FvH4_5g18500

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017494
Physical Location & Seq
Reverse (-)
10649647 .. 10650366
720 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18500.t1

Sequence Viewer

Length: 657 bp
ATGAGGTTCTGCACATTGGCATCACAAGTCCTTATCTGCTACCTCAGTCTCCTTGCCTTCAGAGGAAAGCCATTGTTGGTTGCAGTCCATGCTGATTCTGATCCCGCTCCAGTGCTTGACATCAAAGGTCATAATATCCAAACTGGGGTTGAGTATTGGATCTTACCTGTCATTCGTGGGAGAGGTGGGGGGCTAACACTAGCCACCACTGGCAATAGAAATAGAAGCATCTGCCCACTTGATGTTGTTCAAGAGCAACAAGAGGTATCTAATGGTCTTCCACTCACATTTTCGCCTGTGAATCTGAAGATAGGTGTCGTTCGCCTATCCACTGATCTCAATATCAAGTTCTCTACATCTGCAGCTGTAACTATCTGTCTGAACACTTCGAAGGTTTGGAAGTTAGACAACCTTGATGAATCAACAGGGCAACGGTTTGTCACGACTGGTGGCGTAGAAGGCAATCCCGGCCCTAAAACTATAAGCAATTGGTTCAAGATTGAGAAATATGATAAGGACTACAAGCTCGTCTTTTGCCCCACAGTATGTAATTTCTGCAAGGTTATATGCGGTGATGTTGGCATTTATCTTGAAGGCGGGGTCAGGCGTCTGGTTCTAAGTAAAGTACCACTCAAAGTCATGTTCAAGAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

219

Amino Acids

23.95

Weight (kDa)

9.23

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 38 - 217 2.2e-60 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 107
AciI CCGC 3 cut(s) 105, 570, 597
AclWI GGATC 2 cut(s) 95, 167
AcuI CTGAAG 2 cut(s) 43, 326
AcyI GRCGYC 1 cut(s) 607
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 4 cut(s) 251, 496, 593, 646
AluBI AGCT 2 cut(s) 365, 526
AluI AGCT 2 cut(s) 365, 526
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 2 cut(s) 95, 167
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 362
AspS9I GGNCC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 1 cut(s) 584
AsuII TTCGAA 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 269
BbvI GCAGC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 470
BmrFI CCNGG 1 cut(s) 468
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 2 cut(s) 29, 237
BmuI ACTGGG 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 93
Bpu14I TTCGAA 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 607
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 4 cut(s) 110, 148, 214, 451
Bse8I GATNNNNATC 1 cut(s) 99
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 4 cut(s) 110, 148, 214, 451
BseXI GCAGC 1 cut(s) 374
BshFI GGCC 1 cut(s) 471
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 53
BsnI GGCC 1 cut(s) 471
Bsp119I TTCGAA 1 cut(s) 389
Bsp143I GATC 3 cut(s) 100, 159, 334
BspACI CCGC 3 cut(s) 105, 570, 597
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 1 cut(s) 57
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 2 cut(s) 95, 167
BspT104I TTCGAA 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 107
BsrI ACTGG 4 cut(s) 110, 148, 214, 451
BssMI GATC 3 cut(s) 100, 159, 334
BssNI GRCGYC 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 435, 544
BstACI GRCGYC 1 cut(s) 607
BstAPI GCANNNNNTGC 1 cut(s) 89
BstBI TTCGAA 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 44, 617
BstKTI GATC 3 cut(s) 103, 162, 337
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 3 cut(s) 100, 159, 334
BstMWI GCNNNNNNNGC 3 cut(s) 89, 459, 468
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 374
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 117, 207, 330
Cfr13I GGNCC 1 cut(s) 470
CseI GACGC 1 cut(s) 596
Csp6I GTAC 1 cut(s) 626
CviAII CATG 2 cut(s) 89, 640
CviJI RGCY 6 cut(s) 70, 193, 203, 365, 471, 526
CviKI_1 RGCY 6 cut(s) 70, 193, 203, 365, 471, 526
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 2 cut(s) 44, 617
DpnI GATC 3 cut(s) 102, 161, 336
DpnII GATC 3 cut(s) 100, 159, 334
Eco57I CTGAAG 2 cut(s) 43, 326
FaeI CATG 2 cut(s) 92, 643
FaiI YATR 8 cut(s) 90, 132, 482, 510, 547, 566, 568, 641
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 2 cut(s) 88, 639
FauI CCCGC 2 cut(s) 112, 590
Fnu4HI GCNGC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 200
GluI GCNGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 1 cut(s) 471
HapII CCGG 1 cut(s) 468
HgaI GACGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 607
Hin1II CATG 2 cut(s) 92, 643
HinfI GANTC 4 cut(s) 95, 301, 419, 651
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 584
Hpy188I TCNGA 5 cut(s) 62, 100, 306, 381, 656
Hpy188III TCNNGA 5 cut(s) 251, 442, 496, 590, 646
HpyAV CCTTC 4 cut(s) 67, 385, 452, 587
HpyCH4III ACNGT 2 cut(s) 435, 544
HpyCH4V TGCA 4 cut(s) 12, 83, 362, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 459, 468
HpyF3I CTNAG 2 cut(s) 44, 617
Hsp92I GRCGYC 1 cut(s) 607
Hsp92II CATG 2 cut(s) 92, 643
Kzo9I GATC 3 cut(s) 100, 159, 334
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 1 cut(s) 374
LweI GCATC 2 cut(s) 29, 237
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 367, 439
MalI GATC 3 cut(s) 102, 161, 336
MbiI CCGCTC 1 cut(s) 107
MboI GATC 3 cut(s) 100, 159, 334
MboII GAAGA 2 cut(s) 269, 319
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 159
MluCI AATT 2 cut(s) 487, 550
MnlI CCTC 4 cut(s) 53, 56, 176, 256
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 1 cut(s) 468
MunI CAATTG 1 cut(s) 487
MwoI GCNNNNNNNGC 3 cut(s) 89, 459, 468
NciI CCSGG 1 cut(s) 468
NdeII GATC 3 cut(s) 100, 159, 334
NlaIII CATG 2 cut(s) 92, 643
NmuCI GTSAC 1 cut(s) 439
NspV TTCGAA 1 cut(s) 389
PfeI GAWTC 3 cut(s) 95, 301, 419
PkrI GCNGC 1 cut(s) 364
PspPI GGNCC 1 cut(s) 470
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 3 cut(s) 100, 159, 334
Sau96I GGNCC 1 cut(s) 470
ScrFI CCNGG 1 cut(s) 468
SfaNI GCATC 2 cut(s) 29, 237
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 389
Sse9I AATT 2 cut(s) 487, 550
SsiI CCGC 3 cut(s) 105, 570, 597
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 435, 544
TaqI TCGA 1 cut(s) 389
TasI AATT 2 cut(s) 487, 550
TfiI GAWTC 3 cut(s) 95, 301, 419
TscAI CASTG 3 cut(s) 117, 214, 337
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 1 cut(s) 362
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 1 cut(s) 432
TspRI CASTG 3 cut(s) 117, 214, 337
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.