AT1G16460

sulfurtransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008731
Physical Location & Seq
Reverse (-)
5619545 .. 5622674
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16460.6

Sequence Viewer

Length: 957 bp
ATGGCTTCTTCTGGATCTGAGGCAAAAGCTAATTACGCACCCATATCTACCAATGAACCTGTTGTGTCTGTTGATTGGCTCCACTCTAATCTTGGAGATGCTGATATAAAGGTTTTGGATGCCTCATGGTACATGGCACATGAGCAAAGAAATCCAATCCAAGAGTATCAAGTTGCTCATATTCCAGGTGCTCTCTTCTTTGATTTGAATGGAATAGCAGATCGAAAAACAAATTTACGACACATGCTACCATCTGAGGAAGCTTTTGCTGCTGGTTGTTCTGCTCTTGGAATCGAGAACAACGATGGAGTGGTTGTGTATGATGGAATGGGGCTCTTTAGTGCAGCTCGTGTATGGTGGATGTTTAGAGTCTTTGGACATGACAAAGTATGGGTACTTGATGGAGGCTTGCCGAAATGGCGTGCTTCAGGCTATGACGTTGAATCCAGTGTTTCAAATGATGCCATTTTGAAAGCCAGTGCAGCAACTGAGGCTATAGAGAAGATCTATCAAGGACAAACAATTAGCCCAATAACTTTTCAGACGAAGTTCCGGCCACATCTTGTATTGGCACTTGATCAGGTGAAGGAAAACATCGAGGATAAGACTTATCAGCACATCGATGCGCGTTCAAAGGCTAGATTTGACGGTATTGCTCCAGAACCTTGGAAGGGTTTACCGAGTGGTCATATACCCGGTAGCAAGTGTGTCCCTTTTCCCCTAATGTTTGATTCCTCTCAAACACTGTTACCAGCAGAGGAGCTGAAGAAGCAGTTTGAACAGGAAGACATATCTCTGGACAGTCCGATTGCGGCCTCATGTGGAACCGGTGTAACAGCTTGTATTTTGGCATTGGGACTTTATCGTCTGGGAAAAACTAATGTGGCAATCTATGATGGGTCTTGGACTGAATGGGCAACTGCACCAAACTTGCCCATAGTGGGTTCTTCTTCATAA

Protein Analysis

318

Amino Acids

34.72

Weight (kDa)

5.1

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 23 - 142 1.4e-13 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 864
AccII CGCG 1 cut(s) 628
AciI CCGC 1 cut(s) 812
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 554
AcsI RAATTY 1 cut(s) 232
AcuI CTGAAG 2 cut(s) 411, 785
AfaI GTAC 2 cut(s) 131, 396
AfiI CCNNNNNNNGG 2 cut(s) 671, 941
AgeI ACCGGT 1 cut(s) 827
AgsI TTSAA 6 cut(s) 208, 443, 456, 472, 633, 779
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 5 cut(s) 29, 263, 347, 763, 839
AluI AGCT 5 cut(s) 29, 263, 347, 763, 839
Alw21I GWGCWC 1 cut(s) 193
AlwI GGATC 1 cut(s) 22
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 554, 813
ApeKI GCWGC 3 cut(s) 269, 344, 482
ApoI RAATTY 1 cut(s) 232
AsiGI ACCGGT 1 cut(s) 827
AspLEI GCGC 1 cut(s) 628
AsuC2I CCSGG 1 cut(s) 696
AsuHPI GGTGA 1 cut(s) 595
BanII GRGCYC 1 cut(s) 336
BauI CACGAG 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 3 cut(s) 256, 356, 494
BccI CCATC 5 cut(s) 259, 299, 317, 395, 890
BciT130I CCWGG 1 cut(s) 186
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 696
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 1 cut(s) 495
BglI GCCNNNNNGGC 1 cut(s) 418
BglII AGATCT 1 cut(s) 504
BisI GCNGC 4 cut(s) 270, 345, 483, 813
BlsI GCNGC 4 cut(s) 271, 346, 484, 814
Bme1390I CCNGG 2 cut(s) 186, 696
BmiI GGNNCC 2 cut(s) 80, 826
BmrFI CCNGG 2 cut(s) 186, 696
BmsI GCATC 4 cut(s) 88, 109, 451, 613
BpiI GAAGAC 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 696
Bsa29I ATCGAT 1 cut(s) 621
BsaJI CCNNGG 1 cut(s) 665
BsaWI WCCGGW 1 cut(s) 827
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 671, 941
Bse118I RCCGGY 1 cut(s) 827
Bse1I ACTGG 2 cut(s) 447, 477
BseBI CCWGG 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 2 cut(s) 124, 366
BseLI CCNNNNNNNGG 2 cut(s) 671, 941
BseMII CTCAG 3 cut(s) 9, 246, 480
BseNI ACTGG 2 cut(s) 447, 477
BseRI GAGGAG 1 cut(s) 773
BseXI GCAGC 3 cut(s) 256, 356, 494
BsgI GTGCAG 3 cut(s) 363, 501, 906
Bsh1236I CGCG 1 cut(s) 628
BshFI GGCC 2 cut(s) 556, 815
BshTI ACCGGT 1 cut(s) 827
BshVI ATCGAT 1 cut(s) 621
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 3 cut(s) 553, 696, 828
BslFI GGGAC 2 cut(s) 695, 870
BslI CCNNNNNNNGG 2 cut(s) 671, 941
BsmFI GGGAC 2 cut(s) 695, 870
BsnI GGCC 2 cut(s) 556, 815
Bsp1286I GDGCHC 2 cut(s) 193, 336
Bsp143I GATC 4 cut(s) 14, 220, 504, 577
BspACI CCGC 1 cut(s) 812
BspANI GGCC 2 cut(s) 556, 815
BspCNI CTCAG 3 cut(s) 10, 247, 481
BspDI ATCGAT 1 cut(s) 621
BspFNI CGCG 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 80, 826
BspPI GGATC 1 cut(s) 22
BsrFI RCCGGY 1 cut(s) 827
BsrI ACTGG 2 cut(s) 447, 477
BssAI RCCGGY 1 cut(s) 827
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 4 cut(s) 14, 220, 504, 577
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 665
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 3 cut(s) 650, 747, 803
Bst6I CTCTTC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 410, 423
BstDEI CTNAG 3 cut(s) 18, 255, 489
BstF5I GGATG 2 cut(s) 124, 366
BstFNI CGCG 1 cut(s) 628
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 4 cut(s) 17, 223, 507, 580
BstMBI GATC 4 cut(s) 14, 220, 504, 577
BstMWI GCNNNNNNNGC 6 cut(s) 35, 269, 418, 482, 491, 769
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 2 cut(s) 184, 694
BstSFI CTRYAG 1 cut(s) 495
BstUI CGCG 1 cut(s) 628
BstV1I GCAGC 3 cut(s) 256, 356, 494
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 2 cut(s) 14, 504
BstXI CCANNNNNNTGG 1 cut(s) 666
BstYI RGATCY 2 cut(s) 14, 504
Bsu15I ATCGAT 1 cut(s) 621
BsuRI GGCC 2 cut(s) 556, 815
BsuTUI ATCGAT 1 cut(s) 621
BtsCI GGATG 2 cut(s) 124, 366
BtsIMutI CAGTG 3 cut(s) 454, 484, 743
Cac8I GCNNGC 2 cut(s) 410, 423
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 827
ClaI ATCGAT 1 cut(s) 621
Csp6I GTAC 2 cut(s) 130, 395
CspAI ACCGGT 1 cut(s) 827
CviAII CATG 6 cut(s) 126, 133, 140, 244, 380, 819
CviQI GTAC 2 cut(s) 130, 395
DdeI CTNAG 3 cut(s) 18, 255, 489
DpnI GATC 4 cut(s) 16, 222, 506, 579
DpnII GATC 4 cut(s) 14, 220, 504, 577
DrdI GACNNNNNNGTC 1 cut(s) 864
DseDI GACNNNNNNGTC 1 cut(s) 864
EaeI YGGCCR 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 665
Eco24I GRGCYC 1 cut(s) 336
Eco57I CTGAAG 2 cut(s) 411, 785
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 665
FaeI CATG 6 cut(s) 129, 136, 143, 247, 383, 822
FaqI GGGAC 2 cut(s) 695, 870
FatI CATG 6 cut(s) 125, 132, 139, 243, 379, 818
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 4 cut(s) 270, 345, 483, 813
FokI GGATG 2 cut(s) 131, 373
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 4 cut(s) 270, 345, 483, 813
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 627
GluI GCNGC 4 cut(s) 270, 345, 483, 813
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 2 cut(s) 556, 815
HapII CCGG 3 cut(s) 553, 696, 828
HhaI GCGC 1 cut(s) 628
Hin1II CATG 6 cut(s) 129, 136, 143, 247, 383, 822
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HindIII AAGCTT 1 cut(s) 261
HinfI GANTC 4 cut(s) 291, 369, 443, 731
HpaII CCGG 3 cut(s) 553, 696, 828
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 1 cut(s) 677
Hpy188I TCNGA 4 cut(s) 19, 256, 543, 807
Hpy188III TCNNGA 4 cut(s) 12, 295, 659, 797
Hpy8I GTNNAC 1 cut(s) 677
HpyAV CCTTC 2 cut(s) 580, 664
HpyCH4III ACNGT 3 cut(s) 650, 747, 803
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 3 cut(s) 344, 482, 923
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 269, 418, 482, 491, 769
HpyF3I CTNAG 3 cut(s) 18, 255, 489
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 6 cut(s) 129, 136, 143, 247, 383, 822
HspAI GCGC 1 cut(s) 626
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 14, 220, 504, 577
LmnI GCTCC 3 cut(s) 84, 661, 760
Lsp1109I GCAGC 3 cut(s) 256, 356, 494
LweI GCATC 4 cut(s) 88, 109, 451, 613
MaeI CTAG 1 cut(s) 639
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 747, 832
MalI GATC 4 cut(s) 16, 222, 506, 579
MboI GATC 4 cut(s) 14, 220, 504, 577
MboII GAAGA 6 cut(s) 187, 514, 778, 797, 939, 942
MflI RGATCY 2 cut(s) 14, 504
MhlI GDGCHC 2 cut(s) 193, 336
MluCI AATT 3 cut(s) 31, 232, 522
MlyI GAGTC 1 cut(s) 378
MnlI CCTC 9 cut(s) 13, 133, 250, 398, 484, 592, 745, 751, 826
MslI CAYNNNNRTG 1 cut(s) 621
MspI CCGG 3 cut(s) 553, 696, 828
MspR9I CCNGG 2 cut(s) 186, 696
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 6 cut(s) 35, 269, 418, 482, 491, 769
NciI CCSGG 1 cut(s) 696
NdeII GATC 4 cut(s) 14, 220, 504, 577
NlaIII CATG 6 cut(s) 129, 136, 143, 247, 383, 822
NlaIV GGNNCC 2 cut(s) 80, 826
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 3 cut(s) 291, 443, 731
PinAI ACCGGT 1 cut(s) 827
PkrI GCNGC 4 cut(s) 271, 346, 484, 814
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 2 cut(s) 80, 826
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 2 cut(s) 14, 504
RsaI GTAC 2 cut(s) 131, 396
RsaNI GTAC 2 cut(s) 130, 395
RseI CAYNNNNRTG 1 cut(s) 621
SatI GCNGC 4 cut(s) 270, 345, 483, 813
Sau3AI GATC 4 cut(s) 14, 220, 504, 577
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 2 cut(s) 186, 696
SduI GDGCHC 2 cut(s) 193, 336
SfaNI GCATC 4 cut(s) 88, 109, 451, 613
SfcI CTRYAG 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 621
Sse9I AATT 3 cut(s) 31, 232, 522
SsiI CCGC 1 cut(s) 812
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 2 cut(s) 184, 694
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 3 cut(s) 650, 747, 803
TaiI ACGT 1 cut(s) 441
TaqI TCGA 4 cut(s) 223, 294, 597, 621
TasI AATT 3 cut(s) 31, 232, 522
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 3 cut(s) 291, 443, 731
TscAI CASTG 3 cut(s) 454, 484, 750
TseI GCWGC 3 cut(s) 269, 344, 482
TspDTI ATGAA 2 cut(s) 69, 942
TspRI CASTG 3 cut(s) 454, 484, 750
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.