Rh4CG387400

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011931
Physical Location & Seq
Reverse (-)
63900584 .. 63904003
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG387400.1

Sequence Viewer

Length: 1101 bp
ATGGAGGACGAAGAAGAAGCTCCACCTCTTGCCGTTGAAATCGACCAACCCTTTTCAAGTGGACCCGATGACGTCGACGCTCCCGTCGGCGTCACCGTCATCACTGGTTATCTGGGTTCAGGCAAATCCACTCTGGTTAATCATATATTAAACACCCAACATGGGAAGAAAATTGCTGTGATATTAAACGAGTTTGGTGAGGAAATTGGGGTTGAGAGGGCAATGATAAACGAAGGAGATGGTGGTGCTCTTGTGGAAGAGTGGGTTGAGTTGGCAAATGGGTGTATATGTTGCACTGTGAAGCACAGTTTGGTGCAAGCACTAGAGCAACTTGTGCAGAGAAAAGAAAGACTTGATCATATATTGTTGGAGACTACTGGGTTGGCTAACCCTGCTCCACTTGCTTCGGTTCTGTGGTTGGATGATCAGTTGGAATCGACTGTCAAGCTCGATTCGATTATCACTGTTGTGGATGCCAAGAACCTCCGTTTTCAGCTGAGCGAGAATAAAAGTTCATCCTCATTTCCTGAAGCATATCTGCAGATTGCATTTGCGGATGTTGTAATACTTAACAAGGTTGATTTGGTTTCTCCAGAGGGTTCTAAAGATTATCTGGAGGAACTCGAGAAGGAGATACATAGTATTAATTCGCTTGCTTGTATCATCCATTCTGTTCGTTGTCAAGTCGACTTGTCTAAGATATTGGACTGTCGGGCCTATGATGCTATGCATGCTGTTCATTTAGAATCATTATTGGAAGAAAGTCGTACTTTATCTACATGGGATCTCCATGATAGTGGTGTGCGAACCTTATGCATTAGTGAGCAACAGAAGGTTGATCTTGAAAAGGTCCGCTTATGGCTCGAGGAGATTCTTTGGGAAAAGAAATATGGCATGGATGTGTATCGCTGCAAAGGGGTTTTGAGTGTTCATAGTTCAAACCAATTACATACTTTGCAGGCAGTGAGGGAAATATATGAGATTGTGCCAACTCGTGATTGGCGAAAGGAAGAAAATCAGATGAACAAGATAGTGTTTATAGGGCATGATCTAAACGAGAATATTCTTACTGAGTCGTTTAGAGCATGCATAGTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C KTI12 cobW

Protein Analysis

366

Amino Acids

41.06

Weight (kDa)

4.75

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 31 - 219 5.4e-57 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 269 - 363 1.6e-18 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26520
fragaria_vesca FvH4_4g30300
malus_domestica MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica Prupe.1G284700_v2.0.a1
pyrus_communis pycom16g05480
rosa_chinensis RchiOBHm_Chr4g0438581
rosa_laevigata RLG00000006349
rosa_multiflora Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii Rroxscaffold_5G00379590
rosa_rugosa Rorug04G0311700
rosa_samantha Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AatII GACGTC 1 cut(s) 75
AccI GTMKAC 2 cut(s) 75, 687
AciI CCGC 2 cut(s) 554, 853
AclWI GGATC 1 cut(s) 792
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 2 cut(s) 72, 90
AfaI GTAC 1 cut(s) 769
AfiI CCNNNNNNNGG 1 cut(s) 162
AgsI TTSAA 4 cut(s) 38, 57, 845, 939
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 3 cut(s) 20, 448, 496
AluI AGCT 3 cut(s) 20, 448, 496
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 792
Ama87I CYCGRG 2 cut(s) 623, 863
AoxI GGCC 1 cut(s) 714
ApeKI GCWGC 1 cut(s) 909
AseI ATTAAT 1 cut(s) 645
AspS9I GGNCC 3 cut(s) 62, 714, 850
AsuHPI GGTGA 2 cut(s) 85, 209
AvaI CYCGRG 2 cut(s) 623, 863
AvaII GGWCC 2 cut(s) 62, 850
BauI CACGAG 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 896
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 17
BcgI CGANNNNNNTGC 2 cut(s) 795, 829
BclI TGATCA 2 cut(s) 355, 424
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 539
BisI GCNGC 1 cut(s) 910
BlpI GCTNAGC 1 cut(s) 497
BlsI GCNGC 1 cut(s) 911
Bme18I GGWCC 2 cut(s) 62, 850
BmeT110I CYCGRG 2 cut(s) 623, 863
BmgT120I GGNCC 3 cut(s) 62, 714, 850
BmiI GGNNCC 1 cut(s) 64
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 2 cut(s) 463, 712
BmuI ACTGGG 1 cut(s) 387
BpmI CTGGAG 2 cut(s) 576, 635
Bpu1102I GCTNAGC 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 903
BsaHI GRCGYC 2 cut(s) 72, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 2 cut(s) 109, 382
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 903
BseGI GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 488, 1062
BseNI ACTGG 2 cut(s) 109, 382
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 1 cut(s) 896
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 1 cut(s) 716
BsiHKAI GWGCWC 1 cut(s) 250
BsiHKCI CYCGRG 2 cut(s) 623, 863
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 1 cut(s) 716
BsoBI CYCGRG 2 cut(s) 623, 863
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 5 cut(s) 355, 424, 784, 838, 1048
Bsp1720I GCTNAGC 1 cut(s) 497
BspACI CCGC 2 cut(s) 554, 853
BspANI GGCC 1 cut(s) 716
BspCNI CTCAG 2 cut(s) 489, 1063
BspLI GGNNCC 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 543
BspPI GGATC 1 cut(s) 792
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 109, 382
BssMI GATC 5 cut(s) 355, 424, 784, 838, 1048
BssNI GRCGYC 2 cut(s) 72, 90
BssSI CACGAG 1 cut(s) 993
Bst2BI CACGAG 1 cut(s) 993
Bst4CI ACNGT 6 cut(s) 97, 298, 308, 442, 466, 710
Bst6I CTCTTC 1 cut(s) 252
BstACI GRCGYC 2 cut(s) 72, 90
BstAPI GCANNNNNTGC 1 cut(s) 334
BstC8I GCNNGC 5 cut(s) 318, 654, 732, 960, 1087
BstDEI CTNAG 3 cut(s) 497, 696, 1071
BstF5I GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BstKTI GATC 5 cut(s) 358, 427, 787, 841, 1051
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 5 cut(s) 355, 424, 784, 838, 1048
BstMWI GCNNNNNNNGC 5 cut(s) 334, 392, 401, 722, 731
BstNSI RCATGY 2 cut(s) 734, 1089
BstSFI CTRYAG 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 784
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 1 cut(s) 784
BsuRI GGCC 1 cut(s) 716
BtsCI GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 4 cut(s) 102, 294, 462, 969
Cac8I GCNNGC 5 cut(s) 318, 654, 732, 960, 1087
Cfr13I GGNCC 3 cut(s) 62, 714, 850
CseI GACGC 2 cut(s) 79, 86
Csp6I GTAC 1 cut(s) 768
CviAII CATG 7 cut(s) 161, 731, 780, 791, 895, 1046, 1086
CviJI RGCY 6 cut(s) 20, 386, 448, 496, 716, 862
CviKI_1 RGCY 6 cut(s) 20, 386, 448, 496, 716, 862
CviQI GTAC 1 cut(s) 768
DdeI CTNAG 3 cut(s) 497, 696, 1071
DpnI GATC 5 cut(s) 357, 426, 786, 840, 1050
DpnII GATC 5 cut(s) 355, 424, 784, 838, 1048
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 62, 850
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 2 cut(s) 623, 863
EcoT22I ATGCAT 3 cut(s) 732, 818, 1091
FaeI CATG 7 cut(s) 164, 734, 783, 794, 898, 1049, 1089
FalI AAGNNNNNCTT 6 cut(s) 336, 368, 754, 786, 839, 871
FatI CATG 7 cut(s) 160, 730, 779, 790, 894, 1045, 1085
FbaI TGATCA 2 cut(s) 355, 424
FblI GTMKAC 2 cut(s) 75, 687
Fnu4HI GCNGC 1 cut(s) 910
FokI GGATG 6 cut(s) 434, 485, 502, 569, 650, 911
Fsp4HI GCNGC 1 cut(s) 910
FspBI CTAG 1 cut(s) 323
GluI GCNGC 1 cut(s) 910
GsuI CTGGAG 2 cut(s) 576, 635
HaeIII GGCC 1 cut(s) 716
HgaI GACGC 2 cut(s) 79, 86
Hin1I GRCGYC 2 cut(s) 72, 90
Hin1II CATG 7 cut(s) 164, 734, 783, 794, 898, 1049, 1089
HincII GTYRAC 2 cut(s) 76, 688
HindII GTYRAC 2 cut(s) 76, 688
HinfI GANTC 5 cut(s) 434, 452, 746, 871, 1073
HphI GGTGA 2 cut(s) 85, 209
Hpy166II GTNNAC 3 cut(s) 62, 76, 688
Hpy188I TCNGA 1 cut(s) 1020
Hpy188III TCNNGA 6 cut(s) 527, 593, 614, 625, 842, 995
Hpy8I GTNNAC 3 cut(s) 62, 76, 688
Hpy99I CGWCG 3 cut(s) 77, 80, 89
HpyAV CCTTC 3 cut(s) 227, 622, 826
HpyCH4III ACNGT 6 cut(s) 97, 298, 308, 442, 466, 710
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 5 cut(s) 334, 392, 401, 722, 731
HpyF3I CTNAG 3 cut(s) 497, 696, 1071
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 72, 90
Hsp92II CATG 7 cut(s) 164, 734, 783, 794, 898, 1049, 1089
Ksp22I TGATCA 2 cut(s) 355, 424
Kzo9I GATC 5 cut(s) 355, 424, 784, 838, 1048
LmnI GCTCC 3 cut(s) 25, 85, 400
Lsp1109I GCAGC 1 cut(s) 896
LweI GCATC 2 cut(s) 463, 712
MaeI CTAG 1 cut(s) 323
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 91
MalI GATC 5 cut(s) 357, 426, 786, 840, 1050
MboI GATC 5 cut(s) 355, 424, 784, 838, 1048
MboII GAAGA 6 cut(s) 23, 26, 178, 269, 770, 1022
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 4 cut(s) 171, 204, 646, 944
MlyI GAGTC 1 cut(s) 1082
MmeI TCCRAC 3 cut(s) 348, 399, 411
MnlI CCTC 9 cut(s) 36, 193, 210, 494, 529, 589, 610, 859, 960
Mph1103I ATGCAT 3 cut(s) 732, 818, 1091
MseI TTAA 5 cut(s) 138, 149, 185, 570, 645
MslI CAYNNNNRTG 3 cut(s) 467, 795, 899
MspA1I CMGCKG 1 cut(s) 496
MwoI GCNNNNNNNGC 5 cut(s) 334, 392, 401, 722, 731
NdeII GATC 5 cut(s) 355, 424, 784, 838, 1048
NlaIII CATG 7 cut(s) 164, 734, 783, 794, 898, 1049, 1089
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 91
NsiI ATGCAT 3 cut(s) 732, 818, 1091
NspI RCATGY 2 cut(s) 734, 1089
OliI CACNNNNGTG 1 cut(s) 467
PaeI GCATGC 2 cut(s) 734, 1089
PaeR7I CTCGAG 2 cut(s) 623, 863
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 93, 1000
PfeI GAWTC 4 cut(s) 434, 452, 746, 871
PkrI GCNGC 1 cut(s) 911
PleI GAGTC 1 cut(s) 1081
PpsI GAGTC 1 cut(s) 1081
PshBI ATTAAT 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 3 cut(s) 62, 714, 850
PspXI VCTCGAGB 1 cut(s) 863
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 1 cut(s) 769
RsaNI GTAC 1 cut(s) 768
RseI CAYNNNNRTG 3 cut(s) 467, 795, 899
SalI GTCGAC 2 cut(s) 74, 686
SaqAI TTAA 5 cut(s) 138, 149, 185, 570, 645
SatI GCNGC 1 cut(s) 910
Sau3AI GATC 5 cut(s) 355, 424, 784, 838, 1048
Sau96I GGNCC 3 cut(s) 62, 714, 850
SchI GAGTC 1 cut(s) 1082
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 2 cut(s) 463, 712
SfcI CTRYAG 1 cut(s) 539
Sfr274I CTCGAG 2 cut(s) 623, 863
SgrDI CGTCGACG 1 cut(s) 74
SinI GGWCC 2 cut(s) 62, 850
SlaI CTCGAG 2 cut(s) 623, 863
SmiMI CAYNNNNRTG 3 cut(s) 467, 795, 899
SmlI CTYRAG 2 cut(s) 623, 863
SmoI CTYRAG 2 cut(s) 623, 863
SphI GCATGC 2 cut(s) 734, 1089
Sse9I AATT 4 cut(s) 171, 204, 646, 944
SsiI CCGC 2 cut(s) 554, 853
SspI AATATT 1 cut(s) 1063
SspMI CTAG 1 cut(s) 323
TaaI ACNGT 6 cut(s) 97, 298, 308, 442, 466, 710
TaiI ACGT 1 cut(s) 75
TaqI TCGA 8 cut(s) 42, 75, 437, 450, 455, 624, 687, 864
TasI AATT 4 cut(s) 171, 204, 646, 944
TfiI GAWTC 4 cut(s) 434, 452, 746, 871
Tru1I TTAA 5 cut(s) 138, 149, 185, 570, 645
Tru9I TTAA 5 cut(s) 138, 149, 185, 570, 645
TscAI CASTG 4 cut(s) 109, 301, 469, 969
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 909
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 4 cut(s) 504, 728, 920, 1037
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 4 cut(s) 109, 301, 469, 969
VpaK11BI GGWCC 2 cut(s) 62, 850
VspI ATTAAT 1 cut(s) 645
XceI RCATGY 2 cut(s) 734, 1089
XcmI CCANNNNNNNNNTGG 1 cut(s) 996
XhoI CTCGAG 2 cut(s) 623, 863
XmiI GTMKAC 2 cut(s) 75, 687
XspI CTAG 1 cut(s) 323
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 3 cut(s) 732, 818, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.