Prupe.5G074400_v2.0.a1

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0017494
Physical Location & Seq
Reverse (-)
8860374 .. 8861503
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G074400.1

Sequence Viewer

Length: 615 bp
ATGTCATTGAGGCTAGCACTCTGCTTCCTTCTCCTTGCCTTCTCTGCCAAGCCATGGTCAGTTGCAGCCGATGCTGCTCCGTCCCCGGTGCTTGACATCACCGGCAAGAAGCTCCGAACGGGCACCGATTATTACATCTTACCAGTCATCCGTGGGAGGGGCGGGGGGCTCACTCTGGCTAGCACTAGCAACACAACCAGCTGCCCACTTGATGTTGTTCAAGAGCAGCATGAGGTGTCGAATGGTCTCCCGCTGACATTTTCACCGGTGAACCCTAAGAAAGGCGTGGTTCGGGTCTCAACCGATCATAACATCAAGTTCTCTGCTGCCACAATCTGCGTTCAATCCACGATGTGGAAATTGGACAAGTTTGATGAGCAAACAGGACAGTGGTTTGTCACGAGTGGTGGGGTTGAGGGCAATCCCGGTCGTCAAACCACAAGCAATTGGTTTAAGATTGAGAAATATGATGAAGACTACAAGCTCGTCTTCTGCCCAACAGTTTGCAATTTCTGCAAAGTGATATGCAGAGATGTTGGGATTTTTATTCAAGATGGGAAAAGGCGCCTGGCTCTCACTGATGTGCCATTCAAAGTCATGTTCAAGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

205

Amino Acids

22.53

Weight (kDa)

9.08

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 122, 564
AccB7I CCANNNNNTGG 2 cut(s) 54, 354
AciI CCGC 2 cut(s) 162, 251
AcyI GRCGYC 1 cut(s) 565
AdeI CACNNNGTG 1 cut(s) 354
AfiI CCNNNNNNNGG 4 cut(s) 54, 157, 281, 354
AgeI ACCGGT 1 cut(s) 265
AgsI TTSAA 5 cut(s) 221, 344, 551, 592, 604
AjnI CCWGG 1 cut(s) 567
AleI CACNNNNGTG 1 cut(s) 581
AluBI AGCT 4 cut(s) 112, 201, 484, 611
AluI AGCT 4 cut(s) 112, 201, 484, 611
Alw26I GTCTC 2 cut(s) 251, 301
ApeKI GCWGC 5 cut(s) 65, 74, 201, 226, 326
AsiGI ACCGGT 1 cut(s) 265
AspLEI GCGC 1 cut(s) 567
AsuC2I CCSGG 2 cut(s) 86, 426
AsuHPI GGTGA 3 cut(s) 91, 255, 280
AsuNHI GCTAGC 2 cut(s) 13, 179
BaeGI GKGCMC 1 cut(s) 125
BanI GGYRCC 2 cut(s) 122, 564
BanII GRGCYC 1 cut(s) 171
BauI CACGAG 1 cut(s) 400
BbsI GAAGAC 2 cut(s) 480, 481
BbvI GCAGC 5 cut(s) 61, 77, 188, 238, 313
BccI CCATC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 569
BcnI CCSGG 2 cut(s) 86, 426
BcoDI GTCTC 2 cut(s) 251, 301
BfaI CTAG 3 cut(s) 14, 180, 186
BfoI RGCGCY 1 cut(s) 568
BisI GCNGC 5 cut(s) 66, 75, 202, 227, 327
BlsI GCNGC 5 cut(s) 67, 76, 203, 228, 328
Bme1390I CCNGG 3 cut(s) 86, 426, 569
BmiI GGNNCC 2 cut(s) 124, 566
BmrFI CCNGG 3 cut(s) 86, 426, 569
BmsI GCATC 1 cut(s) 61
BmtI GCTAGC 2 cut(s) 17, 183
BpiI GAAGAC 2 cut(s) 480, 481
BpuMI CCSGG 2 cut(s) 86, 426
BsaHI GRCGYC 1 cut(s) 565
BsaI GGTCTC 2 cut(s) 251, 301
BsaJI CCNNGG 3 cut(s) 53, 84, 151
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 157, 281, 354
Bse118I RCCGGY 2 cut(s) 101, 265
Bse1I ACTGG 1 cut(s) 143
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 3 cut(s) 53, 84, 151
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 4 cut(s) 54, 157, 281, 354
BseNI ACTGG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 5 cut(s) 61, 77, 188, 238, 313
Bsh1285I CGRYCG 1 cut(s) 430
BshNI GGYRCC 2 cut(s) 122, 564
BshTI ACCGGT 1 cut(s) 265
BsiEI CGRYCG 1 cut(s) 430
BsiSI CCGG 4 cut(s) 86, 102, 266, 426
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 4 cut(s) 54, 157, 281, 354
BsmAI GTCTC 2 cut(s) 251, 301
BsmFI GGGAC 1 cut(s) 67
Bso31I GGTCTC 2 cut(s) 251, 301
Bsp1286I GDGCHC 2 cut(s) 125, 171
Bsp143I GATC 1 cut(s) 304
Bsp19I CCATGG 1 cut(s) 53
BspACI CCGC 2 cut(s) 162, 251
BspLI GGNNCC 2 cut(s) 124, 566
BspOI GCTAGC 2 cut(s) 17, 183
BspT107I GGYRCC 2 cut(s) 122, 564
BspTNI GGTCTC 2 cut(s) 251, 301
BsrFI RCCGGY 2 cut(s) 101, 265
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 2 cut(s) 101, 265
BssECI CCNNGG 3 cut(s) 53, 84, 151
BssMI GATC 1 cut(s) 304
BssNI GRCGYC 1 cut(s) 565
BssSI CACGAG 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 53
Bst2BI CACGAG 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 390, 502
BstACI GRCGYC 1 cut(s) 565
BstAPI GCANNNNNTGC 2 cut(s) 71, 513
BstC8I GCNNGC 2 cut(s) 15, 181
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 2 cut(s) 53, 151
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 2 cut(s) 251, 301
BstMBI GATC 1 cut(s) 304
BstMCI CGRYCG 1 cut(s) 430
BstMWI GCNNNNNNNGC 4 cut(s) 44, 71, 74, 513
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 3 cut(s) 84, 424, 567
BstSLI GKGCMC 1 cut(s) 125
BstV1I GCAGC 5 cut(s) 61, 77, 188, 238, 313
BstV2I GAAGAC 2 cut(s) 480, 481
BtgI CCRYGG 2 cut(s) 53, 151
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 395, 576
Cac8I GCNNGC 2 cut(s) 15, 181
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 2 cut(s) 101, 265
CspAI ACCGGT 1 cut(s) 265
CviAII CATG 3 cut(s) 54, 230, 598
DdeI CTNAG 1 cut(s) 276
DinI GGCGCC 1 cut(s) 566
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
DraIII CACNNNGTG 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 251, 301
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 171
EgeI GGCGCC 1 cut(s) 566
EheI GGCGCC 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 3 cut(s) 57, 233, 601
FaiI YATR 6 cut(s) 55, 231, 309, 468, 526, 599
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 1 cut(s) 67
FatI CATG 3 cut(s) 53, 229, 597
FauI CCCGC 2 cut(s) 155, 258
Fnu4HI GCNGC 5 cut(s) 66, 75, 202, 227, 327
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 5 cut(s) 66, 75, 202, 227, 327
FspBI CTAG 3 cut(s) 14, 180, 186
GlaI GCGC 1 cut(s) 566
GluI GCNGC 5 cut(s) 66, 75, 202, 227, 327
HaeII RGCGCY 1 cut(s) 568
HapII CCGG 4 cut(s) 86, 102, 266, 426
HhaI GCGC 1 cut(s) 567
Hin1I GRCGYC 1 cut(s) 565
Hin1II CATG 3 cut(s) 57, 233, 601
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 609
HpaII CCGG 4 cut(s) 86, 102, 266, 426
HphI GGTGA 3 cut(s) 91, 255, 280
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 4 cut(s) 221, 400, 551, 604
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 38, 49
HpyCH4III ACNGT 2 cut(s) 390, 502
HpyCH4V TGCA 4 cut(s) 65, 507, 516, 528
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 71, 74, 513
HpyF3I CTNAG 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 565
Hsp92II CATG 3 cut(s) 57, 233, 601
HspAI GCGC 1 cut(s) 565
KasI GGCGCC 1 cut(s) 564
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 2 cut(s) 82, 117
Lsp1109I GCAGC 5 cut(s) 61, 77, 188, 238, 313
LweI GCATC 1 cut(s) 61
MaeI CTAG 3 cut(s) 14, 180, 186
MaeIII GTNAC 1 cut(s) 397
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MboII GAAGA 2 cut(s) 481, 485
MfeI CAATTG 1 cut(s) 445
MhlI GDGCHC 2 cut(s) 125, 171
MluCI AATT 3 cut(s) 359, 445, 508
Mly113I GGCGCC 1 cut(s) 565
MnlI CCTC 4 cut(s) 3, 150, 226, 409
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 581
MspA1I CMGCKG 2 cut(s) 201, 253
MspI CCGG 4 cut(s) 86, 102, 266, 426
MspR9I CCNGG 3 cut(s) 86, 426, 569
MunI CAATTG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 4 cut(s) 44, 71, 74, 513
NarI GGCGCC 1 cut(s) 565
NciI CCSGG 2 cut(s) 86, 426
NcoI CCATGG 1 cut(s) 53
NdeII GATC 1 cut(s) 304
NheI GCTAGC 2 cut(s) 13, 179
NlaIII CATG 3 cut(s) 57, 233, 601
NlaIV GGNNCC 2 cut(s) 124, 566
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 581
PflMI CCANNNNNTGG 2 cut(s) 54, 354
PinAI ACCGGT 1 cut(s) 265
PkrI GCNGC 5 cut(s) 67, 76, 203, 228, 328
PluTI GGCGCC 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 2 cut(s) 124, 566
PvuII CAGCTG 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 581
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 5 cut(s) 66, 75, 202, 227, 327
Sau3AI GATC 1 cut(s) 304
ScrFI CCNGG 3 cut(s) 86, 426, 569
SduI GDGCHC 2 cut(s) 125, 171
SetI ASST 5 cut(s) 114, 203, 237, 486, 613
SfaNI GCATC 1 cut(s) 61
SfoI GGCGCC 1 cut(s) 566
SgrAI CRCCGGYG 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 581
Sse9I AATT 3 cut(s) 359, 445, 508
SsiI CCGC 2 cut(s) 162, 251
SspDI GGCGCC 1 cut(s) 564
SspMI CTAG 3 cut(s) 14, 180, 186
StyD4I CCNGG 3 cut(s) 84, 424, 567
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 2 cut(s) 390, 502
TaqI TCGA 1 cut(s) 239
TasI AATT 3 cut(s) 359, 445, 508
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 395, 583
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 5 cut(s) 65, 74, 201, 226, 326
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 2 cut(s) 69, 140
TspRI CASTG 2 cut(s) 395, 583
Van91I CCANNNNNTGG 2 cut(s) 54, 354
XagI CCTNNNNNAGG 1 cut(s) 279
XspI CTAG 3 cut(s) 14, 180, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.