Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011653

Physical Location & Seq

Genomic Coordinates Reverse (-)

6937719 .. 6940832

Gene Structure 3114 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G20010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAGAGAGATCCTTCACATTCAAGGTGGTCAATGCGGTAACCAGATTGGTTCCAAGTTCTGGGAAGTCATCTGCGACGAGCACGGCATCGATTCCACCGGACGTTACAGTGGAGACACTGCAGATCTCCAGCTTGAACGTATCAATGTCTATTACAATGAAGCTTCAGGTGGAAGATACGTTCCTCGTGCTGTTCTTATGGATCTTGAGCCTGGTACTATGGATAGTATCAGATCCGGACCGTTTGGTCAGATCTTCCGTCCTGATAACTTTGTCTTTGGTCAGTCTGGTGCTGGTAATAATTGGGCTAAAGGTCATTACACTGAAGGTGCTGAACTTATTGATGCTGTTCTTGATGTTGTTCGTAAAGAAGCTGAGAACTGCGATTGCCTTCAAGGGTTTCAGGTGTGCCATTCGTTGGGAGGAGGCACAGGTTCAGGAATGGGAACACTATTGATATCGAAGATCCGAGAGGAGTATCCAGACAGAATGATGCTCACTTTCTCTGTTTTCCCATCTCCTAAAGTCTCTGACACAGTCGTTGAGCCTTACAATGCAACTCTCTCTGTCCATCAGCTTGTAGAGAACGCTGATGAATGCATGGTCCTCGACAATGAAGCTCTCTACGATATCTGCTTCCGTACCCTCAAACTCAGCACTCCTAGCTTTGGAGACTTGAACCATTTGATCTCCGCAACAATGAGCGGTGTGACTTGCTCCTTAAGGTTCCCCGGACAACTAAACTCTGACCTCAGGAAACTCGCGGTGAACCTAATCCCATTCCCTCGTCTCCATTTCTTCATGGTGGGTTTTGCGCCCCTCACTTCCCGTGGCTCGCAACAATACATCTCACTCACCGTTCCTGAGCTGACTCAACAAATGTGGGACTCCAAAAACATGATGTGTGCAGCTGATCCACGTCACGGACGTTACCTCACAGCCTCTGCTATTTTCAGAGGACAGATGAGCACAAAAGAAGTCGACGAGCAAATCCTAAACATACAGAACAAGAACTCGTCTTACTTCGTTGAGTGGATCCCAAACAACGTGAAATCCAGCGTTTGTGATATCCCACCAAAGGGGCTCAAAATGGCGGCGACGTTTGTGGGTAACTCGACGTCGATTCAAGAAATGTTCAGGAGAGTGAGTGAGCAGTTCACGGCTATGTTTAGGCGTAAAGCGTTTCTGCATTGGTACACAGGAGAAGGAATGGACGAAATGGAGTTTACGGAAGCTGAGAGCAATATGAATGATTTGGTCGCTGAATACCAACAGTACCAAGATGCTACTGCTGATGAGGAAGGTGAGTATGATGTTGAAGAAGAAGAGGAGGGAGATTATGAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005856 GO:0005886 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009653 GO:0009826 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0030312 GO:0031090 GO:0032502 GO:0032989 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045298 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071840 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

449

Amino Acids

50.34

Weight (kDa)

4.66

Isoelectric Point (pI)

38.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 212	3.8e-65	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	262 - 383	8.9e-40	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20010 AT1G75780
fragaria_vesca	FvH4_2g38400 FvH4_2g38400
malus_domestica	MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica	Prupe.1G364800_v2.0.a1
pyrus_communis	pycom08g01090 pycom15g01040
rosa_chinensis	RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata	RLG00000011125
rosa_multiflora	Rmu_ssc0000460.1_g000016
rosa_rugosa	Rorug06G0318000
rosa_samantha	Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana	Rw6G037410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	246
AatII	GACGTC	1 cut(s)	1121
AccB7I	CCANNNNNTGG	2 cut(s)	60, 418
AccBSI	CCGCTC	1 cut(s)	705
AccI	GTMKAC	1 cut(s)	981
AccII	CGCG	1 cut(s)	764
AccIII	TCCGGA	1 cut(s)	236
AciI	CCGC	5 cut(s)	36, 693, 705, 764, 1094
AclWI	GGATC	7 cut(s)	4, 210, 228, 460, 908, 1030, 1043
AcuI	CTGAAG	2 cut(s)	150, 345
AcyI	GRCGYC	1 cut(s)	1118
AfaI	GTAC	4 cut(s)	217, 643, 1196, 1277
AfiI	CCNNNNNNNGG	6 cut(s)	60, 418, 668, 923, 1078, 1079
AflII	CTTAAG	1 cut(s)	721
AgsI	TTSAA	6 cut(s)	23, 137, 395, 679, 1127, 1319
AjiI	CACGTC	1 cut(s)	920
AjnI	CCWGG	1 cut(s)	211
AloI	GAACNNNNNNTCC	2 cut(s)	165, 197
AluBI	AGCT	9 cut(s)	133, 164, 374, 577, 620, 666, 868, 911, 1235
AluI	AGCT	9 cut(s)	133, 164, 374, 577, 620, 666, 868, 911, 1235
Alw21I	GWGCWC	2 cut(s)	84, 971
Alw26I	GTCTC	5 cut(s)	108, 532, 666, 794, 1337
AlwI	GGATC	7 cut(s)	4, 210, 228, 460, 908, 1030, 1043
AlwNI	CAGNNNCTG	1 cut(s)	944
Aor13HI	TCCGGA	1 cut(s)	236
ApeKI	GCWGC	1 cut(s)	908
AspLEI	GCGC	1 cut(s)	817
AspS9I	GGNCC	2 cut(s)	239, 604
AsuC2I	CCSGG	1 cut(s)	732
AsuHPI	GGTGA	3 cut(s)	778, 847, 1316
AvaII	GGWCC	2 cut(s)	239, 604
AxyI	CCTNAGG	1 cut(s)	752
BamHI	GGATCC	1 cut(s)	1035
BanII	GRGCYC	1 cut(s)	1086
BauI	CACGAG	1 cut(s)	186
Bbv12I	GWGCWC	2 cut(s)	84, 971
BbvI	GCAGC	1 cut(s)	920
BccI	CCATC	2 cut(s)	523, 579
BceAI	ACGGC	2 cut(s)	100, 1176
BcgI	CGANNNNNNTGC	2 cut(s)	589, 623
BciT130I	CCWGG	1 cut(s)	213
BciVI	GTATCC	1 cut(s)	489
BcnI	CCSGG	1 cut(s)	732
BcoDI	GTCTC	5 cut(s)	108, 532, 666, 794, 1337
BfaI	CTAG	1 cut(s)	663
BfmI	CTRYAG	1 cut(s)	120
BfrI	CTTAAG	1 cut(s)	721
BfuI	GTATCC	1 cut(s)	489
BglII	AGATCT	2 cut(s)	124, 252
BisI	GCNGC	2 cut(s)	909, 1095
BlsI	GCNGC	2 cut(s)	910, 1096
Bme1390I	CCNGG	2 cut(s)	213, 732
Bme18I	GGWCC	2 cut(s)	239, 604
BmgBI	CACGTC	1 cut(s)	920
BmgT120I	GGNCC	2 cut(s)	239, 604
BmiI	GGNNCC	3 cut(s)	52, 728, 1037
BmrFI	CCNGG	2 cut(s)	213, 732
BmsI	GCATC	4 cut(s)	96, 334, 483, 1273
BpmI	CTGGAG	1 cut(s)	113
Bpu10I	CCTNAGC	1 cut(s)	864
BpuEI	CTTGAG	1 cut(s)	227
BpuMI	CCSGG	1 cut(s)	732
Bsa29I	ATCGAT	1 cut(s)	90
BsaHI	GRCGYC	1 cut(s)	1118
BsaJI	CCNNGG	2 cut(s)	730, 829
BsaWI	WCCGGW	2 cut(s)	98, 236
BsaXI	ACNNNNNCTCC	2 cut(s)	1132, 1162
Bsc4I	CCNNNNNNNGG	6 cut(s)	60, 418, 668, 923, 1078, 1079
Bse21I	CCTNAGG	1 cut(s)	752
BseAI	TCCGGA	1 cut(s)	236
BseBI	CCWGG	1 cut(s)	213
BseCI	ATCGAT	1 cut(s)	90
BseDI	CCNNGG	2 cut(s)	730, 829
BseLI	CCNNNNNNNGG	6 cut(s)	60, 418, 668, 923, 1078, 1079
BseMII	CTCAG	5 cut(s)	366, 667, 766, 855, 1227
BseRI	GAGGAG	3 cut(s)	438, 488, 1343
BseXI	GCAGC	1 cut(s)	920
BsgI	GTGCAG	1 cut(s)	927
Bsh1236I	CGCG	1 cut(s)	764
BshVI	ATCGAT	1 cut(s)	90
BsiHKAI	GWGCWC	2 cut(s)	84, 971
BsiSI	CCGG	3 cut(s)	99, 237, 732
BslFI	GGGAC	1 cut(s)	899
BslI	CCNNNNNNNGG	6 cut(s)	60, 418, 668, 923, 1078, 1079
BsmAI	GTCTC	5 cut(s)	108, 532, 666, 794, 1337
BsmBI	CGTCTC	1 cut(s)	794
BsmFI	GGGAC	1 cut(s)	899
BsmI	GAATGC	1 cut(s)	602
Bsp1286I	GDGCHC	3 cut(s)	84, 971, 1086
Bsp13I	TCCGGA	1 cut(s)	236
Bsp143I	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
BspACI	CCGC	5 cut(s)	36, 693, 705, 764, 1094
BspCNI	CTCAG	5 cut(s)	367, 666, 765, 856, 1228
BspDI	ATCGAT	1 cut(s)	90
BspEI	TCCGGA	1 cut(s)	236
BspFNI	CGCG	1 cut(s)	764
BspLI	GGNNCC	3 cut(s)	52, 728, 1037
BspMAI	CTGCAG	1 cut(s)	124
BspPI	GGATC	7 cut(s)	4, 210, 228, 460, 908, 1030, 1043
BspTI	CTTAAG	1 cut(s)	721
BsrBI	CCGCTC	1 cut(s)	705
BssECI	CCNNGG	2 cut(s)	730, 829
BssMI	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
BssNI	GRCGYC	1 cut(s)	1118
BssSI	CACGAG	1 cut(s)	186
Bst2BI	CACGAG	1 cut(s)	186
Bst2UI	CCWGG	1 cut(s)	213
Bst4CI	ACNGT	5 cut(s)	110, 243, 538, 859, 1275
Bst6I	CTCTTC	1 cut(s)	1320
BstACI	GRCGYC	1 cut(s)	1118
BstAFI	CTTAAG	1 cut(s)	721
BstC8I	GCNNGC	1 cut(s)	836
BstDEI	CTNAG	5 cut(s)	375, 653, 752, 864, 1236
BstDSI	CCRYGG	1 cut(s)	829
BstEII	GGTNACC	1 cut(s)	38
BstFNI	CGCG	1 cut(s)	764
BstHHI	GCGC	1 cut(s)	817
BstKTI	GATC	9 cut(s)	12, 127, 205, 236, 255, 468, 690, 916, 1038
BstMAI	GTCTC	5 cut(s)	108, 532, 666, 794, 1337
BstMBI	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
BstMWI	GCNNNNNNNGC	1 cut(s)	663
BstNI	CCWGG	1 cut(s)	213
BstPI	GGTNACC	1 cut(s)	38
BstSCI	CCNGG	2 cut(s)	211, 730
BstSFI	CTRYAG	1 cut(s)	120
BstUI	CGCG	1 cut(s)	764
BstV1I	GCAGC	1 cut(s)	920
BstX2I	RGATCY	7 cut(s)	9, 124, 202, 233, 252, 465, 1035
BstYI	RGATCY	7 cut(s)	9, 124, 202, 233, 252, 465, 1035
Bsu15I	ATCGAT	1 cut(s)	90
Bsu36I	CCTNAGG	1 cut(s)	752
BsuI	GTATCC	1 cut(s)	489
BsuTUI	ATCGAT	1 cut(s)	90
BtgI	CCRYGG	1 cut(s)	829
BtrI	CACGTC	1 cut(s)	920
BtsI	GCAGTG	1 cut(s)	117
BtsIMutI	CAGTG	3 cut(s)	115, 117, 321
Cac8I	GCNNGC	1 cut(s)	836
CaiI	CAGNNNCTG	1 cut(s)	944
CfoI	GCGC	1 cut(s)	817
Cfr13I	GGNCC	2 cut(s)	239, 604
ClaI	ATCGAT	1 cut(s)	90
CpoI	CGGWCCG	1 cut(s)	239
Csp6I	GTAC	4 cut(s)	216, 642, 1195, 1276
CspCI	CAANNNNNGTGG	2 cut(s)	863, 898
CspI	CGGWCCG	1 cut(s)	239
CviAII	CATG	3 cut(s)	601, 802, 898
CviQI	GTAC	4 cut(s)	216, 642, 1195, 1276
DdeI	CTNAG	5 cut(s)	375, 653, 752, 864, 1236
DpnI	GATC	9 cut(s)	11, 126, 204, 235, 254, 467, 689, 915, 1037
DpnII	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
DrdI	GACNNNNNNGTC	1 cut(s)	246
DseDI	GACNNNNNNGTC	1 cut(s)	246
Eam1104I	CTCTTC	1 cut(s)	1320
EarI	CTCTTC	1 cut(s)	1320
Eco24I	GRGCYC	1 cut(s)	1086
Eco32I	GATATC	3 cut(s)	459, 631, 1069
Eco47I	GGWCC	2 cut(s)	239, 604
Eco57I	CTGAAG	2 cut(s)	150, 345
Eco81I	CCTNAGG	1 cut(s)	752
Eco91I	GGTNACC	1 cut(s)	38
EcoO65I	GGTNACC	1 cut(s)	38
EcoRII	CCWGG	1 cut(s)	211
EcoRV	GATATC	3 cut(s)	459, 631, 1069
EcoT22I	ATGCAT	1 cut(s)	602
EcoT38I	GRGCYC	1 cut(s)	1086
Esp3I	CGTCTC	1 cut(s)	794
FaeI	CATG	3 cut(s)	604, 805, 901
FaqI	GGGAC	1 cut(s)	899
FatI	CATG	3 cut(s)	600, 801, 897
FblI	GTMKAC	1 cut(s)	981
Fnu4HI	GCNGC	2 cut(s)	909, 1095
FriOI	GRGCYC	1 cut(s)	1086
Fsp4HI	GCNGC	2 cut(s)	909, 1095
FspBI	CTAG	1 cut(s)	663
GlaI	GCGC	1 cut(s)	816
GluI	GCNGC	2 cut(s)	909, 1095
GsuI	CTGGAG	1 cut(s)	113
HapII	CCGG	3 cut(s)	99, 237, 732
HhaI	GCGC	1 cut(s)	817
Hin1I	GRCGYC	1 cut(s)	1118
Hin1II	CATG	3 cut(s)	604, 805, 901
Hin6I	GCGC	1 cut(s)	815
HinP1I	GCGC	1 cut(s)	815
HincII	GTYRAC	1 cut(s)	982
HindII	GTYRAC	1 cut(s)	982
HindIII	AAGCTT	1 cut(s)	162
HinfI	GANTC	4 cut(s)	92, 871, 887, 1123
HpaII	CCGG	3 cut(s)	99, 237, 732
HphI	GGTGA	3 cut(s)	778, 847, 1316
Hpy166II	GTNNAC	5 cut(s)	769, 982, 1158, 1197, 1227
Hpy188I	TCNGA	6 cut(s)	233, 252, 470, 532, 748, 956
Hpy8I	GTNNAC	5 cut(s)	769, 982, 1158, 1197, 1227
Hpy99I	CGWCG	5 cut(s)	80, 986, 1102, 1120, 1123
HpyAV	CCTTC	5 cut(s)	23, 320, 401, 1199, 1295
HpyCH4III	ACNGT	5 cut(s)	110, 243, 538, 859, 1275
HpyCH4IV	ACGT	8 cut(s)	103, 139, 180, 919, 928, 1047, 1100, 1118
HpyCH4V	TGCA	5 cut(s)	122, 557, 600, 908, 1189
HpyF10VI	GCNNNNNNNGC	1 cut(s)	663
HpyF3I	CTNAG	5 cut(s)	375, 653, 752, 864, 1236
HpySE526I	ACGT	8 cut(s)	103, 139, 180, 919, 928, 1047, 1100, 1118
Hsp92I	GRCGYC	1 cut(s)	1118
Hsp92II	CATG	3 cut(s)	604, 805, 901
HspAI	GCGC	1 cut(s)	815
Kpn2I	TCCGGA	1 cut(s)	236
Kzo9I	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
LmnI	GCTCC	1 cut(s)	722
Lsp1109I	GCAGC	1 cut(s)	920
LweI	GCATC	4 cut(s)	96, 334, 483, 1273
MaeI	CTAG	1 cut(s)	663
MaeII	ACGT	8 cut(s)	103, 139, 180, 919, 928, 1047, 1100, 1118
MaeIII	GTNAC	6 cut(s)	38, 104, 709, 920, 929, 1109
MalI	GATC	9 cut(s)	11, 126, 204, 235, 254, 467, 689, 915, 1037
MbiI	CCGCTC	1 cut(s)	705
MboI	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
MboII	GAAGA	7 cut(s)	186, 247, 475, 790, 1331, 1334, 1337
MflI	RGATCY	7 cut(s)	9, 124, 202, 233, 252, 465, 1035
MhlI	GDGCHC	3 cut(s)	84, 971, 1086
MluCI	AATT	1 cut(s)	301
MlyI	GAGTC	2 cut(s)	865, 881
Mph1103I	ATGCAT	1 cut(s)	602
MroI	TCCGGA	1 cut(s)	236
MseI	TTAA	1 cut(s)	722
MslI	CAYNNNNRTG	1 cut(s)	1163
MspA1I	CMGCKG	1 cut(s)	911
MspCI	CTTAAG	1 cut(s)	721
MspI	CCGG	3 cut(s)	99, 237, 732
MspR9I	CCNGG	2 cut(s)	213, 732
Mva1269I	GAATGC	1 cut(s)	602
MvaI	CCWGG	1 cut(s)	213
MvnI	CGCG	1 cut(s)	764
MwoI	GCNNNNNNNGC	1 cut(s)	663
NciI	CCSGG	1 cut(s)	732
NdeII	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
NlaIII	CATG	3 cut(s)	604, 805, 901
NlaIV	GGNNCC	3 cut(s)	52, 728, 1037
NmuCI	GTSAC	2 cut(s)	709, 920
NsiI	ATGCAT	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	1 cut(s)	925
PctI	GAATGC	1 cut(s)	602
PfeI	GAWTC	2 cut(s)	92, 1123
PflFI	GACNNNGTC	1 cut(s)	536
PflMI	CCANNNNNTGG	2 cut(s)	60, 418
PkrI	GCNGC	2 cut(s)	910, 1096
PleI	GAGTC	2 cut(s)	865, 881
PpsI	GAGTC	2 cut(s)	865, 881
Psp6I	CCWGG	1 cut(s)	211
PspEI	GGTNACC	1 cut(s)	38
PspGI	CCWGG	1 cut(s)	211
PspN4I	GGNNCC	3 cut(s)	52, 728, 1037
PspPI	GGNCC	2 cut(s)	239, 604
PsrI	GAACNNNNNNTAC	2 cut(s)	1004, 1036
PstI	CTGCAG	1 cut(s)	124
PstNI	CAGNNNCTG	1 cut(s)	944
PsuI	RGATCY	7 cut(s)	9, 124, 202, 233, 252, 465, 1035
PsyI	GACNNNGTC	1 cut(s)	536
PvuII	CAGCTG	1 cut(s)	911
RsaI	GTAC	4 cut(s)	217, 643, 1196, 1277
RsaNI	GTAC	4 cut(s)	216, 642, 1195, 1276
RseI	CAYNNNNRTG	1 cut(s)	1163
Rsr2I	CGGWCCG	1 cut(s)	239
RsrII	CGGWCCG	1 cut(s)	239
SalI	GTCGAC	1 cut(s)	980
SaqAI	TTAA	1 cut(s)	722
SatI	GCNGC	2 cut(s)	909, 1095
Sau3AI	GATC	9 cut(s)	9, 124, 202, 233, 252, 465, 687, 913, 1035
Sau96I	GGNCC	2 cut(s)	239, 604
SchI	GAGTC	2 cut(s)	865, 881
ScrFI	CCNGG	2 cut(s)	213, 732
SduI	GDGCHC	3 cut(s)	84, 971, 1086
SfaNI	GCATC	4 cut(s)	96, 334, 483, 1273
SfcI	CTRYAG	1 cut(s)	120
SinI	GGWCC	2 cut(s)	239, 604
SmiMI	CAYNNNNRTG	1 cut(s)	1163
SmlI	CTYRAG	2 cut(s)	206, 721
SmoI	CTYRAG	2 cut(s)	206, 721
Sse9I	AATT	1 cut(s)	301
SsiI	CCGC	5 cut(s)	36, 693, 705, 764, 1094
SspMI	CTAG	1 cut(s)	663
StyD4I	CCNGG	2 cut(s)	211, 730
TaaI	ACNGT	5 cut(s)	110, 243, 538, 859, 1275
TaiI	ACGT	8 cut(s)	106, 142, 183, 922, 931, 1050, 1103, 1121
TaqI	TCGA	6 cut(s)	90, 461, 609, 981, 1115, 1121
TasI	AATT	1 cut(s)	301
TauI	GCSGC	1 cut(s)	1097
TfiI	GAWTC	2 cut(s)	92, 1123
Tru1I	TTAA	1 cut(s)	722
Tru9I	TTAA	1 cut(s)	722
TscAI	CASTG	3 cut(s)	115, 124, 328
TseFI	GTSAC	2 cut(s)	709, 920
TseI	GCWGC	1 cut(s)	908
Tsp45I	GTSAC	2 cut(s)	709, 920
TspDTI	ATGAA	5 cut(s)	174, 609, 630, 790, 1262
TspGWI	ACGGA	4 cut(s)	248, 629, 939, 1244
TspRI	CASTG	3 cut(s)	115, 124, 328
Tth111I	GACNNNGTC	1 cut(s)	536
Van91I	CCANNNNNTGG	2 cut(s)	60, 418
Vha464I	CTTAAG	1 cut(s)	721
VpaK11BI	GGWCC	2 cut(s)	239, 604
XmiI	GTMKAC	1 cut(s)	981
XspI	CTAG	1 cut(s)	663
ZraI	GACGTC	1 cut(s)	1119
Zsp2I	ATGCAT	1 cut(s)	602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.