Rorug06G0318000

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011653
Physical Location & Seq
Reverse (-)
46995622 .. 46997918
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0318000.1

Sequence Viewer

Length: 318 bp
ATGGCGTCCGATACGGCGTCGTCTGTGGACGAACAACAGGCACAGCTTGTCGCTGTTACTCCAACTGACAACAGAGGAAGGCATCGGGTTCTTGCCGAGCTCAACCGACTCGAACAAGAACTCAAATTCTTGCAGGAAGAGTTGGGAGAGCTTGAACAAACTGAAAACGTTTCCACCATATGTTCTGAATTGCTGCCCTATACCGAAGGCCGGCCGGATCCTCTACTTCCACTAACAAATGGGCCTATAAATGTAATATGGGATCGATGGTTTGAAGGACCTCAAAACTCACAAAGTTGCAGTTGCTTGATACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

105

Amino Acids

11.8

Weight (kDa)

4.3

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 33 - 105 2.8e-12 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 168
AclWI GGATC 3 cut(s) 212, 225, 270
AcoI YGGCCR 1 cut(s) 212
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 2 cut(s) 5, 17
AfiI CCNNNNNNNGG 1 cut(s) 210
AgsI TTSAA 2 cut(s) 155, 275
AluBI AGCT 3 cut(s) 46, 100, 151
AluI AGCT 3 cut(s) 46, 100, 151
Alw21I GWGCWC 1 cut(s) 102
AlwI GGATC 3 cut(s) 212, 225, 270
AoxI GGCC 3 cut(s) 208, 212, 242
ApeKI GCWGC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 242, 278
AvaII GGWCC 1 cut(s) 278
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 180
BccI CCATC 1 cut(s) 261
BceAI ACGGC 1 cut(s) 30
BisI GCNGC 1 cut(s) 194
BlsI GCNGC 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 242, 278
BmiI GGNNCC 1 cut(s) 219
BmsI GCATC 1 cut(s) 91
Bsa29I ATCGAT 1 cut(s) 265
BsaHI GRCGYC 2 cut(s) 5, 17
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse118I RCCGGY 1 cut(s) 210
BseCI ATCGAT 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 3 cut(s) 210, 214, 244
BshVI ATCGAT 1 cut(s) 265
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 211, 215
BslI CCNNNNNNNGG 1 cut(s) 210
BsnI GGCC 3 cut(s) 210, 214, 244
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 217, 262
BspANI GGCC 3 cut(s) 210, 214, 244
BspDI ATCGAT 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 3 cut(s) 212, 225, 270
BsrFI RCCGGY 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 210
BssMI GATC 2 cut(s) 217, 262
BssNI GRCGYC 2 cut(s) 5, 17
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 2 cut(s) 5, 17
BstC8I GCNNGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 220, 265
BstMBI GATC 2 cut(s) 217, 262
BstMCI CGRYCG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 265
BsuRI GGCC 3 cut(s) 210, 214, 244
BsuTUI ATCGAT 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 242, 278
ClaI ATCGAT 1 cut(s) 265
CseI GACGC 1 cut(s) 6
CviJI RGCY 6 cut(s) 46, 100, 151, 210, 214, 244
CviKI_1 RGCY 6 cut(s) 46, 100, 151, 210, 214, 244
DpnI GATC 2 cut(s) 219, 264
DpnII GATC 2 cut(s) 217, 262
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 278
Eco52I CGGCCG 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 102
FaiI YATR 5 cut(s) 179, 181, 201, 248, 259
FauNDI CATATG 1 cut(s) 179
Fnu4HI GCNGC 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 102
FseI GGCCGGCC 1 cut(s) 214
Fsp4HI GCNGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 194
HaeIII GGCC 3 cut(s) 210, 214, 244
HapII CCGG 2 cut(s) 211, 215
HgaI GACGC 1 cut(s) 6
Hin1I GRCGYC 2 cut(s) 5, 17
HinfI GANTC 1 cut(s) 108
HpaII CCGG 2 cut(s) 211, 215
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 2 cut(s) 10, 187
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 3 cut(s) 72, 200, 269
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 2 cut(s) 133, 300
HpySE526I ACGT 1 cut(s) 168
Hsp92I GRCGYC 2 cut(s) 5, 17
KroI GCCGGC 1 cut(s) 210
KroNI GCCGGC 1 cut(s) 212
Kzo9I GATC 2 cut(s) 217, 262
LpnPI CCDG 4 cut(s) 23, 119, 224, 228
Lsp1109I GCAGC 1 cut(s) 180
LweI GCATC 1 cut(s) 91
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 55
MalI GATC 2 cut(s) 219, 264
MboI GATC 2 cut(s) 217, 262
MboII GAAGA 1 cut(s) 149
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 2 cut(s) 125, 188
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 3 cut(s) 68, 231, 291
MroNI GCCGGC 1 cut(s) 210
MspI CCGG 2 cut(s) 211, 215
NaeI GCCGGC 1 cut(s) 212
NdeI CATATG 1 cut(s) 179
NdeII GATC 2 cut(s) 217, 262
NgoMIV GCCGGC 1 cut(s) 210
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 195
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
PpuMI RGGWCCY 1 cut(s) 278
Psp124BI GAGCTC 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 2 cut(s) 242, 278
PspPPI RGGWCCY 1 cut(s) 278
PsuI RGATCY 1 cut(s) 217
RigI GGCCGGCC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 102
SatI GCNGC 1 cut(s) 194
Sau3AI GATC 2 cut(s) 217, 262
Sau96I GGNCC 2 cut(s) 242, 278
SchI GAGTC 1 cut(s) 102
SduI GDGCHC 1 cut(s) 102
SetI ASST 5 cut(s) 48, 102, 153, 171, 283
SfaNI GCATC 1 cut(s) 91
SinI GGWCC 1 cut(s) 278
Sse9I AATT 2 cut(s) 125, 188
SstI GAGCTC 1 cut(s) 102
TaaI ACNGT 1 cut(s) 315
TaiI ACGT 1 cut(s) 171
TaqI TCGA 2 cut(s) 111, 265
TasI AATT 2 cut(s) 125, 188
TseI GCWGC 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.