Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G20010 AT1G75780 |
| fragaria_vesca | FvH4_2g38400 FvH4_2g38400 |
| malus_domestica | MD08G1012800.v1.1 MD15G1011800.v1.1 |
| prunus_persica | Prupe.1G364800_v2.0.a1 |
| pyrus_communis | pycom08g01090 pycom15g01040 |
| rosa_chinensis | RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301 |
| rosa_laevigata | RLG00000011125 |
| rosa_multiflora | Rmu_ssc0000460.1_g000016 |
| rosa_rugosa | Rorug06G0318000 |
| rosa_samantha | Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800 |
| rosa_wichuraiana | Rw6G037410 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 275 |
| Acc65I | GGTACC | 2 cut(s) | 105, 218 |
| AccB1I | GGYRCC | 4 cut(s) | 105, 218, 293, 332 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 60, 671 |
| AccI | GTMKAC | 1 cut(s) | 1100 |
| AccII | CGCG | 1 cut(s) | 192 |
| AccIII | TCCGGA | 1 cut(s) | 756 |
| AciI | CCGC | 3 cut(s) | 36, 174, 262 |
| AclWI | GGATC | 4 cut(s) | 152, 213, 231, 1120 |
| AcoI | YGGCCR | 3 cut(s) | 175, 250, 283 |
| AcsI | RAATTY | 1 cut(s) | 1015 |
| AcuI | CTGAAG | 2 cut(s) | 625, 1353 |
| AcyI | GRCGYC | 2 cut(s) | 117, 1097 |
| AdeI | CACNNNGTG | 1 cut(s) | 1051 |
| AfaI | GTAC | 7 cut(s) | 107, 182, 220, 848, 1199, 1271, 1313 |
| AgsI | TTSAA | 4 cut(s) | 134, 212, 398, 1090 |
| AjiI | CACGTC | 1 cut(s) | 791 |
| AjnI | CCWGG | 3 cut(s) | 733, 1079, 1128 |
| AleI | CACNNNNGTG | 1 cut(s) | 542 |
| AluBI | AGCT | 6 cut(s) | 655, 669, 742, 763, 871, 1238 |
| AluI | AGCT | 6 cut(s) | 655, 669, 742, 763, 871, 1238 |
| Alw21I | GWGCWC | 3 cut(s) | 84, 576, 974 |
| Alw26I | GTCTC | 3 cut(s) | 108, 535, 669 |
| Alw44I | GTGCAC | 1 cut(s) | 572 |
| AlwI | GGATC | 4 cut(s) | 152, 213, 231, 1120 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 539, 1238 |
| Ama87I | CYCGRG | 1 cut(s) | 137 |
| Aor13HI | TCCGGA | 1 cut(s) | 756 |
| AoxI | GGCC | 7 cut(s) | 28, 175, 250, 283, 309, 375, 1182 |
| ApaLI | GTGCAC | 1 cut(s) | 572 |
| ApeKI | GCWGC | 3 cut(s) | 720, 739, 911 |
| ApoI | RAATTY | 1 cut(s) | 1015 |
| Asp718I | GGTACC | 2 cut(s) | 105, 218 |
| AspLEI | GCGC | 2 cut(s) | 194, 1179 |
| AspS9I | GGNCC | 4 cut(s) | 242, 309, 645, 837 |
| AsuC2I | CCSGG | 1 cut(s) | 216 |
| AsuHPI | GGTGA | 3 cut(s) | 513, 724, 1319 |
| AvaI | CYCGRG | 1 cut(s) | 137 |
| AvaII | GGWCC | 3 cut(s) | 242, 645, 837 |
| AxyI | CCTNAGG | 1 cut(s) | 725 |
| BaeGI | GKGCMC | 1 cut(s) | 576 |
| BanI | GGYRCC | 4 cut(s) | 105, 218, 293, 332 |
| BauI | CACGAG | 1 cut(s) | 831 |
| BbsI | GAAGAC | 2 cut(s) | 271, 785 |
| Bbv12I | GWGCWC | 3 cut(s) | 84, 576, 974 |
| BbvCI | CCTCAGC | 1 cut(s) | 946 |
| BbvI | GCAGC | 3 cut(s) | 707, 751, 898 |
| BccI | CCATC | 3 cut(s) | 1207, 1216, 1298 |
| BceAI | ACGGC | 2 cut(s) | 100, 179 |
| BciT130I | CCWGG | 3 cut(s) | 735, 1081, 1130 |
| BclI | TGATCA | 1 cut(s) | 996 |
| BcnI | CCSGG | 1 cut(s) | 216 |
| BcoDI | GTCTC | 3 cut(s) | 108, 535, 669 |
| BglII | AGATCT | 2 cut(s) | 9, 255 |
| BisI | GCNGC | 4 cut(s) | 175, 721, 740, 912 |
| BlpI | GCTNAGC | 1 cut(s) | 656 |
| BlsI | GCNGC | 4 cut(s) | 176, 722, 741, 913 |
| Bme1390I | CCNGG | 4 cut(s) | 216, 735, 1081, 1130 |
| Bme18I | GGWCC | 3 cut(s) | 242, 645, 837 |
| BmeT110I | CYCGRG | 1 cut(s) | 137 |
| BmgBI | CACGTC | 1 cut(s) | 791 |
| BmgT120I | GGNCC | 4 cut(s) | 242, 309, 645, 837 |
| BmrFI | CCNGG | 4 cut(s) | 216, 735, 1081, 1130 |
| BmrI | ACTGGG | 3 cut(s) | 316, 444, 1212 |
| BmsI | GCATC | 4 cut(s) | 240, 486, 880, 1276 |
| BmuI | ACTGGG | 3 cut(s) | 316, 444, 1212 |
| BoxI | GACNNNNGTC | 1 cut(s) | 921 |
| BpiI | GAAGAC | 2 cut(s) | 271, 785 |
| Bpu10I | CCTNAGC | 1 cut(s) | 946 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 656 |
| BpuMI | CCSGG | 1 cut(s) | 216 |
| Bsa29I | ATCGAT | 2 cut(s) | 345, 1124 |
| BsaHI | GRCGYC | 2 cut(s) | 117, 1097 |
| BsaI | GGTCTC | 2 cut(s) | 535, 669 |
| BsaJI | CCNNGG | 8 cut(s) | 22, 109, 222, 733, 734, 975, 1079, 1080 |
| BsaWI | WCCGGW | 4 cut(s) | 98, 239, 290, 756 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 107, 137 |
| Bse118I | RCCGGY | 2 cut(s) | 177, 296 |
| Bse1I | ACTGG | 5 cut(s) | 286, 311, 439, 577, 1207 |
| Bse21I | CCTNAGG | 1 cut(s) | 725 |
| Bse3DI | GCAATG | 1 cut(s) | 957 |
| BseAI | TCCGGA | 1 cut(s) | 756 |
| BseBI | CCWGG | 3 cut(s) | 735, 1081, 1130 |
| BseCI | ATCGAT | 2 cut(s) | 345, 1124 |
| BseDI | CCNNGG | 8 cut(s) | 22, 109, 222, 733, 734, 975, 1079, 1080 |
| BseGI | GGATG | 6 cut(s) | 231, 498, 895, 991, 1218, 1306 |
| BseMI | GCAATG | 1 cut(s) | 957 |
| BseMII | CTCAG | 5 cut(s) | 546, 670, 890, 960, 1230 |
| BseNI | ACTGG | 5 cut(s) | 286, 311, 439, 577, 1207 |
| BseRI | GAGGAG | 3 cut(s) | 441, 1331, 1334 |
| BseSI | GKGCMC | 1 cut(s) | 576 |
| BseX3I | CGGCCG | 1 cut(s) | 175 |
| BseXI | GCAGC | 3 cut(s) | 707, 751, 898 |
| BsgI | GTGCAG | 1 cut(s) | 1025 |
| Bsh1236I | CGCG | 1 cut(s) | 192 |
| Bsh1285I | CGRYCG | 1 cut(s) | 178 |
| BshFI | GGCC | 7 cut(s) | 30, 177, 252, 285, 311, 377, 1184 |
| BshNI | GGYRCC | 4 cut(s) | 105, 218, 293, 332 |
| BshVI | ATCGAT | 2 cut(s) | 345, 1124 |
| BsiEI | CGRYCG | 1 cut(s) | 178 |
| BsiHKAI | GWGCWC | 3 cut(s) | 84, 576, 974 |
| BsiHKCI | CYCGRG | 1 cut(s) | 137 |
| BsiSI | CCGG | 9 cut(s) | 99, 171, 178, 216, 240, 291, 297, 757, 1174 |
| BslFI | GGGAC | 2 cut(s) | 170, 823 |
| BsmAI | GTCTC | 3 cut(s) | 108, 535, 669 |
| BsmBI | CGTCTC | 1 cut(s) | 108 |
| BsmFI | GGGAC | 2 cut(s) | 170, 823 |
| BsmI | GAATGC | 2 cut(s) | 595, 605 |
| BsnI | GGCC | 7 cut(s) | 30, 177, 252, 285, 311, 377, 1184 |
| Bso31I | GGTCTC | 2 cut(s) | 535, 669 |
| BsoBI | CYCGRG | 1 cut(s) | 137 |
| Bsp1286I | GDGCHC | 3 cut(s) | 84, 576, 974 |
| Bsp13I | TCCGGA | 1 cut(s) | 756 |
| Bsp143I | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 656 |
| Bsp19I | CCATGG | 1 cut(s) | 222 |
| BspACI | CCGC | 3 cut(s) | 36, 174, 262 |
| BspANI | GGCC | 7 cut(s) | 30, 177, 252, 285, 311, 377, 1184 |
| BspCNI | CTCAG | 5 cut(s) | 545, 669, 889, 959, 1231 |
| BspDI | ATCGAT | 2 cut(s) | 345, 1124 |
| BspEI | TCCGGA | 1 cut(s) | 756 |
| BspFNI | CGCG | 1 cut(s) | 192 |
| BspPI | GGATC | 4 cut(s) | 152, 213, 231, 1120 |
| BspT107I | GGYRCC | 4 cut(s) | 105, 218, 293, 332 |
| BspTNI | GGTCTC | 2 cut(s) | 535, 669 |
| BsrDI | GCAATG | 1 cut(s) | 957 |
| BsrFI | RCCGGY | 2 cut(s) | 177, 296 |
| BsrI | ACTGG | 5 cut(s) | 286, 311, 439, 577, 1207 |
| BssAI | RCCGGY | 2 cut(s) | 177, 296 |
| BssECI | CCNNGG | 8 cut(s) | 22, 109, 222, 733, 734, 975, 1079, 1080 |
| BssMI | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| BssNI | GRCGYC | 2 cut(s) | 117, 1097 |
| BssSI | CACGAG | 1 cut(s) | 831 |
| BssT1I | CCWWGG | 3 cut(s) | 22, 222, 975 |
| Bst2BI | CACGAG | 1 cut(s) | 831 |
| Bst2UI | CCWGG | 3 cut(s) | 735, 1081, 1130 |
| Bst4CI | ACNGT | 6 cut(s) | 110, 541, 846, 862, 1294, 1328 |
| Bst6I | CTCTTC | 1 cut(s) | 1329 |
| BstACI | GRCGYC | 2 cut(s) | 117, 1097 |
| BstC8I | GCNNGC | 3 cut(s) | 298, 765, 1154 |
| BstDEI | CTNAG | 6 cut(s) | 532, 656, 725, 876, 946, 1239 |
| BstDSI | CCRYGG | 2 cut(s) | 109, 222 |
| BstEII | GGTNACC | 1 cut(s) | 38 |
| BstF5I | GGATG | 6 cut(s) | 231, 498, 895, 991, 1218, 1306 |
| BstFNI | CGCG | 1 cut(s) | 192 |
| BstHHI | GCGC | 2 cut(s) | 194, 1179 |
| BstKTI | GATC | 9 cut(s) | 12, 130, 147, 208, 239, 258, 345, 999, 1128 |
| BstMAI | GTCTC | 3 cut(s) | 108, 535, 669 |
| BstMBI | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| BstMCI | CGRYCG | 1 cut(s) | 178 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 666, 877 |
| BstNI | CCWGG | 3 cut(s) | 735, 1081, 1130 |
| BstNSI | RCATGY | 1 cut(s) | 720 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 921 |
| BstPI | GGTNACC | 1 cut(s) | 38 |
| BstSCI | CCNGG | 4 cut(s) | 214, 733, 1079, 1128 |
| BstSLI | GKGCMC | 1 cut(s) | 576 |
| BstUI | CGCG | 1 cut(s) | 192 |
| BstV1I | GCAGC | 3 cut(s) | 707, 751, 898 |
| BstV2I | GAAGAC | 2 cut(s) | 271, 785 |
| BstX2I | RGATCY | 3 cut(s) | 9, 205, 255 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 422, 982 |
| BstYI | RGATCY | 3 cut(s) | 9, 205, 255 |
| BstZI | CGGCCG | 1 cut(s) | 175 |
| Bsu15I | ATCGAT | 2 cut(s) | 345, 1124 |
| Bsu36I | CCTNAGG | 1 cut(s) | 725 |
| BsuRI | GGCC | 7 cut(s) | 30, 177, 252, 285, 311, 377, 1184 |
| BsuTUI | ATCGAT | 2 cut(s) | 345, 1124 |
| BtgI | CCRYGG | 2 cut(s) | 109, 222 |
| BtrI | CACGTC | 1 cut(s) | 791 |
| BtsCI | GGATG | 6 cut(s) | 231, 498, 895, 991, 1218, 1306 |
| BtsIMutI | CAGTG | 5 cut(s) | 432, 537, 576, 858, 1200 |
| Cac8I | GCNNGC | 3 cut(s) | 298, 765, 1154 |
| CaiI | CAGNNNCTG | 2 cut(s) | 539, 1238 |
| CfoI | GCGC | 2 cut(s) | 194, 1179 |
| Cfr10I | RCCGGY | 2 cut(s) | 177, 296 |
| Cfr13I | GGNCC | 4 cut(s) | 242, 309, 645, 837 |
| ClaI | ATCGAT | 2 cut(s) | 345, 1124 |
| CpoI | CGGWCCG | 1 cut(s) | 242 |
| CseI | GACGC | 2 cut(s) | 125, 1086 |
| Csp6I | GTAC | 7 cut(s) | 106, 181, 219, 847, 1198, 1270, 1312 |
| CspI | CGGWCCG | 1 cut(s) | 242 |
| CviQI | GTAC | 7 cut(s) | 106, 181, 219, 847, 1198, 1270, 1312 |
| DdeI | CTNAG | 6 cut(s) | 532, 656, 725, 876, 946, 1239 |
| DpnI | GATC | 9 cut(s) | 11, 129, 146, 207, 238, 257, 344, 998, 1127 |
| DpnII | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| DraIII | CACNNNGTG | 1 cut(s) | 1051 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 275 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 275 |
| EaeI | YGGCCR | 3 cut(s) | 175, 250, 283 |
| EagI | CGGCCG | 1 cut(s) | 175 |
| Eam1104I | CTCTTC | 1 cut(s) | 1329 |
| EarI | CTCTTC | 1 cut(s) | 1329 |
| EciI | GGCGGA | 1 cut(s) | 251 |
| EclXI | CGGCCG | 1 cut(s) | 175 |
| Eco130I | CCWWGG | 3 cut(s) | 22, 222, 975 |
| Eco147I | AGGCCT | 1 cut(s) | 1184 |
| Eco31I | GGTCTC | 2 cut(s) | 535, 669 |
| Eco32I | GATATC | 2 cut(s) | 462, 693 |
| Eco47I | GGWCC | 3 cut(s) | 242, 645, 837 |
| Eco52I | CGGCCG | 1 cut(s) | 175 |
| Eco57I | CTGAAG | 2 cut(s) | 625, 1353 |
| Eco81I | CCTNAGG | 1 cut(s) | 725 |
| Eco88I | CYCGRG | 1 cut(s) | 137 |
| Eco91I | GGTNACC | 1 cut(s) | 38 |
| EcoO109I | RGGNCCY | 2 cut(s) | 645, 837 |
| EcoO65I | GGTNACC | 1 cut(s) | 38 |
| EcoRI | GAATTC | 1 cut(s) | 1015 |
| EcoRII | CCWGG | 3 cut(s) | 733, 1079, 1128 |
| EcoRV | GATATC | 2 cut(s) | 462, 693 |
| EcoT14I | CCWWGG | 3 cut(s) | 22, 222, 975 |
| EcoT22I | ATGCAT | 1 cut(s) | 605 |
| ErhI | CCWWGG | 3 cut(s) | 22, 222, 975 |
| Esp3I | CGTCTC | 1 cut(s) | 108 |
| FaqI | GGGAC | 2 cut(s) | 170, 823 |
| FbaI | TGATCA | 1 cut(s) | 996 |
| FblI | GTMKAC | 1 cut(s) | 1100 |
| Fnu4HI | GCNGC | 4 cut(s) | 175, 721, 740, 912 |
| FokI | GGATG | 6 cut(s) | 218, 505, 902, 998, 1225, 1313 |
| Fsp4HI | GCNGC | 4 cut(s) | 175, 721, 740, 912 |
| GlaI | GCGC | 2 cut(s) | 193, 1178 |
| GluI | GCNGC | 4 cut(s) | 175, 721, 740, 912 |
| HaeIII | GGCC | 7 cut(s) | 30, 177, 252, 285, 311, 377, 1184 |
| HapII | CCGG | 9 cut(s) | 99, 171, 178, 216, 240, 291, 297, 757, 1174 |
| HgaI | GACGC | 2 cut(s) | 125, 1086 |
| HhaI | GCGC | 2 cut(s) | 194, 1179 |
| Hin1I | GRCGYC | 2 cut(s) | 117, 1097 |
| Hin6I | GCGC | 2 cut(s) | 192, 1177 |
| HinP1I | GCGC | 2 cut(s) | 192, 1177 |
| HincII | GTYRAC | 2 cut(s) | 91, 1101 |
| HindII | GTYRAC | 2 cut(s) | 91, 1101 |
| HindIII | AAGCTT | 1 cut(s) | 667 |
| HinfI | GANTC | 4 cut(s) | 347, 772, 778, 1039 |
| HpaII | CCGG | 9 cut(s) | 99, 171, 178, 216, 240, 291, 297, 757, 1174 |
| HphI | GGTGA | 3 cut(s) | 513, 724, 1319 |
| Hpy166II | GTNNAC | 6 cut(s) | 91, 574, 1101, 1161, 1200, 1230 |
| Hpy188I | TCNGA | 6 cut(s) | 127, 236, 459, 535, 751, 777 |
| Hpy188III | TCNNGA | 7 cut(s) | 139, 209, 356, 611, 643, 757, 1141 |
| Hpy8I | GTNNAC | 6 cut(s) | 91, 574, 1101, 1161, 1200, 1230 |
| Hpy99I | CGWCG | 3 cut(s) | 80, 1102, 1298 |
| HpyAV | CCTTC | 6 cut(s) | 96, 323, 404, 464, 528, 1202 |
| HpyCH4III | ACNGT | 6 cut(s) | 110, 541, 846, 862, 1294, 1328 |
| HpyCH4IV | ACGT | 4 cut(s) | 150, 183, 790, 931 |
| HpyCH4V | TGCA | 6 cut(s) | 16, 574, 603, 698, 1006, 1192 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 666, 877 |
| HpyF3I | CTNAG | 6 cut(s) | 532, 656, 725, 876, 946, 1239 |
| HpySE526I | ACGT | 4 cut(s) | 150, 183, 790, 931 |
| Hsp92I | GRCGYC | 2 cut(s) | 117, 1097 |
| HspAI | GCGC | 2 cut(s) | 192, 1177 |
| KflI | GGGWCCC | 1 cut(s) | 837 |
| Kpn2I | TCCGGA | 1 cut(s) | 756 |
| KpnI | GGTACC | 2 cut(s) | 109, 222 |
| KroI | GCCGGC | 1 cut(s) | 296 |
| KroNI | GCCGGC | 1 cut(s) | 298 |
| Ksp22I | TGATCA | 1 cut(s) | 996 |
| Kzo9I | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| LmnI | GCTCC | 1 cut(s) | 56 |
| Lsp1109I | GCAGC | 3 cut(s) | 707, 751, 898 |
| LweI | GCATC | 4 cut(s) | 240, 486, 880, 1276 |
| MaeII | ACGT | 4 cut(s) | 150, 183, 790, 931 |
| MaeIII | GTNAC | 6 cut(s) | 38, 386, 712, 923, 932, 1244 |
| MalI | GATC | 9 cut(s) | 11, 129, 146, 207, 238, 257, 344, 998, 1127 |
| MboI | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| MboII | GAAGA | 6 cut(s) | 122, 250, 271, 785, 793, 1346 |
| MflI | RGATCY | 3 cut(s) | 9, 205, 255 |
| MhlI | GDGCHC | 3 cut(s) | 84, 576, 974 |
| MluCI | AATT | 2 cut(s) | 382, 1015 |
| MmeI | TCCRAC | 1 cut(s) | 774 |
| Mph1103I | ATGCAT | 1 cut(s) | 605 |
| MroI | TCCGGA | 1 cut(s) | 756 |
| MroNI | GCCGGC | 1 cut(s) | 296 |
| MslI | CAYNNNNRTG | 2 cut(s) | 542, 1166 |
| MspA1I | CMGCKG | 1 cut(s) | 742 |
| MspI | CCGG | 9 cut(s) | 99, 171, 178, 216, 240, 291, 297, 757, 1174 |
| MspR9I | CCNGG | 4 cut(s) | 216, 735, 1081, 1130 |
| Mva1269I | GAATGC | 2 cut(s) | 595, 605 |
| MvaI | CCWGG | 3 cut(s) | 735, 1081, 1130 |
| MvnI | CGCG | 1 cut(s) | 192 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 666, 877 |
| NaeI | GCCGGC | 1 cut(s) | 298 |
| NciI | CCSGG | 1 cut(s) | 216 |
| NcoI | CCATGG | 1 cut(s) | 222 |
| NdeII | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| NgoMIV | GCCGGC | 1 cut(s) | 296 |
| NmeAIII | GCCGAG | 3 cut(s) | 361, 403, 1291 |
| NmuCI | GTSAC | 3 cut(s) | 386, 712, 923 |
| NsiI | ATGCAT | 1 cut(s) | 605 |
| NspI | RCATGY | 1 cut(s) | 720 |
| OliI | CACNNNNGTG | 1 cut(s) | 542 |
| PaeR7I | CTCGAG | 1 cut(s) | 137 |
| PasI | CCCWGGG | 2 cut(s) | 734, 1080 |
| PceI | AGGCCT | 1 cut(s) | 1184 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 84, 123, 928 |
| PctI | GAATGC | 2 cut(s) | 595, 605 |
| PdiI | GCCGGC | 1 cut(s) | 298 |
| PfeI | GAWTC | 4 cut(s) | 347, 772, 778, 1039 |
| PflFI | GACNNNGTC | 1 cut(s) | 539 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 60, 671 |
| PfoI | TCCNGGA | 1 cut(s) | 1128 |
| PkrI | GCNGC | 4 cut(s) | 176, 722, 741, 913 |
| PpuMI | RGGWCCY | 2 cut(s) | 645, 837 |
| PshAI | GACNNNNGTC | 1 cut(s) | 921 |
| Psp5II | RGGWCCY | 2 cut(s) | 645, 837 |
| Psp6I | CCWGG | 3 cut(s) | 733, 1079, 1128 |
| PspEI | GGTNACC | 1 cut(s) | 38 |
| PspGI | CCWGG | 3 cut(s) | 733, 1079, 1128 |
| PspPI | GGNCC | 4 cut(s) | 242, 309, 645, 837 |
| PspPPI | RGGWCCY | 2 cut(s) | 645, 837 |
| PstNI | CAGNNNCTG | 2 cut(s) | 539, 1238 |
| PsuI | RGATCY | 3 cut(s) | 9, 205, 255 |
| PsyI | GACNNNGTC | 1 cut(s) | 539 |
| PvuII | CAGCTG | 1 cut(s) | 742 |
| RsaI | GTAC | 7 cut(s) | 107, 182, 220, 848, 1199, 1271, 1313 |
| RsaNI | GTAC | 7 cut(s) | 106, 181, 219, 847, 1198, 1270, 1312 |
| RseI | CAYNNNNRTG | 2 cut(s) | 542, 1166 |
| Rsr2I | CGGWCCG | 1 cut(s) | 242 |
| RsrII | CGGWCCG | 1 cut(s) | 242 |
| SalI | GTCGAC | 1 cut(s) | 1099 |
| SatI | GCNGC | 4 cut(s) | 175, 721, 740, 912 |
| Sau3AI | GATC | 9 cut(s) | 9, 127, 144, 205, 236, 255, 342, 996, 1125 |
| Sau96I | GGNCC | 4 cut(s) | 242, 309, 645, 837 |
| ScrFI | CCNGG | 4 cut(s) | 216, 735, 1081, 1130 |
| SduI | GDGCHC | 3 cut(s) | 84, 576, 974 |
| SfaNI | GCATC | 4 cut(s) | 240, 486, 880, 1276 |
| Sfr274I | CTCGAG | 1 cut(s) | 137 |
| SinI | GGWCC | 3 cut(s) | 242, 645, 837 |
| SlaI | CTCGAG | 1 cut(s) | 137 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 542, 1166 |
| SmlI | CTYRAG | 1 cut(s) | 137 |
| SmoI | CTYRAG | 1 cut(s) | 137 |
| Sse9I | AATT | 2 cut(s) | 382, 1015 |
| SseBI | AGGCCT | 1 cut(s) | 1184 |
| SsiI | CCGC | 3 cut(s) | 36, 174, 262 |
| StuI | AGGCCT | 1 cut(s) | 1184 |
| StyD4I | CCNGG | 4 cut(s) | 214, 733, 1079, 1128 |
| StyI | CCWWGG | 3 cut(s) | 22, 222, 975 |
| TaaI | ACNGT | 6 cut(s) | 110, 541, 846, 862, 1294, 1328 |
| TaiI | ACGT | 4 cut(s) | 153, 186, 793, 934 |
| TaqI | TCGA | 6 cut(s) | 138, 345, 357, 1100, 1124, 1296 |
| TasI | AATT | 2 cut(s) | 382, 1015 |
| TatI | WGTACW | 1 cut(s) | 846 |
| TauI | GCSGC | 1 cut(s) | 177 |
| TfiI | GAWTC | 4 cut(s) | 347, 772, 778, 1039 |
| TscAI | CASTG | 5 cut(s) | 439, 544, 576, 865, 1207 |
| TseFI | GTSAC | 3 cut(s) | 386, 712, 923 |
| TseI | GCWGC | 3 cut(s) | 720, 739, 911 |
| Tsp45I | GTSAC | 3 cut(s) | 386, 712, 923 |
| TspDTI | ATGAA | 5 cut(s) | 612, 633, 793, 1002, 1265 |
| TspGWI | ACGGA | 3 cut(s) | 234, 340, 942 |
| TspRI | CASTG | 5 cut(s) | 439, 544, 576, 865, 1207 |
| Tth111I | GACNNNGTC | 1 cut(s) | 539 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 60, 671 |
| VneI | GTGCAC | 1 cut(s) | 572 |
| VpaK11BI | GGWCC | 3 cut(s) | 242, 645, 837 |
| XapI | RAATTY | 1 cut(s) | 1015 |
| XceI | RCATGY | 1 cut(s) | 720 |
| XhoI | CTCGAG | 1 cut(s) | 137 |
| XmiI | GTMKAC | 1 cut(s) | 1100 |
| Zsp2I | ATGCAT | 1 cut(s) | 605 |