Rw7G000190

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0017494
Physical Location & Seq
Forward (+)
204254 .. 204902
649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000190.1

Sequence Viewer

Length: 600 bp
ATGAGACCCTGCGCATTGGCATCACTAGCCTTTATCTGCTACCTCGGTTTCTCTGCCTTCAGAGACAAGGCTTTGTTGGTTGCAGCTGATGCTGCTCCTGCTCCAGTGCTTGACATCAAAGATGATAATCTCCGAAGTGGCGTGGAGTATTGGATCCTGCCGGTCATCCGTGGGAGAGGTGGGGGGCTAACCCTAGCCACCGCTGGCAATAGAAATAAAACTAACTGCCCACTTGATGTTGTTCGAGAGCAACACGAGGTATCCAATGGTCTTCCACTCACATTATTCGCCTGTGATCTTGAAGAAAGGCCTGTAACAATCTGTCTGGAGACTTCAAAGGTTTGGAAGTTAGACAATTTTGATGAATCAGCAGGGCAATGGTTTGTCACGAGTGGCGGCGTTGAAGGCAACCCGGGTCCTAAAACCATAAGCAACTGGTTTAAGATTGAGAAATATGATGATGACTACAAGCTCGTTTCTGCCCCAGTCTGTAATTTCTGCAAGGTTCTATGCAGAGATGTTGGCATTTATCTTGAAGATGGGATTAGGCGTCTGGCTCTAAGTGATGTACCAATTAATGTCATGTTCAAGAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

199

Amino Acids

21.97

Weight (kDa)

5.52

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 36 - 198 5.3e-42 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 13
AciI CCGC 2 cut(s) 201, 396
AclWI GGATC 2 cut(s) 148, 161
AcuI CTGAAG 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 550
AfaI GTAC 1 cut(s) 570
AgsI TTSAA 5 cut(s) 302, 336, 404, 536, 589
AluBI AGCT 2 cut(s) 86, 472
AluI AGCT 2 cut(s) 86, 472
Alw26I GTCTC 2 cut(s) 57, 323
AlwI GGATC 2 cut(s) 148, 161
Ama87I CYCGRG 1 cut(s) 412
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 83, 92
AseI ATTAAT 1 cut(s) 576
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 2 cut(s) 413, 414
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 1 cut(s) 416
BamHI GGATCC 1 cut(s) 153
BauI CACGAG 2 cut(s) 254, 388
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 79, 95
BccI CCATC 1 cut(s) 533
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 2 cut(s) 413, 414
BcoDI GTCTC 2 cut(s) 57, 323
BfaI CTAG 2 cut(s) 26, 194
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 3 cut(s) 84, 93, 397
BlsI GCNGC 3 cut(s) 85, 94, 398
Bme1390I CCNGG 2 cut(s) 413, 414
Bme18I GGWCC 1 cut(s) 416
BmeT110I CYCGRG 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 155, 417
BmrFI CCNGG 2 cut(s) 413, 414
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 29, 79
BmuI ACTGGG 1 cut(s) 479
BpiI GAAGAC 1 cut(s) 263
BpmI CTGGAG 2 cut(s) 87, 347
BpuMI CCSGG 2 cut(s) 413, 414
BsaBI GATNNNNATC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 43, 169, 412
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 3 cut(s) 104, 440, 485
Bse3DI GCAATG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 43, 169, 412
BseGI GGATG 1 cut(s) 165
BseJI GATNNNNATC 1 cut(s) 126
BseMI GCAATG 1 cut(s) 383
BseNI ACTGG 3 cut(s) 104, 440, 485
BseXI GCAGC 2 cut(s) 79, 95
BshFI GGCC 1 cut(s) 310
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 2 cut(s) 161, 413
BsmAI GTCTC 2 cut(s) 57, 323
BsnI GGCC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 412
Bsp143I GATC 2 cut(s) 153, 295
BspACI CCGC 2 cut(s) 201, 396
BspANI GGCC 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 155, 417
BspPI GGATC 2 cut(s) 148, 161
BsrDI GCAATG 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 3 cut(s) 104, 440, 485
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 3 cut(s) 43, 169, 412
BssMI GATC 2 cut(s) 153, 295
BssNI GRCGYC 1 cut(s) 550
BssSI CACGAG 2 cut(s) 254, 388
Bst2BI CACGAG 2 cut(s) 254, 388
BstACI GRCGYC 1 cut(s) 550
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 165
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 2 cut(s) 156, 298
BstMAI GTCTC 2 cut(s) 57, 323
BstMBI GATC 2 cut(s) 153, 295
BstMWI GCNNNNNNNGC 5 cut(s) 26, 89, 92, 98, 405
BstSCI CCNGG 2 cut(s) 411, 412
BstV1I GCAGC 2 cut(s) 79, 95
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 205
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 416
Cfr9I CCCGGG 1 cut(s) 412
CseI GACGC 1 cut(s) 539
Csp6I GTAC 1 cut(s) 569
CviAII CATG 1 cut(s) 583
CviJI RGCY 8 cut(s) 29, 71, 86, 187, 197, 310, 472, 557
CviKI_1 RGCY 8 cut(s) 29, 71, 86, 187, 197, 310, 472, 557
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 1 cut(s) 560
DpnI GATC 2 cut(s) 155, 297
DpnII GATC 2 cut(s) 153, 295
Eco147I AGGCCT 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 416
FaeI CATG 1 cut(s) 586
FaiI YATR 5 cut(s) 428, 456, 511, 584, 598
FatI CATG 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 84, 93, 397
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 84, 93, 397
FspBI CTAG 2 cut(s) 26, 194
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 1 cut(s) 13
GluI GCNGC 3 cut(s) 84, 93, 397
GsuI CTGGAG 2 cut(s) 87, 347
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 161, 413
HgaI GACGC 1 cut(s) 539
HhaI GCGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 550
Hin1II CATG 1 cut(s) 586
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 1 cut(s) 365
HpaII CCGG 2 cut(s) 161, 413
Hpy188I TCNGA 2 cut(s) 62, 134
Hpy188III TCNNGA 6 cut(s) 245, 299, 326, 388, 533, 589
HpyAV CCTTC 2 cut(s) 67, 398
HpyCH4V TGCA 3 cut(s) 83, 501, 513
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 89, 92, 98, 405
HpyF3I CTNAG 1 cut(s) 560
Hsp92I GRCGYC 1 cut(s) 550
Hsp92II CATG 1 cut(s) 586
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 153, 295
LmnI GCTCC 2 cut(s) 100, 106
Lsp1109I GCAGC 2 cut(s) 79, 95
LweI GCATC 2 cut(s) 29, 79
MaeI CTAG 2 cut(s) 26, 194
MaeIII GTNAC 2 cut(s) 313, 385
MalI GATC 2 cut(s) 155, 297
MboI GATC 2 cut(s) 153, 295
MboII GAAGA 3 cut(s) 263, 314, 548
MflI RGATCY 1 cut(s) 153
MluCI AATT 3 cut(s) 355, 493, 573
MnlI CCTC 3 cut(s) 53, 170, 250
MseI TTAA 2 cut(s) 441, 576
MspA1I CMGCKG 2 cut(s) 86, 203
MspI CCGG 2 cut(s) 161, 413
MspR9I CCNGG 2 cut(s) 413, 414
MwoI GCNNNNNNNGC 5 cut(s) 26, 89, 92, 98, 405
NciI CCSGG 2 cut(s) 413, 414
NdeII GATC 2 cut(s) 153, 295
NlaIII CATG 1 cut(s) 586
NlaIV GGNNCC 2 cut(s) 155, 417
NmuCI GTSAC 1 cut(s) 385
NsbI TGCGCA 1 cut(s) 13
PceI AGGCCT 1 cut(s) 310
PfeI GAWTC 1 cut(s) 365
PkrI GCNGC 3 cut(s) 85, 94, 398
PpuMI RGGWCCY 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 416
PspN4I GGNNCC 2 cut(s) 155, 417
PspPI GGNCC 1 cut(s) 416
PspPPI RGGWCCY 1 cut(s) 416
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
SaqAI TTAA 2 cut(s) 441, 576
SatI GCNGC 3 cut(s) 84, 93, 397
Sau3AI GATC 2 cut(s) 153, 295
Sau96I GGNCC 1 cut(s) 416
ScrFI CCNGG 2 cut(s) 413, 414
SetI ASST 7 cut(s) 45, 88, 181, 261, 342, 474, 507
SfaNI GCATC 2 cut(s) 29, 79
SinI GGWCC 1 cut(s) 416
SmaI CCCGGG 1 cut(s) 414
Sse9I AATT 3 cut(s) 355, 493, 573
SseBI AGGCCT 1 cut(s) 310
SsiI CCGC 2 cut(s) 201, 396
SspMI CTAG 2 cut(s) 26, 194
StuI AGGCCT 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 411, 412
TaqI TCGA 1 cut(s) 244
TasI AATT 3 cut(s) 355, 493, 573
TauI GCSGC 1 cut(s) 399
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 441, 576
Tru9I TTAA 2 cut(s) 441, 576
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 2 cut(s) 83, 92
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 378
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 412
TspRI CASTG 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 416
VspI ATTAAT 1 cut(s) 576
XmaI CCCGGG 1 cut(s) 412
XspI CTAG 2 cut(s) 26, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.