MD17G1220700.v1.1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007865
Physical Location & Seq
Forward (+)
26968710 .. 26973352
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1220700.v1.1.491

Sequence Viewer

Length: 627 bp
ATGAGACGCTCCGCCACTAAAGCAGCTAACAAGGAGTTGGAACGGATTGATGCCGTCTTTAATTCATATGCAAATGCGTCTTCTGGTTTGATTGACCCTGAGGGAATCGAACGTCTTTGTTCAGATTTGGAAGTTGACCATACGGATGTAAGGGTCTTGATGCTTGCCTGGAAAATGAAAGCTGAAAAACAAGGATACTTCACCCTGGAAGAGTGGCGAGTAGGACTTAAAGCACTGAGGGTGGACAATGTAAGTAAATTAAAGAAGGCACTCCCAGAGCTAGAGAAAGAGGTCAGGAGGCCATCAAACTTTGTGGATTTCTATTCCTATGCATTCCGATATTGTTTAACGGAGGAGAAACAGAAGAGCATAGATATAGACAGCATATGCGAGTTATTAAATATTGTTCTAGGATCGCAATACCATTCGCAGGTTGACTTATTTACTCAGTATCTAAAGATTCAGAATGATTACAAAGTCATAAATATGGATCAGTGGATGGGCTTTTACCGGTTTTGCGATGAGATAAGTTTTCCAGACCTTAGCAACTACAACCCTGAACTTGCATGGCCTTTGATCCTCGACAATTTTGTTGAATGGATACGAGAAAAGCAGAGCCAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

209

Amino Acids

24.46

Weight (kDa)

5.13

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 86 - 200 7.1e-29 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 421
AciI CCGC 1 cut(s) 12
AclWI GGATC 3 cut(s) 421, 498, 571
AfiI CCNNNNNNNGG 1 cut(s) 430
AgeI ACCGGT 1 cut(s) 510
AgsI TTSAA 1 cut(s) 596
AjnI CCWGG 2 cut(s) 167, 204
AluBI AGCT 4 cut(s) 26, 182, 280, 624
AluI AGCT 4 cut(s) 26, 182, 280, 624
AlwI GGATC 3 cut(s) 421, 498, 571
AoxI GGCC 2 cut(s) 299, 569
ApeKI GCWGC 1 cut(s) 23
AsiGI ACCGGT 1 cut(s) 510
AsuHPI GGTGA 1 cut(s) 193
AxyI CCTNAGG 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 35
BccI CCATC 2 cut(s) 310, 493
BceAI ACGGC 1 cut(s) 38
BciT130I CCWGG 2 cut(s) 169, 206
BciVI GTATCC 2 cut(s) 188, 594
BfaI CTAG 2 cut(s) 281, 410
BfuAI ACCTGC 1 cut(s) 421
BfuI GTATCC 2 cut(s) 188, 594
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 169, 206
BmrFI CCNGG 2 cut(s) 169, 206
BmsI GCATC 2 cut(s) 40, 150
BpiI GAAGAC 1 cut(s) 72
Bpu10I CCTNAGC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 204
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse118I RCCGGY 1 cut(s) 510
Bse21I CCTNAGG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 169, 206
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 2 cut(s) 151, 504
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 3 cut(s) 90, 227, 461
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 35
BshFI GGCC 2 cut(s) 301, 571
BshTI ACCGGT 1 cut(s) 510
BsiSI CCGG 1 cut(s) 511
BslI CCNNNNNNNGG 1 cut(s) 430
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 2 cut(s) 301, 571
Bsp143I GATC 3 cut(s) 413, 490, 576
BspACI CCGC 1 cut(s) 12
BspANI GGCC 2 cut(s) 301, 571
BspCNI CTCAG 3 cut(s) 91, 228, 460
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 3 cut(s) 421, 498, 571
BspQI GCTCTTC 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 3 cut(s) 413, 490, 576
Bst2UI CCWGG 2 cut(s) 169, 206
Bst6I CTCTTC 2 cut(s) 204, 359
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 4 cut(s) 99, 236, 447, 542
BstF5I GGATG 2 cut(s) 151, 504
BstKTI GATC 3 cut(s) 416, 493, 579
BstMBI GATC 3 cut(s) 413, 490, 576
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 2 cut(s) 169, 206
BstSCI CCNGG 2 cut(s) 167, 204
BstV1I GCAGC 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 72
Bsu36I CCTNAGG 1 cut(s) 99
BsuI GTATCC 2 cut(s) 188, 594
BsuRI GGCC 2 cut(s) 301, 571
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 151, 504
BtsIMutI CAGTG 2 cut(s) 233, 500
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 165
Cfr10I RCCGGY 1 cut(s) 510
CseI GACGC 2 cut(s) 15, 66
CspAI ACCGGT 1 cut(s) 510
CspCI CAANNNNNGTGG 2 cut(s) 294, 329
CviAII CATG 1 cut(s) 567
CviJI RGCY 8 cut(s) 26, 182, 280, 301, 504, 571, 618, 624
CviKI_1 RGCY 8 cut(s) 26, 182, 280, 301, 504, 571, 618, 624
DdeI CTNAG 4 cut(s) 99, 236, 447, 542
DpnI GATC 3 cut(s) 415, 492, 578
DpnII GATC 3 cut(s) 413, 490, 576
Eam1104I CTCTTC 2 cut(s) 204, 359
EarI CTCTTC 2 cut(s) 204, 359
Eco81I CCTNAGG 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 167, 204
EcoT22I ATGCAT 1 cut(s) 334
FaeI CATG 1 cut(s) 570
FatI CATG 1 cut(s) 566
FauNDI CATATG 2 cut(s) 67, 386
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 2 cut(s) 158, 511
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 2 cut(s) 281, 410
GluI GCNGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 301, 571
HapII CCGG 1 cut(s) 511
HgaI GACGC 2 cut(s) 15, 66
Hin1II CATG 1 cut(s) 570
HincII GTYRAC 2 cut(s) 136, 436
HindII GTYRAC 2 cut(s) 136, 436
HinfI GANTC 2 cut(s) 105, 460
HpaII CCGG 1 cut(s) 511
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 136, 244, 436
Hpy188I TCNGA 3 cut(s) 124, 338, 465
Hpy188III TCNNGA 3 cut(s) 157, 295, 536
Hpy8I GTNNAC 3 cut(s) 136, 244, 436
HpyAV CCTTC 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 3 cut(s) 71, 332, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 4 cut(s) 99, 236, 447, 542
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 570
Kzo9I GATC 3 cut(s) 413, 490, 576
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 35
LweI GCATC 2 cut(s) 40, 150
MaeI CTAG 2 cut(s) 281, 410
MaeII ACGT 1 cut(s) 112
MalI GATC 3 cut(s) 415, 492, 578
MboI GATC 3 cut(s) 413, 490, 576
MboII GAAGA 3 cut(s) 72, 221, 376
MluCI AATT 3 cut(s) 61, 257, 586
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 6 cut(s) 94, 231, 283, 291, 346, 590
Mph1103I ATGCAT 1 cut(s) 334
MseI TTAA 5 cut(s) 60, 228, 260, 347, 398
MslI CAYNNNNRTG 2 cut(s) 144, 485
MspI CCGG 1 cut(s) 511
MspR9I CCNGG 2 cut(s) 169, 206
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 2 cut(s) 169, 206
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeI CATATG 2 cut(s) 67, 386
NdeII GATC 3 cut(s) 413, 490, 576
NlaIII CATG 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 359
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 105, 460
PinAI ACCGGT 1 cut(s) 510
PkrI GCNGC 1 cut(s) 25
Psp6I CCWGG 2 cut(s) 167, 204
PspGI CCWGG 2 cut(s) 167, 204
RseI CAYNNNNRTG 2 cut(s) 144, 485
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 5 cut(s) 60, 228, 260, 347, 398
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 3 cut(s) 413, 490, 576
ScrFI CCNGG 2 cut(s) 169, 206
SetI ASST 8 cut(s) 28, 115, 184, 282, 294, 435, 543, 626
SfaNI GCATC 2 cut(s) 40, 150
SmiMI CAYNNNNRTG 2 cut(s) 144, 485
Sse9I AATT 3 cut(s) 61, 257, 586
SsiI CCGC 1 cut(s) 12
SspI AATATT 1 cut(s) 403
SspMI CTAG 2 cut(s) 281, 410
StyD4I CCNGG 2 cut(s) 167, 204
TaiI ACGT 1 cut(s) 115
TaqI TCGA 2 cut(s) 108, 582
TasI AATT 3 cut(s) 61, 257, 586
TfiI GAWTC 2 cut(s) 105, 460
Tru1I TTAA 5 cut(s) 60, 228, 260, 347, 398
Tru9I TTAA 5 cut(s) 60, 228, 260, 347, 398
TscAI CASTG 2 cut(s) 240, 500
TseI GCWGC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 54, 191
TspGWI ACGGA 3 cut(s) 58, 158, 365
TspRI CASTG 2 cut(s) 240, 500
XspI CTAG 2 cut(s) 281, 410
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.