Rh6AG431600

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011653
Physical Location & Seq
Reverse (-)
62197174 .. 62200225
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG431600.1

Sequence Viewer

Length: 987 bp
ATGATGATTGATTATTCCTTTTCTGGTCATGTATCAGGTTTCCAAGTGTGCCACTCACTTGGAGGAGGAACTGGTTCTGGCATGGGAACCCTTCTGATATCGAAGATCAGAGAGGAGTACCCTGACAGGATGATGCTTACATTCTCTGTGTTCCCATCTCCAAAGGTCTCGGACACTGTTGTGGAACCATACAATGCCACTCTTTCAGTGCACCAGTTGGTGGAGAATGCTGATGAATGCATGGTTCTAGATAACGAGGCTCTTTATGACATTTGCTTCAGAACCCTAAAACTCAGTACCCCAAGCTTCGGTGACCTAAACCATTTGATATCTGCAACTATGAGTGGTGTTACTTGCTGCCTAAGGTTCCCTGGTCAGCTGAACTCTGATCTCCGGAAGCTGGCTGTCAATCTCATTCCATTCCCACGTCTTCACTTCTTCATGGTTGGGTTTGCCCCACTCACATCTCGTGGTTCCCAGCAGTACATCTCCCTCACCGTGCCAGAGCTGACTCAGCAAATGTGGGATGCCAAGAACATGATGTGTGCTGCTGACCCTCGCCACGGAAGGTACCTGACAGCCTCGGCCATGTTCAGGGGTAAGATGAGCACCAAAGAGGTGGACGAACAGATGATCAATGTGCAGAACAAGAACTCTTCCTACTTTGTTGAGTGGATTCCTCACAATGTGAAGTCCAGCGTTTGCGACATCCCCCCCCTGGGATTGAAAATGGCATCCACCTTTGTTGGTAACTCTACCTCAATCCAGGAGATGTTCAGGAGAGTGAGCGAGCAGTTCACAGCCATGTTCAGGCGCAAGGCCTTTTTGCATTGGTATACCGGAGAAGGTATGGACGAGATGGAGTTCACCGAAGCTGAGAGTAACATGAATGATTTGGTTGCCGAGTACCAGCAGTACCAGGATGCTACGGTTGAGGATGAAGGTGAATATGAAGAGGATGGTGTTGAAGGTGAGTATGAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

328

Amino Acids

37.03

Weight (kDa)

4.78

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 10 - 92 5e-26 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 142 - 263 3e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 570
AccB7I CCANNNNNTGG 1 cut(s) 220
AccI GTMKAC 1 cut(s) 836
AccIII TCCGGA 1 cut(s) 393
AcoI YGGCCR 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 262
AdeI CACNNNGTG 1 cut(s) 688
AfaI GTAC 6 cut(s) 119, 298, 485, 572, 908, 917
AfiI CCNNNNNNNGG 8 cut(s) 220, 308, 400, 563, 594, 718, 719, 810
AgsI TTSAA 2 cut(s) 727, 968
AjiI CACGTC 1 cut(s) 428
AjnI CCWGG 4 cut(s) 370, 717, 765, 918
AleI CACNNNNGTG 1 cut(s) 179
AluBI AGCT 5 cut(s) 306, 379, 400, 508, 875
AluI AGCT 5 cut(s) 306, 379, 400, 508, 875
Alw21I GWGCWC 2 cut(s) 213, 611
Alw26I GTCTC 1 cut(s) 172
Alw44I GTGCAC 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 393
AoxI GGCC 2 cut(s) 585, 819
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 357, 548
Asp700I GAANNNNTTC 1 cut(s) 73
Asp718I GGTACC 1 cut(s) 570
AspLEI GCGC 1 cut(s) 816
AsuHPI GGTGA 5 cut(s) 323, 487, 859, 956, 983
AxyI CCTNAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 213
BanI GGYRCC 1 cut(s) 570
BauI CACGAG 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 2 cut(s) 213, 611
BbvI GCAGC 2 cut(s) 344, 535
BccI CCATC 3 cut(s) 163, 853, 953
BciT130I CCWGG 4 cut(s) 372, 719, 767, 920
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 248
BisI GCNGC 2 cut(s) 358, 549
BlsI GCNGC 2 cut(s) 359, 550
Bme1390I CCNGG 4 cut(s) 372, 719, 767, 920
BmgBI CACGTC 1 cut(s) 428
BmiI GGNNCC 5 cut(s) 88, 186, 368, 475, 572
BmrFI CCNGG 4 cut(s) 372, 719, 767, 920
BmsI GCATC 4 cut(s) 123, 517, 743, 913
BpiI GAAGAC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 5 cut(s) 370, 562, 582, 717, 718
BsaWI WCCGGW 2 cut(s) 393, 839
Bsc4I CCNNNNNNNGG 8 cut(s) 220, 308, 400, 563, 594, 718, 719, 810
Bse1I ACTGG 2 cut(s) 76, 214
Bse21I CCTNAGG 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 393
BseBI CCWGG 4 cut(s) 372, 719, 767, 920
BseDI CCNNGG 5 cut(s) 370, 562, 582, 717, 718
BseGI GGATG 7 cut(s) 135, 532, 708, 734, 928, 943, 964
BseLI CCNNNNNNNGG 8 cut(s) 220, 308, 400, 563, 594, 718, 719, 810
BseMII CTCAG 3 cut(s) 307, 527, 867
BseNI ACTGG 2 cut(s) 76, 214
BseRI GAGGAG 2 cut(s) 78, 128
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 2 cut(s) 344, 535
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 662
BshFI GGCC 2 cut(s) 587, 821
BshNI GGYRCC 1 cut(s) 570
BsiHKAI GWGCWC 2 cut(s) 213, 611
BsiSI CCGG 2 cut(s) 394, 840
BslI CCNNNNNNNGG 8 cut(s) 220, 308, 400, 563, 594, 718, 719, 810
BsmAI GTCTC 1 cut(s) 172
BsmI GAATGC 2 cut(s) 232, 242
BsnI GGCC 2 cut(s) 587, 821
Bso31I GGTCTC 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 213, 611
Bsp13I TCCGGA 1 cut(s) 393
Bsp143I GATC 3 cut(s) 105, 388, 633
BspANI GGCC 2 cut(s) 587, 821
BspCNI CTCAG 3 cut(s) 306, 526, 868
BspEI TCCGGA 1 cut(s) 393
BspLI GGNNCC 5 cut(s) 88, 186, 368, 475, 572
BspT107I GGYRCC 1 cut(s) 570
BspTNI GGTCTC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 76, 214
BssECI CCNNGG 5 cut(s) 370, 562, 582, 717, 718
BssMI GATC 3 cut(s) 105, 388, 633
BssNAI GTATAC 1 cut(s) 837
BssSI CACGAG 1 cut(s) 468
Bst1107I GTATAC 1 cut(s) 837
Bst2BI CACGAG 1 cut(s) 468
Bst2UI CCWGG 4 cut(s) 372, 719, 767, 920
Bst4CI ACNGT 3 cut(s) 178, 499, 931
Bst6I CTCTTC 2 cut(s) 661, 948
BstC8I GCNNGC 2 cut(s) 402, 791
BstDEI CTNAG 4 cut(s) 293, 362, 513, 876
BstDSI CCRYGG 1 cut(s) 562
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 7 cut(s) 135, 532, 708, 734, 928, 943, 964
BstHHI GCGC 1 cut(s) 816
BstKTI GATC 3 cut(s) 108, 391, 636
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 3 cut(s) 105, 388, 633
BstMWI GCNNNNNNNGC 1 cut(s) 514
BstNI CCWGG 4 cut(s) 372, 719, 767, 920
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 370, 717, 765, 918
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 344, 535
BstV2I GAAGAC 1 cut(s) 422
BstXI CCANNNNNNTGG 2 cut(s) 59, 619
BstZ17I GTATAC 1 cut(s) 837
Bsu36I CCTNAGG 1 cut(s) 362
BsuRI GGCC 2 cut(s) 587, 821
BtgI CCRYGG 1 cut(s) 562
BtrI CACGTC 1 cut(s) 428
BtsCI GGATG 7 cut(s) 135, 532, 708, 734, 928, 943, 964
BtsIMutI CAGTG 2 cut(s) 174, 213
Cac8I GCNNGC 2 cut(s) 402, 791
CfoI GCGC 1 cut(s) 816
Csp6I GTAC 6 cut(s) 118, 297, 484, 571, 907, 916
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 8 cut(s) 29, 82, 241, 442, 538, 589, 805, 886
CviQI GTAC 6 cut(s) 118, 297, 484, 571, 907, 916
DdeI CTNAG 4 cut(s) 293, 362, 513, 876
DpnI GATC 3 cut(s) 107, 390, 635
DpnII GATC 3 cut(s) 105, 388, 633
DraIII CACNNNGTG 1 cut(s) 688
EaeI YGGCCR 1 cut(s) 585
Eam1104I CTCTTC 2 cut(s) 661, 948
EarI CTCTTC 2 cut(s) 661, 948
Eco147I AGGCCT 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 172
Eco32I GATATC 2 cut(s) 99, 330
Eco57I CTGAAG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 311
EcoRII CCWGG 4 cut(s) 370, 717, 765, 918
EcoRV GATATC 2 cut(s) 99, 330
EcoT22I ATGCAT 1 cut(s) 242
FaeI CATG 8 cut(s) 32, 85, 244, 445, 541, 592, 808, 889
FatI CATG 8 cut(s) 28, 81, 240, 441, 537, 588, 804, 885
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 836
Fnu4HI GCNGC 2 cut(s) 358, 549
FokI GGATG 7 cut(s) 142, 539, 695, 721, 935, 950, 971
Fsp4HI GCNGC 2 cut(s) 358, 549
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 815
GluI GCNGC 2 cut(s) 358, 549
GsaI CCCAGC 1 cut(s) 481
HaeIII GGCC 2 cut(s) 587, 821
HapII CCGG 2 cut(s) 394, 840
HhaI GCGC 1 cut(s) 816
Hin1II CATG 8 cut(s) 32, 85, 244, 445, 541, 592, 808, 889
Hin6I GCGC 1 cut(s) 814
HinP1I GCGC 1 cut(s) 814
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 2 cut(s) 511, 676
HpaII CCGG 2 cut(s) 394, 840
HphI GGTGA 5 cut(s) 323, 487, 859, 956, 983
Hpy166II GTNNAC 5 cut(s) 211, 622, 798, 837, 867
Hpy188I TCNGA 5 cut(s) 96, 110, 172, 281, 388
Hpy188III TCNNGA 3 cut(s) 248, 394, 778
Hpy8I GTNNAC 5 cut(s) 211, 622, 798, 837, 867
HpyAV CCTTC 5 cut(s) 101, 561, 839, 935, 962
HpyCH4III ACNGT 3 cut(s) 178, 499, 931
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 5 cut(s) 211, 240, 335, 643, 829
HpyF10VI GCNNNNNNNGC 1 cut(s) 514
HpyF3I CTNAG 4 cut(s) 293, 362, 513, 876
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 8 cut(s) 32, 85, 244, 445, 541, 592, 808, 889
HspAI GCGC 1 cut(s) 814
Kpn2I TCCGGA 1 cut(s) 393
KpnI GGTACC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 3 cut(s) 105, 388, 633
Lsp1109I GCAGC 2 cut(s) 344, 535
LweI GCATC 4 cut(s) 123, 517, 743, 913
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 4 cut(s) 311, 349, 749, 881
MalI GATC 3 cut(s) 107, 390, 635
MboI GATC 3 cut(s) 105, 388, 633
MboII GAAGA 5 cut(s) 115, 422, 430, 648, 965
MhlI GDGCHC 2 cut(s) 213, 611
MlyI GAGTC 1 cut(s) 505
Mph1103I ATGCAT 1 cut(s) 242
MroI TCCGGA 1 cut(s) 393
MroXI GAANNNNTTC 1 cut(s) 73
MslI CAYNNNNRTG 2 cut(s) 179, 803
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 2 cut(s) 394, 840
MspR9I CCNGG 4 cut(s) 372, 719, 767, 920
Mva1269I GAATGC 2 cut(s) 232, 242
MvaI CCWGG 4 cut(s) 372, 719, 767, 920
MwoI GCNNNNNNNGC 1 cut(s) 514
NdeII GATC 3 cut(s) 105, 388, 633
NlaIII CATG 8 cut(s) 32, 85, 244, 445, 541, 592, 808, 889
NlaIV GGNNCC 5 cut(s) 88, 186, 368, 475, 572
NmeAIII GCCGAG 2 cut(s) 563, 928
NmuCI GTSAC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 242
OliI CACNNNNGTG 1 cut(s) 179
PasI CCCWGGG 1 cut(s) 718
PceI AGGCCT 1 cut(s) 821
PctI GAATGC 2 cut(s) 232, 242
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 676
PflMI CCANNNNNTGG 1 cut(s) 220
PfoI TCCNGGA 1 cut(s) 765
PkrI GCNGC 2 cut(s) 359, 550
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Psp6I CCWGG 4 cut(s) 370, 717, 765, 918
PspEI GGTNACC 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 4 cut(s) 370, 717, 765, 918
PspN4I GGNNCC 5 cut(s) 88, 186, 368, 475, 572
PsrI GAACNNNNNNTAC 2 cut(s) 644, 676
PvuII CAGCTG 1 cut(s) 379
RsaI GTAC 6 cut(s) 119, 298, 485, 572, 908, 917
RsaNI GTAC 6 cut(s) 118, 297, 484, 571, 907, 916
RseI CAYNNNNRTG 2 cut(s) 179, 803
SatI GCNGC 2 cut(s) 358, 549
Sau3AI GATC 3 cut(s) 105, 388, 633
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 4 cut(s) 372, 719, 767, 920
SduI GDGCHC 2 cut(s) 213, 611
SfaNI GCATC 4 cut(s) 123, 517, 743, 913
SmiMI CAYNNNNRTG 2 cut(s) 179, 803
SseBI AGGCCT 1 cut(s) 821
SspMI CTAG 1 cut(s) 248
StuI AGGCCT 1 cut(s) 821
StyD4I CCNGG 4 cut(s) 370, 717, 765, 918
TaaI ACNGT 3 cut(s) 178, 499, 931
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 101
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 1 cut(s) 676
TscAI CASTG 2 cut(s) 181, 213
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 357, 548
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 5 cut(s) 249, 430, 902, 954, 966
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 2 cut(s) 181, 213
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 209
XbaI TCTAGA 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 836
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.