AT1G22750

Protein of unknown function (DUF1475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007457
Physical Location & Seq
Forward (+)
8050770 .. 8052930
2161 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22750.1

Sequence Viewer

Length: 735 bp
ATGGCGACGACTAGTTTAGTGACCGGACTAAAAGTGGTATTACCGGTTATGTTCTGTCTGATGCTAGCGACTCTTGTCTACACCATCATCACCGATGGCCTTCCTTTACCTGATCGTCAAGATGTCTTCACACCATGGTTCGTTACGACAATTTTGGATTTCTACATTAATCTTGTACCTATAGCGGTTTGGATTGTGTATAAGGAGTCTACATGGTCTGGTTCAATACTTTGGACCATTTTGCTCATCATTTTTGGCAGCCTCACGACATGTGTGTATCTGTTTCTGCAACTTCTCAAGCTGACAAATCAAGAAGCTTCAGAAGATCCTATGTACTATTTGTTACTTCGAGATTCAATCAAGGATGGAGTTGGTCTGAGAGACAAGAATTCACTTGTTGTCACTGCAAGATTTGTGTTTGGTGCCTTAGGATGTGTGATGCTAGGAGCTTTGGTTTACACTTGCTTCACTTATGGTTCTCCTTTCCACATGGAGCTTCTATACCCGTGGATGGTGGTTTTATTGGTTAACTTCTACATTGATGTTGCTGTCTTATCAGTTTGGGTTGTCTATAAAGAATCTAGTTTGATCATTGGAATTCTATGGGTTGCTCTATTGATAGGTCTTGGCAGCGTTGGCACGAGCGCAGTCATTGTCGTGCAGCTATTCCGTCTGTCTCCTCTCGACCCGCTCTACCTCGTTCTAGTGAACAACAGCAATCGGAAGCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1475

Protein Analysis

244

Amino Acids

27.33

Weight (kDa)

5.29

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1475 PF07343 2 - 241 2.6e-60 Protein of unknown function (DUF1475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 691
AccI GTMKAC 2 cut(s) 78, 209
AciI CCGC 2 cut(s) 185, 689
AclWI GGATC 1 cut(s) 320
AcsI RAATTY 2 cut(s) 388, 597
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 2 cut(s) 177, 335
AfiI CCNNNNNNNGG 1 cut(s) 511
AflIII ACRYGT 1 cut(s) 269
AgeI ACCGGT 1 cut(s) 43
AgsI TTSAA 2 cut(s) 225, 357
AhlI ACTAGT 1 cut(s) 11
AluBI AGCT 5 cut(s) 301, 317, 449, 496, 664
AluI AGCT 5 cut(s) 301, 317, 449, 496, 664
Alw26I GTCTC 2 cut(s) 375, 681
AlwI GGATC 1 cut(s) 320
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 258, 630, 661
ApoI RAATTY 2 cut(s) 388, 597
AseI ATTAAT 1 cut(s) 168
AsiGI ACCGGT 1 cut(s) 43
AspLEI GCGC 1 cut(s) 647
AspS9I GGNCC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 82
AsuNHI GCTAGC 1 cut(s) 64
AvaII GGWCC 1 cut(s) 234
AxyI CCTNAGG 1 cut(s) 427
BanI GGYRCC 1 cut(s) 422
BauI CACGAG 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 270, 642, 673
BccI CCATC 4 cut(s) 89, 92, 359, 505
BclI TGATCA 1 cut(s) 588
BcoDI GTCTC 2 cut(s) 375, 681
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 5 cut(s) 12, 65, 443, 582, 704
BfmI CTRYAG 1 cut(s) 180
BisI GCNGC 3 cut(s) 259, 631, 662
BlsI GCNGC 3 cut(s) 260, 632, 663
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 424
BmsI GCATC 2 cut(s) 51, 429
BmtI GCTAGC 1 cut(s) 68
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 134, 506
BsaWI WCCGGW 2 cut(s) 23, 43
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 1 cut(s) 511
Bse118I RCCGGY 1 cut(s) 43
Bse21I CCTNAGG 1 cut(s) 427
BseDI CCNNGG 2 cut(s) 134, 506
BseGI GGATG 3 cut(s) 370, 437, 516
BseLI CCNNNNNNNGG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 669
BseXI GCAGC 3 cut(s) 270, 642, 673
BsgI GTGCAG 1 cut(s) 680
BshFI GGCC 1 cut(s) 99
BshNI GGYRCC 1 cut(s) 422
BshTI ACCGGT 1 cut(s) 43
BsiSI CCGG 2 cut(s) 24, 44
BslI CCNNNNNNNGG 1 cut(s) 511
BsmAI GTCTC 2 cut(s) 375, 681
BsnI GGCC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 112, 325, 588
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 2 cut(s) 185, 689
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 424
BspOI GCTAGC 1 cut(s) 68
BspPI GGATC 1 cut(s) 320
BspT107I GGYRCC 1 cut(s) 422
BsrBI CCGCTC 1 cut(s) 691
BsrFI RCCGGY 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 134, 506
BssMI GATC 3 cut(s) 112, 325, 588
BssSI CACGAG 1 cut(s) 640
BssT1I CCWWGG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 640
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 377, 427
BstDSI CCRYGG 2 cut(s) 134, 506
BstF5I GGATG 3 cut(s) 370, 437, 516
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 3 cut(s) 115, 328, 591
BstMAI GTCTC 2 cut(s) 375, 681
BstMBI GATC 3 cut(s) 112, 325, 588
BstMWI GCNNNNNNNGC 1 cut(s) 636
BstNSI RCATGY 1 cut(s) 273
BstPAI GACNNNNGTC 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 270, 642, 673
BstV2I GAAGAC 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 427
BsuRI GGCC 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 134, 506
BtsCI GGATG 3 cut(s) 370, 437, 516
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 66
CfoI GCGC 1 cut(s) 647
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 176, 334
CspAI ACCGGT 1 cut(s) 43
CviAII CATG 4 cut(s) 135, 213, 270, 490
CviJI RGCY 7 cut(s) 99, 261, 301, 317, 449, 496, 664
CviKI_1 RGCY 7 cut(s) 99, 261, 301, 317, 449, 496, 664
CviQI GTAC 2 cut(s) 176, 334
DdeI CTNAG 2 cut(s) 377, 427
DpnI GATC 3 cut(s) 114, 327, 590
DpnII GATC 3 cut(s) 112, 325, 588
Eco130I CCWWGG 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 427
EcoRI GAATTC 2 cut(s) 388, 597
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 4 cut(s) 138, 216, 273, 493
FatI CATG 4 cut(s) 134, 212, 269, 489
FauI CCCGC 1 cut(s) 696
FbaI TGATCA 1 cut(s) 588
FblI GTMKAC 2 cut(s) 78, 209
Fnu4HI GCNGC 3 cut(s) 259, 631, 662
FokI GGATG 3 cut(s) 377, 444, 523
Fsp4HI GCNGC 3 cut(s) 259, 631, 662
FspBI CTAG 5 cut(s) 12, 65, 443, 582, 704
GlaI GCGC 1 cut(s) 646
GluI GCNGC 3 cut(s) 259, 631, 662
HaeIII GGCC 1 cut(s) 99
HapII CCGG 2 cut(s) 24, 44
HhaI GCGC 1 cut(s) 647
Hin1II CATG 4 cut(s) 138, 216, 273, 493
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 4 cut(s) 70, 206, 353, 578
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 2 cut(s) 24, 44
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 5 cut(s) 79, 210, 457, 529, 709
Hpy188I TCNGA 4 cut(s) 60, 322, 378, 723
Hpy188III TCNNGA 5 cut(s) 119, 265, 311, 350, 683
Hpy8I GTNNAC 5 cut(s) 79, 210, 457, 529, 709
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 110
HpyCH4V TGCA 3 cut(s) 289, 407, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 636
HpyF3I CTNAG 2 cut(s) 377, 427
Hsp92II CATG 4 cut(s) 138, 216, 273, 493
HspAI GCGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 588
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 3 cut(s) 112, 325, 588
LmnI GCTCC 2 cut(s) 446, 493
LpnPI CCDG 4 cut(s) 37, 57, 123, 204
Lsp1109I GCAGC 3 cut(s) 270, 642, 673
LweI GCATC 2 cut(s) 51, 429
MaeI CTAG 5 cut(s) 12, 65, 443, 582, 704
MaeIII GTNAC 4 cut(s) 19, 142, 342, 400
MalI GATC 3 cut(s) 114, 327, 590
MbiI CCGCTC 1 cut(s) 691
MboI GATC 3 cut(s) 112, 325, 588
MboII GAAGA 2 cut(s) 118, 335
MflI RGATCY 1 cut(s) 325
MluCI AATT 3 cut(s) 150, 388, 597
MlyI GAGTC 2 cut(s) 64, 215
MnlI CCTC 3 cut(s) 272, 690, 707
MseI TTAA 3 cut(s) 168, 528, 733
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 2 cut(s) 24, 44
MwoI GCNNNNNNNGC 1 cut(s) 636
NcoI CCATGG 1 cut(s) 134
NdeII GATC 3 cut(s) 112, 325, 588
NheI GCTAGC 1 cut(s) 64
NlaIII CATG 4 cut(s) 138, 216, 273, 493
NlaIV GGNNCC 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 19, 400
NspI RCATGY 1 cut(s) 273
PciI ACATGT 1 cut(s) 269
PfeI GAWTC 2 cut(s) 353, 578
PinAI ACCGGT 1 cut(s) 43
PkrI GCNGC 3 cut(s) 260, 632, 663
PleI GAGTC 2 cut(s) 64, 214
PpsI GAGTC 2 cut(s) 64, 214
PscI ACATGT 1 cut(s) 269
PshAI GACNNNNGTC 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 177, 335
RsaNI GTAC 2 cut(s) 176, 334
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 3 cut(s) 168, 528, 733
SatI GCNGC 3 cut(s) 259, 631, 662
Sau3AI GATC 3 cut(s) 112, 325, 588
Sau96I GGNCC 1 cut(s) 234
SchI GAGTC 2 cut(s) 64, 215
SetI ASST 9 cut(s) 112, 181, 303, 319, 451, 498, 625, 666, 699
SfaNI GCATC 2 cut(s) 51, 429
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 3 cut(s) 150, 388, 597
SsiI CCGC 2 cut(s) 185, 689
SspMI CTAG 5 cut(s) 12, 65, 443, 582, 704
StyI CCWWGG 1 cut(s) 134
TaqI TCGA 2 cut(s) 349, 684
TasI AATT 3 cut(s) 150, 388, 597
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 2 cut(s) 353, 578
Tru1I TTAA 3 cut(s) 168, 528, 733
Tru9I TTAA 3 cut(s) 168, 528, 733
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 2 cut(s) 19, 400
TseI GCWGC 3 cut(s) 258, 630, 661
Tsp45I GTSAC 2 cut(s) 19, 400
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 234
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 2 cut(s) 388, 597
XceI RCATGY 1 cut(s) 273
XmiI GTMKAC 2 cut(s) 78, 209
XspI CTAG 5 cut(s) 12, 65, 443, 582, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.