AT1G18520

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013325
Physical Location & Seq
Forward (+)
6374906 .. 6376333
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18520.1

Sequence Viewer

Length: 816 bp
ATGTTTCGAGTTAGCAATTTCATGGTTGGTCTAGCAAACACATTGGTGATGTTAGTGGGCGCTTCGGCCATTGGTTATTCGATTTACATGTTCGTTCACCAAGGCGTCACTGATTGTGAATCTGCCATTCGGATACCACTTCTCACGACCGGACTCATCCTCTTCTTGGTGTCTTTGCTCGGAGTGATTGGATCTTGTTTCAAGGAGAATTTGGCAATGGTTTCCTACTTGATCATATTGTTTGGGGGCATTGTTGCATTGATGATTTTCTCCATATTTCTCTTCTTTGTGACCAACAAAGGAGCCGGTCGTGTGGTGTCCGGTCGAGGGTATAAAGAGTACCGGACGGTGGATTTCTCGACGTGGCTTAATGGGTTCGTTGGTGGGAAGAGATGGGTTGGGATAAGGTCTTGTTTGGCTGAGGCTAACGTTTGTGATGATTTGAGTGATGGTCGTGTTAGTCAGATCGCTGATGCGTTTTATCACAAGAACTTGTCTCCCATCCAGTCAGGTTGTTGTAAGCCACCATCGGATTGCAACTTCGAGTTCAGAAACGCGACGTTCTGGATACCGCCGAGCAAAAACGAAACGGCAGTTGCGGAAAACGGGGACTGTGGTACGTGGAGCAACGTGCAAACAGAGTTATGTTTCAACTGCAACGCATGCAAAGCGGGTGTGTTAGCGAACATAAGAGAGAAGTGGAGGAATCTTCTTGTTTTCAACATTTGTCTCCTCATTCTCCTCATAACCGTCTATTCCTGCGGTTGCTGTGCTCGTCGTAACAATCGGACGGCTAGGAAAAGTGATTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

271

Amino Acids

29.82

Weight (kDa)

8.72

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 258 4.7e-30 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18520
fragaria_vesca FvH4_5g12970
malus_domestica MD06G1208400.v1.1
prunus_persica Prupe.5G212900_v2.0.a1
pyrus_communis pycom06g18620
rosa_chinensis RchiOBHm_Chr7g0184191
rosa_laevigata RLG00000004995
rosa_multiflora Rmu_co8233853.1_g000001 Rmu_co8323147.1_g000001 Rmu_sc0012221.1_g000007 Rmu_sc0013812.1_g000001
rosa_roxburghii Rroxscaffold_3G00269810
rosa_rugosa Rorug06G0463800
rosa_samantha Rh7AG082100 Rh7BG065100 Rh7CG065500 Rh7DG065200
rosa_wichuraiana Rw7G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 557
AciI CCGC 4 cut(s) 572, 599, 671, 762
AclI AACGTT 1 cut(s) 429
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 208
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 2 cut(s) 341, 619
AfiI CCNNNNNNNGG 3 cut(s) 166, 327, 349
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 3 cut(s) 202, 652, 721
AjiI CACGTC 1 cut(s) 363
AleI CACNNNNGTG 1 cut(s) 44
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 2 cut(s) 501, 734
AlwI GGATC 1 cut(s) 199
AoxI GGCC 1 cut(s) 66
ApoI RAATTY 1 cut(s) 208
AspLEI GCGC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 58, 89
Bbv12I GWGCWC 1 cut(s) 775
BbvCI CCTCAGC 1 cut(s) 420
BccI CCATC 4 cut(s) 387, 443, 509, 535
BceAI ACGGC 2 cut(s) 606, 807
BciVI GTATCC 2 cut(s) 126, 561
BclI TGATCA 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 501, 734
BfaI CTAG 2 cut(s) 32, 795
BfoI RGCGCY 1 cut(s) 63
BfuI GTATCC 2 cut(s) 126, 561
BmgBI CACGTC 1 cut(s) 363
BmiI GGNNCC 1 cut(s) 304
BmsI GCATC 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 3 cut(s) 149, 320, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 327, 349
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 156, 501
BseLI CCNNNNNNNGG 3 cut(s) 166, 327, 349
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 2 cut(s) 722, 731
Bsh1236I CGCG 1 cut(s) 557
Bsh1285I CGRYCG 3 cut(s) 150, 310, 325
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 3 cut(s) 150, 310, 325
BsiHKAI GWGCWC 1 cut(s) 775
BsiSI CCGG 4 cut(s) 150, 306, 321, 343
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 3 cut(s) 166, 327, 349
BsmAI GTCTC 2 cut(s) 501, 734
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 3 cut(s) 191, 231, 465
BspACI CCGC 4 cut(s) 572, 599, 671, 762
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 1 cut(s) 304
BspPI GGATC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 1 cut(s) 505
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 3 cut(s) 191, 231, 465
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 100
Bst4CI ACNGT 3 cut(s) 349, 614, 751
Bst6I CTCTTC 3 cut(s) 167, 287, 383
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 663
BstBAI YACGTR 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 156, 501
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 3 cut(s) 194, 234, 468
BstMAI GTCTC 2 cut(s) 501, 734
BstMBI GATC 3 cut(s) 191, 231, 465
BstMCI CGRYCG 3 cut(s) 150, 310, 325
BstMWI GCNNNNNNNGC 2 cut(s) 663, 668
BstNSI RCATGY 2 cut(s) 91, 666
BstUI CGCG 1 cut(s) 557
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
BsuI GTATCC 2 cut(s) 126, 561
BsuRI GGCC 1 cut(s) 68
BtrI CACGTC 1 cut(s) 363
BtsCI GGATG 2 cut(s) 156, 501
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 664
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 305
CseI GACGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 340, 618
CviAII CATG 3 cut(s) 22, 88, 663
CviJI RGCY 7 cut(s) 68, 305, 367, 419, 425, 523, 794
CviKI_1 RGCY 7 cut(s) 68, 305, 367, 419, 425, 523, 794
CviQI GTAC 2 cut(s) 340, 618
DdeI CTNAG 1 cut(s) 420
DpnI GATC 3 cut(s) 193, 233, 467
DpnII GATC 3 cut(s) 191, 231, 465
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 3 cut(s) 167, 287, 383
EarI CTCTTC 3 cut(s) 167, 287, 383
Eco130I CCWWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 100
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 3 cut(s) 25, 91, 666
FaiI YATR 9 cut(s) 23, 89, 236, 275, 333, 646, 664, 689, 746
FaqI GGGAC 1 cut(s) 623
FatI CATG 3 cut(s) 21, 87, 662
FauI CCCGC 1 cut(s) 664
FbaI TGATCA 1 cut(s) 231
FokI GGATG 2 cut(s) 143, 488
FspBI CTAG 2 cut(s) 32, 795
GlaI GCGC 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 1 cut(s) 68
HapII CCGG 4 cut(s) 150, 306, 321, 343
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 3 cut(s) 25, 91, 666
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HinfI GANTC 4 cut(s) 119, 153, 706, 806
HpaII CCGG 4 cut(s) 150, 306, 321, 343
HphI GGTGA 2 cut(s) 58, 89
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 7 cut(s) 132, 182, 465, 532, 551, 789, 815
Hpy188III TCNNGA 3 cut(s) 145, 358, 565
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 3 cut(s) 364, 562, 780
HpyCH4III ACNGT 3 cut(s) 349, 614, 751
HpyCH4IV ACGT 5 cut(s) 362, 429, 560, 620, 630
HpyCH4V TGCA 5 cut(s) 257, 537, 634, 657, 666
HpyF10VI GCNNNNNNNGC 2 cut(s) 663, 668
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 5 cut(s) 362, 429, 560, 620, 630
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 3 cut(s) 25, 91, 666
HspAI GCGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 231
Kzo9I GATC 3 cut(s) 191, 231, 465
LmnI GCTCC 2 cut(s) 302, 624
LpnPI CCDG 8 cut(s) 163, 319, 334, 356, 495, 518, 550, 772
LweI GCATC 1 cut(s) 463
MaeI CTAG 2 cut(s) 32, 795
MaeII ACGT 5 cut(s) 362, 429, 560, 620, 630
MaeIII GTNAC 3 cut(s) 106, 289, 779
MalI GATC 3 cut(s) 193, 233, 467
MboI GATC 3 cut(s) 191, 231, 465
MboII GAAGA 4 cut(s) 154, 274, 400, 701
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 2 cut(s) 16, 208
MlyI GAGTC 1 cut(s) 147
MnlI CCTC 6 cut(s) 170, 320, 415, 696, 743, 752
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 4 cut(s) 150, 306, 321, 343
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 2 cut(s) 663, 668
NdeII GATC 3 cut(s) 191, 231, 465
NlaIII CATG 3 cut(s) 25, 91, 666
NlaIV GGNNCC 1 cut(s) 304
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 106, 289
NspI RCATGY 2 cut(s) 91, 666
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 1 cut(s) 666
PciI ACATGT 1 cut(s) 87
PfeI GAWTC 3 cut(s) 119, 706, 806
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
Ppu21I YACGTR 1 cut(s) 621
PscI ACATGT 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 191
RsaI GTAC 2 cut(s) 341, 619
RsaNI GTAC 2 cut(s) 340, 618
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 3 cut(s) 191, 231, 465
SchI GAGTC 1 cut(s) 147
SduI GDGCHC 1 cut(s) 775
SetI ASST 7 cut(s) 365, 410, 432, 514, 563, 623, 633
SfaNI GCATC 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 44
SphI GCATGC 1 cut(s) 666
Sse9I AATT 2 cut(s) 16, 208
SsiI CCGC 4 cut(s) 572, 599, 671, 762
SspMI CTAG 2 cut(s) 32, 795
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 349, 614, 751
TaiI ACGT 5 cut(s) 365, 432, 563, 623, 633
TaqI TCGA 5 cut(s) 7, 80, 325, 359, 543
TasI AATT 2 cut(s) 16, 208
TfiI GAWTC 3 cut(s) 119, 706, 806
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 106, 289
Tsp45I GTSAC 2 cut(s) 106, 289
TspDTI ATGAA 1 cut(s) 10
TspRI CASTG 1 cut(s) 115
XapI RAATTY 1 cut(s) 208
XceI RCATGY 2 cut(s) 91, 666
XspI CTAG 2 cut(s) 32, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.