RchiOBHm_Chr7g0219301

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011653
Physical Location & Seq
Reverse (-)
37645721 .. 37646569
849 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19628

Sequence Viewer

Length: 402 bp
ATGGGGACTCTGTTGATTTTGAAAATCAGGGAGGAATACCCTGACAGGATGATGCTCACTTTCTCTGTGTTTCCTTCACCAAAAGTCTCCGATACGGTGGTTGAGCCCTATAGTGCTACCTTGTCTGTGCACCAGCTTGTTGAGAATGCGGATGAGTGTATGGTTCTTGATAACGAAGCTCTCTATGACATTTGCTTCCGCACACTCAAGCTCAGTACCCCAAGCTTTGGCGATTTGAACCATTTGATTTCTGCTACCATGTCTGGTGTGACTTGCTGTTTGAGGTTCCCTGGGCAACTCAACTCTGACCTCAGAAAGTTGGCTGCCAATCTCATTCCATTCCCCCATCTCCACTTTTTCATTCCATTCCTGGTGGGATGTTTTAATGCAGAAAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

133

Amino Acids

14.93

Weight (kDa)

5.44

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 2 - 65 8.7e-14 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 227
AciI CCGC 2 cut(s) 149, 199
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 1 cut(s) 227
AgsI TTSAA 2 cut(s) 22, 238
AjnI CCWGG 2 cut(s) 289, 369
AluBI AGCT 4 cut(s) 136, 179, 211, 225
AluI AGCT 4 cut(s) 136, 179, 211, 225
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 91
Alw44I GTGCAC 1 cut(s) 128
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 323
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
AsuHPI GGTGA 1 cut(s) 69
BaeGI GKGCMC 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 84, 117
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 310
BccI CCATC 1 cut(s) 354
BciT130I CCWGG 2 cut(s) 291, 371
BcoDI GTCTC 1 cut(s) 91
BfmI CTRYAG 1 cut(s) 109
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
Bme1390I CCNGG 2 cut(s) 291, 371
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 2 cut(s) 291, 371
BmsI GCATC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 191
BsaJI CCNNGG 2 cut(s) 289, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 227
BseBI CCWGG 2 cut(s) 291, 371
BseDI CCNNGG 2 cut(s) 289, 290
BseGI GGATG 3 cut(s) 54, 157, 383
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMII CTCAG 2 cut(s) 226, 325
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 132
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 91
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 108, 132
BspACI CCGC 2 cut(s) 149, 199
BspCNI CTCAG 2 cut(s) 225, 324
BspLI GGNNCC 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 289, 290
Bst2UI CCWGG 2 cut(s) 291, 371
Bst4CI ACNGT 1 cut(s) 97
BstDEI CTNAG 2 cut(s) 212, 311
BstF5I GGATG 3 cut(s) 54, 157, 383
BstMAI GTCTC 1 cut(s) 91
BstNI CCWGG 2 cut(s) 291, 371
BstSCI CCNGG 2 cut(s) 289, 369
BstSFI CTRYAG 1 cut(s) 109
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 310
BtsCI GGATG 3 cut(s) 54, 157, 383
Csp6I GTAC 1 cut(s) 216
CviAII CATG 1 cut(s) 259
CviJI RGCY 6 cut(s) 106, 136, 179, 211, 225, 323
CviKI_1 RGCY 6 cut(s) 106, 136, 179, 211, 225, 323
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 2 cut(s) 212, 311
Eco24I GRGCYC 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 289, 369
EcoT38I GRGCYC 1 cut(s) 108
FaeI CATG 1 cut(s) 262
FaiI YATR 4 cut(s) 111, 161, 186, 260
FaqI GGGAC 1 cut(s) 19
FatI CATG 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 3 cut(s) 61, 164, 390
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 324
GluI GCNGC 1 cut(s) 324
Hin1II CATG 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 1 cut(s) 7
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 3 cut(s) 91, 307, 314
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 2 cut(s) 130, 389
HpyF3I CTNAG 2 cut(s) 212, 311
Hsp92II CATG 1 cut(s) 262
LpnPI CCDG 9 cut(s) 13, 31, 54, 146, 249, 276, 303, 356, 383
Lsp1109I GCAGC 1 cut(s) 310
LweI GCATC 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 108, 132
MnlI CCTC 3 cut(s) 25, 276, 320
MseI TTAA 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 291, 371
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 2 cut(s) 291, 371
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 268
PasI CCCWGGG 1 cut(s) 290
PctI GAATGC 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 289, 369
PspGI CCWGG 2 cut(s) 289, 369
PspN4I GGNNCC 1 cut(s) 287
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 1 cut(s) 324
ScrFI CCNGG 2 cut(s) 291, 371
SduI GDGCHC 2 cut(s) 108, 132
SetI ASST 7 cut(s) 122, 138, 181, 213, 227, 287, 312
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SsiI CCGC 2 cut(s) 149, 199
StyD4I CCNGG 2 cut(s) 289, 369
TaaI ACNGT 1 cut(s) 97
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 227
VneI GTGCAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.