FvH4_5g18510

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017494
Physical Location & Seq
Reverse (-)
10651045 .. 10651725
681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18510.t1

Sequence Viewer

Length: 681 bp
ATGTTTCTCTCTAGATTTCCAGCTGTGAGGCTCTTGACATTGGCATCACAAGTCCTTATCTGCTATCTCAGTCTCCTTGCTTTCAGAGGAAAGCCATTGTTGGTTGCAGTTCATGCTGCTGATCCTGCTCCAGTGCGTGACATCAAAGGTGACAATCTCCAAACTGGGGTTGAGTATTGGATCTTGCCTGTCATCCGTGGGAAAGGTGGGGGGCTAACACTAGCCAGCAATAGAAATAAAACCATCGGCTGCCCACTTGATGTTGTTCAAGAGCAAAGCGAGGTATCTAATGGTCTTCCACTCACATTTTCACCTGTGAATCTGACGAAAGGCGTCGTTCGCCTATCCACTGATCTTAACATCAAGTTCTCTACATCTGCAGCTGTAACTCTCTGTCCGCAGACTTCAAAGGTTTGGAAGTTAGACGAGGTTGATGAATCAACAGGGCAACGGTCTGTCACGACTGGTGGCGTAGAAGGCAATCCGAGTCCTAAAACTCTCATGAACTGGTTTAAGATCGAGAAATATGATGATGACTACAAGCTCGTCTTTTGCCCCAAAGTCTGTCGCTTCTGCAGGGTTATATGTGGTGATGTTGGCGTTCATCTTGAAAGTGGGGTTAGGCGTCTGGCTCTAAGTGATGTAATTGTACCACTCAAAGTCATGTTCAAGAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kunitz_legume

Protein Analysis

227

Amino Acids

24.78

Weight (kDa)

9.21

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 45 - 225 3.8e-57 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17860
fragaria_vesca FvH4_5g18500 FvH4_5g18510
prunus_persica Prupe.5G074400_v2.0.a1 Prupe.5G074500_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177011
rosa_laevigata RLG00000005564
rosa_samantha Rh7AG002400 Rh7BG002700 Rh7CG002000 Rh7DG002600
rosa_wichuraiana Rw7G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 398
AclWI GGATC 2 cut(s) 116, 188
AcyI GRCGYC 2 cut(s) 333, 625
AfaI GTAC 1 cut(s) 651
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 4 cut(s) 269, 408, 611, 670
AluBI AGCT 3 cut(s) 23, 383, 544
AluI AGCT 3 cut(s) 23, 383, 544
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 2 cut(s) 116, 188
ApeKI GCWGC 3 cut(s) 116, 249, 380
AsuHPI GGTGA 3 cut(s) 161, 303, 602
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 103, 236, 392
BccI CCATC 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 2 cut(s) 12, 221
BfmI CTRYAG 2 cut(s) 378, 574
BisI GCNGC 3 cut(s) 117, 250, 381
BlsI GCNGC 3 cut(s) 118, 251, 382
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 53
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 114
BsaHI GRCGYC 2 cut(s) 333, 625
BsaJI CCNNGG 1 cut(s) 196
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 4 cut(s) 131, 169, 469, 512
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 4 cut(s) 131, 169, 469, 512
BseXI GCAGC 3 cut(s) 103, 236, 392
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 121, 180, 352, 516
BspACI CCGC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 81
BspHI TCATGA 1 cut(s) 501
BspMAI CTGCAG 2 cut(s) 382, 578
BspPI GGATC 2 cut(s) 116, 188
BsrI ACTGG 4 cut(s) 131, 169, 469, 512
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 4 cut(s) 121, 180, 352, 516
BssNI GRCGYC 2 cut(s) 333, 625
Bst4CI ACNGT 1 cut(s) 453
BstACI GRCGYC 2 cut(s) 333, 625
BstAPI GCANNNNNTGC 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 68, 635
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 1 cut(s) 192
BstKTI GATC 4 cut(s) 124, 183, 355, 519
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 4 cut(s) 121, 180, 352, 516
BstMWI GCNNNNNNNGC 4 cut(s) 113, 125, 339, 477
BstSFI CTRYAG 2 cut(s) 378, 574
BstV1I GCAGC 3 cut(s) 103, 236, 392
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 138, 348
Cac8I GCNNGC 1 cut(s) 226
CciI TCATGA 1 cut(s) 501
CseI GACGC 2 cut(s) 322, 614
Csp6I GTAC 1 cut(s) 650
CviAII CATG 3 cut(s) 113, 502, 664
CviJI RGCY 9 cut(s) 23, 31, 94, 214, 224, 249, 383, 544, 632
CviKI_1 RGCY 9 cut(s) 23, 31, 94, 214, 224, 249, 383, 544, 632
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 2 cut(s) 68, 635
DpnI GATC 4 cut(s) 123, 182, 354, 518
DpnII GATC 4 cut(s) 121, 180, 352, 516
FaeI CATG 3 cut(s) 116, 505, 667
FaiI YATR 6 cut(s) 114, 503, 528, 584, 586, 665
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 3 cut(s) 112, 501, 663
Fnu4HI GCNGC 3 cut(s) 117, 250, 381
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 3 cut(s) 117, 250, 381
FspBI CTAG 2 cut(s) 12, 221
GluI GCNGC 3 cut(s) 117, 250, 381
GsuI CTGGAG 1 cut(s) 114
HgaI GACGC 2 cut(s) 322, 614
Hin1I GRCGYC 2 cut(s) 333, 625
Hin1II CATG 3 cut(s) 116, 505, 667
HinfI GANTC 4 cut(s) 319, 437, 487, 675
HphI GGTGA 3 cut(s) 161, 303, 602
Hpy188I TCNGA 4 cut(s) 86, 324, 486, 680
Hpy188III TCNNGA 8 cut(s) 12, 34, 269, 460, 502, 520, 608, 670
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 470
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4V TGCA 3 cut(s) 107, 380, 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 125, 339, 477
HpyF3I CTNAG 2 cut(s) 68, 635
Hsp92I GRCGYC 2 cut(s) 333, 625
Hsp92II CATG 3 cut(s) 116, 505, 667
Kzo9I GATC 4 cut(s) 121, 180, 352, 516
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 3 cut(s) 103, 236, 392
LweI GCATC 1 cut(s) 53
MaeI CTAG 2 cut(s) 12, 221
MaeIII GTNAC 4 cut(s) 137, 149, 385, 457
MalI GATC 4 cut(s) 123, 182, 354, 518
MboI GATC 4 cut(s) 121, 180, 352, 516
MboII GAAGA 1 cut(s) 287
MflI RGATCY 1 cut(s) 180
MluCI AATT 1 cut(s) 645
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 4 cut(s) 21, 80, 274, 421
MseI TTAA 2 cut(s) 357, 513
MspA1I CMGCKG 2 cut(s) 23, 383
MwoI GCNNNNNNNGC 4 cut(s) 113, 125, 339, 477
NdeII GATC 4 cut(s) 121, 180, 352, 516
NlaIII CATG 3 cut(s) 116, 505, 667
NmuCI GTSAC 3 cut(s) 137, 149, 457
PagI TCATGA 1 cut(s) 501
PfeI GAWTC 2 cut(s) 319, 437
PkrI GCNGC 3 cut(s) 118, 251, 382
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PstI CTGCAG 2 cut(s) 382, 578
PsuI RGATCY 1 cut(s) 180
PvuII CAGCTG 2 cut(s) 23, 383
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
SaqAI TTAA 2 cut(s) 357, 513
SatI GCNGC 3 cut(s) 117, 250, 381
Sau3AI GATC 4 cut(s) 121, 180, 352, 516
SchI GAGTC 1 cut(s) 496
SetI ASST 9 cut(s) 25, 151, 208, 285, 316, 385, 414, 432, 546
SfaNI GCATC 1 cut(s) 53
SfcI CTRYAG 2 cut(s) 378, 574
Sse9I AATT 1 cut(s) 645
SsiI CCGC 1 cut(s) 398
SspMI CTAG 2 cut(s) 12, 221
TaaI ACNGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 519
TasI AATT 1 cut(s) 645
TfiI GAWTC 2 cut(s) 319, 437
Tru1I TTAA 2 cut(s) 357, 513
Tru9I TTAA 2 cut(s) 357, 513
TscAI CASTG 2 cut(s) 138, 355
TseFI GTSAC 3 cut(s) 137, 149, 457
TseI GCWGC 3 cut(s) 116, 249, 380
Tsp45I GTSAC 3 cut(s) 137, 149, 457
TspDTI ATGAA 4 cut(s) 101, 450, 518, 593
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 2 cut(s) 138, 355
XbaI TCTAGA 1 cut(s) 11
XspI CTAG 2 cut(s) 12, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.