RLG00000005564

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017494
Physical Location & Seq
Reverse (-)
68561025 .. 68561675
651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005564

Sequence Viewer

Length: 651 bp
ATGAGACCCTGCGCATTGGCATCACTAGCCTTTATCTGCTACCTCAGTTTCTTTGCCTTCAGAGACAAGGCTTTGTTGGTTGCAGCTGATGCTGCTCCTGCTGCAGTGCTTGACATCAAAGGTGATAATCTCCTAAGTGGAGTGGAGTATTGGATCCTGCCGGTCATCCGTGGGAGAGGTGGGGGGCTAACTCTAGCCACCGCTGGCAATAGAAATAAAACTAACTGCCCACTTGATGTTGTTCAAGAGCAACACGAGGTATCCAATGGTCTTCCACTCACATTTTCGCCTGTGAACTTGAAGAAAGGCGTTGTTCACCCATCCACTGATCTTAACATTAAGTTCTCTAGTTCTGCAGCTGTAACAATCTGTTTGGAGACTTCAAAGGTTTGGAAGTTAGACAATTTTGATGAATCAGCCGGGCAATGGTTTGTCACGAGTGGCGGCGTTGAAGGCAACCCGGGTCCTGAAACTATAAGCAACTGGTTTAAGATTGAGACATATGATGATGACTACAAGCTCGTTTTCTGCCCCAGAGTCTGTAATTTCTGCAAGGTTCTATGCAGAGATGTTGGCATTTATCTTGAAGTTGGGATTAGGCGTCTGGCTCTAAGTGATGTACCAATTAAAGTCATGTTCAAGAGAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

217

Amino Acids

23.68

Weight (kDa)

7.51

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 36 - 215 2.7e-57 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 13
AciI CCGC 2 cut(s) 201, 444
AclWI GGATC 2 cut(s) 148, 161
AcuI CTGAAG 1 cut(s) 43
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 1 cut(s) 426
AgsI TTSAA 6 cut(s) 245, 301, 384, 452, 587, 640
AluBI AGCT 3 cut(s) 86, 359, 520
AluI AGCT 3 cut(s) 86, 359, 520
Alw26I GTCTC 3 cut(s) 57, 371, 491
AlwI GGATC 2 cut(s) 148, 161
AlwNI CAGNNNCTG 1 cut(s) 540
Ama87I CYCGRG 1 cut(s) 460
ApeKI GCWGC 4 cut(s) 83, 92, 101, 356
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 464
AsuC2I CCSGG 3 cut(s) 421, 461, 462
AsuHPI GGTGA 2 cut(s) 134, 308
AvaI CYCGRG 1 cut(s) 460
AvaII GGWCC 1 cut(s) 464
BamHI GGATCC 1 cut(s) 153
BauI CACGAG 2 cut(s) 254, 436
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 4 cut(s) 79, 88, 95, 368
BccI CCATC 1 cut(s) 328
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 3 cut(s) 421, 461, 462
BcoDI GTCTC 3 cut(s) 57, 371, 491
BfaI CTAG 3 cut(s) 26, 194, 348
BfmI CTRYAG 2 cut(s) 102, 354
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 5 cut(s) 84, 93, 102, 357, 445
BlsI GCNGC 5 cut(s) 85, 94, 103, 358, 446
Bme1390I CCNGG 3 cut(s) 421, 461, 462
Bme18I GGWCC 1 cut(s) 464
BmeT110I CYCGRG 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 155, 465
BmrFI CCNGG 3 cut(s) 421, 461, 462
BmsI GCATC 2 cut(s) 29, 79
BpiI GAAGAC 1 cut(s) 263
BpuMI CCSGG 3 cut(s) 421, 461, 462
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 2 cut(s) 169, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 426
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 431
BseDI CCNNGG 2 cut(s) 169, 460
BseGI GGATG 2 cut(s) 165, 320
BseLI CCNNNNNNNGG 1 cut(s) 426
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 488
BseXI GCAGC 4 cut(s) 79, 88, 95, 368
BsiHKCI CYCGRG 1 cut(s) 460
BsiSI CCGG 3 cut(s) 161, 420, 461
BslI CCNNNNNNNGG 1 cut(s) 426
BsmAI GTCTC 3 cut(s) 57, 371, 491
BsoBI CYCGRG 1 cut(s) 460
Bsp143I GATC 2 cut(s) 153, 328
BspACI CCGC 2 cut(s) 201, 444
BspCNI CTCAG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 155, 465
BspMAI CTGCAG 2 cut(s) 106, 358
BspPI GGATC 2 cut(s) 148, 161
BsrDI GCAATG 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 1 cut(s) 488
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 2 cut(s) 169, 460
BssMI GATC 2 cut(s) 153, 328
BssNI GRCGYC 1 cut(s) 601
BssSI CACGAG 2 cut(s) 254, 436
Bst2BI CACGAG 2 cut(s) 254, 436
BstACI GRCGYC 1 cut(s) 601
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 3 cut(s) 44, 134, 611
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 2 cut(s) 165, 320
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 2 cut(s) 156, 331
BstMAI GTCTC 3 cut(s) 57, 371, 491
BstMBI GATC 2 cut(s) 153, 328
BstMWI GCNNNNNNNGC 6 cut(s) 26, 89, 92, 98, 101, 453
BstSCI CCNGG 3 cut(s) 419, 459, 460
BstSFI CTRYAG 2 cut(s) 102, 354
BstV1I GCAGC 4 cut(s) 79, 88, 95, 368
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuI GTATCC 1 cut(s) 271
BtgI CCRYGG 1 cut(s) 169
BtsCI GGATG 2 cut(s) 165, 320
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 324
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 540
CfoI GCGC 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 464
Cfr9I CCCGGG 1 cut(s) 460
CseI GACGC 1 cut(s) 590
Csp6I GTAC 1 cut(s) 620
CviAII CATG 1 cut(s) 634
CviJI RGCY 9 cut(s) 29, 71, 86, 187, 197, 359, 419, 520, 608
CviKI_1 RGCY 9 cut(s) 29, 71, 86, 187, 197, 359, 419, 520, 608
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 3 cut(s) 44, 134, 611
DpnI GATC 2 cut(s) 155, 330
DpnII GATC 2 cut(s) 153, 328
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 464
FaeI CATG 1 cut(s) 637
FaiI YATR 6 cut(s) 476, 502, 504, 562, 635, 649
FatI CATG 1 cut(s) 633
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 5 cut(s) 84, 93, 102, 357, 445
FokI GGATG 2 cut(s) 152, 307
Fsp4HI GCNGC 5 cut(s) 84, 93, 102, 357, 445
FspBI CTAG 3 cut(s) 26, 194, 348
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 1 cut(s) 13
GluI GCNGC 5 cut(s) 84, 93, 102, 357, 445
HapII CCGG 3 cut(s) 161, 420, 461
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 601
Hin1II CATG 1 cut(s) 637
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 2 cut(s) 413, 537
HpaII CCGG 3 cut(s) 161, 420, 461
HphI GGTGA 2 cut(s) 134, 308
Hpy166II GTNNAC 2 cut(s) 295, 316
Hpy188I TCNGA 1 cut(s) 62
Hpy188III TCNNGA 5 cut(s) 245, 436, 467, 584, 640
Hpy8I GTNNAC 2 cut(s) 295, 316
HpyAV CCTTC 2 cut(s) 67, 446
HpyCH4V TGCA 5 cut(s) 83, 104, 356, 552, 564
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 89, 92, 98, 101, 453
HpyF3I CTNAG 3 cut(s) 44, 134, 611
Hsp92I GRCGYC 1 cut(s) 601
Hsp92II CATG 1 cut(s) 637
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 2 cut(s) 153, 328
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 4 cut(s) 79, 88, 95, 368
LweI GCATC 2 cut(s) 29, 79
MaeI CTAG 3 cut(s) 26, 194, 348
MaeIII GTNAC 2 cut(s) 361, 433
MalI GATC 2 cut(s) 155, 330
MboI GATC 2 cut(s) 153, 328
MboII GAAGA 2 cut(s) 263, 313
MflI RGATCY 1 cut(s) 153
MluCI AATT 3 cut(s) 403, 544, 624
MlyI GAGTC 1 cut(s) 546
MnlI CCTC 3 cut(s) 53, 170, 250
MseI TTAA 4 cut(s) 333, 339, 489, 627
MspA1I CMGCKG 3 cut(s) 86, 203, 359
MspI CCGG 3 cut(s) 161, 420, 461
MspR9I CCNGG 3 cut(s) 421, 461, 462
MwoI GCNNNNNNNGC 6 cut(s) 26, 89, 92, 98, 101, 453
NciI CCSGG 3 cut(s) 421, 461, 462
NdeI CATATG 1 cut(s) 502
NdeII GATC 2 cut(s) 153, 328
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 2 cut(s) 155, 465
NmuCI GTSAC 1 cut(s) 433
NsbI TGCGCA 1 cut(s) 13
PfeI GAWTC 1 cut(s) 413
PkrI GCNGC 5 cut(s) 85, 94, 103, 358, 446
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
PspN4I GGNNCC 2 cut(s) 155, 465
PspPI GGNCC 1 cut(s) 464
PspPPI RGGWCCY 1 cut(s) 464
PstI CTGCAG 2 cut(s) 106, 358
PstNI CAGNNNCTG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 2 cut(s) 86, 359
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 333, 339, 489, 627
SatI GCNGC 5 cut(s) 84, 93, 102, 357, 445
Sau3AI GATC 2 cut(s) 153, 328
Sau96I GGNCC 1 cut(s) 464
SchI GAGTC 1 cut(s) 546
ScrFI CCNGG 3 cut(s) 421, 461, 462
SetI ASST 9 cut(s) 45, 88, 124, 181, 261, 361, 390, 522, 558
SfaNI GCATC 2 cut(s) 29, 79
SfcI CTRYAG 2 cut(s) 102, 354
SinI GGWCC 1 cut(s) 464
SmaI CCCGGG 1 cut(s) 462
Sse9I AATT 3 cut(s) 403, 544, 624
SsiI CCGC 2 cut(s) 201, 444
SspMI CTAG 3 cut(s) 26, 194, 348
StyD4I CCNGG 3 cut(s) 419, 459, 460
TasI AATT 3 cut(s) 403, 544, 624
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 4 cut(s) 333, 339, 489, 627
Tru9I TTAA 4 cut(s) 333, 339, 489, 627
TscAI CASTG 2 cut(s) 111, 331
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 4 cut(s) 83, 92, 101, 356
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 1 cut(s) 426
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 2 cut(s) 111, 331
VpaK11BI GGWCC 1 cut(s) 464
XmaI CCCGGG 1 cut(s) 460
XspI CTAG 3 cut(s) 26, 194, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.