FvH4_2g38400

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011653
Physical Location & Seq
Forward (+)
27792831 .. 27796079
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38400.t2

Sequence Viewer

Length: 1350 bp
ATGAGAGAGATCCTCCACATTCAGGGCGGCCAATGCGGCAACCAAATCGGCTCCAAGTTCTGGGAGGTCATCTGCGACGAGCACGGCGTCGACCCCACCGGCCGTTACAAAGGCGACGGCTCCTCCGATCTCCAGCTCGAGAGGATCAACGTCTACTACAATGAGGCCTCCGGCGGAAGGTACGTCCCCCGCGCCGTCCTCATGGATCTCGAGCCCGGAACCATGGACAGCATCAGATCCGGCCCGTTCGGACAGATCTTCCGCCCCGATAACTTCGTCTTCGGCCAGTCCGGCGCCGGCAACAACTGGGCCAAAGGTCACTACACCGAAGGTGCTGAGCTCATCGATTCTGTTCTTGATGTTGTTCGCAAGGAGGCCGAGAATTGCGATTGCCTTCAAGGTTTCCAGGTGTGTCACTCACTTGGAGGAGGGACTGGTTCTGGCATGGGAACCCTTCTGATATCAAAGATTAGAGAGGAATATCCTGACAGGATGATGCTTACATTCTCTGTGTTCCCATCTCCAAAGGTCTCAGACACTGTTGTGGAACCATACAATGCCACTCTTTCAGTGCACCAGTTGGTAGAGAATGCTGATGAATGCATGGTTCTAGATAATGAAGCTCTTTACGACATTTGCTTCAGAACCCTGAAGCTCAGTACCCCAAGCTTTGGTGACCTCAACCATTTGATTTCTGCAACTATGAGTGGTGTTACTTGCTGCCTAAGGTTCCCGGGTCAGCTGAACTCAGATCTCCGGAAGCTGGCTGTCAATCTCATTCCATTCCCACGTCTCCACTTCTTCATGGTTGGGTTTGCCCCACTCACATCTCGCGGTTCCCAGCAGTACATCTCCCTCACTGTGCCAGAGCTGACTCAGCAAATGTGGGATGCCAAGAACATGATGTGCGCTGCTGATCCTCGCCATGGAAGGTACCTGACAGCCTCAGCCATGTTCAGGGGTAAGATGAGCACCAAAGAGGTGGACGAACAGATGATCAACGTGCAGAACAAGAACTCTTCCTACTTTGTCGAGTGGATTCCTCACAATGTGAAGTCCAGCGTGTGCGACATCCCTCCCCTGGGATTGAAAATGGCATCCACCTTTGTTGGTAACTCCACCTCAATCCAGGAGATGTTCAGGAGGGTCAGCGAGCAGTTCACAGCCATGTTCAGGCGCAAGGCCTTTTTGCATTGGTATACCGGAGAAGGTATGGACGAGATGGAGTTCACTGAAGCTGAGAGTAACATGAATGATTTGGTTGCCGAGTACCAGCAATACCAGGATGCTACGGTGGAGGATGAAGGTGAGTATGAGGAGGATGGTGCTGAAGGTGAGTATGAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04145 ko04540 ko05130 map04145 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

450

Amino Acids

50.33

Weight (kDa)

4.76

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 25 - 105 2.9e-25 Tubulin/FtsZ family, GTPase domain
Tubulin_C PF03953 155 - 276 3.3e-39 Tubulin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011653)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20010 AT1G75780
fragaria_vesca FvH4_2g38400 FvH4_2g38400
malus_domestica MD08G1012800.v1.1 MD15G1011800.v1.1
prunus_persica Prupe.1G364800_v2.0.a1
pyrus_communis pycom08g01090 pycom15g01040
rosa_chinensis RchiOBHm_Chr6g0302901 RchiOBHm_Chr7g0219301
rosa_laevigata RLG00000011125
rosa_multiflora Rmu_ssc0000460.1_g000016
rosa_rugosa Rorug06G0318000
rosa_samantha Rh6AG431600 Rh6BG471000 Rh6CG444000 Rh6DG430800
rosa_wichuraiana Rw6G037410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 933
AccB1I GGYRCC 2 cut(s) 293, 933
AccB7I CCANNNNNTGG 2 cut(s) 60, 671
AccI GTMKAC 3 cut(s) 90, 153, 1199
AccII CGCG 2 cut(s) 192, 834
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 6 cut(s) 27, 36, 174, 190, 262, 834
AclWI GGATC 5 cut(s) 4, 152, 213, 231, 911
AcoI YGGCCR 3 cut(s) 28, 100, 283
AcuI CTGAAG 4 cut(s) 625, 671, 1254, 1350
AcyI GRCGYC 2 cut(s) 87, 294
AdeI CACNNNGTG 1 cut(s) 1051
AfaI GTAC 5 cut(s) 182, 661, 848, 935, 1271
AgsI TTSAA 2 cut(s) 398, 1090
AjiI CACGTC 1 cut(s) 791
AjnI CCWGG 4 cut(s) 405, 1080, 1128, 1281
AleI CACNNNNGTG 1 cut(s) 542
AluBI AGCT 9 cut(s) 136, 340, 623, 655, 669, 742, 763, 871, 1238
AluI AGCT 9 cut(s) 136, 340, 623, 655, 669, 742, 763, 871, 1238
Alw21I GWGCWC 4 cut(s) 84, 342, 576, 974
Alw26I GTCTC 2 cut(s) 535, 797
Alw44I GTGCAC 1 cut(s) 572
AlwI GGATC 5 cut(s) 4, 152, 213, 231, 911
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 3 cut(s) 137, 209, 733
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 8 cut(s) 28, 100, 165, 241, 283, 309, 375, 1182
ApaLI GTGCAC 1 cut(s) 572
ApeKI GCWGC 2 cut(s) 720, 911
Asp718I GGTACC 1 cut(s) 933
AspLEI GCGC 4 cut(s) 194, 296, 911, 1179
AspS9I GGNCC 2 cut(s) 242, 309
AsuC2I CCSGG 3 cut(s) 216, 734, 735
AsuHPI GGTGA 3 cut(s) 686, 1319, 1346
AvaI CYCGRG 3 cut(s) 137, 209, 733
AxyI CCTNAGG 1 cut(s) 725
BaeGI GKGCMC 1 cut(s) 576
BanI GGYRCC 2 cut(s) 293, 933
BanII GRGCYC 2 cut(s) 216, 342
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 4 cut(s) 84, 342, 576, 974
BbvCI CCTCAGC 1 cut(s) 946
BbvI GCAGC 2 cut(s) 707, 898
BccI CCATC 3 cut(s) 526, 1216, 1316
BceAI ACGGC 4 cut(s) 87, 100, 133, 179
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 4 cut(s) 407, 1082, 1130, 1283
BclI TGATCA 1 cut(s) 996
BcnI CCSGG 3 cut(s) 216, 734, 735
BcoDI GTCTC 2 cut(s) 535, 797
BfaI CTAG 1 cut(s) 611
BfoI RGCGCY 1 cut(s) 297
BglI GCCNNNNNGGC 2 cut(s) 36, 291
BglII AGATCT 2 cut(s) 255, 751
BisI GCNGC 4 cut(s) 28, 37, 721, 912
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 4 cut(s) 29, 38, 722, 913
Bme1390I CCNGG 7 cut(s) 216, 407, 734, 735, 1082, 1130, 1283
BmeT110I CYCGRG 3 cut(s) 137, 209, 733
BmgBI CACGTC 1 cut(s) 791
BmgT120I GGNCC 2 cut(s) 242, 309
BmiI GGNNCC 9 cut(s) 52, 121, 220, 295, 451, 549, 731, 838, 935
BmrFI CCNGG 7 cut(s) 216, 407, 734, 735, 1082, 1130, 1283
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 5 cut(s) 240, 486, 880, 1106, 1276
BmuI ACTGGG 1 cut(s) 316
BpiI GAAGAC 1 cut(s) 271
BplI GAGNNNNNCTC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 946
Bpu1102I GCTNAGC 1 cut(s) 336
BpuMI CCSGG 3 cut(s) 216, 734, 735
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 87, 294
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 5 cut(s) 222, 733, 925, 1080, 1081
BsaWI WCCGGW 2 cut(s) 756, 1202
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bse118I RCCGGY 2 cut(s) 98, 296
Bse1I ACTGG 4 cut(s) 286, 311, 439, 577
Bse21I CCTNAGG 1 cut(s) 725
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 4 cut(s) 407, 1082, 1130, 1283
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 222, 733, 925, 1080, 1081
BseGI GGATG 7 cut(s) 498, 895, 1071, 1097, 1291, 1306, 1327
BseMII CTCAG 7 cut(s) 327, 546, 670, 762, 890, 960, 1230
BseNI ACTGG 4 cut(s) 286, 311, 439, 577
BseRI GAGGAG 3 cut(s) 112, 441, 1331
BseSI GKGCMC 1 cut(s) 576
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 2 cut(s) 707, 898
BseYI CCCAGC 1 cut(s) 840
BsgI GTGCAG 1 cut(s) 1025
Bsh1236I CGCG 2 cut(s) 192, 834
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 8 cut(s) 30, 102, 167, 243, 285, 311, 377, 1184
BshNI GGYRCC 2 cut(s) 293, 933
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 4 cut(s) 84, 342, 576, 974
BsiHKCI CYCGRG 3 cut(s) 137, 209, 733
BsiSI CCGG 9 cut(s) 99, 171, 216, 240, 291, 297, 734, 757, 1203
BslFI GGGAC 2 cut(s) 170, 445
BsmAI GTCTC 2 cut(s) 535, 797
BsmBI CGTCTC 1 cut(s) 797
BsmFI GGGAC 2 cut(s) 170, 445
BsmI GAATGC 2 cut(s) 595, 605
BsnI GGCC 8 cut(s) 30, 102, 167, 243, 285, 311, 377, 1184
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 3 cut(s) 137, 209, 733
Bsp1286I GDGCHC 5 cut(s) 84, 216, 342, 576, 974
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
Bsp1720I GCTNAGC 1 cut(s) 336
Bsp19I CCATGG 2 cut(s) 222, 925
BspACI CCGC 6 cut(s) 27, 36, 174, 190, 262, 834
BspANI GGCC 8 cut(s) 30, 102, 167, 243, 285, 311, 377, 1184
BspCNI CTCAG 7 cut(s) 328, 545, 669, 761, 889, 959, 1231
BspDI ATCGAT 1 cut(s) 345
BspEI TCCGGA 1 cut(s) 756
BspFNI CGCG 2 cut(s) 192, 834
BspLI GGNNCC 9 cut(s) 52, 121, 220, 295, 451, 549, 731, 838, 935
BspPI GGATC 5 cut(s) 4, 152, 213, 231, 911
BspT107I GGYRCC 2 cut(s) 293, 933
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 2 cut(s) 98, 296
BsrI ACTGG 4 cut(s) 286, 311, 439, 577
BssAI RCCGGY 2 cut(s) 98, 296
BssECI CCNNGG 5 cut(s) 222, 733, 925, 1080, 1081
BssMI GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
BssNAI GTATAC 1 cut(s) 1200
BssNI GRCGYC 2 cut(s) 87, 294
BssT1I CCWWGG 2 cut(s) 222, 925
Bst1107I GTATAC 1 cut(s) 1200
Bst2UI CCWGG 4 cut(s) 407, 1082, 1130, 1283
Bst4CI ACNGT 3 cut(s) 541, 862, 1294
Bst6I CTCTTC 1 cut(s) 1024
BstACI GRCGYC 2 cut(s) 87, 294
BstC8I GCNNGC 3 cut(s) 298, 765, 1154
BstDEI CTNAG 8 cut(s) 336, 532, 656, 725, 748, 876, 946, 1239
BstDSI CCRYGG 2 cut(s) 222, 925
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 7 cut(s) 498, 895, 1071, 1097, 1291, 1306, 1327
BstFNI CGCG 2 cut(s) 192, 834
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 4 cut(s) 194, 296, 911, 1179
BstKTI GATC 9 cut(s) 12, 130, 147, 208, 239, 258, 754, 919, 999
BstMAI GTCTC 2 cut(s) 535, 797
BstMBI GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 4 cut(s) 33, 36, 291, 877
BstNI CCWGG 4 cut(s) 407, 1082, 1130, 1283
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 7 cut(s) 214, 405, 732, 733, 1080, 1128, 1281
BstSLI GKGCMC 1 cut(s) 576
BstUI CGCG 2 cut(s) 192, 834
BstV1I GCAGC 2 cut(s) 707, 898
BstV2I GAAGAC 1 cut(s) 271
BstX2I RGATCY 5 cut(s) 9, 205, 236, 255, 751
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 5 cut(s) 9, 205, 236, 255, 751
BstZ17I GTATAC 1 cut(s) 1200
BstZI CGGCCG 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 345
Bsu36I CCTNAGG 1 cut(s) 725
BsuRI GGCC 8 cut(s) 30, 102, 167, 243, 285, 311, 377, 1184
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 2 cut(s) 222, 925
BtrI CACGTC 1 cut(s) 791
BtsCI GGATG 7 cut(s) 498, 895, 1071, 1097, 1291, 1306, 1327
BtsIMutI CAGTG 4 cut(s) 537, 576, 858, 1230
Cac8I GCNNGC 3 cut(s) 298, 765, 1154
CaiI CAGNNNCTG 1 cut(s) 539
CfoI GCGC 4 cut(s) 194, 296, 911, 1179
Cfr10I RCCGGY 2 cut(s) 98, 296
Cfr13I GGNCC 2 cut(s) 242, 309
Cfr9I CCCGGG 1 cut(s) 733
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 1 cut(s) 76
Csp6I GTAC 5 cut(s) 181, 660, 847, 934, 1270
CspCI CAANNNNNGTGG 2 cut(s) 1090, 1125
CviQI GTAC 5 cut(s) 181, 660, 847, 934, 1270
DdeI CTNAG 8 cut(s) 336, 532, 656, 725, 748, 876, 946, 1239
DinI GGCGCC 1 cut(s) 295
DpnI GATC 9 cut(s) 11, 129, 146, 207, 238, 257, 753, 918, 998
DpnII GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
DraIII CACNNNGTG 1 cut(s) 1051
EaeI YGGCCR 3 cut(s) 28, 100, 283
EagI CGGCCG 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 1024
EarI CTCTTC 1 cut(s) 1024
EciI GGCGGA 2 cut(s) 189, 251
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 222, 925
Eco147I AGGCCT 2 cut(s) 167, 1184
Eco24I GRGCYC 2 cut(s) 216, 342
Eco31I GGTCTC 1 cut(s) 535
Eco32I GATATC 1 cut(s) 462
Eco52I CGGCCG 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 4 cut(s) 625, 671, 1254, 1350
Eco81I CCTNAGG 1 cut(s) 725
Eco88I CYCGRG 3 cut(s) 137, 209, 733
Eco91I GGTNACC 1 cut(s) 674
EcoICRI GAGCTC 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 674
EcoRII CCWGG 4 cut(s) 405, 1080, 1128, 1281
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 2 cut(s) 222, 925
EcoT22I ATGCAT 1 cut(s) 605
EcoT38I GRGCYC 2 cut(s) 216, 342
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 2 cut(s) 222, 925
Esp3I CGTCTC 1 cut(s) 797
FaqI GGGAC 2 cut(s) 170, 445
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 996
FblI GTMKAC 3 cut(s) 90, 153, 1199
Fnu4HI GCNGC 4 cut(s) 28, 37, 721, 912
FokI GGATG 7 cut(s) 505, 902, 1058, 1084, 1298, 1313, 1334
FriOI GRGCYC 2 cut(s) 216, 342
Fsp4HI GCNGC 4 cut(s) 28, 37, 721, 912
FspBI CTAG 1 cut(s) 611
GlaI GCGC 4 cut(s) 193, 295, 910, 1178
GluI GCNGC 4 cut(s) 28, 37, 721, 912
GsaI CCCAGC 1 cut(s) 844
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 8 cut(s) 30, 102, 167, 243, 285, 311, 377, 1184
HapII CCGG 9 cut(s) 99, 171, 216, 240, 291, 297, 734, 757, 1203
HgaI GACGC 1 cut(s) 76
HhaI GCGC 4 cut(s) 194, 296, 911, 1179
Hin1I GRCGYC 2 cut(s) 87, 294
Hin6I GCGC 4 cut(s) 192, 294, 909, 1177
HinP1I GCGC 4 cut(s) 192, 294, 909, 1177
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 3 cut(s) 347, 874, 1039
HpaII CCGG 9 cut(s) 99, 171, 216, 240, 291, 297, 734, 757, 1203
HphI GGTGA 3 cut(s) 686, 1319, 1346
Hpy166II GTNNAC 7 cut(s) 91, 154, 574, 985, 1161, 1200, 1230
Hpy188I TCNGA 7 cut(s) 127, 236, 251, 459, 535, 644, 751
Hpy188III TCNNGA 7 cut(s) 139, 209, 356, 485, 611, 757, 1141
Hpy8I GTNNAC 7 cut(s) 91, 154, 574, 985, 1161, 1200, 1230
Hpy99I CGWCG 3 cut(s) 80, 92, 119
HpyAV CCTTC 8 cut(s) 171, 323, 404, 464, 924, 1202, 1298, 1325
HpyCH4III ACNGT 3 cut(s) 541, 862, 1294
HpyCH4IV ACGT 4 cut(s) 150, 183, 790, 1002
HpyCH4V TGCA 5 cut(s) 574, 603, 698, 1006, 1192
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 36, 291, 877
HpyF3I CTNAG 8 cut(s) 336, 532, 656, 725, 748, 876, 946, 1239
HpySE526I ACGT 4 cut(s) 150, 183, 790, 1002
Hsp92I GRCGYC 2 cut(s) 87, 294
HspAI GCGC 4 cut(s) 192, 294, 909, 1177
KasI GGCGCC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 756
KpnI GGTACC 1 cut(s) 937
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 996
Kzo9I GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
LmnI GCTCC 2 cut(s) 56, 125
Lsp1109I GCAGC 2 cut(s) 707, 898
LweI GCATC 5 cut(s) 240, 486, 880, 1106, 1276
MaeI CTAG 1 cut(s) 611
MaeII ACGT 4 cut(s) 150, 183, 790, 1002
MaeIII GTNAC 7 cut(s) 104, 317, 413, 674, 712, 1112, 1244
MalI GATC 9 cut(s) 11, 129, 146, 207, 238, 257, 753, 918, 998
MboI GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
MboII GAAGA 4 cut(s) 250, 271, 793, 1011
MflI RGATCY 5 cut(s) 9, 205, 236, 255, 751
MhlI GDGCHC 5 cut(s) 84, 216, 342, 576, 974
MluCI AATT 1 cut(s) 382
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 1 cut(s) 868
Mph1103I ATGCAT 1 cut(s) 605
MroI TCCGGA 1 cut(s) 756
MroNI GCCGGC 1 cut(s) 296
MslI CAYNNNNRTG 2 cut(s) 542, 1166
MspA1I CMGCKG 1 cut(s) 742
MspI CCGG 9 cut(s) 99, 171, 216, 240, 291, 297, 734, 757, 1203
MspR9I CCNGG 7 cut(s) 216, 407, 734, 735, 1082, 1130, 1283
Mva1269I GAATGC 2 cut(s) 595, 605
MvaI CCWGG 4 cut(s) 407, 1082, 1130, 1283
MvnI CGCG 2 cut(s) 192, 834
MwoI GCNNNNNNNGC 4 cut(s) 33, 36, 291, 877
NaeI GCCGGC 1 cut(s) 298
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 3 cut(s) 216, 734, 735
NcoI CCATGG 2 cut(s) 222, 925
NdeII GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
NgoMIV GCCGGC 1 cut(s) 296
NlaIV GGNNCC 9 cut(s) 52, 121, 220, 295, 451, 549, 731, 838, 935
NmeAIII GCCGAG 2 cut(s) 403, 1291
NmuCI GTSAC 3 cut(s) 317, 413, 674
NsiI ATGCAT 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 542
PaeR7I CTCGAG 2 cut(s) 137, 209
PasI CCCWGGG 1 cut(s) 1081
PceI AGGCCT 2 cut(s) 167, 1184
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 123
PctI GAATGC 2 cut(s) 595, 605
PdiI GCCGGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 347, 1039
PflMI CCANNNNNTGG 2 cut(s) 60, 671
PfoI TCCNGGA 1 cut(s) 1128
PkrI GCNGC 4 cut(s) 29, 38, 722, 913
PleI GAGTC 1 cut(s) 868
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 868
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 4 cut(s) 405, 1080, 1128, 1281
PspEI GGTNACC 1 cut(s) 674
PspFI CCCAGC 1 cut(s) 840
PspGI CCWGG 4 cut(s) 405, 1080, 1128, 1281
PspN4I GGNNCC 9 cut(s) 52, 121, 220, 295, 451, 549, 731, 838, 935
PspPI GGNCC 2 cut(s) 242, 309
PsrI GAACNNNNNNTAC 2 cut(s) 1007, 1039
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 5 cut(s) 9, 205, 236, 255, 751
PvuII CAGCTG 1 cut(s) 742
RsaI GTAC 5 cut(s) 182, 661, 848, 935, 1271
RsaNI GTAC 5 cut(s) 181, 660, 847, 934, 1270
RseI CAYNNNNRTG 2 cut(s) 542, 1166
SacI GAGCTC 1 cut(s) 342
SalI GTCGAC 1 cut(s) 89
SatI GCNGC 4 cut(s) 28, 37, 721, 912
Sau3AI GATC 9 cut(s) 9, 127, 144, 205, 236, 255, 751, 916, 996
Sau96I GGNCC 2 cut(s) 242, 309
SchI GAGTC 1 cut(s) 868
ScrFI CCNGG 7 cut(s) 216, 407, 734, 735, 1082, 1130, 1283
SduI GDGCHC 5 cut(s) 84, 216, 342, 576, 974
SfaNI GCATC 5 cut(s) 240, 486, 880, 1106, 1276
SfoI GGCGCC 1 cut(s) 295
Sfr274I CTCGAG 2 cut(s) 137, 209
SlaI CTCGAG 2 cut(s) 137, 209
SmaI CCCGGG 1 cut(s) 735
SmiMI CAYNNNNRTG 2 cut(s) 542, 1166
SmlI CTYRAG 2 cut(s) 137, 209
SmoI CTYRAG 2 cut(s) 137, 209
Sse9I AATT 1 cut(s) 382
SseBI AGGCCT 2 cut(s) 167, 1184
SsiI CCGC 6 cut(s) 27, 36, 174, 190, 262, 834
SspDI GGCGCC 1 cut(s) 293
SspMI CTAG 1 cut(s) 611
SstI GAGCTC 1 cut(s) 342
StuI AGGCCT 2 cut(s) 167, 1184
StyD4I CCNGG 7 cut(s) 214, 405, 732, 733, 1080, 1128, 1281
StyI CCWWGG 2 cut(s) 222, 925
TaaI ACNGT 3 cut(s) 541, 862, 1294
TaiI ACGT 4 cut(s) 153, 186, 793, 1005
TaqI TCGA 5 cut(s) 90, 138, 210, 345, 1032
TasI AATT 1 cut(s) 382
TatI WGTACW 1 cut(s) 846
TauI GCSGC 2 cut(s) 30, 39
TfiI GAWTC 2 cut(s) 347, 1039
TscAI CASTG 4 cut(s) 544, 576, 865, 1237
TseFI GTSAC 3 cut(s) 317, 413, 674
TseI GCWGC 2 cut(s) 720, 911
Tsp45I GTSAC 3 cut(s) 317, 413, 674
TspDTI ATGAA 5 cut(s) 612, 633, 793, 1265, 1317
TspMI CCCGGG 1 cut(s) 733
TspRI CASTG 4 cut(s) 544, 576, 865, 1237
Van91I CCANNNNNTGG 2 cut(s) 60, 671
VneI GTGCAC 1 cut(s) 572
XbaI TCTAGA 1 cut(s) 610
XhoI CTCGAG 2 cut(s) 137, 209
XmaI CCCGGG 1 cut(s) 733
XmiI GTMKAC 3 cut(s) 90, 153, 1199
XspI CTAG 1 cut(s) 611
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.