pycom09g14150

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007865
Physical Location & Seq
Forward (+)
13553340 .. 13557279
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g14150.5

Sequence Viewer

Length: 717 bp
ATGTGTTCTTCTAATGCTTTAAATAGTAACCTATTTGAATATTGCTCACACATTTTGTTGAGTTGGCGTTCCATATTATCTGTGCTTAAAACTACTTTAATCTTAATCTGTTCAGCCATTAAAGCAGCTAATAAAGAGTTGGAACGTATTGATGCCATCTTTATCTCATATGAAAATGCATCTTCTGGTTTGATTGACCCAGAGGGAATCGAACGTCTTTGTTCAGATTTGGAAGTTGACCATACCGATGTAAGGGTCTTGATGCTTGCCTGGAAAATGAAAGCTGAAAAACAAGGATACTTTACCTTGGAAGAGTGGCGAGTAGGTCTTAAAGGACTAAGGGTGGACAATGTAAATAAATTAAAGAAGGCACTTCCAGAGCTAGAGAAAGAGGTCGGGAGGCCATCAAACTTTGTGGATTTCTATTCCTATGCATTCGAATATTGTTTAACAGAGGAGAAACAGAAGAGCATAGATATAGACAGCATATGCGAGTTATTAAATATTGTTTTAGGATCTCAATACCATTCGCAGGTTGACTTATTTACTCAGTATCTAAAGATCCAGAGTGATTACAAAGTCATAAATATGGATCAATGGATGGGCTTTTACCGGTTTTGCAATGAGATAAGTTTTCCAGACCTTAGCAACTACGACCCTGAACTTGCATGGCCTTTGATCCTTGACAATTTTGTTGACTGGATACAAGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

239

Amino Acids

27.66

Weight (kDa)

4.76

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AclWI GGATC 4 cut(s) 523, 556, 600, 673
AfiI CCNNNNNNNGG 2 cut(s) 252, 532
AgeI ACCGGT 1 cut(s) 612
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 3 cut(s) 128, 284, 382
AluI AGCT 3 cut(s) 128, 284, 382
AlwI GGATC 4 cut(s) 523, 556, 600, 673
AoxI GGCC 2 cut(s) 401, 671
ApeKI GCWGC 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 612
AsuII TTCGAA 1 cut(s) 438
BbvI GCAGC 1 cut(s) 137
BccI CCATC 3 cut(s) 164, 412, 595
BciT130I CCWGG 1 cut(s) 271
BciVI GTATCC 2 cut(s) 290, 696
BfaI CTAG 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 523
BfuI GTATCC 2 cut(s) 290, 696
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 3 cut(s) 142, 188, 252
Bpu10I CCTNAGC 1 cut(s) 644
Bpu14I TTCGAA 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 612
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 532
Bse118I RCCGGY 1 cut(s) 612
Bse1I ACTGG 1 cut(s) 704
Bse3DI GCAATG 1 cut(s) 628
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 252, 532
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 1 cut(s) 704
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 1 cut(s) 137
BshFI GGCC 2 cut(s) 403, 673
BshTI ACCGGT 1 cut(s) 612
BsiSI CCGG 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 252, 532
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 2 cut(s) 403, 673
Bsp119I TTCGAA 1 cut(s) 438
Bsp143I GATC 4 cut(s) 515, 561, 592, 678
BspANI GGCC 2 cut(s) 403, 673
BspCNI CTCAG 1 cut(s) 562
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 4 cut(s) 523, 556, 600, 673
BspQI GCTCTTC 1 cut(s) 461
BspT104I TTCGAA 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 612
BsrI ACTGG 1 cut(s) 704
BssAI RCCGGY 1 cut(s) 612
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 4 cut(s) 515, 561, 592, 678
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 271
Bst6I CTCTTC 2 cut(s) 306, 461
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 3 cut(s) 338, 549, 644
BstF5I GGATG 1 cut(s) 606
BstKTI GATC 4 cut(s) 518, 564, 595, 681
BstMBI GATC 4 cut(s) 515, 561, 592, 678
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 515, 561
BstYI RGATCY 2 cut(s) 515, 561
BsuI GTATCC 2 cut(s) 290, 696
BsuRI GGCC 2 cut(s) 403, 673
BtsCI GGATG 1 cut(s) 606
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 612
CspAI ACCGGT 1 cut(s) 612
CspCI CAANNNNNGTGG 2 cut(s) 396, 431
CviAII CATG 1 cut(s) 669
CviJI RGCY 7 cut(s) 116, 128, 284, 382, 403, 606, 673
CviKI_1 RGCY 7 cut(s) 116, 128, 284, 382, 403, 606, 673
DdeI CTNAG 3 cut(s) 338, 549, 644
DpnI GATC 4 cut(s) 517, 563, 594, 680
DpnII GATC 4 cut(s) 515, 561, 592, 678
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 306, 461
EarI CTCTTC 2 cut(s) 306, 461
Eco130I CCWWGG 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 2 cut(s) 181, 436
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 672
FatI CATG 1 cut(s) 668
FauNDI CATATG 2 cut(s) 169, 488
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 1 cut(s) 613
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 383
GluI GCNGC 1 cut(s) 126
HaeIII GGCC 2 cut(s) 403, 673
HapII CCGG 1 cut(s) 613
Hin1II CATG 1 cut(s) 672
HincII GTYRAC 3 cut(s) 238, 538, 697
HindII GTYRAC 3 cut(s) 238, 538, 697
HinfI GANTC 1 cut(s) 207
HpaII CCGG 1 cut(s) 613
Hpy166II GTNNAC 4 cut(s) 238, 346, 538, 697
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 5 cut(s) 259, 377, 397, 565, 638
Hpy8I GTNNAC 4 cut(s) 238, 346, 538, 697
HpyAV CCTTC 1 cut(s) 361
HpyCH4IV ACGT 2 cut(s) 145, 214
HpyCH4V TGCA 4 cut(s) 179, 434, 621, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 3 cut(s) 338, 549, 644
HpySE526I ACGT 2 cut(s) 145, 214
Hsp92II CATG 1 cut(s) 672
Kzo9I GATC 4 cut(s) 515, 561, 592, 678
LguI GCTCTTC 1 cut(s) 461
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 3 cut(s) 142, 188, 252
MaeI CTAG 1 cut(s) 383
MaeII ACGT 2 cut(s) 145, 214
MaeIII GTNAC 1 cut(s) 26
MalI GATC 4 cut(s) 517, 563, 594, 680
MboI GATC 4 cut(s) 515, 561, 592, 678
MboII GAAGA 3 cut(s) 174, 323, 478
MflI RGATCY 2 cut(s) 515, 561
MluCI AATT 2 cut(s) 359, 688
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 196, 385, 393, 448
Mph1103I ATGCAT 2 cut(s) 181, 436
MseI TTAA 9 cut(s) 20, 87, 98, 104, 120, 330, 362, 449, 500
MslI CAYNNNNRTG 2 cut(s) 246, 587
MspI CCGG 1 cut(s) 613
MspR9I CCNGG 1 cut(s) 271
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 1 cut(s) 271
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeI CATATG 2 cut(s) 169, 488
NdeII GATC 4 cut(s) 515, 561, 592, 678
NlaIII CATG 1 cut(s) 672
NsiI ATGCAT 2 cut(s) 181, 436
NspV TTCGAA 1 cut(s) 438
PciSI GCTCTTC 1 cut(s) 461
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 207
PinAI ACCGGT 1 cut(s) 612
PkrI GCNGC 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 269
PsuI RGATCY 2 cut(s) 515, 561
RseI CAYNNNNRTG 2 cut(s) 246, 587
SapI GCTCTTC 1 cut(s) 461
SaqAI TTAA 9 cut(s) 20, 87, 98, 104, 120, 330, 362, 449, 500
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 4 cut(s) 515, 561, 592, 678
ScrFI CCNGG 1 cut(s) 271
SfaNI GCATC 3 cut(s) 142, 188, 252
SfuI TTCGAA 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 246, 587
Sse9I AATT 2 cut(s) 359, 688
SspI AATATT 3 cut(s) 41, 443, 505
SspMI CTAG 1 cut(s) 383
StyD4I CCNGG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 2 cut(s) 148, 217
TaqI TCGA 2 cut(s) 210, 438
TasI AATT 2 cut(s) 359, 688
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 9 cut(s) 20, 87, 98, 104, 120, 330, 362, 449, 500
Tru9I TTAA 9 cut(s) 20, 87, 98, 104, 120, 330, 362, 449, 500
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 186, 293
XspI CTAG 1 cut(s) 383
Zsp2I ATGCAT 2 cut(s) 181, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.