AT1G17860

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0017494
Physical Location & Seq
Forward (+)
6149178 .. 6150038
861 bp
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UTR
Exon/CDS
Intron
AT1G17860.1

Sequence Viewer

Length: 591 bp
ATGTCATCACTTCTCTATATCTTCCTCCTCTTGGCCGTCTTCATCAGCCACCGTGGAGTCACCACCGAAGCAGCCGTCGAACCAGTTAAGGACATCAATGGAAAATCTCTCCTCACAGGCGTCAACTACTACATCTTGCCTGTAATTCGCGGCCGTGGAGGCGGACTAACCATGTCAAACTTAAAGACCGAAACCTGTCCCACAAGTGTGATCCAAGACCAATTTGAAGTCTCTCAAGGCCTACCGGTTAAATTCTCACCATATGACAAATCAAGAACCATCCCTGTCTCAACTGATGTAAACATCAAATTCTCTCCAACTTCGATCTGGGAATTAGCCAATTTCGACGAGACGACGAAACAATGGTTCATTTCAACTTGTGGCGTTGAAGGGAATCCCGGTCAGAAAACGGTTGATAACTGGTTCAAGATCGACAAATTTGAAAAAGATTACAAGATCAGGTTTTGTCCTACTGTCTGCAATTTCTGCAAAGTCATCTGTAGAGACGTTGGAGTGTTTGTGCAAGATGGAAAGAGAAGACTTGCTTTAAGTGATGTTCCACTAAAGGTTATGTTCAAAAGAGCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

196

Amino Acids

22.08

Weight (kDa)

9.0

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 29 - 194 3.7e-55 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 150
AciI CCGC 2 cut(s) 150, 162
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 2 cut(s) 33, 151
AcsI RAATTY 3 cut(s) 251, 308, 437
AcyI GRCGYC 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 31
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 6 cut(s) 227, 375, 389, 427, 443, 577
AleI CACNNNNGTG 1 cut(s) 206
Alw26I GTCTC 4 cut(s) 235, 292, 344, 498
AlwI GGATC 1 cut(s) 205
AoxI GGCC 3 cut(s) 33, 151, 238
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 3 cut(s) 251, 308, 437
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AsiGI ACCGGT 1 cut(s) 244
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 2 cut(s) 52, 249
BbsI GAAGAC 2 cut(s) 31, 544
BbvI GCAGC 1 cut(s) 83
BccI CCATC 2 cut(s) 287, 521
BceAI ACGGC 3 cut(s) 20, 59, 138
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 4 cut(s) 235, 292, 344, 498
BfaI CTAG 1 cut(s) 589
BfmI CTRYAG 1 cut(s) 499
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 2 cut(s) 72, 151
BlsI GCNGC 2 cut(s) 73, 152
Bme1390I CCNGG 1 cut(s) 399
BmrFI CCNGG 1 cut(s) 399
BpiI GAAGAC 2 cut(s) 31, 544
BpuEI CTTGAG 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 52, 154
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 83, 425
BseDI CCNNGG 2 cut(s) 52, 154
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 31
BseNI ACTGG 2 cut(s) 83, 425
BseRI GAGGAG 2 cut(s) 17, 101
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 3 cut(s) 35, 153, 240
BshTI ACCGGT 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 2 cut(s) 245, 399
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 4 cut(s) 235, 292, 344, 498
BsmBI CGTCTC 2 cut(s) 344, 498
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 3 cut(s) 35, 153, 240
Bsp143I GATC 4 cut(s) 210, 324, 429, 456
BspACI CCGC 2 cut(s) 150, 162
BspANI GGCC 3 cut(s) 35, 153, 240
BspFNI CGCG 1 cut(s) 150
BspPI GGATC 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 2 cut(s) 83, 425
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 52, 154
BssMI GATC 4 cut(s) 210, 324, 429, 456
BssNI GRCGYC 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 53, 412, 475
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 486
BstDSI CCRYGG 2 cut(s) 52, 154
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 4 cut(s) 213, 327, 432, 459
BstMAI GTCTC 4 cut(s) 235, 292, 344, 498
BstMBI GATC 4 cut(s) 210, 324, 429, 456
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 159, 486
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 499
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 2 cut(s) 31, 544
BstZI CGGCCG 1 cut(s) 151
BsuRI GGCC 3 cut(s) 35, 153, 240
BtgI CCRYGG 2 cut(s) 52, 154
BtsCI GGATG 1 cut(s) 279
Cfr10I RCCGGY 1 cut(s) 244
CseI GACGC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 1 cut(s) 172
CviJI RGCY 6 cut(s) 35, 48, 74, 153, 240, 338
CviKI_1 RGCY 6 cut(s) 35, 48, 74, 153, 240, 338
DpnI GATC 4 cut(s) 212, 326, 431, 458
DpnII GATC 4 cut(s) 210, 324, 429, 456
EaeI YGGCCR 2 cut(s) 33, 151
EagI CGGCCG 1 cut(s) 151
EciI GGCGGA 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 240
Eco52I CGGCCG 1 cut(s) 151
Esp3I CGTCTC 2 cut(s) 344, 498
FaeI CATG 1 cut(s) 175
FaiI YATR 6 cut(s) 18, 173, 262, 264, 572, 586
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 1 cut(s) 183
FatI CATG 1 cut(s) 171
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 72, 151
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 72, 151
FspBI CTAG 1 cut(s) 589
GluI GCNGC 2 cut(s) 72, 151
HaeIII GGCC 3 cut(s) 35, 153, 240
HapII CCGG 2 cut(s) 245, 399
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 1 cut(s) 175
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 2 cut(s) 57, 394
HpaII CCGG 2 cut(s) 245, 399
HphI GGTGA 2 cut(s) 52, 249
Hpy166II GTNNAC 2 cut(s) 124, 301
Hpy188I TCNGA 1 cut(s) 405
Hpy188III TCNNGA 2 cut(s) 273, 427
Hpy8I GTNNAC 2 cut(s) 124, 301
Hpy99I CGWCG 3 cut(s) 80, 350, 358
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 3 cut(s) 53, 412, 475
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 3 cut(s) 480, 489, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 486
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 1 cut(s) 175
Kzo9I GATC 4 cut(s) 210, 324, 429, 456
Lsp1109I GCAGC 1 cut(s) 83
MaeI CTAG 1 cut(s) 589
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 1 cut(s) 58
MalI GATC 4 cut(s) 212, 326, 431, 458
MboI GATC 4 cut(s) 210, 324, 429, 456
MboII GAAGA 3 cut(s) 13, 31, 549
MluCI AATT 8 cut(s) 144, 221, 251, 308, 332, 340, 437, 481
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 2 cut(s) 341, 490
MnlI CCTC 4 cut(s) 35, 38, 122, 152
MseI TTAA 4 cut(s) 87, 182, 249, 548
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 2 cut(s) 245, 399
MspR9I CCNGG 1 cut(s) 399
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 159, 486
NciI CCSGG 1 cut(s) 399
NdeI CATATG 1 cut(s) 262
NdeII GATC 4 cut(s) 210, 324, 429, 456
NlaIII CATG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 240
PfeI GAWTC 1 cut(s) 394
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 2 cut(s) 73, 152
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 4 cut(s) 87, 182, 249, 548
SatI GCNGC 2 cut(s) 72, 151
Sau3AI GATC 4 cut(s) 210, 324, 429, 456
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 399
SetI ASST 4 cut(s) 197, 464, 510, 570
SfcI CTRYAG 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 8 cut(s) 144, 221, 251, 308, 332, 340, 437, 481
SseBI AGGCCT 1 cut(s) 240
SsiI CCGC 2 cut(s) 150, 162
SspMI CTAG 1 cut(s) 589
StuI AGGCCT 1 cut(s) 240
StyD4I CCNGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 53, 412, 475
TaiI ACGT 1 cut(s) 510
TaqI TCGA 4 cut(s) 78, 323, 345, 432
TaqII GACCGA 1 cut(s) 203
TasI AATT 8 cut(s) 144, 221, 251, 308, 332, 340, 437, 481
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 4 cut(s) 87, 182, 249, 548
Tru9I TTAA 4 cut(s) 87, 182, 249, 548
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 31, 358
XapI RAATTY 3 cut(s) 251, 308, 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 324
XspI CTAG 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.