MD06G1208400.v1.1

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013325
Physical Location & Seq
Forward (+)
34227715 .. 34229414
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1208400.v1.1.491

Sequence Viewer

Length: 816 bp
ATGTTTCGCGTAAGCAACGCGGTGGTGGGGATCTTAAACTTTCTCTTCCTCCTCCTCGGCCTCGCCTCCATCGGGGCCGGGATCTACTTCCAAATCCACGGCGGCACCCACTGCCAAAAAGTTCTCCAAACTCCGCTCATCATCGGCGGCGTCTTCTTCTGCGTGGTGTCCCTTCTAGGGCTCACCGGCTCCTGCTGCCGACTCAACTCTCTCCTCTACATCTACTTAATCGTTGTCTTCGTGGTCATCATCGGGCTCATCGTGTTCACCGTGTTCGCCATTCTCGTGACGAACAAGGGGATAGGGCAGGTGGTTTCCGGGAGAGGGTACAAGGAGTACAAGAGCGGAGACTTCTCGCACTGGATGCAGCATTACGTGGTGAATGATAAGAACTGGGGTCCCATCAAGAGTTGCTTGATCGATGCGCAGATTTGCAAGAGTCTTGGCGGCGACGTTCATGAATCCCAGGCCGAATTCTACAAGAAAAACTTGTCTCCTATACAGTCTGGTTGCTGCAAGCCGCCGTCCTACTGCGGATTCGAGTACAAGAACGCGACGTTCTGGGAAGTTCCGAAAAAAGGGCCGGCGGTCGGAGACAGCGACTGCAAAACGTGGAGCAACGAGCAGAAGAAGCTGTGCTACGAGTGCGAGTCGTGCAAGGGAGGAGTGCTGGCAAACATCAGGAAGCAGTGGAGGAACTTGGCCATATTTAACGGGTGTGTTATTGTACTGGTGATTCTCATCTACTGCGTCGGCTGCTGTGCTACCAAGAACAATCGCTTCAGTACTTACAACAAATTTAGAGGCGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

272

Amino Acids

29.9

Weight (kDa)

9.04

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 255 2.1e-33 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18520
fragaria_vesca FvH4_5g12970
malus_domestica MD06G1208400.v1.1
prunus_persica Prupe.5G212900_v2.0.a1
pyrus_communis pycom06g18620
rosa_chinensis RchiOBHm_Chr7g0184191
rosa_laevigata RLG00000004995
rosa_multiflora Rmu_co8233853.1_g000001 Rmu_co8323147.1_g000001 Rmu_sc0012221.1_g000007 Rmu_sc0013812.1_g000001
rosa_roxburghii Rroxscaffold_3G00269810
rosa_rugosa Rorug06G0463800
rosa_samantha Rh7AG082100 Rh7BG065100 Rh7CG065500 Rh7DG065200
rosa_wichuraiana Rw7G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 298
Acc16I TGCGCA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 298
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 2 cut(s) 136, 345
AccII CGCG 3 cut(s) 9, 20, 554
AclWI GGATC 2 cut(s) 38, 89
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 2 cut(s) 473, 797
AcuI CTGAAG 1 cut(s) 766
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 5 cut(s) 329, 338, 545, 729, 787
AfiI CCNNNNNNNGG 5 cut(s) 72, 177, 324, 578, 590
AjnI CCWGG 1 cut(s) 465
AluBI AGCT 1 cut(s) 634
AluI AGCT 1 cut(s) 634
Alw26I GTCTC 3 cut(s) 342, 498, 588
AlwI GGATC 2 cut(s) 38, 89
AlwNI CAGNNNCTG 1 cut(s) 603
AoxI GGCC 5 cut(s) 58, 75, 468, 581, 702
ApeKI GCWGC 4 cut(s) 195, 367, 513, 756
ApoI RAATTY 2 cut(s) 473, 797
AspLEI GCGC 2 cut(s) 427, 812
AspS9I GGNCC 3 cut(s) 75, 398, 581
AsuC2I CCSGG 2 cut(s) 79, 319
AsuHPI GGTGA 4 cut(s) 175, 259, 391, 745
AvaII GGWCC 1 cut(s) 398
BalI TGGCCA 1 cut(s) 704
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 183, 258
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BauI CACGAG 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 145, 229
BbvI GCAGC 4 cut(s) 182, 379, 500, 743
BccI CCATC 2 cut(s) 77, 410
BceAI ACGGC 2 cut(s) 115, 508
BciT130I CCWGG 1 cut(s) 467
BcnI CCSGG 2 cut(s) 79, 319
BcoDI GTCTC 3 cut(s) 342, 498, 588
BfaI CTAG 1 cut(s) 176
BfoI RGCGCY 1 cut(s) 813
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 9 cut(s) 103, 148, 196, 368, 448, 514, 521, 757, 808
BlsI GCNGC 9 cut(s) 104, 149, 197, 369, 449, 515, 522, 758, 809
BmcAI AGTACT 1 cut(s) 787
Bme1390I CCNGG 3 cut(s) 79, 319, 467
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 75, 398, 581
BmiI GGNNCC 5 cut(s) 76, 106, 190, 399, 400
BmrFI CCNGG 3 cut(s) 79, 319, 467
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 354, 412
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 2 cut(s) 145, 229
BpuMI CCSGG 2 cut(s) 79, 319
Bsa29I ATCGAT 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 55, 97, 465
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 177, 324, 578, 590
Bse118I RCCGGY 2 cut(s) 185, 583
Bse1I ACTGG 3 cut(s) 365, 398, 735
Bse8I GATNNNNATC 1 cut(s) 740
BseBI CCWGG 1 cut(s) 467
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 3 cut(s) 55, 97, 465
BseGI GGATG 1 cut(s) 369
BseJI GATNNNNATC 1 cut(s) 740
BseLI CCNNNNNNNGG 5 cut(s) 72, 177, 324, 578, 590
BseNI ACTGG 3 cut(s) 365, 398, 735
BseRI GAGGAG 4 cut(s) 41, 44, 203, 678
BseXI GCAGC 4 cut(s) 182, 379, 500, 743
Bsh1236I CGCG 3 cut(s) 9, 20, 554
Bsh1285I CGRYCG 1 cut(s) 591
BshFI GGCC 5 cut(s) 60, 77, 470, 583, 704
BshNI GGYRCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 1 cut(s) 591
BsiSI CCGG 4 cut(s) 78, 186, 318, 584
BslFI GGGAC 2 cut(s) 154, 384
BslI CCNNNNNNNGG 5 cut(s) 72, 177, 324, 578, 590
BsmAI GTCTC 3 cut(s) 342, 498, 588
BsmFI GGGAC 2 cut(s) 154, 384
BsnI GGCC 5 cut(s) 60, 77, 470, 583, 704
Bsp1286I GDGCHC 2 cut(s) 183, 258
Bsp143I GATC 3 cut(s) 30, 81, 417
BspANI GGCC 5 cut(s) 60, 77, 470, 583, 704
BspDI ATCGAT 1 cut(s) 420
BspFNI CGCG 3 cut(s) 9, 20, 554
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 5 cut(s) 76, 106, 190, 399, 400
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 2 cut(s) 38, 89
BspT107I GGYRCC 1 cut(s) 104
BsrBI CCGCTC 2 cut(s) 136, 345
BsrFI RCCGGY 2 cut(s) 185, 583
BsrI ACTGG 3 cut(s) 365, 398, 735
BssAI RCCGGY 2 cut(s) 185, 583
BssECI CCNNGG 3 cut(s) 55, 97, 465
BssMI GATC 3 cut(s) 30, 81, 417
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 284
Bst2BI CACGAG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 2 cut(s) 271, 504
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 150
BstAPI GCANNNNNTGC 2 cut(s) 111, 364
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 518, 585, 672
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 369
BstFNI CGCG 3 cut(s) 9, 20, 554
BstH2I RGCGCY 1 cut(s) 813
BstHHI GCGC 2 cut(s) 427, 812
BstKTI GATC 3 cut(s) 33, 84, 420
BstMAI GTCTC 3 cut(s) 342, 498, 588
BstMBI GATC 3 cut(s) 30, 81, 417
BstMCI CGRYCG 1 cut(s) 591
BstMWI GCNNNNNNNGC 7 cut(s) 111, 195, 364, 631, 645, 654, 756
BstNI CCWGG 1 cut(s) 467
BstSCI CCNGG 3 cut(s) 77, 317, 465
BstUI CGCG 3 cut(s) 9, 20, 554
BstV1I GCAGC 4 cut(s) 182, 379, 500, 743
BstV2I GAAGAC 2 cut(s) 145, 229
BstX2I RGATCY 2 cut(s) 30, 81
BstYI RGATCY 2 cut(s) 30, 81
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 5 cut(s) 60, 77, 470, 583, 704
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 2 cut(s) 109, 695
BtsIMutI CAGTG 3 cut(s) 109, 358, 695
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 3 cut(s) 518, 585, 672
CaiI CAGNNNCTG 1 cut(s) 603
CciI TCATGA 1 cut(s) 457
CfoI GCGC 2 cut(s) 427, 812
Cfr10I RCCGGY 2 cut(s) 185, 583
Cfr13I GGNCC 3 cut(s) 75, 398, 581
ClaI ATCGAT 1 cut(s) 420
CseI GACGC 2 cut(s) 139, 739
Csp6I GTAC 5 cut(s) 328, 337, 544, 728, 786
CviAII CATG 1 cut(s) 458
CviQI GTAC 5 cut(s) 328, 337, 544, 728, 786
DpnI GATC 3 cut(s) 32, 83, 419
DpnII GATC 3 cut(s) 30, 81, 417
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco24I GRGCYC 2 cut(s) 183, 258
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 465
EcoT38I GRGCYC 2 cut(s) 183, 258
FaeI CATG 1 cut(s) 461
FaiI YATR 3 cut(s) 459, 500, 707
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 473, 505
FaqI GGGAC 2 cut(s) 154, 384
FatI CATG 1 cut(s) 457
Fnu4HI GCNGC 9 cut(s) 103, 148, 196, 368, 448, 514, 521, 757, 808
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 2 cut(s) 183, 258
Fsp4HI GCNGC 9 cut(s) 103, 148, 196, 368, 448, 514, 521, 757, 808
FspBI CTAG 1 cut(s) 176
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 2 cut(s) 426, 811
GluI GCNGC 9 cut(s) 103, 148, 196, 368, 448, 514, 521, 757, 808
HaeII RGCGCY 1 cut(s) 813
HaeIII GGCC 5 cut(s) 60, 77, 470, 583, 704
HapII CCGG 4 cut(s) 78, 186, 318, 584
HgaI GACGC 2 cut(s) 139, 739
HhaI GCGC 2 cut(s) 427, 812
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 461
Hin6I GCGC 2 cut(s) 425, 810
HinP1I GCGC 2 cut(s) 425, 810
HinfI GANTC 6 cut(s) 201, 439, 461, 537, 650, 736
HpaII CCGG 4 cut(s) 78, 186, 318, 584
HphI GGTGA 4 cut(s) 175, 259, 391, 745
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 2 cut(s) 573, 593
Hpy188III TCNNGA 4 cut(s) 286, 406, 458, 682
Hpy8I GTNNAC 1 cut(s) 267
Hpy99I CGWCG 3 cut(s) 455, 559, 755
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 2 cut(s) 271, 504
HpyCH4IV ACGT 4 cut(s) 375, 453, 557, 611
HpyCH4V TGCA 5 cut(s) 367, 435, 516, 606, 657
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 195, 364, 631, 645, 654, 756
HpySE526I ACGT 4 cut(s) 375, 453, 557, 611
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 461
HspAI GCGC 2 cut(s) 425, 810
KflI GGGWCCC 1 cut(s) 398
KroI GCCGGC 1 cut(s) 583
KroNI GCCGGC 1 cut(s) 585
Kzo9I GATC 3 cut(s) 30, 81, 417
LmnI GCTCC 2 cut(s) 194, 615
Lsp1109I GCAGC 4 cut(s) 182, 379, 500, 743
LweI GCATC 2 cut(s) 354, 412
MaeI CTAG 1 cut(s) 176
MaeII ACGT 4 cut(s) 375, 453, 557, 611
MaeIII GTNAC 1 cut(s) 286
MalI GATC 3 cut(s) 32, 83, 419
MbiI CCGCTC 2 cut(s) 136, 345
MboI GATC 3 cut(s) 30, 81, 417
MboII GAAGA 5 cut(s) 37, 145, 148, 229, 640
MflI RGATCY 2 cut(s) 30, 81
MhlI GDGCHC 2 cut(s) 183, 258
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 2 cut(s) 473, 797
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 3 cut(s) 195, 448, 659
MmeI TCCRAC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 704
MroNI GCCGGC 1 cut(s) 583
MscI TGGCCA 1 cut(s) 704
MseI TTAA 3 cut(s) 35, 227, 711
MslI CAYNNNNRTG 1 cut(s) 284
Msp20I TGGCCA 1 cut(s) 704
MspI CCGG 4 cut(s) 78, 186, 318, 584
MspR9I CCNGG 3 cut(s) 79, 319, 467
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 3 cut(s) 9, 20, 554
MwoI GCNNNNNNNGC 7 cut(s) 111, 195, 364, 631, 645, 654, 756
NaeI GCCGGC 1 cut(s) 585
NciI CCSGG 2 cut(s) 79, 319
NdeII GATC 3 cut(s) 30, 81, 417
NgoMIV GCCGGC 1 cut(s) 583
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 5 cut(s) 76, 106, 190, 399, 400
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 286
NsbI TGCGCA 1 cut(s) 426
PagI TCATGA 1 cut(s) 457
PaqCI CACCTGC 1 cut(s) 298
PcsI WCGNNNNNNNCGW 5 cut(s) 237, 258, 267, 282, 597
PdiI GCCGGC 1 cut(s) 585
PfeI GAWTC 3 cut(s) 461, 537, 736
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 9 cut(s) 104, 149, 197, 369, 449, 515, 522, 758, 809
PleI GAGTC 3 cut(s) 195, 447, 658
PpsI GAGTC 3 cut(s) 195, 447, 658
Ppu21I YACGTR 1 cut(s) 376
PpuMI RGGWCCY 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 5 cut(s) 76, 106, 190, 399, 400
PspPI GGNCC 3 cut(s) 75, 398, 581
PspPPI RGGWCCY 1 cut(s) 398
PstNI CAGNNNCTG 1 cut(s) 603
PsuI RGATCY 2 cut(s) 30, 81
RsaI GTAC 5 cut(s) 329, 338, 545, 729, 787
RsaNI GTAC 5 cut(s) 328, 337, 544, 728, 786
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 3 cut(s) 35, 227, 711
SatI GCNGC 9 cut(s) 103, 148, 196, 368, 448, 514, 521, 757, 808
Sau3AI GATC 3 cut(s) 30, 81, 417
Sau96I GGNCC 3 cut(s) 75, 398, 581
ScaI AGTACT 1 cut(s) 787
SchI GAGTC 3 cut(s) 195, 448, 659
ScrFI CCNGG 3 cut(s) 79, 319, 467
SduI GDGCHC 2 cut(s) 183, 258
SetI ASST 6 cut(s) 312, 378, 456, 560, 614, 636
SfaNI GCATC 2 cut(s) 354, 412
SinI GGWCC 1 cut(s) 398
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 2 cut(s) 473, 797
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 3 cut(s) 77, 317, 465
TaaI ACNGT 2 cut(s) 271, 504
TaiI ACGT 4 cut(s) 378, 456, 560, 614
TaqI TCGA 2 cut(s) 420, 540
TasI AATT 2 cut(s) 473, 797
TatI WGTACW 4 cut(s) 336, 543, 727, 785
TauI GCSGC 5 cut(s) 105, 150, 450, 523, 810
TfiI GAWTC 3 cut(s) 461, 537, 736
Tru1I TTAA 3 cut(s) 35, 227, 711
Tru9I TTAA 3 cut(s) 35, 227, 711
TscAI CASTG 3 cut(s) 116, 365, 695
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 4 cut(s) 195, 367, 513, 756
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 446, 474
TspRI CASTG 3 cut(s) 116, 365, 695
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 2 cut(s) 473, 797
XspI CTAG 1 cut(s) 176
ZrmI AGTACT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.