Rh7AG002400

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017494
Physical Location & Seq
Forward (+)
213320 .. 213736
417 bp
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UTR
Exon/CDS
Intron
Rh7AG002400.1

Sequence Viewer

Length: 417 bp
ATGTTGTTCAAGAGCAACACGAGGTATCCAATGGTCTTCCACTCACATTATTCGCCTGTGAACTTGAAGAAAGGCGTTGTTCACCTATCCACTGATCTTAACATTAAGTTCTCTACTTCTGCAGCTGTAACAATCTGTCTGGAGACTTCAAAGGTTTGGAAGTTAGACAATTTTGATGAATCAGCAGGGCAATGGTTTGTCACGAGTGGCGGCGTTGAAGGCAACCCGGGTCCTAAAACCATAAGCAACTGGTTTAAGATTGAGAAATATGATGATGACTACAATCTCGTTTTCTGCCCCACAGTCTGTAATTTCTGCAAGGTTCTATGCAGAGATGTTGGCATTTATCTTGAAGATGGGATTAGGCGTCTGGCTCTAAGTGATGTACCAATTAATGTCATGTTCAAGAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

138

Amino Acids

15.68

Weight (kDa)

8.27

Isoelectric Point (pI)

25.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 17 - 137 1.5e-36 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 367
AfaI GTAC 1 cut(s) 387
AgsI TTSAA 6 cut(s) 10, 67, 150, 218, 353, 406
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 122
AseI ATTAAT 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 2 cut(s) 227, 228
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 230
BauI CACGAG 2 cut(s) 19, 202
BbsI GAAGAC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 134
BccI CCATC 1 cut(s) 350
BciVI GTATCC 1 cut(s) 36
BcnI CCSGG 2 cut(s) 227, 228
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 120
BfuI GTATCC 1 cut(s) 36
BisI GCNGC 2 cut(s) 123, 211
BlsI GCNGC 2 cut(s) 124, 212
Bme1390I CCNGG 2 cut(s) 227, 228
Bme18I GGWCC 1 cut(s) 230
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 227, 228
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 161
BpuMI CCSGG 2 cut(s) 227, 228
BsaHI GRCGYC 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 226
BseMI GCAATG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 226
Bsp143I GATC 1 cut(s) 94
BspACI CCGC 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 197
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 94
BssNI GRCGYC 1 cut(s) 367
BssSI CACGAG 2 cut(s) 19, 202
Bst2BI CACGAG 2 cut(s) 19, 202
Bst4CI ACNGT 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 377
BstKTI GATC 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 94
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstSCI CCNGG 2 cut(s) 225, 226
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 134
BstV2I GAAGAC 1 cut(s) 28
BsuI GTATCC 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 230
Cfr9I CCCGGG 1 cut(s) 226
CseI GACGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 386
CviAII CATG 1 cut(s) 400
CviJI RGCY 2 cut(s) 125, 374
CviKI_1 RGCY 2 cut(s) 125, 374
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 1 cut(s) 377
DpnI GATC 1 cut(s) 96
DpnII GATC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 230
FaeI CATG 1 cut(s) 403
FaiI YATR 5 cut(s) 242, 270, 328, 401, 415
FatI CATG 1 cut(s) 399
Fnu4HI GCNGC 2 cut(s) 123, 211
Fsp4HI GCNGC 2 cut(s) 123, 211
GluI GCNGC 2 cut(s) 123, 211
GsuI CTGGAG 1 cut(s) 161
HapII CCGG 1 cut(s) 227
HgaI GACGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 367
Hin1II CATG 1 cut(s) 403
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 227
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 61, 82
Hpy188III TCNNGA 5 cut(s) 10, 140, 202, 350, 406
Hpy8I GTNNAC 2 cut(s) 61, 82
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 3 cut(s) 122, 318, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 367
Hsp92II CATG 1 cut(s) 403
Kzo9I GATC 1 cut(s) 94
LpnPI CCDG 6 cut(s) 69, 125, 171, 235, 240, 356
Lsp1109I GCAGC 1 cut(s) 134
MaeIII GTNAC 2 cut(s) 127, 199
MalI GATC 1 cut(s) 96
MboI GATC 1 cut(s) 94
MboII GAAGA 3 cut(s) 28, 79, 365
MluCI AATT 3 cut(s) 169, 310, 390
MnlI CCTC 1 cut(s) 15
MseI TTAA 4 cut(s) 99, 105, 255, 393
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 227, 228
MwoI GCNNNNNNNGC 1 cut(s) 219
NciI CCSGG 2 cut(s) 227, 228
NdeII GATC 1 cut(s) 94
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 2 cut(s) 124, 212
PpuMI RGGWCCY 1 cut(s) 230
PshBI ATTAAT 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 230
PspPPI RGGWCCY 1 cut(s) 230
PstI CTGCAG 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SaqAI TTAA 4 cut(s) 99, 105, 255, 393
SatI GCNGC 2 cut(s) 123, 211
Sau3AI GATC 1 cut(s) 94
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 227, 228
SetI ASST 5 cut(s) 26, 87, 127, 156, 324
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 1 cut(s) 230
SmaI CCCGGG 1 cut(s) 228
Sse9I AATT 3 cut(s) 169, 310, 390
SsiI CCGC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 225, 226
TaaI ACNGT 1 cut(s) 304
TasI AATT 3 cut(s) 169, 310, 390
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 99, 105, 255, 393
Tru9I TTAA 4 cut(s) 99, 105, 255, 393
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 192
TspMI CCCGGG 1 cut(s) 226
TspRI CASTG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 230
VspI ATTAAT 1 cut(s) 393
XmaI CCCGGG 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.