AT1G26520

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011931
Physical Location & Seq
Reverse (-)
9163211 .. 9165900
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G26520.1

Sequence Viewer

Length: 1125 bp
ATGCTGAAGGAAGAAGCTGAGGGAGTGAAAATGGAAGATGATGATGAAGAACCACCGATGGCTGTTCAAATCCATCCGGATGTTTCTGTCGGAAAGGTTCACTCATCAAGCGATACTGTTTCAGTTGGTGTGTCTGTTATCACTGGCTATCTCGGCGCGGGAAAATCCACTCTTGTGAATTATATATTGAACGGGAAACACGGGAAGAGAATAGCTGTGATTTTGAATGAGTTTGGGGAAGAGATTGGAGTTGAGAGGGCTATGATTAACGAAGGAGAAGAAGGTGCCATTGTTGAAGAATGGGTTGAGCTCGCTAATGGCTGTGTTTGTTGTACTGTCAAGCATAGTCTTGTTCAAGCTCTCGAGCAGCTTGTTCAGAGAAAAGACAGGCTTGATCATATATTATTGGAGACAACAGGTTTAGCAAACCCAGCTCCATTGGCTTCTATTCTTTGGTTGGATGACCAGTTAGAATCAGAAGTCAAGCTTGACTGTATTGTTACTGTCGTGGATGCAAAGAATCTTCGTTTTCAGCTCAATGAGCGTCGTGACTCATCCTCGTTTCCTGAGGCCTTTAATCAAATAGCATTTGCGGACACGATAATAATGAACAAGGTGGACTTGATTTCTCAAGAGGAGTCTGATGAATTAGAGAAGGAGATTCATTCCATCAACTCGCTCGCCAATGTCATCCGATCTGTTAGGTGCCAAGTTGATTTGTCCAATATATTAAACTGCCAGGCATATGATTCAACTCATGTTAGTCGTCTGGAATCCCTGCTGGAAGCAAATAAATCATTAACGACGACAGATCTTCACGATAGTGGAGTAAGGACTCTATGCATCAGCGAACCGCAACCTATAAACCTCGATAAGGTTCGTTTATGGCTGGAGGAAATTCTGTGGGATAAAAAATCGGAGATGGATGTATACCGTTGCAAGGCTGTGTTGAGCATTCAAAACTCAGACCAGATGCATATTTTGCAGGCCGTTAGAGATATATATGAGATAGTGCCAGCTCGAAAATGGAGCGAGGAAGAGAATCGAACAAACAAAATAGTATTTATAGGACACAAACTGGATGAAGAAGTTCTCAGAAGCGGATTAAGAGATTGCAGACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C KTI12 cobW

Protein Analysis

374

Amino Acids

42.01

Weight (kDa)

4.78

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 44 - 231 4.6e-58 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 278 - 373 8.5e-19 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26520
fragaria_vesca FvH4_4g30300
malus_domestica MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica Prupe.1G284700_v2.0.a1
pyrus_communis pycom16g05480
rosa_chinensis RchiOBHm_Chr4g0438581
rosa_laevigata RLG00000006349
rosa_multiflora Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii Rroxscaffold_5G00379590
rosa_rugosa Rorug04G0311700
rosa_samantha Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 284, 705
AccI GTMKAC 1 cut(s) 930
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 4 cut(s) 158, 593, 854, 1101
AcsI RAATTY 1 cut(s) 897
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 334
AfiI CCNNNNNNNGG 2 cut(s) 874, 940
AgsI TTSAA 7 cut(s) 68, 190, 226, 296, 356, 753, 959
AjnI CCWGG 1 cut(s) 738
AleI CACNNNNGTG 2 cut(s) 173, 822
AluBI AGCT 9 cut(s) 17, 215, 310, 359, 370, 434, 487, 535, 1019
AluI AGCT 9 cut(s) 17, 215, 310, 359, 370, 434, 487, 535, 1019
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 570, 987
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 897
Asp700I GAANNNNTTC 1 cut(s) 1089
AspLEI GCGC 1 cut(s) 158
AvaI CYCGRG 1 cut(s) 362
AxyI CCTNAGG 1 cut(s) 567
BanI GGYRCC 2 cut(s) 284, 705
BanII GRGCYC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 312
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 379
BccI CCATC 4 cut(s) 52, 81, 677, 916
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 1 cut(s) 740
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 1 cut(s) 1123
BglII AGATCT 1 cut(s) 811
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 740
BmeT110I CYCGRG 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 286, 707
BmrFI CCNGG 1 cut(s) 740
BmsI GCATC 3 cut(s) 502, 852, 963
BpmI CTGGAG 1 cut(s) 911
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 615
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 874, 940
Bse1I ACTGG 3 cut(s) 148, 466, 1083
Bse21I CCTNAGG 1 cut(s) 567
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 1 cut(s) 740
BseGI GGATG 8 cut(s) 73, 85, 466, 517, 554, 690, 931, 1087
BseLI CCNNNNNNNGG 2 cut(s) 874, 940
BseMII CTCAG 4 cut(s) 9, 558, 978, 1108
BseNI ACTGG 3 cut(s) 148, 466, 1083
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 430
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 572, 989
BshNI GGYRCC 2 cut(s) 284, 705
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 874, 940
BsmAI GTCTC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 2 cut(s) 572, 989
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 76
Bsp143I GATC 3 cut(s) 394, 695, 811
BspACI CCGC 4 cut(s) 158, 593, 854, 1101
BspANI GGCC 2 cut(s) 572, 989
BspCNI CTCAG 4 cut(s) 10, 559, 977, 1107
BspEI TCCGGA 1 cut(s) 76
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 2 cut(s) 286, 707
BspT107I GGYRCC 2 cut(s) 284, 705
BsrI ACTGG 3 cut(s) 148, 466, 1083
BssMI GATC 3 cut(s) 394, 695, 811
BssNAI GTATAC 1 cut(s) 931
Bst1107I GTATAC 1 cut(s) 931
Bst2UI CCWGG 1 cut(s) 740
Bst4CI ACNGT 5 cut(s) 118, 337, 494, 505, 935
Bst6I CTCTTC 3 cut(s) 200, 234, 1032
BstAPI GCANNNNNTGC 1 cut(s) 982
BstC8I GCNNGC 4 cut(s) 312, 681, 987, 1017
BstDEI CTNAG 4 cut(s) 18, 567, 964, 1094
BstENI CCTNNNNNAGG 1 cut(s) 872
BstF5I GGATG 8 cut(s) 73, 85, 466, 517, 554, 690, 931, 1087
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 3 cut(s) 397, 698, 814
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 3 cut(s) 394, 695, 811
BstMWI GCNNNNNNNGC 5 cut(s) 153, 431, 440, 541, 982
BstNI CCWGG 1 cut(s) 740
BstSCI CCNGG 1 cut(s) 738
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 811
BstYI RGATCY 1 cut(s) 811
BstZ17I GTATAC 1 cut(s) 931
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 2 cut(s) 572, 989
BtsCI GGATG 8 cut(s) 73, 85, 466, 517, 554, 690, 931, 1087
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 312, 681, 987, 1017
CfoI GCGC 1 cut(s) 158
CseI GACGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 333
CviAII CATG 1 cut(s) 758
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 4 cut(s) 18, 567, 964, 1094
DpnI GATC 3 cut(s) 396, 697, 813
DpnII GATC 3 cut(s) 394, 695, 811
Eam1104I CTCTTC 3 cut(s) 200, 234, 1032
EarI CTCTTC 3 cut(s) 200, 234, 1032
Ecl136II GAGCTC 1 cut(s) 310
Eco147I AGGCCT 1 cut(s) 572
Eco24I GRGCYC 1 cut(s) 312
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 26
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 872
EcoRII CCWGG 1 cut(s) 738
EcoT22I ATGCAT 2 cut(s) 845, 978
EcoT38I GRGCYC 1 cut(s) 312
FaeI CATG 1 cut(s) 761
FalI AAGNNNNNCTT 6 cut(s) 375, 407, 471, 503, 605, 637
FatI CATG 1 cut(s) 757
FauI CCCGC 1 cut(s) 151
FauNDI CATATG 1 cut(s) 745
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 8 cut(s) 60, 92, 473, 524, 541, 677, 938, 1094
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 1 cut(s) 1123
GlaI GCGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 368
GsaI CCCAGC 1 cut(s) 434
GsuI CTGGAG 1 cut(s) 911
HaeIII GGCC 2 cut(s) 572, 989
HapII CCGG 1 cut(s) 77
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 158
Hin1II CATG 1 cut(s) 761
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 9 cut(s) 473, 520, 551, 638, 661, 749, 773, 835, 1042
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 100, 619, 931
Hpy188I TCNGA 8 cut(s) 92, 378, 478, 643, 695, 919, 967, 1097
Hpy188III TCNNGA 7 cut(s) 77, 362, 548, 566, 632, 770, 818
Hpy8I GTNNAC 3 cut(s) 100, 619, 931
Hpy99I CGWCG 2 cut(s) 549, 808
HpyAV CCTTC 3 cut(s) 266, 275, 649
HpyCH4III ACNGT 5 cut(s) 118, 337, 494, 505, 935
HpyCH4V TGCA 6 cut(s) 515, 843, 939, 976, 985, 1116
HpyF10VI GCNNNNNNNGC 5 cut(s) 153, 431, 440, 541, 982
HpyF3I CTNAG 4 cut(s) 18, 567, 964, 1094
Hsp92II CATG 1 cut(s) 761
HspAI GCGC 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 3 cut(s) 394, 695, 811
LmnI GCTCC 2 cut(s) 439, 1029
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 502, 852, 963
MaeI CTAG 1 cut(s) 1123
MaeIII GTNAC 2 cut(s) 499, 548
MalI GATC 3 cut(s) 396, 697, 813
MboI GATC 3 cut(s) 394, 695, 811
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 3 cut(s) 178, 647, 897
MlyI GAGTC 3 cut(s) 545, 647, 829
MmeI TCCRAC 2 cut(s) 70, 438
MnlI CCTC 8 cut(s) 13, 249, 562, 568, 628, 878, 886, 1027
Mph1103I ATGCAT 2 cut(s) 845, 978
MroI TCCGGA 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 5 cut(s) 267, 576, 731, 800, 1106
MslI CAYNNNNRTG 3 cut(s) 78, 173, 822
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 740
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 1 cut(s) 740
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 153, 431, 440, 541, 982
NdeI CATATG 1 cut(s) 745
NdeII GATC 3 cut(s) 394, 695, 811
NlaIII CATG 1 cut(s) 761
NlaIV GGNNCC 2 cut(s) 286, 707
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 548
NsiI ATGCAT 2 cut(s) 845, 978
OliI CACNNNNGTG 2 cut(s) 173, 822
PaeR7I CTCGAG 1 cut(s) 362
PceI AGGCCT 1 cut(s) 572
PctI GAATGC 1 cut(s) 954
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 6 cut(s) 473, 520, 661, 749, 773, 1042
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 3 cut(s) 545, 646, 829
PpsI GAGTC 3 cut(s) 545, 646, 829
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 738
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 738
PspN4I GGNNCC 2 cut(s) 286, 707
PsuI RGATCY 1 cut(s) 811
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
RseI CAYNNNNRTG 3 cut(s) 78, 173, 822
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 5 cut(s) 267, 576, 731, 800, 1106
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 3 cut(s) 394, 695, 811
SchI GAGTC 3 cut(s) 545, 647, 829
ScrFI CCNGG 1 cut(s) 740
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 3 cut(s) 502, 852, 963
Sfr274I CTCGAG 1 cut(s) 362
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 3 cut(s) 78, 173, 822
SmlI CTYRAG 2 cut(s) 362, 630
SmoI CTYRAG 2 cut(s) 362, 630
Sse9I AATT 3 cut(s) 178, 647, 897
SseBI AGGCCT 1 cut(s) 572
SsiI CCGC 4 cut(s) 158, 593, 854, 1101
SspMI CTAG 1 cut(s) 1123
SstI GAGCTC 1 cut(s) 312
StuI AGGCCT 1 cut(s) 572
StyD4I CCNGG 1 cut(s) 738
TaaI ACNGT 5 cut(s) 118, 337, 494, 505, 935
TaqI TCGA 4 cut(s) 363, 870, 1021, 1045
TasI AATT 3 cut(s) 178, 647, 897
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 6 cut(s) 473, 520, 661, 749, 773, 1042
Tru1I TTAA 5 cut(s) 267, 576, 731, 800, 1106
Tru9I TTAA 5 cut(s) 267, 576, 731, 800, 1106
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 5 cut(s) 60, 623, 653, 660, 1098
TspRI CASTG 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 872
XapI RAATTY 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 1023
XhoI CTCGAG 1 cut(s) 362
XmiI GTMKAC 1 cut(s) 930
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 1 cut(s) 1123
Zsp2I ATGCAT 2 cut(s) 845, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.