Rh6DG430800

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011653
Physical Location & Seq
Reverse (-)
61009038 .. 61011748
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG430800.1

Sequence Viewer

Length: 1149 bp
ATGGATCTTGAGCCCGGAACCATGGACAGCATCAGATCCGGCCCCTTCGGACAGATCTTCCGCCCCGATAACTTCGTCTTCGGACAGTCCGGCGCCGGTAATAATTGGGCCAAAGGTCACTATACCGAAGGAGCTGAGCTGATCGATTCCGTTCTCGATGTTGTTCGCAAGGAGGCGGAGAACTGCGATTGCCTTCAAGGTTTCCAGGTGTGCCACTCACTTGGAGGAGGAACTGGTTCTGGCATGGGAACCCTTCTGATATCGAAGATCAGAGAGGAATACCCTGACAGGATGATGCTTACATTCTCTGTGTTCCCATCTCCAAAGGTCTCGGACACTGTTGTGGAGCCATACAATGCCACTCTTTCAGTCCACCAGTTGGTAGAGAATGCTGATGAATGCATGGTTCTAGATAACGAGGCTCTTTATGACATTTGCTTCAGAACCTTAAAACTCAGTACCCCAAGCTTTGGTGACCTGAACCATTTGATATCTGCAACTATGAGTGGTGTTACTTGCTGCCTAAGGTTCCCTGGTCAGCTGAACTCTGATCTCCGGAAGCTGGCTGTCAATCTCATTCCATTCCCACGTCTTCACTTCTTCATGGTTGGGTTCGCCCCACTCACATCTCGCGGTTCCCAGCAGTACATCTCCCTCACCGTGCCAGAGCTGACTCAGCAAATGTGGGATGCCAAGAACATGATGTGTGCTGCTGACCCTCGCCACGGAAGGTACCTGACAGCCTCGGCCATGTTCAGGGGTAAGATGAGCACCAAAGAGGTGGATGAACAGATGATCAATGTGCAGAACAAGAACTCTTCCTACTTTGTTGAGTGGATTCCTCACAATGTGAAGTCCAGCGTGTGCGACATCCCTCCCCTGGGATTGAAAATGGCATCAACCTTTGTTGGTAACTCTACCTCAATCCAGGAGATGTTCAGGAGAGTGAGCGAGCAGTTCACAGCCATGTTCAGGCGCAAGGCCTTTTTGCATTGGTATACCGGAGAAGGTATGGACGAGATGGAGTTCACCGAAGCTGAGAGTAACATGAATGATTTGGTTGCCGAGTACCAGCAATACCAGGATGCTACAGTTGAGGATGAAGGTGAATATGAGGAGGATGGTGTTGAAGGTGAGTATGAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

382

Amino Acids

42.89

Weight (kDa)

4.68

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 1 - 146 1.4e-45 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 196 - 317 4.2e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 732
AccB1I GGYRCC 2 cut(s) 92, 732
AccB7I CCANNNNNTGG 2 cut(s) 379, 470
AccI GTMKAC 1 cut(s) 998
AccII CGCG 1 cut(s) 633
AccIII TCCGGA 1 cut(s) 555
AciI CCGC 3 cut(s) 61, 176, 633
AclWI GGATC 2 cut(s) 12, 30
AcoI YGGCCR 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 424
AcyI GRCGYC 1 cut(s) 93
AdeI CACNNNGTG 1 cut(s) 850
AfaI GTAC 4 cut(s) 460, 647, 734, 1070
AfiI CCNNNNNNNGG 8 cut(s) 379, 470, 562, 725, 756, 880, 881, 972
AgsI TTSAA 3 cut(s) 197, 889, 1130
AjiI CACGTC 1 cut(s) 590
AjnI CCWGG 5 cut(s) 204, 532, 879, 927, 1080
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 7 cut(s) 134, 139, 468, 541, 562, 670, 1037
AluI AGCT 7 cut(s) 134, 139, 468, 541, 562, 670, 1037
Alw21I GWGCWC 1 cut(s) 773
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 2 cut(s) 12, 30
Aor13HI TCCGGA 1 cut(s) 555
AoxI GGCC 4 cut(s) 40, 108, 747, 981
ApeKI GCWGC 2 cut(s) 519, 710
Asp700I GAANNNNTTC 1 cut(s) 235
Asp718I GGTACC 1 cut(s) 732
AspLEI GCGC 2 cut(s) 95, 978
AspS9I GGNCC 2 cut(s) 41, 108
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 5 cut(s) 485, 649, 1021, 1118, 1145
AxyI CCTNAGG 1 cut(s) 524
BanI GGYRCC 2 cut(s) 92, 732
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 2 cut(s) 70, 584
Bbv12I GWGCWC 1 cut(s) 773
BbvI GCAGC 2 cut(s) 506, 697
BccI CCATC 3 cut(s) 325, 1015, 1115
BciT130I CCWGG 5 cut(s) 206, 534, 881, 929, 1082
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 1089
BfoI RGCGCY 1 cut(s) 96
BglII AGATCT 1 cut(s) 54
BisI GCNGC 2 cut(s) 520, 711
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 2 cut(s) 521, 712
Bme1390I CCNGG 6 cut(s) 15, 206, 534, 881, 929, 1082
BmgBI CACGTC 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 41, 108
BmiI GGNNCC 8 cut(s) 19, 43, 94, 250, 348, 530, 637, 734
BmrFI CCNGG 6 cut(s) 15, 206, 534, 881, 929, 1082
BmsI GCATC 5 cut(s) 39, 285, 679, 905, 1075
BpiI GAAGAC 2 cut(s) 70, 584
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 6 cut(s) 21, 532, 724, 744, 879, 880
BsaWI WCCGGW 2 cut(s) 555, 1001
Bsc4I CCNNNNNNNGG 8 cut(s) 379, 470, 562, 725, 756, 880, 881, 972
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 238, 376
Bse21I CCTNAGG 1 cut(s) 524
BseAI TCCGGA 1 cut(s) 555
BseBI CCWGG 5 cut(s) 206, 534, 881, 929, 1082
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 6 cut(s) 21, 532, 724, 744, 879, 880
BseGI GGATG 7 cut(s) 297, 694, 790, 870, 1090, 1105, 1126
BseLI CCNNNNNNNGG 8 cut(s) 379, 470, 562, 725, 756, 880, 881, 972
BseMII CTCAG 4 cut(s) 126, 469, 689, 1029
BseNI ACTGG 2 cut(s) 238, 376
BseRI GAGGAG 2 cut(s) 240, 1130
BseXI GCAGC 2 cut(s) 506, 697
BseYI CCCAGC 1 cut(s) 639
BsgI GTGCAG 1 cut(s) 824
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 4 cut(s) 42, 110, 749, 983
BshNI GGYRCC 2 cut(s) 92, 732
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 773
BsiSI CCGG 6 cut(s) 15, 39, 90, 96, 556, 1002
BslI CCNNNNNNNGG 8 cut(s) 379, 470, 562, 725, 756, 880, 881, 972
BsmAI GTCTC 1 cut(s) 334
BsmI GAATGC 2 cut(s) 394, 404
BsnI GGCC 4 cut(s) 42, 110, 749, 983
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 2 cut(s) 15, 773
Bsp13I TCCGGA 1 cut(s) 555
Bsp143I GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
Bsp1720I GCTNAGC 1 cut(s) 135
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 61, 176, 633
BspANI GGCC 4 cut(s) 42, 110, 749, 983
BspCNI CTCAG 4 cut(s) 127, 468, 688, 1030
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 555
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 8 cut(s) 19, 43, 94, 250, 348, 530, 637, 734
BspPI GGATC 2 cut(s) 12, 30
BspT107I GGYRCC 2 cut(s) 92, 732
BspTNI GGTCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 2 cut(s) 238, 376
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 6 cut(s) 21, 532, 724, 744, 879, 880
BssMI GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
BssNAI GTATAC 1 cut(s) 999
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 21
Bst1107I GTATAC 1 cut(s) 999
Bst2UI CCWGG 5 cut(s) 206, 534, 881, 929, 1082
Bst4CI ACNGT 4 cut(s) 87, 340, 661, 1093
Bst6I CTCTTC 1 cut(s) 823
BstACI GRCGYC 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 564, 953
BstDEI CTNAG 5 cut(s) 135, 455, 524, 675, 1038
BstDSI CCRYGG 2 cut(s) 21, 724
BstEII GGTNACC 1 cut(s) 473
BstF5I GGATG 7 cut(s) 297, 694, 790, 870, 1090, 1105, 1126
BstFNI CGCG 1 cut(s) 633
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 978
BstKTI GATC 7 cut(s) 7, 38, 57, 144, 270, 553, 798
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
BstMWI GCNNNNNNNGC 1 cut(s) 676
BstNI CCWGG 5 cut(s) 206, 534, 881, 929, 1082
BstPI GGTNACC 1 cut(s) 473
BstSCI CCNGG 6 cut(s) 13, 204, 532, 879, 927, 1080
BstSFI CTRYAG 1 cut(s) 1089
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 2 cut(s) 506, 697
BstV2I GAAGAC 2 cut(s) 70, 584
BstX2I RGATCY 3 cut(s) 4, 35, 54
BstXI CCANNNNNNTGG 2 cut(s) 221, 781
BstYI RGATCY 3 cut(s) 4, 35, 54
BstZ17I GTATAC 1 cut(s) 999
Bsu15I ATCGAT 1 cut(s) 144
Bsu36I CCTNAGG 1 cut(s) 524
BsuRI GGCC 4 cut(s) 42, 110, 749, 983
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 21, 724
BtrI CACGTC 1 cut(s) 590
BtsCI GGATG 7 cut(s) 297, 694, 790, 870, 1090, 1105, 1126
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 564, 953
CfoI GCGC 2 cut(s) 95, 978
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 2 cut(s) 41, 108
ClaI ATCGAT 1 cut(s) 144
Csp6I GTAC 4 cut(s) 459, 646, 733, 1069
CviAII CATG 8 cut(s) 22, 244, 403, 604, 700, 751, 967, 1048
CviQI GTAC 4 cut(s) 459, 646, 733, 1069
DdeI CTNAG 5 cut(s) 135, 455, 524, 675, 1038
DinI GGCGCC 1 cut(s) 94
DpnI GATC 7 cut(s) 6, 37, 56, 143, 269, 552, 797
DpnII GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
DraIII CACNNNGTG 1 cut(s) 850
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 823
EciI GGCGGA 2 cut(s) 50, 191
Eco130I CCWWGG 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 983
Eco24I GRGCYC 1 cut(s) 15
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 2 cut(s) 261, 492
Eco57I CTGAAG 1 cut(s) 424
Eco81I CCTNAGG 1 cut(s) 524
Eco91I GGTNACC 1 cut(s) 473
EcoO65I GGTNACC 1 cut(s) 473
EcoRII CCWGG 5 cut(s) 204, 532, 879, 927, 1080
EcoRV GATATC 2 cut(s) 261, 492
EcoT14I CCWWGG 1 cut(s) 21
EcoT22I ATGCAT 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 15
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 8 cut(s) 25, 247, 406, 607, 703, 754, 970, 1051
FatI CATG 8 cut(s) 21, 243, 402, 603, 699, 750, 966, 1047
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 998
Fnu4HI GCNGC 2 cut(s) 520, 711
FokI GGATG 7 cut(s) 304, 701, 797, 857, 1097, 1112, 1133
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 520, 711
FspBI CTAG 1 cut(s) 410
GlaI GCGC 2 cut(s) 94, 977
GluI GCNGC 2 cut(s) 520, 711
GsaI CCCAGC 1 cut(s) 643
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 42, 110, 749, 983
HapII CCGG 6 cut(s) 15, 39, 90, 96, 556, 1002
HhaI GCGC 2 cut(s) 95, 978
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 8 cut(s) 25, 247, 406, 607, 703, 754, 970, 1051
Hin6I GCGC 2 cut(s) 93, 976
HinP1I GCGC 2 cut(s) 93, 976
HindIII AAGCTT 1 cut(s) 466
HinfI GANTC 3 cut(s) 146, 673, 838
HpaII CCGG 6 cut(s) 15, 39, 90, 96, 556, 1002
HphI GGTGA 5 cut(s) 485, 649, 1021, 1118, 1145
Hpy166II GTNNAC 4 cut(s) 373, 960, 999, 1029
Hpy188I TCNGA 8 cut(s) 35, 50, 83, 258, 272, 334, 443, 550
Hpy188III TCNNGA 5 cut(s) 8, 155, 410, 556, 940
Hpy8I GTNNAC 4 cut(s) 373, 960, 999, 1029
HpyAV CCTTC 8 cut(s) 55, 122, 203, 263, 723, 1001, 1097, 1124
HpyCH4III ACNGT 4 cut(s) 87, 340, 661, 1093
HpyCH4IV ACGT 1 cut(s) 589
HpyCH4V TGCA 4 cut(s) 402, 497, 805, 991
HpyF10VI GCNNNNNNNGC 1 cut(s) 676
HpyF3I CTNAG 5 cut(s) 135, 455, 524, 675, 1038
HpySE526I ACGT 1 cut(s) 589
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 8 cut(s) 25, 247, 406, 607, 703, 754, 970, 1051
HspAI GCGC 2 cut(s) 93, 976
KasI GGCGCC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 555
KpnI GGTACC 1 cut(s) 736
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
LmnI GCTCC 2 cut(s) 131, 346
Lsp1109I GCAGC 2 cut(s) 506, 697
LweI GCATC 5 cut(s) 39, 285, 679, 905, 1075
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 589
MaeIII GTNAC 5 cut(s) 116, 473, 511, 911, 1043
MalI GATC 7 cut(s) 6, 37, 56, 143, 269, 552, 797
MboI GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
MboII GAAGA 6 cut(s) 49, 70, 277, 584, 592, 810
MflI RGATCY 3 cut(s) 4, 35, 54
MhlI GDGCHC 2 cut(s) 15, 773
MluCI AATT 1 cut(s) 103
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 667
Mph1103I ATGCAT 1 cut(s) 404
MroI TCCGGA 1 cut(s) 555
MroXI GAANNNNTTC 1 cut(s) 235
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 2 cut(s) 341, 965
MspA1I CMGCKG 1 cut(s) 541
MspI CCGG 6 cut(s) 15, 39, 90, 96, 556, 1002
MspR9I CCNGG 6 cut(s) 15, 206, 534, 881, 929, 1082
Mva1269I GAATGC 2 cut(s) 394, 404
MvaI CCWGG 5 cut(s) 206, 534, 881, 929, 1082
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 1 cut(s) 676
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 15
NcoI CCATGG 1 cut(s) 21
NdeII GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
NlaIII CATG 8 cut(s) 25, 247, 406, 607, 703, 754, 970, 1051
NlaIV GGNNCC 8 cut(s) 19, 43, 94, 250, 348, 530, 637, 734
NmeAIII GCCGAG 2 cut(s) 725, 1090
NmuCI GTSAC 2 cut(s) 116, 473
NsiI ATGCAT 1 cut(s) 404
OliI CACNNNNGTG 1 cut(s) 341
PasI CCCWGGG 1 cut(s) 880
PceI AGGCCT 1 cut(s) 983
PctI GAATGC 2 cut(s) 394, 404
PdmI GAANNNNTTC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 146, 838
PflMI CCANNNNNTGG 2 cut(s) 379, 470
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 2 cut(s) 521, 712
PleI GAGTC 1 cut(s) 667
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 667
Psp6I CCWGG 5 cut(s) 204, 532, 879, 927, 1080
PspEI GGTNACC 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 5 cut(s) 204, 532, 879, 927, 1080
PspN4I GGNNCC 8 cut(s) 19, 43, 94, 250, 348, 530, 637, 734
PspPI GGNCC 2 cut(s) 41, 108
PsrI GAACNNNNNNTAC 2 cut(s) 806, 838
PsuI RGATCY 3 cut(s) 4, 35, 54
PvuII CAGCTG 1 cut(s) 541
RsaI GTAC 4 cut(s) 460, 647, 734, 1070
RsaNI GTAC 4 cut(s) 459, 646, 733, 1069
RseI CAYNNNNRTG 2 cut(s) 341, 965
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 2 cut(s) 520, 711
Sau3AI GATC 7 cut(s) 4, 35, 54, 141, 267, 550, 795
Sau96I GGNCC 2 cut(s) 41, 108
SchI GAGTC 1 cut(s) 667
ScrFI CCNGG 6 cut(s) 15, 206, 534, 881, 929, 1082
SduI GDGCHC 2 cut(s) 15, 773
SfaNI GCATC 5 cut(s) 39, 285, 679, 905, 1075
SfcI CTRYAG 1 cut(s) 1089
SfoI GGCGCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 341, 965
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 1 cut(s) 103
SseBI AGGCCT 1 cut(s) 983
SsiI CCGC 3 cut(s) 61, 176, 633
SspDI GGCGCC 1 cut(s) 92
SspMI CTAG 1 cut(s) 410
StuI AGGCCT 1 cut(s) 983
StyD4I CCNGG 6 cut(s) 13, 204, 532, 879, 927, 1080
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 4 cut(s) 87, 340, 661, 1093
TaiI ACGT 1 cut(s) 592
TaqI TCGA 3 cut(s) 144, 156, 263
TasI AATT 1 cut(s) 103
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 2 cut(s) 146, 838
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 2 cut(s) 116, 473
TseI GCWGC 2 cut(s) 519, 710
Tsp45I GTSAC 2 cut(s) 116, 473
TspDTI ATGAA 5 cut(s) 411, 592, 801, 1064, 1116
TspGWI ACGGA 2 cut(s) 139, 741
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 2 cut(s) 379, 470
XbaI TCTAGA 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 998
XmnI GAANNNNTTC 1 cut(s) 235
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.