Rroxscaffold_3G00269810

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013325
Physical Location & Seq
Reverse (-)
62563435 .. 62564905
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269810.1

Sequence Viewer

Length: 750 bp
ATGTTACGCATAAGCAACACCGTGATCGGGATTCTGAATGCGATGTTCCTCCTCCTGGGACTCGCCTCCATCGGCGCATCGATCTACTTCCACCTCCACGGCGGGACAGTCTGCCAGAAGGTCCTCCAGAACCCTCTCCTCTTCGCCGGACTCTTCTTCGTTGTCGTGTCCCTTCTCGGCCTCGCCGGATCGTGCTGCCGACTCAACTCTCTCCTCTACACCTACCTCGTCTTAATCTTTCTCCTCATCCTCGGACTCATCGCGTTCACCGTGTTCGCGCTTTTCGTGACCAACAAGGGGATCGGACAGGCCGTGTCGGGACGAGGGTACAAGGAGTACAAGGTTGGAGACTTCTCGCATTGGCTCCAGCATTACGTTGTTAATGGGAAGAACTGGGAGCCGATCAAGAGTTGTCTCATTGATGCTCAGGTTTGCCAGAGTCTCGGCGGCGATGTTCATCAGACCAAGTCCGATTTTTACAAGAAGAACTTGTCTCCGATTCAGTCGGGACCTAAAGAGCCGGACAGTGATTGCATGACTTGGAGCAACAAGCAGAACAAGCTGTGTTATGACTGCAAGTCATGCAAAGGAGGAATGCTGGCGAACATTCGCAAAGAGTGGAGGTCCCTGGCCATACTCAACTGCTGTATTCTCGTTCTCCTTACCCTCGTCTACTGCGTTGGCTGCTGTGCGACCAAGAACAATCGCTTCAGTACTTACAATTATAACAAGTACAGAGGAGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

249

Amino Acids

27.57

Weight (kDa)

9.16

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 231 7.4e-25 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18520
fragaria_vesca FvH4_5g12970
malus_domestica MD06G1208400.v1.1
prunus_persica Prupe.5G212900_v2.0.a1
pyrus_communis pycom06g18620
rosa_chinensis RchiOBHm_Chr7g0184191
rosa_laevigata RLG00000004995
rosa_multiflora Rmu_co8233853.1_g000001 Rmu_co8323147.1_g000001 Rmu_sc0012221.1_g000007 Rmu_sc0013812.1_g000001
rosa_roxburghii Rroxscaffold_3G00269810
rosa_rugosa Rorug06G0463800
rosa_samantha Rh7AG082100 Rh7BG065100 Rh7CG065500 Rh7DG065200
rosa_wichuraiana Rw7G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccI GTMKAC 1 cut(s) 672
AccII CGCG 2 cut(s) 263, 278
AciI CCGC 2 cut(s) 102, 447
AclWI GGATC 2 cut(s) 196, 308
AcoI YGGCCR 1 cut(s) 630
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 4 cut(s) 329, 338, 715, 734
AfiI CCNNNNNNNGG 3 cut(s) 27, 55, 297
AhdI GACNNNNNGTC 1 cut(s) 577
AjnI CCWGG 2 cut(s) 54, 627
AluBI AGCT 1 cut(s) 562
AluI AGCT 1 cut(s) 562
Alw26I GTCTC 4 cut(s) 342, 419, 446, 498
AlwI GGATC 2 cut(s) 196, 308
AoxI GGCC 3 cut(s) 178, 309, 630
ApeKI GCWGC 2 cut(s) 195, 684
AspLEI GCGC 3 cut(s) 77, 280, 746
AspS9I GGNCC 3 cut(s) 121, 509, 624
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 3 cut(s) 121, 509, 624
BalI TGGCCA 1 cut(s) 632
BbvI GCAGC 2 cut(s) 182, 671
BccI CCATC 1 cut(s) 77
BceAI ACGGC 2 cut(s) 115, 296
BciT130I CCWGG 2 cut(s) 56, 629
BcoDI GTCTC 4 cut(s) 342, 419, 446, 498
BfoI RGCGCY 1 cut(s) 747
BisI GCNGC 3 cut(s) 196, 448, 685
BlsI GCNGC 3 cut(s) 197, 449, 686
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 2 cut(s) 56, 629
Bme18I GGWCC 3 cut(s) 121, 509, 624
BmeRI GACNNNNNGTC 1 cut(s) 577
BmgT120I GGNCC 3 cut(s) 121, 509, 624
BmiI GGNNCC 4 cut(s) 365, 399, 510, 626
BmrFI CCNGG 2 cut(s) 56, 629
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 86, 412
BmuI ACTGGG 1 cut(s) 403
BpmI CTGGAG 2 cut(s) 110, 350
Bpu10I CCTNAGC 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 4 cut(s) 55, 97, 250, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 55, 297
Bse1I ACTGG 1 cut(s) 398
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 2 cut(s) 56, 629
BseCI ATCGAT 1 cut(s) 80
BseDI CCNNGG 4 cut(s) 55, 97, 250, 627
BseGI GGATG 1 cut(s) 246
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 27, 55, 297
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 4 cut(s) 41, 128, 203, 233
BseXI GCAGC 2 cut(s) 182, 671
Bsh1236I CGCG 2 cut(s) 263, 278
BshFI GGCC 3 cut(s) 180, 311, 632
BshVI ATCGAT 1 cut(s) 80
BsiSI CCGG 3 cut(s) 147, 186, 521
BslFI GGGAC 6 cut(s) 72, 118, 154, 333, 522, 610
BslI CCNNNNNNNGG 3 cut(s) 27, 55, 297
BsmAI GTCTC 4 cut(s) 342, 419, 446, 498
BsmFI GGGAC 6 cut(s) 72, 118, 154, 333, 522, 610
BsmI GAATGC 2 cut(s) 43, 600
BsnI GGCC 3 cut(s) 180, 311, 632
Bsp143I GATC 5 cut(s) 24, 81, 188, 300, 402
BspACI CCGC 2 cut(s) 102, 447
BspANI GGCC 3 cut(s) 180, 311, 632
BspCNI CTCAG 1 cut(s) 439
BspDI ATCGAT 1 cut(s) 80
BspFNI CGCG 2 cut(s) 263, 278
BspLI GGNNCC 4 cut(s) 365, 399, 510, 626
BspPI GGATC 2 cut(s) 196, 308
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 4 cut(s) 55, 97, 250, 627
BssMI GATC 5 cut(s) 24, 81, 188, 300, 402
Bst2UI CCWGG 2 cut(s) 56, 629
Bst4CI ACNGT 4 cut(s) 22, 109, 271, 527
Bst6I CTCTTC 2 cut(s) 146, 158
BstAPI GCANNNNNTGC 1 cut(s) 582
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 2 cut(s) 263, 278
BstH2I RGCGCY 1 cut(s) 747
BstHHI GCGC 3 cut(s) 77, 280, 746
BstKTI GATC 5 cut(s) 27, 84, 191, 303, 405
BstMAI GTCTC 4 cut(s) 342, 419, 446, 498
BstMBI GATC 5 cut(s) 24, 81, 188, 300, 402
BstMWI GCNNNNNNNGC 3 cut(s) 559, 582, 684
BstNI CCWGG 2 cut(s) 56, 629
BstSCI CCNGG 2 cut(s) 54, 627
BstUI CGCG 2 cut(s) 263, 278
BstV1I GCAGC 2 cut(s) 182, 671
Bsu15I ATCGAT 1 cut(s) 80
BsuRI GGCC 3 cut(s) 180, 311, 632
BsuTUI ATCGAT 1 cut(s) 80
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 3 cut(s) 56, 244, 465
BtsCI GGATG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 1 cut(s) 600
CfoI GCGC 3 cut(s) 77, 280, 746
Cfr13I GGNCC 3 cut(s) 121, 509, 624
ClaI ATCGAT 1 cut(s) 80
Csp6I GTAC 4 cut(s) 328, 337, 714, 733
CviAII CATG 2 cut(s) 535, 582
CviJI RGCY 8 cut(s) 180, 311, 364, 400, 520, 562, 632, 684
CviKI_1 RGCY 8 cut(s) 180, 311, 364, 400, 520, 562, 632, 684
CviQI GTAC 4 cut(s) 328, 337, 714, 733
DdeI CTNAG 1 cut(s) 426
DpnI GATC 5 cut(s) 26, 83, 190, 302, 404
DpnII GATC 5 cut(s) 24, 81, 188, 300, 402
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 146, 158
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 2 cut(s) 146, 158
Eco47I GGWCC 3 cut(s) 121, 509, 624
Eco57I CTGAAG 1 cut(s) 694
EcoO109I RGGNCCY 3 cut(s) 121, 509, 624
EcoRII CCWGG 2 cut(s) 54, 627
FaeI CATG 2 cut(s) 538, 585
FaiI YATR 6 cut(s) 11, 536, 570, 583, 635, 726
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 6 cut(s) 72, 118, 154, 333, 522, 610
FatI CATG 2 cut(s) 534, 581
FauI CCCGC 1 cut(s) 95
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 3 cut(s) 196, 448, 685
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 3 cut(s) 196, 448, 685
GlaI GCGC 3 cut(s) 76, 279, 745
GluI GCNGC 3 cut(s) 196, 448, 685
GsuI CTGGAG 2 cut(s) 110, 350
HaeII RGCGCY 1 cut(s) 747
HaeIII GGCC 3 cut(s) 180, 311, 632
HapII CCGG 3 cut(s) 147, 186, 521
HhaI GCGC 3 cut(s) 77, 280, 746
Hin1II CATG 2 cut(s) 538, 585
Hin6I GCGC 3 cut(s) 75, 278, 744
HinP1I GCGC 3 cut(s) 75, 278, 744
HinfI GANTC 7 cut(s) 31, 60, 150, 201, 255, 439, 499
HpaII CCGG 3 cut(s) 147, 186, 521
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 267, 673
Hpy188I TCNGA 6 cut(s) 36, 254, 305, 462, 472, 498
Hpy188III TCNNGA 6 cut(s) 28, 127, 286, 318, 406, 507
Hpy8I GTNNAC 2 cut(s) 267, 673
HpyAV CCTTC 2 cut(s) 112, 182
HpyCH4III ACNGT 4 cut(s) 22, 109, 271, 527
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 3 cut(s) 534, 576, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 559, 582, 684
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 2 cut(s) 538, 585
HspAI GCGC 3 cut(s) 75, 278, 744
Kzo9I GATC 5 cut(s) 24, 81, 188, 300, 402
LmnI GCTCC 3 cut(s) 369, 397, 543
Lsp1109I GCAGC 2 cut(s) 182, 671
LweI GCATC 2 cut(s) 86, 412
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 3, 286
MalI GATC 5 cut(s) 26, 83, 190, 302, 404
MboI GATC 5 cut(s) 24, 81, 188, 300, 402
MboII GAAGA 5 cut(s) 133, 145, 148, 400, 496
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 1 cut(s) 721
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 5 cut(s) 54, 144, 195, 249, 448
MmeI TCCRAC 1 cut(s) 325
Mox20I TGGCCA 1 cut(s) 632
MscI TGGCCA 1 cut(s) 632
MseI TTAA 2 cut(s) 233, 381
Msp20I TGGCCA 1 cut(s) 632
MspI CCGG 3 cut(s) 147, 186, 521
MspR9I CCNGG 2 cut(s) 56, 629
Mva1269I GAATGC 2 cut(s) 43, 600
MvaI CCWGG 2 cut(s) 56, 629
MvnI CGCG 2 cut(s) 263, 278
MwoI GCNNNNNNNGC 3 cut(s) 559, 582, 684
NdeII GATC 5 cut(s) 24, 81, 188, 300, 402
NlaIII CATG 2 cut(s) 538, 585
NlaIV GGNNCC 4 cut(s) 365, 399, 510, 626
NmeAIII GCCGAG 2 cut(s) 156, 423
NmuCI GTSAC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 4 cut(s) 267, 282, 309, 675
PctI GAATGC 2 cut(s) 43, 600
PfeI GAWTC 2 cut(s) 31, 499
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 3 cut(s) 197, 449, 686
PleI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpsI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpuMI RGGWCCY 3 cut(s) 121, 509, 624
PsiI TTATAA 1 cut(s) 726
Psp5II RGGWCCY 3 cut(s) 121, 509, 624
Psp6I CCWGG 2 cut(s) 54, 627
PspGI CCWGG 2 cut(s) 54, 627
PspN4I GGNNCC 4 cut(s) 365, 399, 510, 626
PspPI GGNCC 3 cut(s) 121, 509, 624
PspPPI RGGWCCY 3 cut(s) 121, 509, 624
PsyI GACNNNGTC 1 cut(s) 466
RsaI GTAC 4 cut(s) 329, 338, 715, 734
RsaNI GTAC 4 cut(s) 328, 337, 714, 733
SaqAI TTAA 2 cut(s) 233, 381
SatI GCNGC 3 cut(s) 196, 448, 685
Sau3AI GATC 5 cut(s) 24, 81, 188, 300, 402
Sau96I GGNCC 3 cut(s) 121, 509, 624
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 5 cut(s) 54, 144, 195, 249, 448
ScrFI CCNGG 2 cut(s) 56, 629
SfaNI GCATC 2 cut(s) 86, 412
SinI GGWCC 3 cut(s) 121, 509, 624
Sse9I AATT 1 cut(s) 721
SsiI CCGC 2 cut(s) 102, 447
StyD4I CCNGG 2 cut(s) 54, 627
TaaI ACNGT 4 cut(s) 22, 109, 271, 527
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 80
TasI AATT 1 cut(s) 721
TatI WGTACW 3 cut(s) 336, 713, 732
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 2 cut(s) 31, 499
Tru1I TTAA 2 cut(s) 233, 381
Tru9I TTAA 2 cut(s) 233, 381
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 2 cut(s) 195, 684
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 446
TspRI CASTG 1 cut(s) 532
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 3 cut(s) 121, 509, 624
XmiI GTMKAC 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.