FvH4_5g12970

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013325
Physical Location & Seq
Reverse (-)
7332391 .. 7334353
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12970.t1

Sequence Viewer

Length: 819 bp
ATGTTACGTATAAGCAACACCGTGATCGGGATCCTGAACGCGATGTTCCTCCTCCTCGGACTCGCCTCCATCGGCGCGTCGATCTACTTCCACCTCCACGGCGGCACCATGTGCCAGAAGGTCCTCCAGAACCCTCTCCTCTTCGCCGGACTCGTCTTCGTCGTCGTGTCCCTCCTCGGCCTCGCCGGCTCGTGCTGCAAACTCAACTCTCTCCTCTATACCTACCTCGTCTTGATCTTTCTCCTCATCCTCGGATTCATCGCCCTCACCGTGTTCGCTCTGTTCGTGACGAACAAGGGGATCGGACAGGCCGTGTCGGGACGAGGGTACAAGGAGTACAAGGTCGGAGACTTCTCGCATTGGCTTCAGCATTACGTTGTTAATGGGAAAAACTGGGAGCCGATCAAGAGTTGTCTAGTGGATGCTCAGGTTTGTAAGAGTCTCGGTGGCGATGTTCATCAGACTCAGGCCGATTTTTATAGGAAGAACTTGTCTCCGATTCAGTCTGGTTGCTGCAAGCCACCATCCTACTGCGGATATGAATTCAAGAATGCAACATACTGGGCGATTCCGAAGTCTGGACCTAAAGAGCCGGACACTGACTGCCTAACTTGGAGTAACAAGCAGAGCAGGTTGTGCTACGACTGCAAGTCATGCAAAGGAGGAATTCTGGCCAACATTCGCAAGCAGTGGCGGTCTCTCGCCATACTCAACTGCTGTATTCTTGTGCTCCTTACCCTTGTCTACTGCGTTGGCTGCTGTGCTACCAAGAACAATCGCTTCAGCACTTACAACAACAGCAAGTTCCGCGGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

273

Amino Acids

30.08

Weight (kDa)

9.21

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 255 4e-32 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18520
fragaria_vesca FvH4_5g12970
malus_domestica MD06G1208400.v1.1
prunus_persica Prupe.5G212900_v2.0.a1
pyrus_communis pycom06g18620
rosa_chinensis RchiOBHm_Chr7g0184191
rosa_laevigata RLG00000004995
rosa_multiflora Rmu_co8233853.1_g000001 Rmu_co8323147.1_g000001 Rmu_sc0012221.1_g000007 Rmu_sc0013812.1_g000001
rosa_roxburghii Rroxscaffold_3G00269810
rosa_rugosa Rorug06G0463800
rosa_samantha Rh7AG082100 Rh7BG065100 Rh7CG065500 Rh7DG065200
rosa_wichuraiana Rw7G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 744
AccII CGCG 3 cut(s) 41, 77, 810
AciI CCGC 5 cut(s) 102, 534, 694, 808, 810
AclWI GGATC 3 cut(s) 25, 38, 308
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 2 cut(s) 542, 666
AcuI CTGAAG 2 cut(s) 350, 766
AdeI CACNNNGTG 1 cut(s) 111
AfaI GTAC 2 cut(s) 329, 338
AfiI CCNNNNNNNGG 2 cut(s) 27, 578
AgsI TTSAA 1 cut(s) 547
AhdI GACNNNNNGTC 1 cut(s) 649
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 4 cut(s) 342, 446, 498, 702
AlwI GGATC 3 cut(s) 25, 38, 308
AoxI GGCC 5 cut(s) 178, 309, 468, 672, 812
ApeKI GCWGC 3 cut(s) 195, 513, 756
ApoI RAATTY 2 cut(s) 542, 666
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 3 cut(s) 121, 581, 812
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 121, 581
BalI TGGCCA 1 cut(s) 674
BamHI GGATCC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 104
BauI CACGAG 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 182, 500, 743
BccI CCATC 2 cut(s) 77, 532
BceAI ACGGC 2 cut(s) 115, 296
BcoDI GTCTC 4 cut(s) 342, 446, 498, 702
BfaI CTAG 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 621
BglI GCCNNNNNGGC 1 cut(s) 186
BisI GCNGC 4 cut(s) 103, 196, 514, 757
BlsI GCNGC 4 cut(s) 104, 197, 515, 758
Bme18I GGWCC 2 cut(s) 121, 581
BmeRI GACNNNNNGTC 1 cut(s) 649
BmgT120I GGNCC 3 cut(s) 121, 581, 812
BmiI GGNNCC 3 cut(s) 32, 106, 399
BmrI ACTGGG 2 cut(s) 403, 571
BmsI GCATC 1 cut(s) 412
BmuI ACTGGG 2 cut(s) 403, 571
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 426
BsaAI YACGTR 1 cut(s) 8
BsaBI GATNNNNATC 2 cut(s) 29, 456
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 5 cut(s) 55, 97, 175, 250, 808
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 578
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 398, 566
Bse8I GATNNNNATC 2 cut(s) 29, 456
BseDI CCNNGG 5 cut(s) 55, 97, 175, 250, 808
BseGI GGATG 3 cut(s) 246, 427, 524
BseJI GATNNNNATC 2 cut(s) 29, 456
BseLI CCNNNNNNNGG 2 cut(s) 27, 578
BseMII CTCAG 2 cut(s) 440, 479
BseNI ACTGG 2 cut(s) 398, 566
BseRI GAGGAG 6 cut(s) 41, 44, 128, 164, 203, 233
BseXI GCAGC 3 cut(s) 182, 500, 743
Bsh1236I CGCG 3 cut(s) 41, 77, 810
BshFI GGCC 5 cut(s) 180, 311, 470, 674, 814
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 3 cut(s) 147, 186, 593
BslFI GGGAC 2 cut(s) 154, 333
BslI CCNNNNNNNGG 2 cut(s) 27, 578
BsmAI GTCTC 4 cut(s) 342, 446, 498, 702
BsmFI GGGAC 2 cut(s) 154, 333
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 5 cut(s) 180, 311, 470, 674, 814
Bso31I GGTCTC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 6 cut(s) 24, 30, 81, 234, 300, 402
BspACI CCGC 5 cut(s) 102, 534, 694, 808, 810
BspANI GGCC 5 cut(s) 180, 311, 470, 674, 814
BspCNI CTCAG 2 cut(s) 439, 478
BspFNI CGCG 3 cut(s) 41, 77, 810
BspLI GGNNCC 3 cut(s) 32, 106, 399
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 3 cut(s) 25, 38, 308
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 702
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 398, 566
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 5 cut(s) 55, 97, 175, 250, 808
BssMI GATC 6 cut(s) 24, 30, 81, 234, 300, 402
BssSI CACGAG 1 cut(s) 190
Bst2BI CACGAG 1 cut(s) 190
Bst4CI ACNGT 2 cut(s) 22, 271
Bst6I CTCTTC 1 cut(s) 146
BstAPI GCANNNNNTGC 3 cut(s) 111, 636, 654
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 4 cut(s) 187, 518, 686, 812
BstDEI CTNAG 2 cut(s) 426, 465
BstDSI CCRYGG 2 cut(s) 97, 808
BstF5I GGATG 3 cut(s) 246, 427, 524
BstFNI CGCG 3 cut(s) 41, 77, 810
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 6 cut(s) 27, 33, 84, 237, 303, 405
BstMAI GTCTC 4 cut(s) 342, 446, 498, 702
BstMBI GATC 6 cut(s) 24, 30, 81, 234, 300, 402
BstMWI GCNNNNNNNGC 8 cut(s) 111, 186, 195, 636, 645, 654, 756, 807
BstSNI TACGTA 1 cut(s) 8
BstUI CGCG 3 cut(s) 41, 77, 810
BstV1I GCAGC 3 cut(s) 182, 500, 743
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 5 cut(s) 180, 311, 470, 674, 814
BtgI CCRYGG 2 cut(s) 97, 808
BtgZI GCGATG 3 cut(s) 56, 244, 465
BtsCI GGATG 3 cut(s) 246, 427, 524
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 2 cut(s) 597, 695
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 4 cut(s) 187, 518, 686, 812
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 121, 581, 812
Cfr42I CCGCGG 1 cut(s) 811
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 328, 337
CviAII CATG 2 cut(s) 109, 654
CviQI GTAC 2 cut(s) 328, 337
DdeI CTNAG 2 cut(s) 426, 465
DpnI GATC 6 cut(s) 26, 32, 83, 236, 302, 404
DpnII GATC 6 cut(s) 24, 30, 81, 234, 300, 402
DraIII CACNNNGTG 1 cut(s) 111
DriI GACNNNNNGTC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 146
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 146
Eco105I TACGTA 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 121, 581
Eco57I CTGAAG 2 cut(s) 350, 766
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 2 cut(s) 542, 666
FaeI CATG 2 cut(s) 112, 657
FaiI YATR 8 cut(s) 11, 110, 219, 480, 540, 559, 655, 707
FaqI GGGAC 2 cut(s) 154, 333
FatI CATG 2 cut(s) 108, 653
FauI CCCGC 1 cut(s) 803
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 4 cut(s) 103, 196, 514, 757
FokI GGATG 3 cut(s) 233, 434, 511
Fsp4HI GCNGC 4 cut(s) 103, 196, 514, 757
FspBI CTAG 1 cut(s) 416
GlaI GCGC 1 cut(s) 76
GluI GCNGC 4 cut(s) 103, 196, 514, 757
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 5 cut(s) 180, 311, 470, 674, 814
HapII CCGG 3 cut(s) 147, 186, 593
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 77
Hin1II CATG 2 cut(s) 112, 657
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HinfI GANTC 7 cut(s) 60, 150, 255, 439, 463, 499, 568
HpaII CCGG 3 cut(s) 147, 186, 593
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 7 cut(s) 59, 254, 305, 347, 462, 498, 573
Hpy188III TCNNGA 9 cut(s) 28, 34, 127, 232, 286, 318, 406, 547, 579
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 3 cut(s) 82, 164, 167
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 2 cut(s) 22, 271
HpyCH4IV ACGT 2 cut(s) 7, 375
HpyCH4V TGCA 5 cut(s) 198, 516, 554, 648, 657
HpyF10VI GCNNNNNNNGC 8 cut(s) 111, 186, 195, 636, 645, 654, 756, 807
HpyF3I CTNAG 2 cut(s) 426, 465
HpySE526I ACGT 2 cut(s) 7, 375
Hsp92II CATG 2 cut(s) 112, 657
HspAI GCGC 1 cut(s) 75
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
KspI CCGCGG 1 cut(s) 811
Kzo9I GATC 6 cut(s) 24, 30, 81, 234, 300, 402
LmnI GCTCC 2 cut(s) 397, 735
Lsp1109I GCAGC 3 cut(s) 182, 500, 743
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 416
MaeII ACGT 2 cut(s) 7, 375
MaeIII GTNAC 3 cut(s) 3, 286, 617
MalI GATC 6 cut(s) 26, 32, 83, 236, 302, 404
MboI GATC 6 cut(s) 24, 30, 81, 234, 300, 402
MboII GAAGA 3 cut(s) 133, 148, 496
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 732
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 2 cut(s) 542, 666
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 4 cut(s) 54, 144, 448, 457
MmeI TCCRAC 1 cut(s) 325
Mox20I TGGCCA 1 cut(s) 674
MroNI GCCGGC 1 cut(s) 185
MscI TGGCCA 1 cut(s) 674
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 810
MspI CCGG 3 cut(s) 147, 186, 593
Mva1269I GAATGC 1 cut(s) 556
MvnI CGCG 3 cut(s) 41, 77, 810
MwoI GCNNNNNNNGC 8 cut(s) 111, 186, 195, 636, 645, 654, 756, 807
NaeI GCCGGC 1 cut(s) 187
NdeII GATC 6 cut(s) 24, 30, 81, 234, 300, 402
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 2 cut(s) 112, 657
NlaIV GGNNCC 3 cut(s) 32, 106, 399
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 5 cut(s) 150, 159, 267, 282, 309
PctI GAATGC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 3 cut(s) 255, 499, 568
PkrI GCNGC 4 cut(s) 104, 197, 515, 758
PleI GAGTC 4 cut(s) 54, 144, 447, 457
PpsI GAGTC 4 cut(s) 54, 144, 447, 457
Ppu21I YACGTR 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 32, 106, 399
PspPI GGNCC 3 cut(s) 121, 581, 812
PspPPI RGGWCCY 1 cut(s) 121
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 2 cut(s) 329, 338
RsaNI GTAC 2 cut(s) 328, 337
SacII CCGCGG 1 cut(s) 811
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 4 cut(s) 103, 196, 514, 757
Sau3AI GATC 6 cut(s) 24, 30, 81, 234, 300, 402
Sau96I GGNCC 3 cut(s) 121, 581, 812
SchI GAGTC 4 cut(s) 54, 144, 448, 457
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 1 cut(s) 412
Sfr303I CCGCGG 1 cut(s) 811
SgrBI CCGCGG 1 cut(s) 811
SinI GGWCC 2 cut(s) 121, 581
SnaBI TACGTA 1 cut(s) 8
Sse9I AATT 2 cut(s) 542, 666
SsiI CCGC 5 cut(s) 102, 534, 694, 808, 810
SspMI CTAG 1 cut(s) 416
TaaI ACNGT 2 cut(s) 22, 271
TaiI ACGT 2 cut(s) 10, 378
TaqI TCGA 1 cut(s) 80
TasI AATT 2 cut(s) 542, 666
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 3 cut(s) 255, 499, 568
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 604, 695
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 195, 513, 756
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 3 cut(s) 247, 446, 555
TspRI CASTG 2 cut(s) 604, 695
VpaK11BI GGWCC 2 cut(s) 121, 581
XapI RAATTY 2 cut(s) 542, 666
XmiI GTMKAC 1 cut(s) 744
XspI CTAG 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.