FvH4_4g30300

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011931
Physical Location & Seq
Reverse (-)
30003607 .. 30006455
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30300.t1

Sequence Viewer

Length: 1101 bp
ATGCAGGACGATGAAGAAGCTCCACCTCTCGCCGTTGAAATCGACCAACCTTTTGCAAGTGGGTCCAATGACGTCGACGCTCCCGTCGGCGTCACCGTCATCACCGGTTATCTGGGTTCAGGCAAATCCACTCTGGTTAATCATATATTGAACACCCAACATGGGAAGAAGATTGCTGTGATATTAAACGAGTTTGGTGAGGAAGTTGGGGTTGAAAGAGCAATGATAAACGAAGGAGATGGTGGTGCTCTTGTGGAAGAGTGGGTTGAGTTGGCAAATGGGTGTATATGTTGTACTGTGAAGCACAGTTTGGTGCAAGCATTAGAGCAACTTGTGGAGAGAAAAGAAAGACTTGATCATATATTGCTGGAGACTACTGGGTTGGCCAACCCTGCCCCGCTTGCTTCGGTGCTGTGGTTGGATGATCAGTTGGAATCGTCTGTCAAGCTTGATTCGATTATCACTGTTGTGGATGCCAAGAACCTCCGTTTTCAGCTCAGTGAGAATAAAAGTTCATCCTCTTTTCCTGAAGCATATTTACAGATTGCATTTGCGGATGTTGTAATTCTTAACAAGGTTGATTTGGTTACTACAGAGGGTTCTACAGATTATCTGGAGGAACTTGAGAAGGAGATACATAGTATTAATTCGCTGGCTTGTATCATCCGTTCTATTCGTAGTCAAGTCGACTTGTCTAAAATCTTGGACTGTCGAGCCTATGATGCTGCGCATGCTGCTCATTTAGAATCATTATTGGAAGAAAGTCGTGCTTTATCTACTCTGGATCTCCATGATAGTGGTGTGCGAACCTTATGCATTTGCGAGCAACAGAAGGTTGATCTTGAAAAGGTCCGTTTATGGCTCGAGGAGATTCTTTGGGAGAAGAAATATGGCATGGATGTGTATCGCTGCAAAGGGGTTTTGAGCGTCCACAATTCAAACCAATTACATACTTTGCAGGCAGTCAGAGAAATATATGAGATTGTGCCAACTCGTGTTTGGAAAAAGGAAGAAAATCAGATGAACAAGATAGTGTTTATAGGGCATAATATAAACGAGAATGTTCTTACCCAGTTGTTTAGAGCCTGCGTAGTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C KTI12 cobW

Protein Analysis

367

Amino Acids

40.8

Weight (kDa)

4.83

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 31 - 219 3e-57 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 269 - 364 6.4e-18 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011931)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26520
fragaria_vesca FvH4_4g30300
malus_domestica MD13G1064000.v1.1 MD16G1062700.v1.1
prunus_persica Prupe.1G284700_v2.0.a1
pyrus_communis pycom16g05480
rosa_chinensis RchiOBHm_Chr4g0438581
rosa_laevigata RLG00000006349
rosa_multiflora Rmu_co8071018.1_g000001 Rmu_sc0002327.1_g000002 Rmu_sc0020430.1_g000001
rosa_roxburghii Rroxscaffold_5G00379590
rosa_rugosa Rorug04G0311700
rosa_samantha Rh4AG362100 Rh4BG373500 Rh4CG387400 Rh4DG368200
rosa_wichuraiana Rw0G006680 Rw0G016630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AatII GACGTC 1 cut(s) 75
Acc16I TGCGCA 1 cut(s) 729
AccI GTMKAC 2 cut(s) 75, 687
AciI CCGC 2 cut(s) 398, 554
AclWI GGATC 1 cut(s) 792
AcoI YGGCCR 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 2 cut(s) 72, 90
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 1 cut(s) 162
AgeI ACCGGT 1 cut(s) 104
AgsI TTSAA 5 cut(s) 38, 151, 215, 845, 939
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 3 cut(s) 20, 448, 496
AluI AGCT 3 cut(s) 20, 448, 496
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 792
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 3 cut(s) 725, 734, 909
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AseI ATTAAT 1 cut(s) 645
AsiGI ACCGGT 1 cut(s) 104
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 2 cut(s) 63, 850
AsuHPI GGTGA 3 cut(s) 85, 94, 209
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 2 cut(s) 63, 850
BalI TGGCCA 1 cut(s) 386
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BauI CACGAG 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 712, 721, 896
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 17
BcgI CGANNNNNNTGC 2 cut(s) 795, 829
BclI TGATCA 2 cut(s) 355, 424
BcoDI GTCTC 1 cut(s) 365
BfmI CTRYAG 2 cut(s) 591, 603
BisI GCNGC 3 cut(s) 726, 735, 910
BlsI GCNGC 3 cut(s) 727, 736, 911
Bme18I GGWCC 2 cut(s) 63, 850
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 2 cut(s) 63, 850
BmiI GGNNCC 1 cut(s) 64
BmrI ACTGGG 2 cut(s) 387, 1066
BmsI GCATC 2 cut(s) 463, 712
BmuI ACTGGG 2 cut(s) 387, 1066
BpmI CTGGAG 2 cut(s) 389, 635
BpuEI CTTGAG 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 903
BsaHI GRCGYC 2 cut(s) 72, 90
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 382, 1072
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 903
BseGI GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 511
BseNI ACTGG 2 cut(s) 382, 1072
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 3 cut(s) 712, 721, 896
BshFI GGCC 1 cut(s) 386
BshTI ACCGGT 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 863
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 4 cut(s) 355, 424, 784, 838
BspACI CCGC 2 cut(s) 398, 554
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 64
BspPI GGATC 1 cut(s) 792
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 382, 1072
BssAI RCCGGY 1 cut(s) 104
BssMI GATC 4 cut(s) 355, 424, 784, 838
BssNI GRCGYC 2 cut(s) 72, 90
BssSI CACGAG 1 cut(s) 993
Bst2BI CACGAG 1 cut(s) 993
Bst4CI ACNGT 5 cut(s) 97, 298, 308, 466, 710
Bst6I CTCTTC 1 cut(s) 252
BstACI GRCGYC 2 cut(s) 72, 90
BstC8I GCNNGC 7 cut(s) 318, 402, 654, 732, 824, 960, 1087
BstDEI CTNAG 1 cut(s) 497
BstF5I GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 4 cut(s) 358, 427, 787, 841
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 4 cut(s) 355, 424, 784, 838
BstMWI GCNNNNNNNGC 5 cut(s) 392, 401, 722, 731, 734
BstNSI RCATGY 1 cut(s) 734
BstSFI CTRYAG 2 cut(s) 591, 603
BstV1I GCAGC 3 cut(s) 712, 721, 896
BstX2I RGATCY 1 cut(s) 784
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 1 cut(s) 784
BsuRI GGCC 1 cut(s) 386
BtsCI GGATG 6 cut(s) 427, 478, 515, 562, 663, 904
BtsIMutI CAGTG 2 cut(s) 462, 505
Cac8I GCNNGC 7 cut(s) 318, 402, 654, 732, 824, 960, 1087
CfoI GCGC 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 63, 850
CseI GACGC 3 cut(s) 79, 86, 916
Csp6I GTAC 1 cut(s) 294
CspAI ACCGGT 1 cut(s) 104
CviAII CATG 4 cut(s) 161, 731, 791, 895
CviJI RGCY 8 cut(s) 20, 386, 448, 496, 656, 716, 862, 1085
CviKI_1 RGCY 8 cut(s) 20, 386, 448, 496, 656, 716, 862, 1085
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 1 cut(s) 497
DpnI GATC 4 cut(s) 357, 426, 786, 840
DpnII GATC 4 cut(s) 355, 424, 784, 838
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 63, 850
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 863
EcoT22I ATGCAT 1 cut(s) 818
FaeI CATG 4 cut(s) 164, 734, 794, 898
FalI AAGNNNNNCTT 4 cut(s) 336, 368, 754, 786
FatI CATG 4 cut(s) 160, 730, 790, 894
FauI CCCGC 1 cut(s) 405
FbaI TGATCA 2 cut(s) 355, 424
FblI GTMKAC 2 cut(s) 75, 687
Fnu4HI GCNGC 3 cut(s) 726, 735, 910
FokI GGATG 6 cut(s) 434, 485, 502, 569, 650, 911
Fsp4HI GCNGC 3 cut(s) 726, 735, 910
FspI TGCGCA 1 cut(s) 729
GlaI GCGC 1 cut(s) 729
GluI GCNGC 3 cut(s) 726, 735, 910
GsuI CTGGAG 2 cut(s) 389, 635
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 105
HgaI GACGC 3 cut(s) 79, 86, 916
HhaI GCGC 1 cut(s) 730
Hin1I GRCGYC 2 cut(s) 72, 90
Hin1II CATG 4 cut(s) 164, 734, 794, 898
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HincII GTYRAC 2 cut(s) 76, 688
HindII GTYRAC 2 cut(s) 76, 688
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 434, 452, 746, 871
HpaII CCGG 1 cut(s) 105
HphI GGTGA 3 cut(s) 85, 94, 209
Hpy166II GTNNAC 3 cut(s) 76, 688, 931
Hpy188I TCNGA 2 cut(s) 968, 1020
Hpy188III TCNNGA 4 cut(s) 527, 614, 782, 842
Hpy8I GTNNAC 3 cut(s) 76, 688, 931
Hpy99I CGWCG 3 cut(s) 77, 80, 89
HpyAV CCTTC 3 cut(s) 227, 622, 826
HpyCH4III ACNGT 5 cut(s) 97, 298, 308, 466, 710
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 7 cut(s) 4, 56, 316, 548, 816, 912, 958
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 401, 722, 731, 734
HpyF3I CTNAG 1 cut(s) 497
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 72, 90
Hsp92II CATG 4 cut(s) 164, 734, 794, 898
HspAI GCGC 1 cut(s) 728
Ksp22I TGATCA 2 cut(s) 355, 424
Kzo9I GATC 4 cut(s) 355, 424, 784, 838
LmnI GCTCC 2 cut(s) 25, 85
Lsp1109I GCAGC 3 cut(s) 712, 721, 896
LweI GCATC 2 cut(s) 463, 712
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 91, 586
MalI GATC 4 cut(s) 357, 426, 786, 840
MboI GATC 4 cut(s) 355, 424, 784, 838
MboII GAAGA 7 cut(s) 26, 178, 181, 269, 770, 895, 1022
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 1 cut(s) 250
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 4 cut(s) 564, 646, 934, 944
MluNI TGGCCA 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 399, 411
MnlI CCTC 7 cut(s) 36, 193, 494, 529, 589, 610, 859
Mox20I TGGCCA 1 cut(s) 386
Mph1103I ATGCAT 1 cut(s) 818
MscI TGGCCA 1 cut(s) 386
MseI TTAA 5 cut(s) 138, 185, 570, 645, 1099
MslI CAYNNNNRTG 3 cut(s) 467, 795, 899
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 1 cut(s) 105
MwoI GCNNNNNNNGC 5 cut(s) 392, 401, 722, 731, 734
NdeII GATC 4 cut(s) 355, 424, 784, 838
NlaIII CATG 4 cut(s) 164, 734, 794, 898
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 91
NsbI TGCGCA 1 cut(s) 729
NsiI ATGCAT 1 cut(s) 818
NspI RCATGY 1 cut(s) 734
OliI CACNNNNGTG 1 cut(s) 467
PaeI GCATGC 1 cut(s) 734
PaeR7I CTCGAG 1 cut(s) 863
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 93
PfeI GAWTC 4 cut(s) 434, 452, 746, 871
PinAI ACCGGT 1 cut(s) 104
PkrI GCNGC 3 cut(s) 727, 736, 911
PshBI ATTAAT 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 2 cut(s) 63, 850
PspXI VCTCGAGB 1 cut(s) 863
PsrI GAACNNNNNNTAC 2 cut(s) 652, 684
PsuI RGATCY 1 cut(s) 784
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
RseI CAYNNNNRTG 3 cut(s) 467, 795, 899
SalI GTCGAC 2 cut(s) 74, 686
SaqAI TTAA 5 cut(s) 138, 185, 570, 645, 1099
SatI GCNGC 3 cut(s) 726, 735, 910
Sau3AI GATC 4 cut(s) 355, 424, 784, 838
Sau96I GGNCC 2 cut(s) 63, 850
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 2 cut(s) 463, 712
SfcI CTRYAG 2 cut(s) 591, 603
Sfr274I CTCGAG 1 cut(s) 863
SgrDI CGTCGACG 1 cut(s) 74
SinI GGWCC 2 cut(s) 63, 850
SlaI CTCGAG 1 cut(s) 863
SmiMI CAYNNNNRTG 3 cut(s) 467, 795, 899
SmlI CTYRAG 2 cut(s) 623, 863
SmoI CTYRAG 2 cut(s) 623, 863
SphI GCATGC 1 cut(s) 734
Sse9I AATT 4 cut(s) 564, 646, 934, 944
SsiI CCGC 2 cut(s) 398, 554
TaaI ACNGT 5 cut(s) 97, 298, 308, 466, 710
TaiI ACGT 1 cut(s) 75
TaqI TCGA 6 cut(s) 42, 75, 455, 687, 712, 864
TasI AATT 4 cut(s) 564, 646, 934, 944
TatI WGTACW 1 cut(s) 293
TfiI GAWTC 4 cut(s) 434, 452, 746, 871
Tru1I TTAA 5 cut(s) 138, 185, 570, 645, 1099
Tru9I TTAA 5 cut(s) 138, 185, 570, 645, 1099
TscAI CASTG 2 cut(s) 469, 505
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 725, 734, 909
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 27, 504, 1037
TspGWI ACGGA 3 cut(s) 476, 656, 842
TspRI CASTG 2 cut(s) 469, 505
VpaK11BI GGWCC 2 cut(s) 63, 850
VspI ATTAAT 1 cut(s) 645
XceI RCATGY 1 cut(s) 734
XcmI CCANNNNNNNNNTGG 1 cut(s) 996
XhoI CTCGAG 1 cut(s) 863
XmiI GTMKAC 2 cut(s) 75, 687
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.