Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014352

Physical Location & Seq

Genomic Coordinates Forward (+)

7029629 .. 7031535

Gene Structure 1907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G20300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGCGTTACTCCGATCAAAACTCCATCTTTCTCGAACCCTTTCCTTCATCTCTCCTCTTCTACCCAAAACCTTCTCCACCTCGGCAACTTCTCTACTCAGTGACCATGAAAACGACGAATCCGCCGCCACCATAACCGCCGCCGTCTCTGTCCCAATCTCACCTCTGTTAACTCCGGAAGACACACAAACCGTAGAAAAGTTCCATTCAATCATCAAAGACCACTATCGCAAAAACCCTACAAGCCCCAACGACGCCATCTTAAACCCTAGCTTAACTCTCCATGCTCTCTCCCTCGATTTCTCCCAAATTGAAACTTCCCAAGTCTCTCCCTCCGTCGTCCGATGCGTGATAGAGAAGTGCGGCAGTGTCCGCCACGGAATCCCTCTTCACCAATCTCTAGCTTTCTTCAATTGGGCAACTTCACGAGACGATTACGACCACAAATCACCTCATCCTTACAACGAAATGATCGATCTTTCAGGAAAGGTTAGACAATTCGACTTAGCTTGGCATTTGATTGATCTAATGAAATCCAGAAACGTAGAGATTTCGATTGAAACCTTCACGATTTTGATTAGACGTTATGTTAGAGCTGGATTAGCTTCAGAGGCTGTACATTGCTTTAATCGTATGGAAGATTACGGTTGCGTACCTGATAAAATCGCGTTTTCCATAGTTATTAGTAACCTTTCTAGGAAGAGGAGAGCTAGTGAAGCTCAGTCGTTTTTCGATAGTTTGAAAGATAGATTCGAGCCTGATGTTATAGTTTACACCAATTTGGTTCGTGGTTGGTGTAGAGCTGGTGAAATCTCTGAGGCAGAGAAAGTGTTCAAGGAGATGAAGTTGGCTGGGATTGAACCTAATGTGTATACTTACAGTATTGTGATCGATGCGTTGTGTAGATGCGGGCAAATCAGCCGTGCGCATGATGTGTTTGCGGATATGTTAGATTCAGGTTGTGCGCCGAATGCGATTACTTTTAACAACTTGATGAGGGTTCATGTGAAAGCAGGAAGAACAGAGAAGGTTTTGCAGGTTTATAACCAGATGAAGAAGCTAGGATGTGAGCCTGATACTATTACTTATAACTTCCTTATTGAGGCTCATTGCAGAGACGAGAATCTGGAAAACGCGGTCAAGGTTTTGAACACGATGATCAAGAAGAAGTGTGAAGTGAATGCTTCGACTTTCAATACGATATTTAGGTACATTGAGAAGAAGAGAGACGTGAATGGCGCTCATAGGATGTATTCGAAGATGATGGAAGCGAAATGTGAGCCGAATACGGTGACTTATAATATACTGATGAGGATGTTTGTCGGGTCAAAATCTACTGATATGGTTCTGAAGATGAAGAAAGAGATGGATGATAAAGAAGTTGAGCCGAATGTGAATACTTATAGGCTTTTGGTTACGATGTTTTGTGGGATGGGACATTGGAATAATGCTTATAAGTTGTTTAAAGAAATGGTTGAAGAGAAGTGTTTGACACCGAGCTTGTCTTTATATGAGATGGTTCTTGCTCAGTTAAGAAGAGCTGGGCAATTGAAGAAGCATGAAGAGCTGGTGGAGAAGATGATTCAGAAGGGACTCGTTGCTCGGCCATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

537

Amino Acids

61.21

Weight (kDa)

8.5

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	154 - 197	1.4e-07	PPR repeat family
PPR_3	PF13812	173 - 229	8.7e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	186 - 232	9.6e-09	PPR repeat family
TPR_24	PF23276	240 - 351	3.5e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	242 - 353	1.5e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	249 - 299	2.5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	251 - 282	3.2e-10	PPR repeat
PPR_2	PF13041	253 - 302	1.6e-18	PPR repeat family
PPR	PF01535	257 - 286	6e-07	PPR repeat
PPR_1	PF12854	285 - 316	6.4e-10	PPR repeat
PPR_3	PF13812	285 - 336	1.8e-09	Pentatricopeptide repeat domain
PPR	PF01535	291 - 321	1.6e-06	PPR repeat
PPR_2	PF13041	296 - 333	3.9e-09	PPR repeat family
PPR_3	PF13812	312 - 369	3.5e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 372	1.9e-16	PPR repeat family
PPR	PF01535	326 - 356	2.5e-06	PPR repeat
TPR_24	PF23276	342 - 447	8.2e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	347 - 405	2.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	355 - 386	7.3e-11	PPR repeat
PPR_2	PF13041	358 - 403	1.2e-12	PPR repeat family
PPR_long	PF17177	362 - 486	1.7e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	382 - 442	2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	394 - 440	1.1e-11	PPR repeat family
PPR_2	PF13041	428 - 476	5.8e-11	PPR repeat family
PPR_3	PF13812	452 - 508	2.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	459 - 492	9.1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014352)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20300
fragaria_vesca	FvH4_2g37970
prunus_persica	Prupe.1G359600_v2.0.a1
pyrus_communis	pycom08g00640 pycom15g00550
rosa_chinensis	RchiOBHm_Chr6g0303651
rosa_laevigata	RLG00000011063
rosa_multiflora	Rmu_co8419065.1_g000001 Rmu_sc0013443.1_g000006 Rmu_ssc0000114.1_g000016
rosa_roxburghii	Rroxscaffold_7G00164520
rosa_rugosa	Rorug06G0323400
rosa_samantha	Rh6AG437700 Rh6BG465400 Rh6CG449800 Rh6DG436600
rosa_wichuraiana	Rw6G037890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1044, 1089, 1299, 1455
Acc16I	TGCGCA	1 cut(s)	927
Acc36I	ACCTGC	1 cut(s)	1027
AccI	GTMKAC	1 cut(s)	872
AccII	CGCG	2 cut(s)	668, 1136
AccIII	TCCGGA	1 cut(s)	175
AciI	CCGC	9 cut(s)	123, 126, 138, 141, 363, 373, 909, 941, 1136
AcoI	YGGCCR	1 cut(s)	1604
AcuI	CTGAAG	2 cut(s)	591, 1370
AcyI	GRCGYC	1 cut(s)	255
AfaI	GTAC	3 cut(s)	618, 654, 1211
AfiI	CCNNNNNNNGG	1 cut(s)	1102
AjiI	CACGTC	1 cut(s)	1231
Alw26I	GTCTC	5 cut(s)	151, 331, 423, 1110, 1221
AlwNI	CAGNNNCTG	1 cut(s)	614
Aor13HI	TCCGGA	1 cut(s)	175
AoxI	GGCC	1 cut(s)	1604
Asp700I	GAANNNNTTC	2 cut(s)	40, 830
AspLEI	GCGC	3 cut(s)	928, 967, 1241
AsuHPI	GGTGA	5 cut(s)	153, 383, 441, 818, 1303
AsuII	TTCGAA	1 cut(s)	1256
BauI	CACGAG	1 cut(s)	426
BbsI	GAAGAC	1 cut(s)	186
BccI	CCATC	6 cut(s)	33, 266, 1258, 1360, 1426, 1510
BceAI	ACGGC	2 cut(s)	128, 906
BclI	TGATCA	1 cut(s)	1158
BcoDI	GTCTC	5 cut(s)	151, 331, 423, 1110, 1221
BfaI	CTAG	5 cut(s)	270, 401, 696, 711, 1061
BfoI	RGCGCY	1 cut(s)	1242
BfuAI	ACCTGC	1 cut(s)	1027
BisI	GCNGC	3 cut(s)	126, 141, 364
BlsI	GCNGC	3 cut(s)	127, 142, 365
BmgBI	CACGTC	1 cut(s)	1231
BmsI	GCATC	3 cut(s)	335, 883, 896
BpiI	GAAGAC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	1256
Bsa29I	ATCGAT	2 cut(s)	474, 891
BsaHI	GRCGYC	1 cut(s)	255
BsaJI	CCNNGG	2 cut(s)	81, 376
BsaWI	WCCGGW	1 cut(s)	175
BsaXI	ACNNNNNCTCC	4 cut(s)	697, 727, 830, 860
Bsc4I	CCNNNNNNNGG	1 cut(s)	1102
Bse3DI	GCAATG	2 cut(s)	619, 1108
BseAI	TCCGGA	1 cut(s)	175
BseCI	ATCGAT	2 cut(s)	474, 891
BseDI	CCNNGG	2 cut(s)	81, 376
BseGI	GGATG	6 cut(s)	454, 1070, 1254, 1320, 1375, 1437
BseLI	CCNNNNNNNGG	1 cut(s)	1102
BseMI	GCAATG	2 cut(s)	619, 1108
BseMII	CTCAG	4 cut(s)	112, 734, 807, 1541
BseRI	GAGGAG	2 cut(s)	45, 718
BseYI	CCCAGC	2 cut(s)	851, 1541
Bsh1236I	CGCG	2 cut(s)	668, 1136
BshFI	GGCC	1 cut(s)	1606
BshVI	ATCGAT	2 cut(s)	474, 891
BsiSI	CCGG	1 cut(s)	176
BslFI	GGGAC	3 cut(s)	137, 1449, 1605
BslI	CCNNNNNNNGG	1 cut(s)	1102
BsmAI	GTCTC	5 cut(s)	151, 331, 423, 1110, 1221
BsmBI	CGTCTC	4 cut(s)	151, 423, 1110, 1221
BsmFI	GGGAC	3 cut(s)	137, 1449, 1605
BsmI	GAATGC	2 cut(s)	976, 1186
BsnI	GGCC	1 cut(s)	1606
Bsp119I	TTCGAA	1 cut(s)	1256
Bsp13I	TCCGGA	1 cut(s)	175
Bsp1407I	TGTACA	1 cut(s)	616
Bsp143I	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
BspACI	CCGC	9 cut(s)	123, 126, 138, 141, 363, 373, 909, 941, 1136
BspANI	GGCC	1 cut(s)	1606
BspCNI	CTCAG	4 cut(s)	111, 733, 808, 1540
BspDI	ATCGAT	2 cut(s)	474, 891
BspEI	TCCGGA	1 cut(s)	175
BspFNI	CGCG	2 cut(s)	668, 1136
BspMI	ACCTGC	1 cut(s)	1027
BspQI	GCTCTTC	2 cut(s)	1531, 1557
BspT104I	TTCGAA	1 cut(s)	1256
BsrDI	GCAATG	2 cut(s)	619, 1108
BsrGI	TGTACA	1 cut(s)	616
BssECI	CCNNGG	2 cut(s)	81, 376
BssMI	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
BssNAI	GTATAC	1 cut(s)	873
BssNI	GRCGYC	1 cut(s)	255
BssSI	CACGAG	1 cut(s)	426
Bst1107I	GTATAC	1 cut(s)	873
Bst2BI	CACGAG	1 cut(s)	426
Bst4CI	ACNGT	4 cut(s)	193, 647, 881, 1291
Bst6I	CTCTTC	7 cut(s)	63, 393, 695, 1217, 1473, 1531, 1557
BstACI	GRCGYC	1 cut(s)	255
BstAUI	TGTACA	1 cut(s)	616
BstBI	TTCGAA	1 cut(s)	1256
BstC8I	GCNNGC	1 cut(s)	911
BstDEI	CTNAG	5 cut(s)	98, 505, 720, 816, 1527
BstDSI	CCRYGG	1 cut(s)	376
BstENI	CCTNNNNNAGG	1 cut(s)	1100
BstF5I	GGATG	6 cut(s)	454, 1070, 1254, 1320, 1375, 1437
BstFNI	CGCG	2 cut(s)	668, 1136
BstH2I	RGCGCY	1 cut(s)	1242
BstHHI	GCGC	3 cut(s)	928, 967, 1241
BstKTI	GATC	6 cut(s)	17, 474, 478, 526, 891, 1161
BstMAI	GTCTC	5 cut(s)	151, 331, 423, 1110, 1221
BstMBI	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
BstMWI	GCNNNNNNNGC	6 cut(s)	372, 602, 611, 716, 971, 1564
BstUI	CGCG	2 cut(s)	668, 1136
BstV2I	GAAGAC	1 cut(s)	186
BstZ17I	GTATAC	1 cut(s)	873
Bsu15I	ATCGAT	2 cut(s)	474, 891
BsuRI	GGCC	1 cut(s)	1606
BsuTUI	ATCGAT	2 cut(s)	474, 891
BtgI	CCRYGG	1 cut(s)	376
BtrI	CACGTC	1 cut(s)	1231
BtsCI	GGATG	6 cut(s)	454, 1070, 1254, 1320, 1375, 1437
BtsI	GCAGTG	1 cut(s)	373
BtsIMutI	CAGTG	2 cut(s)	106, 373
BveI	ACCTGC	1 cut(s)	1027
Cac8I	GCNNGC	1 cut(s)	911
CaiI	CAGNNNCTG	1 cut(s)	614
CfoI	GCGC	3 cut(s)	928, 967, 1241
ClaI	ATCGAT	2 cut(s)	474, 891
CseI	GACGC	1 cut(s)	263
Csp6I	GTAC	3 cut(s)	617, 653, 1210
CviAII	CATG	5 cut(s)	107, 284, 929, 1004, 1559
CviQI	GTAC	3 cut(s)	617, 653, 1210
DdeI	CTNAG	5 cut(s)	98, 505, 720, 816, 1527
DpnI	GATC	6 cut(s)	16, 473, 477, 525, 890, 1160
DpnII	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
DraI	TTTAAA	1 cut(s)	1465
EaeI	YGGCCR	1 cut(s)	1604
Eam1104I	CTCTTC	7 cut(s)	63, 393, 695, 1217, 1473, 1531, 1557
EarI	CTCTTC	7 cut(s)	63, 393, 695, 1217, 1473, 1531, 1557
EciI	GGCGGA	2 cut(s)	112, 362
Eco57I	CTGAAG	2 cut(s)	591, 1370
EcoNI	CCTNNNNNAGG	1 cut(s)	1100
Esp3I	CGTCTC	4 cut(s)	151, 423, 1110, 1221
FaeI	CATG	5 cut(s)	110, 287, 932, 1007, 1562
FaqI	GGGAC	3 cut(s)	137, 1449, 1605
FatI	CATG	5 cut(s)	106, 283, 928, 1003, 1558
FauI	CCCGC	1 cut(s)	902
FbaI	TGATCA	1 cut(s)	1158
FblI	GTMKAC	1 cut(s)	872
Fnu4HI	GCNGC	3 cut(s)	126, 141, 364
FokI	GGATG	6 cut(s)	441, 1077, 1261, 1327, 1382, 1444
Fsp4HI	GCNGC	3 cut(s)	126, 141, 364
FspAI	RTGCGCAY	1 cut(s)	927
FspBI	CTAG	5 cut(s)	270, 401, 696, 711, 1061
FspI	TGCGCA	1 cut(s)	927
GlaI	GCGC	3 cut(s)	927, 966, 1240
GluI	GCNGC	3 cut(s)	126, 141, 364
GsaI	CCCAGC	2 cut(s)	855, 1545
HaeII	RGCGCY	1 cut(s)	1242
HaeIII	GGCC	1 cut(s)	1606
HapII	CCGG	1 cut(s)	176
HgaI	GACGC	1 cut(s)	263
HhaI	GCGC	3 cut(s)	928, 967, 1241
Hin1I	GRCGYC	1 cut(s)	255
Hin1II	CATG	5 cut(s)	110, 287, 932, 1007, 1562
Hin6I	GCGC	3 cut(s)	926, 965, 1239
HinP1I	GCGC	3 cut(s)	926, 965, 1239
HincII	GTYRAC	1 cut(s)	171
HindII	GTYRAC	1 cut(s)	171
HinfI	GANTC	7 cut(s)	119, 381, 750, 953, 1123, 1582, 1593
HpaI	GTTAAC	1 cut(s)	171
HpaII	CCGG	1 cut(s)	176
HphI	GGTGA	5 cut(s)	153, 383, 441, 818, 1303
Hpy166II	GTNNAC	3 cut(s)	171, 772, 873
Hpy188I	TCNGA	6 cut(s)	14, 344, 610, 817, 1350, 1587
Hpy188III	TCNNGA	8 cut(s)	33, 176, 426, 483, 537, 568, 1127, 1162
Hpy8I	GTNNAC	3 cut(s)	171, 772, 873
Hpy99I	CGWCG	3 cut(s)	119, 257, 341
HpyAV	CCTTC	5 cut(s)	55, 82, 574, 1021, 1582
HpyCH4III	ACNGT	4 cut(s)	193, 647, 881, 1291
HpyCH4IV	ACGT	3 cut(s)	543, 583, 1230
HpyCH4V	TGCA	2 cut(s)	1036, 1113
HpyF10VI	GCNNNNNNNGC	6 cut(s)	372, 602, 611, 716, 971, 1564
HpyF3I	CTNAG	5 cut(s)	98, 505, 720, 816, 1527
HpySE526I	ACGT	3 cut(s)	543, 583, 1230
Hsp92I	GRCGYC	1 cut(s)	255
Hsp92II	CATG	5 cut(s)	110, 287, 932, 1007, 1562
HspAI	GCGC	3 cut(s)	926, 965, 1239
Kpn2I	TCCGGA	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	1158
KspAI	GTTAAC	1 cut(s)	171
Kzo9I	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
LguI	GCTCTTC	2 cut(s)	1531, 1557
LweI	GCATC	3 cut(s)	335, 883, 896
MaeI	CTAG	5 cut(s)	270, 401, 696, 711, 1061
MaeII	ACGT	3 cut(s)	543, 583, 1230
MaeIII	GTNAC	5 cut(s)	6, 101, 686, 1291, 1414
MalI	GATC	6 cut(s)	16, 473, 477, 525, 890, 1160
MboI	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
MfeI	CAATTG	2 cut(s)	412, 1547
MluCI	AATT	5 cut(s)	309, 412, 497, 778, 1547
MlyI	GAGTC	1 cut(s)	1587
MroI	TCCGGA	1 cut(s)	175
MroXI	GAANNNNTTC	2 cut(s)	40, 830
MseI	TTAA	7 cut(s)	170, 263, 275, 627, 984, 1464, 1532
MspI	CCGG	1 cut(s)	176
MunI	CAATTG	2 cut(s)	412, 1547
Mva1269I	GAATGC	2 cut(s)	976, 1186
MvnI	CGCG	2 cut(s)	668, 1136
MwoI	GCNNNNNNNGC	6 cut(s)	372, 602, 611, 716, 971, 1564
NdeII	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
NlaIII	CATG	5 cut(s)	110, 287, 932, 1007, 1562
NmeAIII	GCCGAG	2 cut(s)	62, 1582
NmuCI	GTSAC	2 cut(s)	101, 1291
NsbI	TGCGCA	1 cut(s)	927
NspV	TTCGAA	1 cut(s)	1256
PciSI	GCTCTTC	2 cut(s)	1531, 1557
PcsI	WCGNNNNNNNCGW	2 cut(s)	345, 471
PctI	GAATGC	2 cut(s)	976, 1186
PdmI	GAANNNNTTC	2 cut(s)	40, 830
PfeI	GAWTC	6 cut(s)	119, 381, 750, 953, 1123, 1582
PkrI	GCNGC	3 cut(s)	127, 142, 365
PleI	GAGTC	1 cut(s)	1587
PpsI	GAGTC	1 cut(s)	1587
PsiI	TTATAA	4 cut(s)	1044, 1089, 1299, 1455
PspFI	CCCAGC	2 cut(s)	851, 1541
PstNI	CAGNNNCTG	1 cut(s)	614
RsaI	GTAC	3 cut(s)	618, 654, 1211
RsaNI	GTAC	3 cut(s)	617, 653, 1210
SapI	GCTCTTC	2 cut(s)	1531, 1557
SaqAI	TTAA	7 cut(s)	170, 263, 275, 627, 984, 1464, 1532
SatI	GCNGC	3 cut(s)	126, 141, 364
Sau3AI	GATC	6 cut(s)	14, 471, 475, 523, 888, 1158
SchI	GAGTC	1 cut(s)	1587
SfaNI	GCATC	3 cut(s)	335, 883, 896
SfuI	TTCGAA	1 cut(s)	1256
Sse9I	AATT	5 cut(s)	309, 412, 497, 778, 1547
SsiI	CCGC	9 cut(s)	123, 126, 138, 141, 363, 373, 909, 941, 1136
SspMI	CTAG	5 cut(s)	270, 401, 696, 711, 1061
TaaI	ACNGT	4 cut(s)	193, 647, 881, 1291
TaiI	ACGT	3 cut(s)	546, 586, 1233
TasI	AATT	5 cut(s)	309, 412, 497, 778, 1547
TatI	WGTACW	1 cut(s)	616
TauI	GCSGC	3 cut(s)	128, 143, 366
TfiI	GAWTC	6 cut(s)	119, 381, 750, 953, 1123, 1582
Tru1I	TTAA	7 cut(s)	170, 263, 275, 627, 984, 1464, 1532
Tru9I	TTAA	7 cut(s)	170, 263, 275, 627, 984, 1464, 1532
TscAI	CASTG	2 cut(s)	106, 373
TseFI	GTSAC	2 cut(s)	101, 1291
Tsp45I	GTSAC	2 cut(s)	101, 1291
TspDTI	ATGAA	8 cut(s)	37, 123, 545, 857, 992, 1067, 1370, 1575
TspGWI	ACGGA	2 cut(s)	325, 393
TspRI	CASTG	2 cut(s)	106, 373
XagI	CCTNNNNNAGG	1 cut(s)	1100
XmiI	GTMKAC	1 cut(s)	872
XmnI	GAANNNNTTC	2 cut(s)	40, 830
XspI	CTAG	5 cut(s)	270, 401, 696, 711, 1061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.