228, 236, 251, 260, 347, 455
MslI CAYNNNNRTG 1 cut(s) 485
MspR9I CCNGG 2 cut(s) 169, 206
MunI CAATTG 1 cut(s) 392
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 2 cut(s) 169, 206
MwoI GCNNNNNNNGC 4 cut(s) 20, 230, 239, 610
NdeI CATATG 1 cut(s) 386
NdeII GATC 4 cut(s) 413, 490, 553, 576
NlaIII CATG 2 cut(s) 490, 570
NsiI ATGCAT 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 359
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 195
PkrI GCNGC 1 cut(s) 25
PleI GAGTC 2 cut(s) 431, 454
PpsI GAGTC 2 cut(s) 431, 454
Psp6I CCWGG 2 cut(s) 167, 204
PspGI CCWGG 2 cut(s) 167, 204
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 485
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 7 cut(s) 18, 228, 236, 251, 260, 347, 455
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 4 cut(s) 413, 490, 553, 576
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 2 cut(s) 431, 454
ScrFI CCNGG 2 cut(s) 169, 206
SetI ASST 8 cut(s) 28, 68, 115, 282, 294, 435, 543, 620
SfaNI GCATC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 485
Sse9I AATT 4 cut(s) 105, 257, 392, 586
SsiI CCGC 2 cut(s) 12, 242
SspMI CTAG 2 cut(s) 281, 619
StyD4I CCNGG 2 cut(s) 167, 204
TaiI ACGT 1 cut(s) 115
TasI AATT 4 cut(s) 105, 257, 392, 586
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 7 cut(s) 18, 228, 236, 251, 260, 347, 455
Tru9I TTAA 7 cut(s) 18, 228, 236, 251, 260, 347, 455
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 1 cut(s) 191
TspGWI ACGGA 1 cut(s) 58
XspI CTAG 2 cut(s) 281, 619
ZrmI AGTACT 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.