MD15G1011800.v1.1

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011653
Physical Location & Seq
Forward (+)
641557 .. 644352
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1011800.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGAGAGAGATTCTGCACATCCAGGGAGGCCAATGCGGCAACCAGATCGGCTCCAAGTTCTGGGAGGTCATCTGCGACGAGCACGGCGTCGACCCCACGGGAAGGTACCGCGGAGACGCCGGCTCCGATCTCCAGCTCGAGAGGATCAACGTCTACTACAATGAGGCCTCCGGCGGTAGGTACGTCCCCCGCGCCGTACTCATGGATCTTGAGCCCGGTACCATGGACAGCATCCGATCCGGCCCCTTCGGCCAGATCTTCCGCCCTGACAACTTCGTCTTCGGCCAGTCCGGCGCCGGCAACAATTGGGCGAAAGGACACTATACCGAAGGTGCTGAGTTGATCGATTCCGTTCTCGATGTTGTTCGCAAGGAGGCCGAGAATTGTGATTGCCTTCAAGGTTTTCAGGTGTGCCACTCTCTTGGAGGAGGAACTGGTTCTGGAATGGGAACCCTTCTCATATCAAAGATTAGAGAGGAATACCCTGACAGGATGCTGCTCACGTTCTCTGTTTTCCCTTCACCAAAGGTCTCAGACACCGTTGTGGAACCATACAATGCCACTCTTTCGGTGCACCAGCTGGTTGAGAACGCAGATGAATGCATGGTTCTTGATAACGAAGCACTTTATGACATTTGCTTCAGGACCCTAAAGCTCAGCACCCCAAGCTTTGGTGACCTGAACCATTTGATATCCGCAACCATGAGCGGTGTAACATGCTGCCTAAGGTTCCCTGGACAGTTGAACTCAGACCTCCGGAAGCTGGCCGTGAATCTCATTCCATTTCCACGTCTTCACTTCTTCATGGTCGGGTTTGCCCCTCTCACCTCTCGTGGATCTCAACAGTATATCTCCCTCACTGTGCCGGAGCTGACTCAGCAAATGTGGGATGCCAAGAACATGATGTGCGCCGCTGACCCTCGTCATGGACGTTACCTGACAGCCTCAGCTATGTTCAGGGGGAAGATGAGCACCAAAGAGGTGGACGAACAGATGATCAATGTGCAGAACAAGAACTCGTCCTACTTCGTTGAGTGGATCCCTCACAACGTGAAGTCAAGCGTATGCGATATTCCACCCAGGGGTTTGAAAATGGCATCTACCTTTGTTGGTAACTCTACATCAATCCAGGAGATGTTCAGGAGAGTGAGCGAGCAGTTCACAGCCATGTTCCGGCGCAAGGCCTTTTTGCATTGGTACACCGGTGAAGGCATGGACGAGATGGAGTTCACCGAAGCAGAGAGCAACATGAATGATTTGGTTGCGGAGTACCAGCAATACCAAGATGCAACCGTGGAGGATGAGGGCGAGTACGAGGAGGAAGGTGCCGAAGAGAATTACGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

50.45

Weight (kDa)

4.81

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 1e-64 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 263 - 384 5.6e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
Acc65I GGTACC 2 cut(s) 105, 218
AccB1I GGYRCC 4 cut(s) 105, 218, 293, 1325
AccB7I CCANNNNNTGG 2 cut(s) 60, 671
AccBSI CCGCTC 1 cut(s) 708
AccI GTMKAC 2 cut(s) 90, 153
AccII CGCG 2 cut(s) 111, 192
AccIII TCCGGA 1 cut(s) 756
AclWI GGATC 6 cut(s) 152, 213, 231, 844, 1033, 1046
AcoI YGGCCR 3 cut(s) 250, 283, 765
AcuI CTGAAG 1 cut(s) 625
AcyI GRCGYC 3 cut(s) 87, 117, 294
AdeI CACNNNGTG 1 cut(s) 1051
AfaI GTAC 7 cut(s) 107, 182, 198, 220, 1199, 1271, 1313
AfiI CCNNNNNNNGG 9 cut(s) 60, 102, 177, 671, 763, 926, 1082, 1173, 1180
AgeI ACCGGT 1 cut(s) 1202
AgsI TTSAA 3 cut(s) 398, 745, 1090
AjiI CACGTC 1 cut(s) 791
AjnI CCWGG 4 cut(s) 21, 733, 1079, 1128
AleI CACNNNNGTG 1 cut(s) 542
AluBI AGCT 7 cut(s) 136, 580, 655, 669, 763, 871, 950
AluI AGCT 7 cut(s) 136, 580, 655, 669, 763, 871, 950
Alw21I GWGCWC 3 cut(s) 84, 576, 974
Alw26I GTCTC 2 cut(s) 108, 535
Alw44I GTGCAC 1 cut(s) 572
AlwI GGATC 6 cut(s) 152, 213, 231, 844, 1033, 1046
Ama87I CYCGRG 1 cut(s) 137
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 8 cut(s) 28, 165, 241, 250, 283, 375, 765, 1182
ApaLI GTGCAC 1 cut(s) 572
ApeKI GCWGC 2 cut(s) 496, 720
AsiGI ACCGGT 1 cut(s) 1202
Asp700I GAANNNNTTC 1 cut(s) 436
Asp718I GGTACC 2 cut(s) 105, 218
AspLEI GCGC 4 cut(s) 194, 296, 911, 1179
AspS9I GGNCC 2 cut(s) 242, 645
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 5 cut(s) 513, 686, 817, 1217, 1222
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 645
AxyI CCTNAGG 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 576
BaeI ACNNNNGTAYC 2 cut(s) 1189, 1222
BamHI GGATCC 1 cut(s) 1038
BanI GGYRCC 4 cut(s) 105, 218, 293, 1325
BanII GRGCYC 1 cut(s) 216
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 2 cut(s) 271, 785
Bbv12I GWGCWC 3 cut(s) 84, 576, 974
BbvCI CCTCAGC 1 cut(s) 946
BbvI GCAGC 2 cut(s) 483, 707
BccI CCATC 1 cut(s) 1216
BceAI ACGGC 3 cut(s) 100, 179, 752
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 4 cut(s) 23, 735, 1081, 1130
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 2 cut(s) 108, 535
BfoI RGCGCY 1 cut(s) 297
BglI GCCNNNNNGGC 3 cut(s) 36, 249, 291
BglII AGATCT 1 cut(s) 255
BisI GCNGC 4 cut(s) 37, 497, 721, 912
BlpI GCTNAGC 1 cut(s) 656
BlsI GCNGC 4 cut(s) 38, 498, 722, 913
Bme1390I CCNGG 5 cut(s) 23, 216, 735, 1081, 1130
Bme18I GGWCC 1 cut(s) 645
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 791
BmgT120I GGNCC 2 cut(s) 242, 645
BmrFI CCNGG 5 cut(s) 23, 216, 735, 1081, 1130
BmsI GCATC 5 cut(s) 240, 483, 880, 1106, 1276
BoxI GACNNNNGTC 1 cut(s) 921
BpiI GAAGAC 2 cut(s) 271, 785
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 656
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 216
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 3 cut(s) 87, 117, 294
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 8 cut(s) 22, 96, 109, 222, 733, 1079, 1080, 1293
BsaWI WCCGGW 2 cut(s) 756, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 9 cut(s) 60, 102, 177, 671, 763, 926, 1082, 1173, 1180
Bse118I RCCGGY 3 cut(s) 119, 296, 1202
Bse1I ACTGG 2 cut(s) 286, 439
Bse21I CCTNAGG 1 cut(s) 725
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 4 cut(s) 23, 735, 1081, 1130
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 8 cut(s) 22, 96, 109, 222, 733, 1079, 1080, 1293
BseGI GGATG 5 cut(s) 18, 231, 498, 895, 1306
BseLI CCNNNNNNNGG 9 cut(s) 60, 102, 177, 671, 763, 926, 1082, 1173, 1180
BseMII CTCAG 6 cut(s) 327, 546, 670, 762, 890, 960
BseNI ACTGG 2 cut(s) 286, 439
BseRI GAGGAG 2 cut(s) 441, 1331
BseSI GKGCMC 1 cut(s) 576
BseXI GCAGC 2 cut(s) 483, 707
BsgI GTGCAG 1 cut(s) 1025
Bsh1236I CGCG 2 cut(s) 111, 192
BshFI GGCC 8 cut(s) 30, 167, 243, 252, 285, 377, 767, 1184
BshNI GGYRCC 4 cut(s) 105, 218, 293, 1325
BshTI ACCGGT 1 cut(s) 1202
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 3 cut(s) 84, 576, 974
BsiHKCI CYCGRG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 9 cut(s) 60, 102, 177, 671, 763, 926, 1082, 1173, 1180
BsmAI GTCTC 2 cut(s) 108, 535
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 8 cut(s) 30, 167, 243, 252, 285, 377, 767, 1184
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 4 cut(s) 84, 216, 576, 974
Bsp13I TCCGGA 1 cut(s) 756
Bsp1720I GCTNAGC 1 cut(s) 656
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 8 cut(s) 30, 167, 243, 252, 285, 377, 767, 1184
BspCNI CTCAG 6 cut(s) 328, 545, 669, 761, 889, 959
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 756
BspFNI CGCG 2 cut(s) 111, 192
BspPI GGATC 6 cut(s) 152, 213, 231, 844, 1033, 1046
BspT107I GGYRCC 4 cut(s) 105, 218, 293, 1325
BspTNI GGTCTC 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 708
BsrFI RCCGGY 3 cut(s) 119, 296, 1202
BsrI ACTGG 2 cut(s) 286, 439
BssAI RCCGGY 3 cut(s) 119, 296, 1202
BssECI CCNNGG 8 cut(s) 22, 96, 109, 222, 733, 1079, 1080, 1293
BssNI GRCGYC 3 cut(s) 87, 117, 294
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 4 cut(s) 23, 735, 1081, 1130
Bst4CI ACNGT 5 cut(s) 541, 741, 846, 862, 1294
Bst6I CTCTTC 1 cut(s) 1326
BstACI GRCGYC 3 cut(s) 87, 117, 294
BstC8I GCNNGC 4 cut(s) 121, 298, 765, 1154
BstDEI CTNAG 7 cut(s) 336, 532, 656, 725, 748, 876, 946
BstDSI CCRYGG 4 cut(s) 96, 109, 222, 1293
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 5 cut(s) 18, 231, 498, 895, 1306
BstFNI CGCG 2 cut(s) 111, 192
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 4 cut(s) 194, 296, 911, 1179
BstMAI GTCTC 2 cut(s) 108, 535
BstMWI GCNNNNNNNGC 5 cut(s) 36, 249, 291, 666, 877
BstNI CCWGG 4 cut(s) 23, 735, 1081, 1130
BstNSI RCATGY 1 cut(s) 720
BstPAI GACNNNNGTC 1 cut(s) 921
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 5 cut(s) 21, 214, 733, 1079, 1128
BstSLI GKGCMC 1 cut(s) 576
BstUI CGCG 2 cut(s) 111, 192
BstV1I GCAGC 2 cut(s) 483, 707
BstV2I GAAGAC 2 cut(s) 271, 785
BstX2I RGATCY 4 cut(s) 205, 255, 836, 1038
BstXI CCANNNNNNTGG 2 cut(s) 422, 982
BstYI RGATCY 4 cut(s) 205, 255, 836, 1038
Bsu15I ATCGAT 1 cut(s) 345
Bsu36I CCTNAGG 1 cut(s) 725
BsuRI GGCC 8 cut(s) 30, 167, 243, 252, 285, 377, 767, 1184
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 4 cut(s) 96, 109, 222, 1293
BtrI CACGTC 1 cut(s) 791
BtsCI GGATG 5 cut(s) 18, 231, 498, 895, 1306
BtsIMutI CAGTG 1 cut(s) 858
Cac8I GCNNGC 4 cut(s) 121, 298, 765, 1154
CfoI GCGC 4 cut(s) 194, 296, 911, 1179
Cfr10I RCCGGY 3 cut(s) 119, 296, 1202
Cfr13I GGNCC 2 cut(s) 242, 645
Cfr42I CCGCGG 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 2 cut(s) 76, 125
Csp6I GTAC 7 cut(s) 106, 181, 197, 219, 1198, 1270, 1312
CspAI ACCGGT 1 cut(s) 1202
CviQI GTAC 7 cut(s) 106, 181, 197, 219, 1198, 1270, 1312
DdeI CTNAG 7 cut(s) 336, 532, 656, 725, 748, 876, 946
DinI GGCGCC 1 cut(s) 295
DraIII CACNNNGTG 1 cut(s) 1051
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 3 cut(s) 250, 283, 765
Eam1104I CTCTTC 1 cut(s) 1326
EarI CTCTTC 1 cut(s) 1326
EciI GGCGGA 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 222
Eco147I AGGCCT 2 cut(s) 167, 1184
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 625
Eco81I CCTNAGG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 674
EcoO109I RGGNCCY 1 cut(s) 645
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 4 cut(s) 21, 733, 1079, 1128
EcoRV GATATC 1 cut(s) 693
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 216
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 108
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 2 cut(s) 90, 153
Fnu4HI GCNGC 4 cut(s) 37, 497, 721, 912
FokI GGATG 5 cut(s) 5, 218, 505, 902, 1313
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 4 cut(s) 37, 497, 721, 912
GlaI GCGC 4 cut(s) 193, 295, 910, 1178
GluI GCNGC 4 cut(s) 37, 497, 721, 912
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 8 cut(s) 30, 167, 243, 252, 285, 377, 767, 1184
HgaI GACGC 2 cut(s) 76, 125
HhaI GCGC 4 cut(s) 194, 296, 911, 1179
Hin1I GRCGYC 3 cut(s) 87, 117, 294
Hin6I GCGC 4 cut(s) 192, 294, 909, 1177
HinP1I GCGC 4 cut(s) 192, 294, 909, 1177
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 4 cut(s) 10, 347, 772, 874
HphI GGTGA 5 cut(s) 513, 686, 817, 1217, 1222
Hpy166II GTNNAC 7 cut(s) 91, 154, 574, 985, 1161, 1200, 1230
Hpy188I TCNGA 4 cut(s) 127, 236, 535, 751
Hpy188III TCNNGA 8 cut(s) 139, 209, 356, 441, 611, 643, 757, 1141
Hpy8I GTNNAC 7 cut(s) 91, 154, 574, 985, 1161, 1200, 1230
Hpy99I CGWCG 2 cut(s) 80, 92
HpyAV CCTTC 8 cut(s) 96, 256, 323, 404, 464, 528, 1202, 1316
HpyCH4III ACNGT 5 cut(s) 541, 741, 846, 862, 1294
HpyCH4IV ACGT 6 cut(s) 150, 183, 503, 790, 931, 1050
HpyCH4V TGCA 6 cut(s) 16, 574, 603, 1006, 1192, 1289
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 249, 291, 666, 877
HpyF3I CTNAG 7 cut(s) 336, 532, 656, 725, 748, 876, 946
HpySE526I ACGT 6 cut(s) 150, 183, 503, 790, 931, 1050
Hsp92I GRCGYC 3 cut(s) 87, 117, 294
HspAI GCGC 4 cut(s) 192, 294, 909, 1177
KasI GGCGCC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 756
KpnI GGTACC 2 cut(s) 109, 222
KroI GCCGGC 2 cut(s) 119, 296
KroNI GCCGGC 2 cut(s) 121, 298
Ksp22I TGATCA 1 cut(s) 996
KspI CCGCGG 1 cut(s) 112
LmnI GCTCC 3 cut(s) 56, 128, 868
Lsp1109I GCAGC 2 cut(s) 483, 707
LweI GCATC 5 cut(s) 240, 483, 880, 1106, 1276
MaeII ACGT 6 cut(s) 150, 183, 503, 790, 931, 1050
MaeIII GTNAC 4 cut(s) 674, 712, 932, 1112
MbiI CCGCTC 1 cut(s) 708
MboII GAAGA 6 cut(s) 250, 271, 785, 793, 976, 1343
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 4 cut(s) 205, 255, 836, 1038
MhlI GDGCHC 4 cut(s) 84, 216, 576, 974
MluCI AATT 3 cut(s) 304, 382, 1336
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 1 cut(s) 868
Mph1103I ATGCAT 1 cut(s) 605
MroI TCCGGA 1 cut(s) 756
MroNI GCCGGC 2 cut(s) 119, 296
MroXI GAANNNNTTC 1 cut(s) 436
MslI CAYNNNNRTG 2 cut(s) 542, 1166
MspA1I CMGCKG 3 cut(s) 111, 580, 914
MspR9I CCNGG 5 cut(s) 23, 216, 735, 1081, 1130
MunI CAATTG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 605
MvaI CCWGG 4 cut(s) 23, 735, 1081, 1130
MvnI CGCG 2 cut(s) 111, 192
MwoI GCNNNNNNNGC 5 cut(s) 36, 249, 291, 666, 877
NaeI GCCGGC 2 cut(s) 121, 298
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 222
NgoMIV GCCGGC 2 cut(s) 119, 296
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 674
NsiI ATGCAT 1 cut(s) 605
NspI RCATGY 1 cut(s) 720
OliI CACNNNNGTG 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 137
PasI CCCWGGG 1 cut(s) 1080
PceI AGGCCT 2 cut(s) 167, 1184
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 123, 928
PctI GAATGC 1 cut(s) 605
PdiI GCCGGC 2 cut(s) 121, 298
PdmI GAANNNNTTC 1 cut(s) 436
PfeI GAWTC 3 cut(s) 10, 347, 772
PflMI CCANNNNNTGG 2 cut(s) 60, 671
PfoI TCCNGGA 1 cut(s) 1128
PinAI ACCGGT 1 cut(s) 1202
PkrI GCNGC 4 cut(s) 38, 498, 722, 913
PleI GAGTC 1 cut(s) 868
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 868
PpuMI RGGWCCY 1 cut(s) 645
PshAI GACNNNNGTC 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 645
Psp6I CCWGG 4 cut(s) 21, 733, 1079, 1128
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 4 cut(s) 21, 733, 1079, 1128
PspPI GGNCC 2 cut(s) 242, 645
PspPPI RGGWCCY 1 cut(s) 645
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
PsuI RGATCY 4 cut(s) 205, 255, 836, 1038
PvuII CAGCTG 1 cut(s) 580
RsaI GTAC 7 cut(s) 107, 182, 198, 220, 1199, 1271, 1313
RsaNI GTAC 7 cut(s) 106, 181, 197, 219, 1198, 1270, 1312
RseI CAYNNNNRTG 2 cut(s) 542, 1166
SacII CCGCGG 1 cut(s) 112
SalI GTCGAC 1 cut(s) 89
SatI GCNGC 4 cut(s) 37, 497, 721, 912
Sau96I GGNCC 2 cut(s) 242, 645
SchI GAGTC 1 cut(s) 868
ScrFI CCNGG 5 cut(s) 23, 216, 735, 1081, 1130
SduI GDGCHC 4 cut(s) 84, 216, 576, 974
SfaNI GCATC 5 cut(s) 240, 483, 880, 1106, 1276
SfiI GGCCNNNNNGGCC 1 cut(s) 249
SfoI GGCGCC 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 137
Sfr303I CCGCGG 1 cut(s) 112
SgrAI CRCCGGYG 1 cut(s) 1202
SgrBI CCGCGG 1 cut(s) 112
SinI GGWCC 1 cut(s) 645
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 542, 1166
SmlI CTYRAG 2 cut(s) 137, 209
SmoI CTYRAG 2 cut(s) 137, 209
Sse9I AATT 3 cut(s) 304, 382, 1336
SseBI AGGCCT 2 cut(s) 167, 1184
SspDI GGCGCC 1 cut(s) 293
StuI AGGCCT 2 cut(s) 167, 1184
StyD4I CCNGG 5 cut(s) 21, 214, 733, 1079, 1128
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 5 cut(s) 541, 741, 846, 862, 1294
TaiI ACGT 6 cut(s) 153, 186, 506, 793, 934, 1053
TaqI TCGA 4 cut(s) 90, 138, 345, 357
TasI AATT 3 cut(s) 304, 382, 1336
TauI GCSGC 2 cut(s) 39, 914
TfiI GAWTC 3 cut(s) 10, 347, 772
TscAI CASTG 1 cut(s) 865
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 2 cut(s) 496, 720
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 3 cut(s) 612, 793, 1265
TspGWI ACGGA 1 cut(s) 340
TspRI CASTG 1 cut(s) 865
Van91I CCANNNNNTGG 2 cut(s) 60, 671
VneI GTGCAC 1 cut(s) 572
VpaK11BI GGWCC 1 cut(s) 645
XceI RCATGY 1 cut(s) 720
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 90, 153
XmnI GAANNNNTTC 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.