Rh2AG364100

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007865
Physical Location & Seq
Reverse (-)
53910149 .. 53915549
5401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG364100.1

Sequence Viewer

Length: 495 bp
ATGGAAGTTGACCCTACAGATGTAAGGGTCTTGATGCTTGCCTGGAAAATGAAGGCTGAAAAACAGGGATTCTTTACCCTGGAAGAGTGGCGAGTTGCACTTAAAGCATTAAGGGCGGATAGTTTAAGTAAATTAAAGAAGGCACTTCCAGAGCTAGAGAAAGAGGTCAGGAGGCCCTCAAACTTTGTGGATTTCTATTCCTATGCATTCAGATATTGCTTAACAGAGGAGAAACAGAAGAGCATAGATATAGAGAGCATATGCCAATTATTACATATTGTTTTAGGATCTCAATACCAAGCGCAGGTTGACTCATTTGTAGAGTACTTAAAGACTCAGAATGATTACAAAGTCATAAACATGGATCAATGGATGGGCTTTTACCGATTTTGTGATGAGATAAGTTATCCAGACCTTAGCAACTACGATCCTGAACTTGCATGGCCTTTGATCCTTGACAATTTTGTTGAATGGGTGCGAGAAAAGCAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

164

Amino Acids

19.58

Weight (kDa)

4.94

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 44 - 158 2.3e-30 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 295
AciI CCGC 1 cut(s) 116
AclWI GGATC 4 cut(s) 295, 372, 422, 445
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 470
AjnI CCWGG 2 cut(s) 41, 78
AluBI AGCT 2 cut(s) 154, 492
AluI AGCT 2 cut(s) 154, 492
AlwI GGATC 4 cut(s) 295, 372, 422, 445
AoxI GGCC 2 cut(s) 173, 443
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 174
BccI CCATC 1 cut(s) 367
BciT130I CCWGG 2 cut(s) 43, 80
BfaI CTAG 2 cut(s) 155, 493
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 2 cut(s) 43, 80
BmgT120I GGNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 43, 80
BmsI GCATC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 304
BseBI CCWGG 2 cut(s) 43, 80
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 350
BseRI GAGGAG 1 cut(s) 242
BshFI GGCC 2 cut(s) 175, 445
BslI CCNNNNNNNGG 1 cut(s) 304
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 2 cut(s) 175, 445
Bsp143I GATC 4 cut(s) 287, 364, 427, 450
BspACI CCGC 1 cut(s) 116
BspANI GGCC 2 cut(s) 175, 445
BspCNI CTCAG 1 cut(s) 349
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 4 cut(s) 295, 372, 422, 445
BspQI GCTCTTC 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 4 cut(s) 287, 364, 427, 450
Bst2UI CCWGG 2 cut(s) 43, 80
Bst6I CTCTTC 2 cut(s) 78, 233
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 336, 416
BstF5I GGATG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 4 cut(s) 290, 367, 430, 453
BstMBI GATC 4 cut(s) 287, 364, 427, 450
BstMWI GCNNNNNNNGC 3 cut(s) 104, 113, 484
BstNI CCWGG 2 cut(s) 43, 80
BstSCI CCNGG 2 cut(s) 41, 78
BstSFI CTRYAG 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuRI GGCC 2 cut(s) 175, 445
BtsCI GGATG 1 cut(s) 378
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 325
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviAII CATG 2 cut(s) 361, 441
CviJI RGCY 6 cut(s) 56, 154, 175, 378, 445, 492
CviKI_1 RGCY 6 cut(s) 56, 154, 175, 378, 445, 492
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 2 cut(s) 336, 416
DpnI GATC 4 cut(s) 289, 366, 429, 452
DpnII GATC 4 cut(s) 287, 364, 427, 450
Eam1104I CTCTTC 2 cut(s) 78, 233
EarI CTCTTC 2 cut(s) 78, 233
EciI GGCGGA 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 174
EcoRII CCWGG 2 cut(s) 41, 78
EcoT22I ATGCAT 1 cut(s) 208
FaeI CATG 2 cut(s) 364, 444
FaiI YATR 9 cut(s) 204, 245, 251, 260, 262, 276, 356, 362, 442
FatI CATG 2 cut(s) 360, 440
FauNDI CATATG 1 cut(s) 260
FokI GGATG 1 cut(s) 385
FspBI CTAG 2 cut(s) 155, 493
GlaI GCGC 1 cut(s) 303
HaeIII GGCC 2 cut(s) 175, 445
HhaI GCGC 1 cut(s) 304
Hin1II CATG 2 cut(s) 364, 444
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 2 cut(s) 10, 310
HindII GTYRAC 2 cut(s) 10, 310
HinfI GANTC 3 cut(s) 69, 311, 334
Hpy166II GTNNAC 2 cut(s) 10, 310
Hpy188I TCNGA 2 cut(s) 212, 339
Hpy188III TCNNGA 5 cut(s) 31, 149, 169, 410, 431
Hpy8I GTNNAC 2 cut(s) 10, 310
HpyAV CCTTC 2 cut(s) 46, 133
HpyCH4V TGCA 3 cut(s) 98, 206, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 113, 484
HpyF3I CTNAG 2 cut(s) 336, 416
Hsp92II CATG 2 cut(s) 364, 444
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 4 cut(s) 287, 364, 427, 450
LguI GCTCTTC 1 cut(s) 233
LweI GCATC 1 cut(s) 24
MaeI CTAG 2 cut(s) 155, 493
MalI GATC 4 cut(s) 289, 366, 429, 452
MboI GATC 4 cut(s) 287, 364, 427, 450
MboII GAAGA 2 cut(s) 95, 250
MflI RGATCY 1 cut(s) 287
MluCI AATT 3 cut(s) 131, 266, 460
MlyI GAGTC 2 cut(s) 305, 328
MnlI CCTC 4 cut(s) 157, 165, 187, 220
Mph1103I ATGCAT 1 cut(s) 208
MseI TTAA 6 cut(s) 102, 110, 125, 134, 221, 329
MslI CAYNNNNRTG 1 cut(s) 359
MspR9I CCNGG 2 cut(s) 43, 80
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 2 cut(s) 43, 80
MwoI GCNNNNNNNGC 3 cut(s) 104, 113, 484
NdeI CATATG 1 cut(s) 260
NdeII GATC 4 cut(s) 287, 364, 427, 450
NlaIII CATG 2 cut(s) 364, 444
NsiI ATGCAT 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 233
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 69
PleI GAGTC 2 cut(s) 305, 328
PpsI GAGTC 2 cut(s) 305, 328
Psp6I CCWGG 2 cut(s) 41, 78
PspGI CCWGG 2 cut(s) 41, 78
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 287
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 6 cut(s) 102, 110, 125, 134, 221, 329
Sau3AI GATC 4 cut(s) 287, 364, 427, 450
Sau96I GGNCC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 2 cut(s) 305, 328
ScrFI CCNGG 2 cut(s) 43, 80
SetI ASST 5 cut(s) 156, 168, 309, 417, 494
SfaNI GCATC 1 cut(s) 24
SfcI CTRYAG 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 359
Sse9I AATT 3 cut(s) 131, 266, 460
SsiI CCGC 1 cut(s) 116
SspMI CTAG 2 cut(s) 155, 493
StyD4I CCNGG 2 cut(s) 41, 78
TasI AATT 3 cut(s) 131, 266, 460
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 6 cut(s) 102, 110, 125, 134, 221, 329
Tru9I TTAA 6 cut(s) 102, 110, 125, 134, 221, 329
TspDTI ATGAA 1 cut(s) 65
XspI CTAG 2 cut(s) 155, 493
ZrmI AGTACT 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.