Rh7CG002000

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0017494
Physical Location & Seq
Forward (+)
218132 .. 218782
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG002000.1

Sequence Viewer

Length: 651 bp
ATGAGACCATGCGCATTGGCATCACTAGCCTTTATCTGCTACCTCAGTTTCTTTGCCTTCAGAGACAAGGCTTTGTTGGTTGCAGCTGGTGCTGCTCCTGCTCCAGTGCTTGACATCAAAGGTGATAATCTCCGAAGTGGAGTGGAGTATTGGATCCTGCCGGTCATCCGTGGGAGAGGTGGGGGGCTAACTCTAGCCACCGCTGGCAATAGAAATAAAACTAACTGCCCACTTGATGGTGTTCAAGAGCAACACGAGGTATCCAATGGTCTTCCACTCACATTATCGCCTGTGAACTTGAAGAAAGGCGTTGTTCACCTATCCACTGATCTTAACATTAAGTTCTCTACTTCTGCAGGTGTAACAATCTGTCTGGAGACTTCAAAGGTTTGGAAGTTAGACAATTTTAATGAATCAGCAGGGCAATGGTTTGTCACGAGTGGCGGTGTTGAAGGCAACCCGGGTCCTAAAACTATAAGCAACTGGTTTAAGATTGAGAAATATGATGATGACTACAAGCTCGTTTTCTGCCCCACAGTCTGTAATTTCTGCAAGGTTCTATGCAGAGATGTTGACATTTATCTTGAAGATGGGATTAGGCGTCTGGCTCTAAGTGATGTACCAATTAAAGTCATGTTCAAGAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

216

Amino Acids

23.68

Weight (kDa)

8.6

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 36 - 215 5e-55 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 347
Acc16I TGCGCA 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 236
AciI CCGC 2 cut(s) 201, 444
AclWI GGATC 2 cut(s) 148, 161
AcuI CTGAAG 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 6 cut(s) 245, 301, 384, 452, 587, 640
AluBI AGCT 2 cut(s) 86, 520
AluI AGCT 2 cut(s) 86, 520
Alw26I GTCTC 2 cut(s) 57, 371
AlwI GGATC 2 cut(s) 148, 161
Ama87I CYCGRG 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 83, 92
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 464
AsuC2I CCSGG 2 cut(s) 461, 462
AsuHPI GGTGA 2 cut(s) 134, 308
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 1 cut(s) 464
BamHI GGATCC 1 cut(s) 153
BauI CACGAG 2 cut(s) 254, 436
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 79, 95
BccI CCATC 2 cut(s) 230, 584
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 2 cut(s) 461, 462
BcoDI GTCTC 2 cut(s) 57, 371
BfaI CTAG 2 cut(s) 26, 194
BfmI CTRYAG 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 347
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 2 cut(s) 84, 93
BlsI GCNGC 2 cut(s) 85, 94
Bme1390I CCNGG 2 cut(s) 461, 462
Bme18I GGWCC 1 cut(s) 464
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 155, 465
BmrFI CCNGG 2 cut(s) 461, 462
BmsI GCATC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 263
BpmI CTGGAG 2 cut(s) 87, 395
BpuMI CCSGG 2 cut(s) 461, 462
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 2 cut(s) 169, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 104, 488
Bse3DI GCAATG 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 169, 460
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 2 cut(s) 104, 488
BseXI GCAGC 2 cut(s) 79, 95
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 2 cut(s) 161, 461
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 57, 371
BsoBI CYCGRG 1 cut(s) 460
Bsp143I GATC 2 cut(s) 153, 328
BspACI CCGC 2 cut(s) 201, 444
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 155, 465
BspMAI CTGCAG 1 cut(s) 358
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 2 cut(s) 148, 161
BsrDI GCAATG 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 104, 488
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 169, 460
BssMI GATC 2 cut(s) 153, 328
BssNI GRCGYC 1 cut(s) 601
BssSI CACGAG 2 cut(s) 254, 436
Bst2BI CACGAG 2 cut(s) 254, 436
Bst4CI ACNGT 1 cut(s) 538
BstACI GRCGYC 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 44, 611
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 165
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 2 cut(s) 156, 331
BstMAI GTCTC 2 cut(s) 57, 371
BstMBI GATC 2 cut(s) 153, 328
BstMWI GCNNNNNNNGC 4 cut(s) 26, 89, 92, 98
BstSCI CCNGG 2 cut(s) 459, 460
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 79, 95
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuI GTATCC 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 165
BtsIMutI CAGTG 2 cut(s) 111, 324
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 205
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 464
Cfr9I CCCGGG 1 cut(s) 460
CseI GACGC 1 cut(s) 590
Csp6I GTAC 1 cut(s) 620
CviAII CATG 2 cut(s) 9, 634
CviJI RGCY 7 cut(s) 29, 71, 86, 187, 197, 520, 608
CviKI_1 RGCY 7 cut(s) 29, 71, 86, 187, 197, 520, 608
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 2 cut(s) 44, 611
DpnI GATC 2 cut(s) 155, 330
DpnII GATC 2 cut(s) 153, 328
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 464
FaeI CATG 2 cut(s) 12, 637
FaiI YATR 6 cut(s) 10, 476, 504, 562, 635, 649
FatI CATG 2 cut(s) 8, 633
Fnu4HI GCNGC 2 cut(s) 84, 93
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 84, 93
FspAI RTGCGCAY 1 cut(s) 13
FspBI CTAG 2 cut(s) 26, 194
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 84, 93
GsuI CTGGAG 2 cut(s) 87, 395
HapII CCGG 2 cut(s) 161, 461
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 601
Hin1II CATG 2 cut(s) 12, 637
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 1 cut(s) 413
HpaII CCGG 2 cut(s) 161, 461
HphI GGTGA 2 cut(s) 134, 308
Hpy166II GTNNAC 3 cut(s) 295, 316, 574
Hpy188I TCNGA 2 cut(s) 62, 134
Hpy188III TCNNGA 5 cut(s) 245, 374, 436, 584, 640
Hpy8I GTNNAC 3 cut(s) 295, 316, 574
HpyAV CCTTC 2 cut(s) 67, 446
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4V TGCA 4 cut(s) 83, 356, 552, 564
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 89, 92, 98
HpyF3I CTNAG 2 cut(s) 44, 611
Hsp92I GRCGYC 1 cut(s) 601
Hsp92II CATG 2 cut(s) 12, 637
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 153, 328
LmnI GCTCC 2 cut(s) 100, 106
Lsp1109I GCAGC 2 cut(s) 79, 95
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 26, 194
MaeIII GTNAC 2 cut(s) 361, 433
MalI GATC 2 cut(s) 155, 330
MboI GATC 2 cut(s) 153, 328
MboII GAAGA 3 cut(s) 263, 313, 599
MflI RGATCY 1 cut(s) 153
MluCI AATT 3 cut(s) 403, 544, 624
MnlI CCTC 3 cut(s) 53, 170, 250
MseI TTAA 5 cut(s) 333, 339, 408, 489, 627
MspA1I CMGCKG 2 cut(s) 86, 203
MspI CCGG 2 cut(s) 161, 461
MspR9I CCNGG 2 cut(s) 461, 462
MwoI GCNNNNNNNGC 4 cut(s) 26, 89, 92, 98
NciI CCSGG 2 cut(s) 461, 462
NdeII GATC 2 cut(s) 153, 328
NlaIII CATG 2 cut(s) 12, 637
NlaIV GGNNCC 2 cut(s) 155, 465
NmuCI GTSAC 1 cut(s) 433
NsbI TGCGCA 1 cut(s) 13
PaqCI CACCTGC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 413
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 2 cut(s) 85, 94
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
PspN4I GGNNCC 2 cut(s) 155, 465
PspPI GGNCC 1 cut(s) 464
PspPPI RGGWCCY 1 cut(s) 464
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SaqAI TTAA 5 cut(s) 333, 339, 408, 489, 627
SatI GCNGC 2 cut(s) 84, 93
Sau3AI GATC 2 cut(s) 153, 328
Sau96I GGNCC 1 cut(s) 464
ScrFI CCNGG 2 cut(s) 461, 462
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 464
SmaI CCCGGG 1 cut(s) 462
Sse9I AATT 3 cut(s) 403, 544, 624
SsiI CCGC 2 cut(s) 201, 444
SspMI CTAG 2 cut(s) 26, 194
StyD4I CCNGG 2 cut(s) 459, 460
TaaI ACNGT 1 cut(s) 538
TasI AATT 3 cut(s) 403, 544, 624
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 5 cut(s) 333, 339, 408, 489, 627
Tru9I TTAA 5 cut(s) 333, 339, 408, 489, 627
TscAI CASTG 2 cut(s) 111, 331
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 83, 92
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 2 cut(s) 111, 331
Van91I CCANNNNNTGG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 464
XmaI CCCGGG 1 cut(s) 460
XspI CTAG 2 cut(s) 26, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.