Prupe.3G096100_v2.0.a1

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007865
Physical Location & Seq
Forward (+)
7246848 .. 7254494
7647 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G096100.3

Sequence Viewer

Length: 636 bp
ATGAAGAAGATGAGGCGCTCTGCCATTAAAGCAGCTAATAAAGAGTTGGAACGGATTGATGTTTTTTTCAATACATATGCAAATATGTCTTCTGGTTTGATTGACCCAGAGGGAATTGAACGTCTGTGTTCAGACGTGGAAGTTGACCATACTGATGTAAGGATATTGATGCTTGCCTGGAAAATGAAAGCTGAAAAACAGGGATACTTTACCCTGGAAGAGTGGCGAACAGGATTTAAAGCAATAAGGGTGAGCAATAAATATGCGTTAAAGAAGGCACTTCCAGAGCTGGAGAAAGAGGTCAGGAGGCCCACAAACTTTGTGGATTTCTATTCCTATTCATTCCGTTATTGTTTAACAGAGGAGAAACAGAAGAGCATAGATATAGACAGCATATGCGAATTATTAAATATTGTTTTACGATCTCAATACCAAGCGCAGGTTGACTTGTTTGTTCAGTATTTAAAGACTCAGAATGATTACAAAGTCATAAACATGGATCAGTGGATGGGCTTTTACCGGTTCTGCGAAGAGATAAGCTTTCCAGACCTAAGCAACTACGATCCTGAACTTGCATGGCCTTTGATCTTGGACAATTTTGTCGAATGGCTGCGAGAGAAGCAGACCCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

212

Amino Acids

25.19

Weight (kDa)

5.45

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 430
AclWI GGATC 2 cut(s) 507, 557
AfiI CCNNNNNNNGG 1 cut(s) 439
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 2 cut(s) 70, 119
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 176, 213
AluBI AGCT 5 cut(s) 35, 191, 289, 540, 633
AluI AGCT 5 cut(s) 35, 191, 289, 540, 633
AlwI GGATC 2 cut(s) 507, 557
AoxI GGCC 2 cut(s) 308, 578
ApeKI GCWGC 2 cut(s) 32, 610
AsiGI ACCGGT 1 cut(s) 519
AspLEI GCGC 2 cut(s) 18, 439
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 44, 597
BccI CCATC 1 cut(s) 502
BciT130I CCWGG 2 cut(s) 178, 215
BciVI GTATCC 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 430
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 33, 611
BlsI GCNGC 2 cut(s) 34, 612
Bme1390I CCNGG 2 cut(s) 178, 215
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 309
BmrFI CCNGG 2 cut(s) 178, 215
BmsI GCATC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 519
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 519
BseBI CCWGG 2 cut(s) 178, 215
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 44, 597
BshFI GGCC 2 cut(s) 310, 580
BshTI ACCGGT 1 cut(s) 519
BsiSI CCGG 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 439
BsnI GGCC 2 cut(s) 310, 580
Bsp143I GATC 4 cut(s) 422, 499, 562, 585
BspANI GGCC 2 cut(s) 310, 580
BspCNI CTCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 2 cut(s) 507, 557
BspQI GCTCTTC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 4 cut(s) 422, 499, 562, 585
Bst2UI CCWGG 2 cut(s) 178, 215
Bst6I CTCTTC 3 cut(s) 213, 368, 525
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 471, 551
BstF5I GGATG 1 cut(s) 513
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 2 cut(s) 18, 439
BstKTI GATC 4 cut(s) 425, 502, 565, 588
BstMBI GATC 4 cut(s) 422, 499, 562, 585
BstMWI GCNNNNNNNGC 2 cut(s) 29, 619
BstNI CCWGG 2 cut(s) 178, 215
BstSCI CCNGG 2 cut(s) 176, 213
BstV1I GCAGC 2 cut(s) 44, 597
BstV2I GAAGAC 1 cut(s) 81
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 2 cut(s) 310, 580
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 509
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 18, 439
Cfr10I RCCGGY 1 cut(s) 519
Cfr13I GGNCC 1 cut(s) 309
CspAI ACCGGT 1 cut(s) 519
CspCI CAANNNNNGTGG 4 cut(s) 301, 303, 336, 338
CviAII CATG 2 cut(s) 496, 576
CviJI RGCY 9 cut(s) 35, 191, 289, 310, 513, 540, 580, 610, 633
CviKI_1 RGCY 9 cut(s) 35, 191, 289, 310, 513, 540, 580, 610, 633
DdeI CTNAG 2 cut(s) 471, 551
DpnI GATC 4 cut(s) 424, 501, 564, 587
DpnII GATC 4 cut(s) 422, 499, 562, 585
DraI TTTAAA 2 cut(s) 238, 465
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
Eam1104I CTCTTC 3 cut(s) 213, 368, 525
EarI CTCTTC 3 cut(s) 213, 368, 525
EcoRII CCWGG 2 cut(s) 176, 213
FaeI CATG 2 cut(s) 499, 579
FatI CATG 2 cut(s) 495, 575
FauNDI CATATG 2 cut(s) 76, 395
Fnu4HI GCNGC 2 cut(s) 33, 611
FokI GGATG 1 cut(s) 520
Fsp4HI GCNGC 2 cut(s) 33, 611
GlaI GCGC 2 cut(s) 17, 438
GluI GCNGC 2 cut(s) 33, 611
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 2 cut(s) 310, 580
HapII CCGG 1 cut(s) 520
HhaI GCGC 2 cut(s) 18, 439
Hin1II CATG 2 cut(s) 499, 579
Hin6I GCGC 2 cut(s) 16, 437
HinP1I GCGC 2 cut(s) 16, 437
HincII GTYRAC 2 cut(s) 145, 445
HindII GTYRAC 2 cut(s) 145, 445
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 1 cut(s) 469
HpaII CCGG 1 cut(s) 520
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 2 cut(s) 145, 445
Hpy188I TCNGA 2 cut(s) 133, 474
Hpy188III TCNNGA 4 cut(s) 284, 304, 545, 566
Hpy8I GTNNAC 2 cut(s) 145, 445
HpyAV CCTTC 1 cut(s) 268
HpyCH4IV ACGT 2 cut(s) 121, 135
HpyCH4V TGCA 2 cut(s) 80, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 619
HpyF3I CTNAG 2 cut(s) 471, 551
HpySE526I ACGT 2 cut(s) 121, 135
Hsp92II CATG 2 cut(s) 499, 579
HspAI GCGC 2 cut(s) 16, 437
Kzo9I GATC 4 cut(s) 422, 499, 562, 585
LguI GCTCTTC 1 cut(s) 368
Lsp1109I GCAGC 2 cut(s) 44, 597
LweI GCATC 1 cut(s) 159
MaeII ACGT 2 cut(s) 121, 135
MalI GATC 4 cut(s) 424, 501, 564, 587
MboI GATC 4 cut(s) 422, 499, 562, 585
MboII GAAGA 6 cut(s) 16, 19, 81, 230, 385, 542
MluCI AATT 3 cut(s) 114, 401, 595
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 5 cut(s) 6, 103, 292, 300, 355
MseI TTAA 6 cut(s) 27, 237, 269, 356, 407, 464
MslI CAYNNNNRTG 2 cut(s) 153, 494
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 2 cut(s) 178, 215
MvaI CCWGG 2 cut(s) 178, 215
MwoI GCNNNNNNNGC 2 cut(s) 29, 619
NdeI CATATG 2 cut(s) 76, 395
NdeII GATC 4 cut(s) 422, 499, 562, 585
NlaIII CATG 2 cut(s) 499, 579
PciSI GCTCTTC 1 cut(s) 368
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 2 cut(s) 34, 612
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 176, 213
PspGI CCWGG 2 cut(s) 176, 213
PspPI GGNCC 1 cut(s) 309
RseI CAYNNNNRTG 2 cut(s) 153, 494
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 6 cut(s) 27, 237, 269, 356, 407, 464
SatI GCNGC 2 cut(s) 33, 611
Sau3AI GATC 4 cut(s) 422, 499, 562, 585
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 2 cut(s) 178, 215
SfaNI GCATC 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 153, 494
Sse9I AATT 3 cut(s) 114, 401, 595
SspI AATATT 1 cut(s) 412
StyD4I CCNGG 2 cut(s) 176, 213
TaiI ACGT 2 cut(s) 124, 138
TaqI TCGA 1 cut(s) 603
TasI AATT 3 cut(s) 114, 401, 595
Tru1I TTAA 6 cut(s) 27, 237, 269, 356, 407, 464
Tru9I TTAA 6 cut(s) 27, 237, 269, 356, 407, 464
TscAI CASTG 1 cut(s) 509
TseI GCWGC 2 cut(s) 32, 610
TspDTI ATGAA 3 cut(s) 17, 200, 330
TspGWI ACGGA 2 cut(s) 67, 335
TspRI CASTG 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.