Rorug02G0311700

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007865
Physical Location & Seq
Reverse (-)
37518400 .. 37535841
17442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0311700.1

Sequence Viewer

Length: 747 bp
ATGGTTAGGAAAACAGCAGAAAGCAAGTACCCAACTATTCAGCTATCAGAAATACCTGTGAAGCAGTTTGAAAATGATAAACCTGAGCAGATTAAAATTGTCCTCAAGCCTAGGCTTAATAAAACCAATGCGACTGAAAATAGTGGAGCTGGGATGAAAAGAATTCCTGTTAGAAGTGTAGAAGAGAGGAAGGAGGAGTATGATAAGGCGCGTGCTCATATCTTTATCAGTTCTAGCAGTCTTGACTTGAGCGATACATCGTTTCAGGTCCCAACTGATGGTAAAAATGCATCTTTAAGCAAGGATGAGAATGAAAGTTTCAGGAACTCCATGATTGATCCAGAAAAGAATTTAGACATAAATGATCTTTGTACTTCATCTCGAGTTGTCATATTCAGAGATAGGGAAAAAGACCGTACTGACCCAGATTATGATCGCAGTTATGAGAGGTATGTGAAGAGTCTTCCTGCCAATCAGAGGTTTAACGTGACACCTTCCAGTATGCAAAAGTTCCAACCTCAATTTTTGCAGTATAAAGTTGGTTTGTCACAAATGCCAAGGACTTTGCCTTCAATTAGCTATGGGGCACCATCAACCCTAGCCATGAGCCCTTTTTGTGCCATGGGATCGAGTCAGACATCTAGAGAAGCTACATACATTCAGTGGCCAAATGCTGCAATGATGTATGCACATCCGTATGATCAGTTTAGACAAGCTGTTATCCAGTCAGCAGCCCCTGAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

248

Amino Acids

28.26

Weight (kDa)

8.72

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SUZ PF12752 40 - 78 5.9e-07 SUZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 586
AccB7I CCANNNNNTGG 1 cut(s) 278
AccII CGCG 1 cut(s) 211
AclWI GGATC 2 cut(s) 332, 634
AcoI YGGCCR 1 cut(s) 665
AcsI RAATTY 2 cut(s) 162, 349
AfaI GTAC 3 cut(s) 29, 373, 418
AfiI CCNNNNNNNGG 2 cut(s) 278, 477
AgsI TTSAA 2 cut(s) 71, 573
AluBI AGCT 5 cut(s) 43, 149, 579, 650, 716
AluI AGCT 5 cut(s) 43, 149, 579, 650, 716
Alw21I GWGCWC 1 cut(s) 217
AlwI GGATC 2 cut(s) 332, 634
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 1 cut(s) 665
ApeKI GCWGC 2 cut(s) 674, 731
ApoI RAATTY 2 cut(s) 162, 349
AspA2I CCTAGG 1 cut(s) 110
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 1 cut(s) 268
AvrII CCTAGG 1 cut(s) 110
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 589
BalI TGGCCA 1 cut(s) 667
BanI GGYRCC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 455
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 661, 743
BccI CCATC 2 cut(s) 272, 598
BclI TGATCA 1 cut(s) 700
BfaI CTAG 4 cut(s) 111, 234, 599, 642
BisI GCNGC 2 cut(s) 675, 732
BlnI CCTAGG 1 cut(s) 110
BlsI GCNGC 2 cut(s) 676, 733
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 2 cut(s) 270, 588
BmsI GCATC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 2 cut(s) 89, 268
BsaBI GATNNNNATC 1 cut(s) 432
BsaJI CCNNGG 3 cut(s) 110, 557, 621
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 477
Bse1I ACTGG 2 cut(s) 498, 724
Bse21I CCTNAGG 1 cut(s) 738
Bse3DI GCAATG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 110, 557, 621
BseGI GGATG 3 cut(s) 159, 310, 691
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 278, 477
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 2 cut(s) 75, 729
BseNI ACTGG 2 cut(s) 498, 724
BseRI GAGGAG 1 cut(s) 209
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 2 cut(s) 661, 743
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 1 cut(s) 667
BshNI GGYRCC 1 cut(s) 586
BsiHKAI GWGCWC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 278, 477
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 1 cut(s) 667
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 217, 589, 611
Bsp143I GATC 5 cut(s) 337, 364, 433, 626, 700
Bsp19I CCATGG 1 cut(s) 621
BspANI GGCC 1 cut(s) 667
BspCNI CTCAG 2 cut(s) 76, 730
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 270, 588
BspPI GGATC 2 cut(s) 332, 634
BspT107I GGYRCC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 2 cut(s) 498, 724
BssECI CCNNGG 3 cut(s) 110, 557, 621
BssMI GATC 5 cut(s) 337, 364, 433, 626, 700
BssT1I CCWWGG 3 cut(s) 110, 557, 621
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 2 cut(s) 177, 452
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 84, 738
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 159, 310, 691
BstFNI CGCG 1 cut(s) 211
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 5 cut(s) 340, 367, 436, 629, 703
BstMBI GATC 5 cut(s) 337, 364, 433, 626, 700
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 661, 743
BstV2I GAAGAC 1 cut(s) 455
Bsu36I CCTNAGG 1 cut(s) 738
BsuRI GGCC 1 cut(s) 667
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 159, 310, 691
BtsIMutI CAGTG 1 cut(s) 668
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 28, 372, 417
CviAII CATG 3 cut(s) 331, 604, 622
CviQI GTAC 3 cut(s) 28, 372, 417
DdeI CTNAG 2 cut(s) 84, 738
DpnI GATC 5 cut(s) 339, 366, 435, 628, 702
DpnII GATC 5 cut(s) 337, 364, 433, 626, 700
EaeI YGGCCR 1 cut(s) 665
Eam1104I CTCTTC 2 cut(s) 177, 452
EarI CTCTTC 2 cut(s) 177, 452
Eco130I CCWWGG 3 cut(s) 110, 557, 621
Eco24I GRGCYC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 268
Eco81I CCTNAGG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 162
EcoT14I CCWWGG 3 cut(s) 110, 557, 621
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 3 cut(s) 110, 557, 621
FaeI CATG 3 cut(s) 334, 607, 625
FaqI GGGAC 1 cut(s) 254
FatI CATG 3 cut(s) 330, 603, 621
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 2 cut(s) 675, 732
FokI GGATG 3 cut(s) 166, 317, 678
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 2 cut(s) 675, 732
FspBI CTAG 4 cut(s) 111, 234, 599, 642
GlaI GCGC 1 cut(s) 210
GluI GCNGC 2 cut(s) 675, 732
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 1 cut(s) 667
HhaI GCGC 1 cut(s) 211
Hin1II CATG 3 cut(s) 334, 607, 625
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 2 cut(s) 460, 631
Hpy188I TCNGA 4 cut(s) 49, 398, 477, 636
Hpy188III TCNNGA 5 cut(s) 242, 322, 341, 381, 642
HpyAV CCTTC 3 cut(s) 184, 504, 579
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 5 cut(s) 290, 505, 529, 677, 689
HpyF3I CTNAG 2 cut(s) 84, 738
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 3 cut(s) 334, 607, 625
HspAI GCGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 5 cut(s) 337, 364, 433, 626, 700
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 2 cut(s) 661, 743
LweI GCATC 1 cut(s) 299
MaeI CTAG 4 cut(s) 111, 234, 599, 642
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 487, 546
MalI GATC 5 cut(s) 339, 366, 435, 628, 702
MboI GATC 5 cut(s) 337, 364, 433, 626, 700
MboII GAAGA 3 cut(s) 194, 455, 469
MhlI GDGCHC 3 cut(s) 217, 589, 611
MlsI TGGCCA 1 cut(s) 667
MluCI AATT 5 cut(s) 96, 162, 349, 521, 573
MluNI TGGCCA 1 cut(s) 667
MlyI GAGTC 2 cut(s) 469, 640
MmeI TCCRAC 1 cut(s) 538
MnlI CCTC 7 cut(s) 113, 180, 187, 441, 471, 528, 733
Mox20I TGGCCA 1 cut(s) 667
Mph1103I ATGCAT 1 cut(s) 292
MscI TGGCCA 1 cut(s) 667
MseI TTAA 4 cut(s) 93, 117, 296, 483
MslI CAYNNNNRTG 1 cut(s) 696
Msp20I TGGCCA 1 cut(s) 667
MvnI CGCG 1 cut(s) 211
NcoI CCATGG 1 cut(s) 621
NdeII GATC 5 cut(s) 337, 364, 433, 626, 700
NlaIII CATG 3 cut(s) 334, 607, 625
NlaIV GGNNCC 2 cut(s) 270, 588
NmuCI GTSAC 2 cut(s) 487, 546
NsiI ATGCAT 1 cut(s) 292
PaeR7I CTCGAG 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 278
PkrI GCNGC 2 cut(s) 676, 733
PleI GAGTC 2 cut(s) 468, 639
PpsI GAGTC 2 cut(s) 468, 639
PpuMI RGGWCCY 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 270, 588
PspPI GGNCC 1 cut(s) 268
PspPPI RGGWCCY 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 29, 373, 418
RsaNI GTAC 3 cut(s) 28, 372, 417
RseI CAYNNNNRTG 1 cut(s) 696
SaqAI TTAA 4 cut(s) 93, 117, 296, 483
SatI GCNGC 2 cut(s) 675, 732
Sau3AI GATC 5 cut(s) 337, 364, 433, 626, 700
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 2 cut(s) 469, 640
SduI GDGCHC 3 cut(s) 217, 589, 611
SfaNI GCATC 1 cut(s) 299
Sfr274I CTCGAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 3 cut(s) 104, 247, 381
SmoI CTYRAG 3 cut(s) 104, 247, 381
Sse9I AATT 5 cut(s) 96, 162, 349, 521, 573
SspMI CTAG 4 cut(s) 111, 234, 599, 642
StyI CCWWGG 3 cut(s) 110, 557, 621
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 382, 629
TasI AATT 5 cut(s) 96, 162, 349, 521, 573
TatI WGTACW 1 cut(s) 371
Tru1I TTAA 4 cut(s) 93, 117, 296, 483
Tru9I TTAA 4 cut(s) 93, 117, 296, 483
TscAI CASTG 1 cut(s) 668
TseFI GTSAC 2 cut(s) 487, 546
TseI GCWGC 2 cut(s) 674, 731
Tsp45I GTSAC 2 cut(s) 487, 546
TspDTI ATGAA 3 cut(s) 170, 327, 366
TspGWI ACGGA 1 cut(s) 684
TspRI CASTG 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 2 cut(s) 162, 349
XbaI TCTAGA 1 cut(s) 641
XhoI CTCGAG 1 cut(s) 381
XmaJI CCTAGG 1 cut(s) 110
XspI CTAG 4 cut(s) 111, 234, 599, 642
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.