Rmu_sc0012221.1_g000007

Tetraspanin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012221.1
OG0013325
Physical Location & Seq
Forward (+)
27102 .. 28535
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012221.1_g000007.1.cds

Sequence Viewer

Length: 777 bp
atgttacgcataagcaacaccgtgatcgggatcctcaatgcgatgttcctcctcctgggactcgcctccatcggcgcgtcgatctacttccacctccacggcggcacagtctgccagaaggtcctccagaaccctctcctcttcgccggactcttcttcgttgtcgtgtccgtcctcggcctcgccggatcgtgctgccgactcaactctctcctctacacctacctcgtcttaatctttctcctcatcctcggactcatcgcgttcaccgtgttcgcgcttttcgtgaccaacaaggggatcggacaggccgtgtcgggacgagggtacaaggagtacaaggttggagacttctcgcattggctccagcattacgttgttaatgggaagaactgggagccgatcaagagttgtctcattgatgctcaggtttgccagagtctcggcggcgatgttcatcagaccaagtccgatttttacaagaagaacttgtctccgattcagaatgcaacatattgggttgttccaaagtcgggacctaaagagccggacagtgattgcatgacttggagcaacaaacagaacaagttgtgttatgactgcaagtcatgcaaaggaggaatgctggcgaacattcgcaaagagtggaggtctctggccatactcaactgctgtattctcgttctccttaccctcgtctactgcgttggttgctgtgcgaccaagaacaatcgcttcagtacttacaattacaacaagtacagaggaggcgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

258

Amino Acids

28.62

Weight (kDa)

9.21

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18520
fragaria_vesca FvH4_5g12970
malus_domestica MD06G1208400.v1.1
prunus_persica Prupe.5G212900_v2.0.a1
pyrus_communis pycom06g18620
rosa_chinensis RchiOBHm_Chr7g0184191
rosa_laevigata RLG00000004995
rosa_multiflora Rmu_co8233853.1_g000001 Rmu_co8323147.1_g000001 Rmu_sc0012221.1_g000007 Rmu_sc0013812.1_g000001
rosa_roxburghii Rroxscaffold_3G00269810
rosa_rugosa Rorug06G0463800
rosa_samantha Rh7AG082100 Rh7BG065100 Rh7CG065500 Rh7DG065200
rosa_wichuraiana Rw7G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 699
AccII CGCG 3 cut(s) 77, 263, 278
AciI CCGC 2 cut(s) 102, 447
AclWI GGATC 4 cut(s) 25, 38, 196, 308
AcoI YGGCCR 1 cut(s) 657
AcuI CTGAAG 1 cut(s) 721
AfaI GTAC 4 cut(s) 329, 338, 742, 761
AfiI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 533
AhdI GACNNNNNGTC 1 cut(s) 604
AjnI CCWGG 1 cut(s) 54
Alw26I GTCTC 5 cut(s) 342, 419, 446, 498, 657
AlwI GGATC 4 cut(s) 25, 38, 196, 308
AoxI GGCC 3 cut(s) 178, 309, 657
ApeKI GCWGC 1 cut(s) 195
AspLEI GCGC 3 cut(s) 77, 280, 773
AspS9I GGNCC 2 cut(s) 121, 536
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 121, 536
BalI TGGCCA 1 cut(s) 659
BamHI GGATCC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 77
BceAI ACGGC 2 cut(s) 115, 296
BciT130I CCWGG 1 cut(s) 56
BcoDI GTCTC 5 cut(s) 342, 419, 446, 498, 657
BfoI RGCGCY 1 cut(s) 774
BisI GCNGC 3 cut(s) 103, 196, 448
BlsI GCNGC 3 cut(s) 104, 197, 449
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 2 cut(s) 121, 536
BmeRI GACNNNNNGTC 1 cut(s) 604
BmgT120I GGNCC 2 cut(s) 121, 536
BmiI GGNNCC 4 cut(s) 32, 365, 399, 537
BmrFI CCNGG 1 cut(s) 56
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 403
BpmI CTGGAG 2 cut(s) 110, 350
Bpu10I CCTNAGC 1 cut(s) 426
BsaBI GATNNNNATC 2 cut(s) 29, 456
BsaI GGTCTC 1 cut(s) 657
BsaJI CCNNGG 4 cut(s) 55, 97, 175, 250
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 55, 297, 533
Bse1I ACTGG 1 cut(s) 398
Bse8I GATNNNNATC 2 cut(s) 29, 456
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 4 cut(s) 55, 97, 175, 250
BseGI GGATG 1 cut(s) 246
BseJI GATNNNNATC 2 cut(s) 29, 456
BseLI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 533
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 4 cut(s) 41, 128, 203, 233
BseXI GCAGC 1 cut(s) 182
Bsh1236I CGCG 3 cut(s) 77, 263, 278
BshFI GGCC 3 cut(s) 180, 311, 659
BsiSI CCGG 3 cut(s) 147, 186, 548
BslFI GGGAC 3 cut(s) 72, 333, 549
BslI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 533
BsmAI GTCTC 5 cut(s) 342, 419, 446, 498, 657
BsmFI GGGAC 3 cut(s) 72, 333, 549
BsmI GAATGC 2 cut(s) 511, 627
BsnI GGCC 3 cut(s) 180, 311, 659
Bso31I GGTCTC 1 cut(s) 657
Bsp143I GATC 6 cut(s) 24, 30, 81, 188, 300, 402
BspACI CCGC 2 cut(s) 102, 447
BspANI GGCC 3 cut(s) 180, 311, 659
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 3 cut(s) 77, 263, 278
BspLI GGNNCC 4 cut(s) 32, 365, 399, 537
BspPI GGATC 4 cut(s) 25, 38, 196, 308
BspTNI GGTCTC 1 cut(s) 657
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 4 cut(s) 55, 97, 175, 250
BssMI GATC 6 cut(s) 24, 30, 81, 188, 300, 402
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 4 cut(s) 22, 109, 271, 554
Bst6I CTCTTC 2 cut(s) 146, 158
BstAPI GCANNNNNTGC 2 cut(s) 111, 609
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 3 cut(s) 77, 263, 278
BstH2I RGCGCY 1 cut(s) 774
BstHHI GCGC 3 cut(s) 77, 280, 773
BstKTI GATC 6 cut(s) 27, 33, 84, 191, 303, 405
BstMAI GTCTC 5 cut(s) 342, 419, 446, 498, 657
BstMBI GATC 6 cut(s) 24, 30, 81, 188, 300, 402
BstMWI GCNNNNNNNGC 3 cut(s) 111, 609, 711
BstNI CCWGG 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 54
BstUI CGCG 3 cut(s) 77, 263, 278
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 3 cut(s) 180, 311, 659
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 3 cut(s) 56, 244, 465
BtsCI GGATG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 1 cut(s) 627
CfoI GCGC 3 cut(s) 77, 280, 773
Cfr13I GGNCC 2 cut(s) 121, 536
CseI GACGC 1 cut(s) 66
Csp6I GTAC 4 cut(s) 328, 337, 741, 760
CviAII CATG 2 cut(s) 562, 609
CviJI RGCY 6 cut(s) 180, 311, 364, 400, 547, 659
CviKI_1 RGCY 6 cut(s) 180, 311, 364, 400, 547, 659
CviQI GTAC 4 cut(s) 328, 337, 741, 760
DdeI CTNAG 1 cut(s) 426
DpnI GATC 6 cut(s) 26, 32, 83, 190, 302, 404
DpnII GATC 6 cut(s) 24, 30, 81, 188, 300, 402
DriI GACNNNNNGTC 1 cut(s) 604
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 2 cut(s) 146, 158
Eam1105I GACNNNNNGTC 1 cut(s) 604
EarI CTCTTC 2 cut(s) 146, 158
Eco31I GGTCTC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 121, 536
Eco57I CTGAAG 1 cut(s) 721
EcoO109I RGGNCCY 2 cut(s) 121, 536
EcoRII CCWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 565, 612
FaiI YATR 6 cut(s) 11, 514, 563, 597, 610, 662
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 3 cut(s) 72, 333, 549
FatI CATG 2 cut(s) 561, 608
FblI GTMKAC 1 cut(s) 699
Fnu4HI GCNGC 3 cut(s) 103, 196, 448
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 3 cut(s) 103, 196, 448
GlaI GCGC 3 cut(s) 76, 279, 772
GluI GCNGC 3 cut(s) 103, 196, 448
GsuI CTGGAG 2 cut(s) 110, 350
HaeII RGCGCY 1 cut(s) 774
HaeIII GGCC 3 cut(s) 180, 311, 659
HapII CCGG 3 cut(s) 147, 186, 548
HgaI GACGC 1 cut(s) 66
HhaI GCGC 3 cut(s) 77, 280, 773
Hin1II CATG 2 cut(s) 565, 612
Hin6I GCGC 3 cut(s) 75, 278, 771
HinP1I GCGC 3 cut(s) 75, 278, 771
HinfI GANTC 6 cut(s) 60, 150, 201, 255, 439, 499
HpaII CCGG 3 cut(s) 147, 186, 548
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 267, 700
Hpy188I TCNGA 6 cut(s) 254, 305, 462, 472, 498, 504
Hpy188III TCNNGA 6 cut(s) 28, 127, 286, 318, 406, 534
Hpy8I GTNNAC 2 cut(s) 267, 700
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 4 cut(s) 22, 109, 271, 554
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 4 cut(s) 509, 561, 603, 612
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 609, 711
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 2 cut(s) 565, 612
HspAI GCGC 3 cut(s) 75, 278, 771
Kzo9I GATC 6 cut(s) 24, 30, 81, 188, 300, 402
LmnI GCTCC 3 cut(s) 369, 397, 570
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 412
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 3, 286
MalI GATC 6 cut(s) 26, 32, 83, 190, 302, 404
MboI GATC 6 cut(s) 24, 30, 81, 188, 300, 402
MboII GAAGA 5 cut(s) 133, 145, 148, 400, 496
MflI RGATCY 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 1 cut(s) 748
MluNI TGGCCA 1 cut(s) 659
MlyI GAGTC 5 cut(s) 54, 144, 195, 249, 448
MmeI TCCRAC 1 cut(s) 325
Mox20I TGGCCA 1 cut(s) 659
MscI TGGCCA 1 cut(s) 659
MseI TTAA 3 cut(s) 233, 381, 775
Msp20I TGGCCA 1 cut(s) 659
MspI CCGG 3 cut(s) 147, 186, 548
MspR9I CCNGG 1 cut(s) 56
Mva1269I GAATGC 2 cut(s) 511, 627
MvaI CCWGG 1 cut(s) 56
MvnI CGCG 3 cut(s) 77, 263, 278
MwoI GCNNNNNNNGC 3 cut(s) 111, 609, 711
NdeII GATC 6 cut(s) 24, 30, 81, 188, 300, 402
NlaIII CATG 2 cut(s) 565, 612
NlaIV GGNNCC 4 cut(s) 32, 365, 399, 537
NmeAIII GCCGAG 2 cut(s) 156, 423
NmuCI GTSAC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 4 cut(s) 267, 282, 309, 702
PctI GAATGC 2 cut(s) 511, 627
PfeI GAWTC 1 cut(s) 499
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 3 cut(s) 104, 197, 449
PleI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpsI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpuMI RGGWCCY 2 cut(s) 121, 536
Psp5II RGGWCCY 2 cut(s) 121, 536
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 32, 365, 399, 537
PspPI GGNCC 2 cut(s) 121, 536
PspPPI RGGWCCY 2 cut(s) 121, 536
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 466
RsaI GTAC 4 cut(s) 329, 338, 742, 761
RsaNI GTAC 4 cut(s) 328, 337, 741, 760
SaqAI TTAA 3 cut(s) 233, 381, 775
SatI GCNGC 3 cut(s) 103, 196, 448
Sau3AI GATC 6 cut(s) 24, 30, 81, 188, 300, 402
Sau96I GGNCC 2 cut(s) 121, 536
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 5 cut(s) 54, 144, 195, 249, 448
ScrFI CCNGG 1 cut(s) 56
SetI ASST 9 cut(s) 96, 123, 224, 228, 345, 378, 432, 541, 653
SfaNI GCATC 1 cut(s) 412
SinI GGWCC 2 cut(s) 121, 536
Sse9I AATT 1 cut(s) 748
SsiI CCGC 2 cut(s) 102, 447
StyD4I CCNGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 22, 109, 271, 554
TaiI ACGT 1 cut(s) 378
TaqI TCGA 1 cut(s) 80
TasI AATT 1 cut(s) 748
TatI WGTACW 3 cut(s) 336, 740, 759
TauI GCSGC 2 cut(s) 105, 450
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 3 cut(s) 233, 381, 775
Tru9I TTAA 3 cut(s) 233, 381, 775
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 446
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 559
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 121, 536
XmiI GTMKAC 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.